##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546279_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2206489
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275409938594755	31.0	31.0	33.0	30.0	34.0
2	31.509537097171116	31.0	31.0	34.0	30.0	34.0
3	31.59270134589386	31.0	31.0	34.0	30.0	34.0
4	35.36481487104626	37.0	35.0	37.0	33.0	37.0
5	35.09584865367559	37.0	35.0	37.0	32.0	37.0
6	35.24034926074864	37.0	35.0	37.0	32.0	37.0
7	35.76642439640533	37.0	35.0	37.0	35.0	37.0
8	35.83681087918408	37.0	35.0	37.0	35.0	37.0
9	37.578243535317874	39.0	37.0	39.0	35.0	39.0
10	36.874174763617674	39.0	37.0	39.0	32.0	39.0
11	36.556135108763286	39.0	35.0	39.0	32.0	39.0
12	35.678455229099264	37.0	35.0	39.0	31.0	39.0
13	35.19945125491222	37.0	35.0	39.0	30.0	39.0
14	36.16745064217406	38.0	35.0	40.0	30.0	41.0
15	36.454196689854335	38.0	35.0	40.0	31.0	41.0
16	36.610788904907295	38.0	35.0	40.0	31.0	41.0
17	36.541665514761235	38.0	35.0	40.0	31.0	41.0
18	36.50330457119886	38.0	35.0	40.0	31.0	41.0
19	36.46558763719194	38.0	35.0	40.0	31.0	41.0
20	36.37857700627558	38.0	35.0	40.0	31.0	41.0
21	36.24419201727269	38.0	35.0	40.0	31.0	41.0
22	36.144432625768815	37.0	35.0	40.0	30.0	41.0
23	36.091775213925835	37.0	34.0	40.0	30.0	41.0
24	35.9958150709113	37.0	34.0	40.0	30.0	41.0
25	35.88860492846327	37.0	34.0	40.0	30.0	41.0
26	35.83312629249455	37.0	34.0	40.0	30.0	41.0
27	35.75595708838793	37.0	34.0	40.0	30.0	41.0
28	35.75740463695944	37.0	34.0	40.0	30.0	41.0
29	35.80223105576325	37.0	34.0	40.0	30.0	41.0
30	35.716381092314535	37.0	34.0	40.0	30.0	41.0
31	35.62000173125721	37.0	34.0	40.0	30.0	41.0
32	35.44875999835032	37.0	34.0	40.0	29.0	41.0
33	35.27655927584502	37.0	34.0	40.0	29.0	41.0
34	35.13279830536205	37.0	34.0	40.0	27.0	41.0
35	34.9759735942486	36.0	34.0	40.0	27.0	41.0
36	34.83135605933227	36.0	34.0	40.0	26.0	41.0
37	34.74883491374759	36.0	33.0	40.0	26.0	41.0
38	34.76149439222221	36.0	33.0	40.0	26.0	41.0
39	34.69606012085263	36.0	34.0	40.0	26.0	41.0
40	34.54221435049076	36.0	33.0	40.0	25.0	41.0
41	34.49913550441448	36.0	33.0	40.0	25.0	41.0
42	34.446118244867755	36.0	33.0	40.0	25.0	41.0
43	34.4229225706541	35.0	33.0	40.0	25.0	41.0
44	34.21200332292615	35.0	33.0	40.0	24.0	41.0
45	34.196289217847905	35.0	33.0	40.0	24.0	41.0
46	34.1525015533728	35.0	33.0	40.0	24.0	41.0
47	34.10838395296781	35.0	33.0	40.0	24.0	41.0
48	34.05141652643634	35.0	33.0	39.0	24.0	41.0
49	33.982394655037936	35.0	33.0	39.0	24.0	41.0
50	33.91705827674645	35.0	33.0	39.0	24.0	41.0
51	33.848803234459815	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	6.0
13	11.0
14	17.0
15	65.0
16	209.0
17	476.0
18	1087.0
19	2264.0
20	3893.0
21	6475.0
22	10454.0
23	15097.0
24	20677.0
25	27456.0
26	34585.0
27	40244.0
28	45007.0
29	53246.0
30	64285.0
31	79103.0
32	99501.0
33	130373.0
34	220609.0
35	309218.0
36	158447.0
37	194733.0
38	276882.0
39	411925.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.97800759487131	24.136762068607638	28.769189422652914	13.11604091386814
2	29.682812830700723	26.119323504445298	30.591768189191065	13.606095475662919
3	29.050994589141393	26.0030301533341	30.683497629038715	14.262477628485797
4	26.403122789191336	27.812511188589657	30.704299908134598	15.080066114084412
5	23.78906035788078	32.13983844922861	29.91403990683842	14.157061286052187
6	21.909241333176826	40.45336278585572	26.90745342487545	10.72994245609201
7	87.00986046157493	4.595354882802498	6.496791962253154	1.897992693369421
8	88.56839984246466	4.122748855761348	5.576778311607264	1.732072990166731
9	84.22860934271597	5.199618035711938	7.256868264469027	3.314904357103072
10	51.661576377675125	22.64334877717496	14.390010555230504	11.305064289919416
11	46.07360381130384	21.99911261737539	18.27477952530015	13.652504046020624
12	41.46261322852731	21.96145097482924	22.201968829212383	14.373966967431064
13	23.39064459419467	38.59158146720876	22.32768892117749	15.690085017419076
14	16.610551876759867	40.747540549714955	26.03606906719227	16.605838506332912
15	15.931328005714054	24.66352653468927	44.35653202893828	15.048613430658389
16	17.449350529279776	18.90356126860365	43.77642489946698	19.870663302649593
17	17.622566892470346	20.127496670049112	28.351285685086125	33.89865075239442
18	21.83709050894883	24.20710005805603	32.24321535253518	21.71259408045995
19	28.91018264763613	25.959658081232217	24.387295835148056	20.74286343598359
20	32.367077288851206	23.11772231812622	24.96445710810251	19.55074328492007
21	23.626449078150856	27.592070479390564	27.2358031243301	21.545677318128483
22	23.54786269045529	26.321726507587396	24.877712963898755	25.252697838058562
23	21.96407958525966	30.40459299819759	23.686635192833503	23.94469222370925
24	21.412705887044982	25.3399858326962	34.48369785664012	18.763610423618697
25	19.264587314960554	26.297343879801804	32.9994847017139	21.43858410352374
26	18.51375647012063	33.1383025249616	27.40081641014299	20.947124594774778
27	19.62416309349378	33.28854120732077	27.601678503722432	19.485617195463018
28	17.1562604662883	29.33488451562641	35.36391978387384	18.144935234211456
29	18.17144794286307	25.469059669003563	35.56904203918533	20.790450348948035
30	19.572950510970145	29.74766699494083	30.78365675061149	19.895725743477534
31	27.51176189865438	27.961843453559027	24.579456321785425	19.94693832600117
32	30.068765355277094	26.799000584186004	25.43144334732691	17.70079071320999
33	27.73233857046194	28.204582030547172	24.61512384607401	19.447955552916874
34	21.301579115055638	27.778792461689132	27.5107648395256	23.408863583729627
35	21.081229047595524	25.940306069960013	30.058568159641858	22.91989672280261
36	29.05040541783802	26.367591227511216	25.340982891824975	19.241020462825784
37	22.039357549482457	31.62454016312794	27.743351541748	18.592750745641606
38	21.244701423845758	32.44992383827882	24.1193588547235	22.18601588315192
39	21.73067710738644	31.212210892508413	26.25922902856076	20.797882971544386
40	24.323121483950295	25.82251713015565	25.790112708470335	24.064248677423727
41	18.473058329318658	25.176377493837492	27.864630188503092	28.485933988340754
42	22.22811897090808	26.893222671855604	24.115053372121956	26.76360498511436
43	22.803648692560895	27.724951268735083	25.000215274130078	24.471184764573945
44	21.113678790150324	30.13765307690181	26.88778416751681	21.860883965431054
45	19.536376569291757	34.54710175305655	22.757421405681153	23.159100271970537
46	21.852998134139803	32.581490322408136	25.134501010428785	20.43101053302328
47	21.760996769075213	28.666175086302264	26.641102674883037	22.93172546973948
48	23.088173111218772	24.65690968774374	29.504973738822176	22.74994346221531
49	21.27357081771085	25.079526795737483	30.764576664556227	22.882325721995443
50	19.5654725675043	31.925697340888625	26.38082492140228	22.128005170204794
51	18.881852572117968	32.2895786020234	24.853375656982653	23.975193168875983
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1130.0
1	1774.0
2	2418.0
3	6515.5
4	10613.0
5	8316.0
6	6019.0
7	6601.0
8	7183.0
9	7573.5
10	7964.0
11	8074.0
12	8184.0
13	8196.5
14	8209.0
15	7994.5
16	7780.0
17	7763.0
18	7746.0
19	7688.5
20	7631.0
21	7572.5
22	7514.0
23	8562.0
24	9610.0
25	10292.0
26	14879.5
27	18785.0
28	21573.0
29	24361.0
30	29438.5
31	34516.0
32	39741.0
33	44966.0
34	54234.5
35	63503.0
36	65176.0
37	66849.0
38	79418.5
39	91988.0
40	117700.0
41	143412.0
42	171761.5
43	200111.0
44	201210.0
45	202309.0
46	198233.0
47	194157.0
48	182316.0
49	170475.0
50	164299.5
51	158124.0
52	149968.0
53	141812.0
54	130253.0
55	118694.0
56	110142.5
57	101591.0
58	95250.5
59	88910.0
60	85274.0
61	81638.0
62	69537.5
63	57437.0
64	47445.5
65	37454.0
66	30514.5
67	23575.0
68	19073.0
69	14571.0
70	12204.0
71	9837.0
72	8302.0
73	6767.0
74	4996.0
75	2770.0
76	2315.0
77	1688.0
78	1061.0
79	873.5
80	686.0
81	443.0
82	200.0
83	169.0
84	138.0
85	81.5
86	25.0
87	16.5
88	8.0
89	7.0
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2206489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.366003715591777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.55248727525512	13.346883097625767
2	7.51236844826889	2.4589489987452624
3	2.3171398547513955	1.137669584172214
4	1.107180530319	0.7248048269212654
5	0.6320427131125947	0.5172006695606718
6	0.4257405913822577	0.41806032602641635
7	0.305002888378638	0.349417488312971
8	0.24423223416899725	0.31976845215016725
9	0.1916873130075383	0.2823439749221859
>10	2.5390370508048687	10.117485265607549
>50	1.2900560975735915	15.426309571866812
>100	1.8717998166703216	53.28435520928074
>500	0.010102667676126196	0.9815702282233133
>1k	5.612593153403443E-4	0.10195984964700336
>5k	5.612593153403443E-4	0.5332224569377068
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6360	0.2882407299560524	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5250	0.23793456482221303	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029911773863363925	0.0
2	0.0	0.0	0.0	0.09345163288826729	0.0
3	0.0	0.0	0.0	0.13061474587002248	0.0
4	0.0	0.0	0.0	0.21169378138753467	0.0
5	0.0	0.0	0.0	0.36474235765508006	0.0
6	0.0	0.0	0.0	0.4963541626538813	0.0
7	0.0	0.0	0.0	0.582101247728858	0.0
8	0.0	0.0	0.0	0.7804706934863487	0.0
9	0.0	0.0	0.0	0.8486786020687164	0.0
10	0.0	0.0	0.0	1.0146436261408962	0.0
11	0.0	0.0	0.0	1.1585373867714728	0.0
12	0.0	0.0	0.0	1.3259526786673308	0.0
13	0.0	0.0	0.0	1.3808362516196546	0.0
14	0.0	0.0	0.0	1.402907515061258	0.0
15	0.0	0.0	0.0	1.4403425532599528	0.0
16	4.532086948994534E-5	0.0	0.0	1.5201526044317466	0.0
17	4.532086948994534E-5	0.0	0.0	1.6072139947219315	0.0
18	4.532086948994534E-5	0.0	0.0	1.729625663214274	0.0
19	4.532086948994534E-5	0.0	0.0	1.8028188674405357	0.0
20	4.532086948994534E-5	0.0	0.0	1.8682168821145266	0.0
21	4.532086948994534E-5	0.0	0.0	1.9507008645862272	0.0
22	4.532086948994534E-5	0.0	0.0	2.0432460800846957	0.0
23	4.532086948994534E-5	0.0	0.0	2.1453993199150325	0.0
24	4.532086948994534E-5	0.0	0.0	2.224574878913967	0.0
25	4.532086948994534E-5	0.0	0.0	2.2885679466337696	0.0
26	4.532086948994534E-5	0.0	0.0	2.3550083413060294	0.0
27	4.532086948994534E-5	0.0	0.0	2.4247571594510555	0.0
28	4.532086948994534E-5	0.0	0.0	2.4942793732486317	0.0
29	4.532086948994534E-5	0.0	0.0	2.5723219105103174	0.0
30	9.064173897989068E-5	0.0	0.0	2.6651843720952155	0.0
31	9.064173897989068E-5	0.0	0.0	2.76099269019696	0.0
32	9.064173897989068E-5	0.0	0.0	2.8492777439633734	0.0
33	9.064173897989068E-5	0.0	0.0	2.9362938133840686	0.0
34	9.064173897989068E-5	0.0	0.0	3.022901994979354	0.0
35	9.064173897989068E-5	0.0	0.0	3.135660318270338	0.0
36	9.064173897989068E-5	0.0	0.0	3.2420283989632397	0.0
37	9.064173897989068E-5	0.0	0.0	3.347263457918893	0.0
38	1.35962608469836E-4	0.0	0.0	3.453087688177915	0.0
39	1.35962608469836E-4	0.0	0.0	3.557960180177649	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	25	3.8919767E-5	45.0	10
GTCGATA	20	7.034701E-4	45.0	44
ACGTTGA	25	3.8919767E-5	45.0	14
CGTCTAG	35	1.2126111E-7	45.0	18
CATTACG	20	7.034701E-4	45.0	39
CTACGAT	20	7.034701E-4	45.0	41
TTAACGC	25	3.8919767E-5	45.0	28
ACTCGAC	25	3.8919767E-5	45.0	35
TCCAACG	25	3.8919767E-5	45.0	27
CGACTCA	20	7.034701E-4	45.0	20
CCCGGTA	20	7.034701E-4	45.0	19
CGATCGC	25	3.8919767E-5	45.0	34
TAATGCG	90	0.0	45.0	1
CGATACA	45	3.8562575E-10	45.0	10
CCGTTCG	20	7.034701E-4	45.0	36
TACCGAA	25	3.8919767E-5	45.0	30
CGTTTAC	95	0.0	42.63158	33
CGTTTTT	5720	0.0	41.892483	1
CACGACG	265	0.0	41.603775	26
CGGTCTA	260	0.0	41.53846	31
>>END_MODULE