##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546282_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2208954 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.120116580064593 31.0 31.0 33.0 30.0 34.0 2 31.392451359331158 31.0 31.0 34.0 30.0 34.0 3 31.431186434846538 31.0 31.0 34.0 30.0 34.0 4 35.25660923676998 37.0 35.0 37.0 33.0 37.0 5 35.00556824632835 37.0 35.0 37.0 32.0 37.0 6 35.14867217696702 37.0 35.0 37.0 32.0 37.0 7 35.66920225590936 37.0 35.0 37.0 35.0 37.0 8 35.71496554477821 37.0 35.0 37.0 35.0 37.0 9 37.397594970289106 39.0 37.0 39.0 35.0 39.0 10 36.73893888238506 39.0 37.0 39.0 32.0 39.0 11 36.49045973795742 39.0 35.0 39.0 32.0 39.0 12 35.9966862143802 38.0 35.0 39.0 31.0 39.0 13 35.70922481862456 37.0 35.0 39.0 30.0 39.0 14 36.71962295276407 39.0 35.0 41.0 31.0 41.0 15 36.86533535782094 39.0 35.0 41.0 31.0 41.0 16 36.914180195694435 39.0 35.0 41.0 31.0 41.0 17 36.83031063571265 38.0 35.0 41.0 31.0 41.0 18 36.722874265376284 38.0 35.0 40.0 31.0 41.0 19 36.622659865257496 38.0 35.0 40.0 31.0 41.0 20 36.512964507183035 38.0 35.0 40.0 31.0 41.0 21 36.38738063354873 38.0 35.0 40.0 30.0 41.0 22 36.29963774709659 38.0 35.0 40.0 30.0 41.0 23 36.240735660407594 38.0 35.0 40.0 30.0 41.0 24 36.12544127220395 38.0 34.0 40.0 30.0 41.0 25 35.97983163071753 38.0 34.0 40.0 30.0 41.0 26 35.94438589486246 38.0 34.0 40.0 30.0 41.0 27 35.88872289780593 38.0 34.0 40.0 30.0 41.0 28 35.81940230534452 38.0 34.0 40.0 29.0 41.0 29 35.76999113607617 38.0 34.0 40.0 29.0 41.0 30 35.60042354888332 37.0 34.0 40.0 29.0 41.0 31 35.45010353316547 38.0 34.0 40.0 28.0 41.0 32 35.20997766363627 37.0 34.0 40.0 26.0 41.0 33 34.921595470073164 37.0 34.0 40.0 25.0 41.0 34 34.6236757306852 37.0 33.0 40.0 24.0 41.0 35 34.36588267569175 37.0 33.0 40.0 23.0 41.0 36 34.227353308398456 37.0 33.0 40.0 23.0 41.0 37 34.18748602279631 37.0 33.0 40.0 23.0 41.0 38 34.110942554711414 37.0 33.0 40.0 23.0 41.0 39 33.99919690496045 37.0 33.0 40.0 22.0 41.0 40 33.88079606908971 37.0 33.0 40.0 21.0 41.0 41 33.74912832046299 36.0 33.0 40.0 21.0 41.0 42 33.66627009887938 36.0 33.0 40.0 21.0 41.0 43 33.63154959315585 36.0 33.0 40.0 21.0 41.0 44 33.38299892166157 36.0 32.0 40.0 19.0 41.0 45 33.3704671985021 35.0 32.0 40.0 20.0 41.0 46 33.31798941942657 35.0 32.0 40.0 20.0 41.0 47 33.26877200702233 35.0 32.0 40.0 20.0 41.0 48 33.14028313853525 35.0 32.0 39.0 20.0 41.0 49 33.04723683698257 35.0 31.0 39.0 20.0 41.0 50 33.00477329994197 35.0 31.0 39.0 20.0 41.0 51 32.92530582348025 35.0 31.0 39.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 2.0 12 4.0 13 19.0 14 26.0 15 76.0 16 333.0 17 763.0 18 1763.0 19 3431.0 20 5847.0 21 9010.0 22 13077.0 23 18752.0 24 25770.0 25 36434.0 26 47456.0 27 53725.0 28 55507.0 29 59936.0 30 68720.0 31 80852.0 32 97238.0 33 121663.0 34 182270.0 35 224851.0 36 177934.0 37 220776.0 38 302379.0 39 400227.0 40 112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.51602342104 23.064717508829972 27.23347792665669 17.185781143473335 2 34.35979201015503 24.76076912420992 28.333274481949378 12.54616438368567 3 28.326076504988336 24.75787182530736 34.04131548235047 12.874736187353832 4 25.618460139957644 26.58203837653478 33.32495832869313 14.474543154814452 5 22.717811235544065 30.266180282613398 32.46427947345214 14.551729008390396 6 21.12565494799801 38.91402899290796 29.656072512148285 10.304243546945749 7 82.38808956637395 4.62205188519091 10.7135775575227 2.276280990912441 8 83.17167310862969 3.98704545228194 10.518236233076832 2.323045206011533 9 78.13390410121713 5.850732971352052 12.322574394939867 3.692788532490944 10 41.05830180257262 26.835370949327146 19.15291128742382 12.95341596067641 11 34.59727997957404 23.225245976149797 26.207788844855983 15.969685199420178 12 31.54081071855729 21.191251605963725 30.631647376993815 16.636290298485164 13 24.322145232539928 26.99268522567695 30.391895892807185 18.29327364897594 14 19.947088078792046 29.00952215392444 32.046072484986105 18.997317282297413 15 19.304340425377802 23.078660759798527 39.45795159156778 18.159047223255893 16 22.56656317877149 20.647645899371376 36.4838742680925 20.301916653764632 17 22.10752238389754 21.3894449590168 31.313146403229762 25.189886253855896 18 24.032007909626003 22.701876091579997 33.03907641354234 20.227039585251664 19 26.58194783594407 24.756242094674676 28.143954106785387 20.51785596259587 20 28.2344041568996 23.501168426323048 28.70562266122337 19.558804755553986 21 25.1320760866908 25.25638831772866 30.076814637154055 19.534720958426476 22 24.208697872386658 22.954393799056025 29.987496344423647 22.849411984133667 23 22.209380548440574 25.312432943375008 30.214798497388358 22.26338801079606 24 22.34211305441399 23.39600553021928 34.477540048366784 19.784341366999946 25 22.26637585028932 24.594174437312862 31.351807235460765 21.787642476937048 26 20.745475007628045 26.39353286668713 30.042952456230417 22.81803966945441 27 20.158455087792685 26.37424772086698 31.530036388263404 21.93726080307693 28 19.488002013622737 25.63109055236098 33.53179830815852 21.34910912585776 29 20.997721093331954 24.113947144213956 32.90453309575482 21.98379866669926 30 22.383082671707967 25.39763163922834 31.966532576051833 20.25275311301186 31 25.62004460029498 24.890061087736548 28.871583563985485 20.61831074798298 32 26.733648595670168 24.07392820312238 28.398146815189452 20.794276386017998 33 26.208558439876974 25.140043658672838 27.91873438740689 20.7326635140433 34 22.40834349651464 25.23868763224585 30.309594495856413 22.0433743753831 35 22.282446805139447 25.865319060514615 30.08446531706862 21.767768817277318 36 25.23646938777358 27.688082232586105 26.950991283657334 20.124457095982986 37 22.888887681681013 29.06366542716598 27.963144547147657 20.08430234400535 38 22.828587648271533 28.550617169936537 27.558790269059475 21.06200491273245 39 22.457507037267412 28.32580488321622 27.460780079621394 21.755907999894973 40 24.594219707608218 25.8108588952056 27.437375336924173 22.157546060262007 41 21.01560285999618 25.175173407866346 28.48348132192884 25.325742410208633 42 21.714666760828884 25.709317622730033 27.289386741416976 25.286628875024107 43 21.83295804258486 25.279521438653767 28.27759201866585 24.60992850009552 44 22.052292623567535 26.366280148884947 28.42662183096615 23.154805396581366 45 21.10718466749421 28.62295910190977 26.630024889608382 23.639831340987634 46 22.358048198378057 28.207966304413763 27.498218613877878 21.935766883330302 47 21.5163828671851 27.204278586154352 29.092502605305498 22.18683594135505 48 21.809643840478344 25.63951082729654 30.30407151982341 22.246773812401706 49 22.333556968592376 25.044478065183796 30.247710002109596 22.374254964114236 50 21.06123531771146 27.19622047358161 29.470917004156718 22.271627204550207 51 20.546783681326094 27.32175500259399 28.97529781063798 23.156163505441942 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 895.0 1 1754.5 2 2614.0 3 18897.0 4 35180.0 5 25194.0 6 15208.0 7 15146.5 8 15085.0 9 15478.0 10 15871.0 11 15763.5 12 15656.0 13 15236.0 14 14816.0 15 14156.0 16 13496.0 17 12977.0 18 12458.0 19 12403.5 20 12349.0 21 12101.5 22 11854.0 23 12048.0 24 12242.0 25 13872.5 26 18189.0 27 20875.0 28 23379.5 29 25884.0 30 29964.0 31 34044.0 32 40774.5 33 47505.0 34 55678.0 35 63851.0 36 66438.5 37 69026.0 38 75818.0 39 82610.0 40 96645.0 41 110680.0 42 121311.5 43 131943.0 44 138549.5 45 145156.0 46 147064.5 47 148973.0 48 147029.5 49 145086.0 50 146567.0 51 148048.0 52 139738.5 53 131429.0 54 128897.0 55 126365.0 56 122403.0 57 118441.0 58 115954.5 59 113468.0 60 108439.0 61 103410.0 62 91373.5 63 79337.0 64 69719.0 65 60101.0 66 50845.5 67 41590.0 68 34455.5 69 27321.0 70 22234.5 71 17148.0 72 13812.0 73 10476.0 74 8271.0 75 4839.5 76 3613.0 77 2639.0 78 1665.0 79 1336.5 80 1008.0 81 684.0 82 360.0 83 238.0 84 116.0 85 99.0 86 82.0 87 54.0 88 26.0 89 19.5 90 13.0 91 12.0 92 11.0 93 5.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2208954.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.239810470716414 #Duplication Level Percentage of deduplicated Percentage of total 1 78.34790309536533 18.991383218097113 2 5.997207511482122 2.907423468637669 3 2.356109336881401 1.7133493132287145 4 1.4307983294162052 1.3872912130746593 5 1.0065306505856848 1.2199056101581947 6 0.758259438221979 1.1028039042079603 7 0.618399787462825 1.0492925550261134 8 0.4983969842981473 0.9664838750850971 9 0.45492111323924833 0.9924481407642028 >10 6.9752537806356 40.72098060837037 >50 1.4216721300045643 23.130788556167293 >100 0.13132176612261126 4.268947882508014 >500 0.002656768704701315 0.38423543363914014 >1k 3.795383863859022E-4 0.2615542530434755 >5k 0.0 0.0 >10k+ 1.897691931929511E-4 0.9031119679920074 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19633 0.8887917086548656 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 2956 0.13381899306187453 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 2730 0.12358790631221836 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.527029535246094E-5 0.0 0.0 0.04101488758932961 0.0 2 4.527029535246094E-5 0.0 0.0 0.10738114057603734 0.0 3 4.527029535246094E-5 0.0 0.0 0.15704265457768699 0.0 4 4.527029535246094E-5 0.0 0.0 0.23019945186726387 0.0 5 4.527029535246094E-5 0.0 0.0 0.38104007598166373 0.0 6 4.527029535246094E-5 0.0 0.0 0.6012800628713861 0.0 7 4.527029535246094E-5 0.0 0.0 0.7615821787144503 0.0 8 4.527029535246094E-5 0.0 0.0 1.0636708596014222 0.0 9 4.527029535246094E-5 0.0 0.0 1.2322121691986343 0.0 10 4.527029535246094E-5 0.0 0.0 1.4720541939759724 0.0 11 4.527029535246094E-5 0.0 0.0 1.6495590220529717 0.0 12 4.527029535246094E-5 0.0 0.0 1.8162895198360853 0.0 13 4.527029535246094E-5 0.0 0.0 1.8834706381391373 0.0 14 4.527029535246094E-5 0.0 0.0 1.9205017397374504 0.0 15 4.527029535246094E-5 0.0 0.0 1.9611997352593127 0.0 16 4.527029535246094E-5 2.263514767623047E-4 0.0 2.0327720722115536 0.0 17 4.527029535246094E-5 2.263514767623047E-4 0.0 2.1124025217365325 0.0 18 4.527029535246094E-5 2.263514767623047E-4 0.0 2.2256688007083896 0.0 19 4.527029535246094E-5 2.263514767623047E-4 0.0 2.284429644075884 0.0 20 4.527029535246094E-5 2.263514767623047E-4 0.0 2.3507053564718867 0.0 21 4.527029535246094E-5 2.263514767623047E-4 0.0 2.429611481271226 0.0 22 4.527029535246094E-5 2.263514767623047E-4 0.0 2.514764906829205 0.0 23 9.054059070492188E-5 2.263514767623047E-4 0.0 2.614042664537152 0.0 24 9.054059070492188E-5 2.263514767623047E-4 0.0 2.6950312229227045 0.0 25 9.054059070492188E-5 2.263514767623047E-4 0.0 2.766784641056355 0.0 26 9.054059070492188E-5 2.263514767623047E-4 0.0 2.8366367067852023 0.0 27 9.054059070492188E-5 2.263514767623047E-4 0.0 2.91219282972846 0.0 28 9.054059070492188E-5 2.263514767623047E-4 0.0 2.991868549548791 0.0 29 9.054059070492188E-5 2.263514767623047E-4 0.0 3.083405086751467 0.0 30 9.054059070492188E-5 2.263514767623047E-4 0.0 3.2053632624309967 0.0 31 9.054059070492188E-5 2.263514767623047E-4 0.0 3.295722771954509 0.0 32 9.054059070492188E-5 2.263514767623047E-4 0.0 3.390428229831857 0.0 33 9.054059070492188E-5 2.263514767623047E-4 0.0 3.482055307625238 0.0 34 9.054059070492188E-5 2.263514767623047E-4 0.0 3.5797938752912013 0.0 35 1.358108860573828E-4 2.263514767623047E-4 0.0 3.7006202935869195 0.0 36 1.358108860573828E-4 2.263514767623047E-4 0.0 3.799264267159932 0.0 37 1.358108860573828E-4 2.263514767623047E-4 0.0 3.906464326554559 0.0 38 2.263514767623047E-4 2.263514767623047E-4 0.0 4.040238049321081 0.0 39 2.263514767623047E-4 2.263514767623047E-4 0.0 4.235805725243712 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGAC 30 2.1664146E-6 44.999996 23 CGTTTTT 15355 0.0 43.476067 1 CGCATCG 120 0.0 39.374996 21 CGTTCGA 75 0.0 39.0 14 CGTACCG 35 6.250906E-6 38.571426 41 CGTAAGG 345 0.0 37.826088 2 ATCGACG 30 1.1402627E-4 37.499996 1 GTTTTTT 18725 0.0 36.949265 2 TAAGGGA 2000 0.0 36.7875 4 CGGTCTA 215 0.0 36.627907 31 AGGGCGA 2015 0.0 36.62531 6 GGGCGAT 3770 0.0 36.465515 7 TCACGAC 210 0.0 36.42857 25 ATTCGGC 210 0.0 36.42857 17 ATACTAT 1850 0.0 36.364864 45 TATTGCG 180 0.0 36.250004 1 CTCGATC 305 0.0 36.14754 16 AACGGGA 650 0.0 36.0 4 TACGATA 25 0.002107528 36.0 23 GTAGGGA 2150 0.0 35.999996 4 >>END_MODULE