FastQCFastQC Report
Thu 26 May 2016
SRR1546278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546278_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1712045
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC89520.5228834522457062No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40780.23819467362131255No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG34940.20408342070447916No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25240.14742603144193056No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22790.13311565992716312No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA19880.11611844314839855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG207.034255E-445.00000442
GCACGAC351.2123928E-745.00000412
GTTAGCG700.045.0000041
CAATTCG207.034255E-445.0000041
CACTCGA207.034255E-445.00000438
TACGCAC351.2123928E-745.0000041
CGTAGGT207.034255E-445.0000043
AACGCGT351.2123928E-745.00000433
CCGAACG207.034255E-445.0000041
ATACCCG406.8175723E-945.00000419
TGTCCGA207.034255E-445.00000445
GACGTAT253.8916092E-545.024
TCTTACG502.1827873E-1145.01
CGTAATC253.8916092E-545.031
TGTACGA253.8916092E-545.021
CTTAGTA253.8916092E-545.030
CTATGCG600.044.9999961
ACGCATG650.044.9999961
AATCTCG302.1661272E-644.99999618
TCGAATA302.1661272E-644.99999639