FastQCFastQC Report
Thu 26 May 2016
SRR1546281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546281_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1585945
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC63090.397806985740363No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36120.22775064709053594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG302.1660253E-645.0000041
GACCGGC302.1660253E-645.00000433
TACGGGT302.1660253E-645.0000044
CGTGTCA302.1660253E-645.00000436
ATACGTC302.1660253E-645.00000437
GCGCTAA302.1660253E-645.00000420
TCGACTA302.1660253E-645.0000041
TACGTCT302.1660253E-645.00000438
CGGGTAT351.21232E-745.06
AAATCGT207.0340995E-445.012
CGTATTA207.0340995E-445.031
TTAGCGA453.8562575E-1045.027
ACTCTCG207.0340995E-445.043
TATGCGA207.0340995E-445.013
ACGGGTA207.0340995E-445.05
AGTATCG453.8562575E-1045.01
TATCTCG253.8914783E-545.01
CGCTATA207.0340995E-445.031
ACGTGTA253.8914783E-545.010
CCGAGTA253.8914783E-545.017