FastQCFastQC Report
Thu 26 May 2016
SRR1546279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546279_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2206489
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC63600.2882407299560524No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52500.23793456482221303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA253.8919767E-545.010
GTCGATA207.034701E-445.044
ACGTTGA253.8919767E-545.014
CGTCTAG351.2126111E-745.018
CATTACG207.034701E-445.039
CTACGAT207.034701E-445.041
TTAACGC253.8919767E-545.028
ACTCGAC253.8919767E-545.035
TCCAACG253.8919767E-545.027
CGACTCA207.034701E-445.020
CCCGGTA207.034701E-445.019
CGATCGC253.8919767E-545.034
TAATGCG900.045.01
CGATACA453.8562575E-1045.010
CCGTTCG207.034701E-445.036
TACCGAA253.8919767E-545.030
CGTTTAC950.042.6315833
CGTTTTT57200.041.8924831
CACGACG2650.041.60377526
CGGTCTA2600.041.5384631