##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546278_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1712045
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234306925343667	31.0	31.0	33.0	30.0	34.0
2	31.46770324378156	31.0	31.0	34.0	30.0	34.0
3	31.549515929779883	31.0	31.0	34.0	30.0	34.0
4	35.32710296750378	37.0	35.0	37.0	33.0	37.0
5	35.03248921611289	37.0	35.0	37.0	32.0	37.0
6	35.181345700609505	37.0	35.0	37.0	32.0	37.0
7	35.70089629653426	37.0	35.0	37.0	35.0	37.0
8	35.77757418759437	37.0	35.0	37.0	35.0	37.0
9	37.47687882035811	39.0	37.0	39.0	35.0	39.0
10	36.834812169072656	39.0	37.0	39.0	32.0	39.0
11	36.507038658446476	39.0	35.0	39.0	32.0	39.0
12	35.497442532176436	37.0	35.0	39.0	31.0	39.0
13	34.94180468387221	37.0	34.0	39.0	29.0	39.0
14	35.880764232248566	38.0	34.0	40.0	29.0	41.0
15	36.20174878580878	38.0	35.0	40.0	30.0	41.0
16	36.373373363433785	38.0	35.0	40.0	31.0	41.0
17	36.326484993093054	37.0	35.0	40.0	31.0	41.0
18	36.296571059756026	37.0	35.0	40.0	31.0	41.0
19	36.237558008113105	37.0	35.0	40.0	31.0	41.0
20	36.16882032890491	37.0	35.0	40.0	31.0	41.0
21	35.985293026760395	37.0	34.0	40.0	30.0	41.0
22	35.857848362630655	37.0	34.0	40.0	30.0	41.0
23	35.85674033100766	36.0	34.0	40.0	30.0	41.0
24	35.74349213951736	36.0	34.0	40.0	30.0	41.0
25	35.59235066835276	36.0	34.0	40.0	30.0	41.0
26	35.50607665102261	36.0	34.0	40.0	30.0	41.0
27	35.440121608953035	36.0	34.0	40.0	29.0	41.0
28	35.45212304583116	36.0	34.0	40.0	29.0	41.0
29	35.50650187349048	36.0	34.0	40.0	30.0	41.0
30	35.42168225718366	36.0	34.0	40.0	29.0	41.0
31	35.31322716400562	36.0	34.0	40.0	29.0	41.0
32	35.136266862144396	36.0	34.0	40.0	29.0	41.0
33	34.96775201586407	36.0	34.0	40.0	27.0	41.0
34	34.84511037969212	36.0	34.0	40.0	27.0	41.0
35	34.71157300187787	35.0	33.0	40.0	27.0	41.0
36	34.52733602212559	35.0	33.0	40.0	25.0	41.0
37	34.43452946622314	35.0	33.0	40.0	25.0	41.0
38	34.413221030989256	35.0	33.0	40.0	25.0	41.0
39	34.36133512845749	35.0	33.0	40.0	25.0	41.0
40	34.190500833798176	35.0	33.0	40.0	24.0	41.0
41	34.177309007648745	35.0	33.0	40.0	24.0	41.0
42	34.1262922411502	35.0	33.0	40.0	24.0	41.0
43	34.058574979045524	35.0	33.0	40.0	24.0	41.0
44	33.82684917744569	35.0	33.0	39.0	23.0	41.0
45	33.80912183967127	35.0	33.0	39.0	23.0	41.0
46	33.75559462514128	35.0	33.0	39.0	23.0	41.0
47	33.751856405643544	35.0	33.0	39.0	23.0	41.0
48	33.68197097623018	35.0	33.0	39.0	23.0	41.0
49	33.59610816304478	35.0	33.0	39.0	24.0	41.0
50	33.510952691079964	35.0	32.0	39.0	24.0	41.0
51	33.429750386234005	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	5.0
13	11.0
14	25.0
15	54.0
16	171.0
17	528.0
18	1157.0
19	2178.0
20	3711.0
21	5781.0
22	8530.0
23	11945.0
24	16824.0
25	22534.0
26	28817.0
27	33801.0
28	38440.0
29	44839.0
30	53782.0
31	65369.0
32	81369.0
33	105335.0
34	182320.0
35	258799.0
36	119753.0
37	143609.0
38	196997.0
39	285180.0
40	178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.65749439997197	25.454120656875258	28.77377639022339	13.114608552929392
2	29.956513993499	26.659462806176236	30.53646370276482	12.847559497559935
3	30.349435908518757	25.23613573241358	30.98201273915113	13.432415619916533
4	27.49986127701083	28.57781191499055	28.99450656962872	14.927820238369902
5	23.684132134377307	33.411972232038295	28.91039663092968	13.993499002654719
6	22.072842711494147	41.91957571208701	25.445359204927442	10.562222371491403
7	85.85831564006787	6.120341462987247	6.09224640707458	1.9290964898703011
8	87.5103750193482	4.903375787435494	5.526431840284572	2.0598173529317276
9	82.68211407994532	6.628622495319925	7.636423108037464	3.052840316697283
10	52.94673913360922	23.329176511131426	12.43641376248872	11.287670592770633
11	48.8196279887503	20.663008273731123	17.190961686170635	13.32640205134795
12	42.88479566833816	21.525251964755597	22.284052113116186	13.30590025379006
13	22.75717051829829	41.28705729113429	21.768294641788035	14.187477548779384
14	16.09770771212205	44.23721339100315	25.409495661621044	14.255583235253747
15	15.946485051502734	22.67498809902777	46.240665403070594	15.137861446398897
16	17.2730856957615	18.40115183888274	44.78930168307492	19.53646078228084
17	17.27962757988254	19.274493369041117	27.14566497960042	36.30021407147592
18	21.97967927244903	23.951882105902587	32.94451956578244	21.12391905586594
19	30.982888884345915	24.236629294206637	23.84838015355905	20.932101667888404
20	32.50434421992413	23.63541846154745	23.02094863160723	20.839288686921197
21	22.995014733841693	28.679094299507312	26.06713024482417	22.25876072182682
22	22.930004760388893	25.62590352473212	23.98202150060308	27.462070214275908
23	21.739206621321287	30.750418359330506	23.32485419483717	24.185520824511038
24	22.05958371421312	24.33154502364132	36.39997780432173	17.20889345782383
25	19.2587811652147	25.032285950427706	33.88783589216405	21.821096992193546
26	17.86214731505305	34.76310494175095	26.12244421145472	21.25230353174128
27	20.170264216185906	33.13855652158676	27.0150609358983	19.67611832632904
28	16.394195246036173	29.634326200537952	35.88059893285515	18.09087962057072
29	17.52500664410106	25.623099860108816	35.324188324489135	21.527705171300987
30	19.086823068318882	31.227742261447567	29.90826759810636	19.777167072127195
31	30.170176601666427	27.071951963879453	23.3631125350093	19.394758899444817
32	31.69799859232672	26.74526662558519	24.264023433963477	17.292711348124612
33	29.21272513280901	27.68046400649516	23.368486225537296	19.738324635158538
34	20.149470370229754	28.615836616444078	27.43158036149751	23.803112651828663
35	22.368395690533834	25.970520634679577	29.809496829814634	21.851586844971948
36	31.248127239646156	25.974317263856967	23.87390518356702	18.90365031292986
37	22.00345201206744	33.720433750281096	26.072270296633558	18.203843941017904
38	21.956373810267838	34.18741913909973	22.04282013615296	21.81338691447947
39	22.873989877602515	32.131223186306435	24.64643160664585	20.348355329445194
40	24.149657281204643	26.720851379490608	25.04186513789065	24.087626201414096
41	18.119617182959562	25.656685425908783	25.367382282591873	30.85631510853979
42	22.3646574710361	27.04724466938661	22.802087561950763	27.78601029762652
43	23.36141865429939	26.254975774585365	24.756592262469738	25.62701330864551
44	21.101723377598137	30.326831362493394	27.562534863277545	21.00891039663093
45	18.590749659033495	37.031912128477934	21.758072947848916	22.619265264639658
46	21.636522404492872	33.770666074781914	24.468924590183086	20.123886930542128
47	23.461649664582414	27.280124062159583	25.98851081601243	23.269715457245574
48	23.836990266026888	24.40683510071289	29.853187270194415	21.902987363065808
49	21.899833240364593	24.50946090786165	30.833243285077206	22.757462566696553
50	20.101866481313284	33.43165629408105	25.117564082719788	21.348913141885873
51	20.647880166701228	33.47119964720554	22.622653026059478	23.25826716003376
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1214.0
1	1780.0
2	2346.0
3	5232.0
4	8118.0
5	6499.0
6	4880.0
7	5412.0
8	5944.0
9	6171.0
10	6398.0
11	6484.5
12	6571.0
13	6367.0
14	6163.0
15	6101.5
16	6040.0
17	5819.0
18	5598.0
19	5687.0
20	5776.0
21	6307.5
22	6839.0
23	6973.5
24	7108.0
25	9128.5
26	11393.0
27	11637.0
28	16669.0
29	21701.0
30	25030.5
31	28360.0
32	30619.5
33	32879.0
34	34710.5
35	36542.0
36	39244.0
37	41946.0
38	54029.0
39	66112.0
40	89686.0
41	113260.0
42	138506.0
43	163752.0
44	162757.0
45	161762.0
46	158930.0
47	156098.0
48	146545.5
49	136993.0
50	129012.0
51	121031.0
52	113217.0
53	105403.0
54	96098.0
55	86793.0
56	81564.5
57	76336.0
58	71143.0
59	65950.0
60	61475.5
61	57001.0
62	51283.5
63	45566.0
64	38994.0
65	32422.0
66	27874.0
67	23326.0
68	19186.0
69	15046.0
70	13042.5
71	11039.0
72	9137.5
73	7236.0
74	6078.0
75	3714.5
76	2509.0
77	1834.5
78	1160.0
79	970.0
80	780.0
81	499.5
82	219.0
83	159.5
84	100.0
85	56.0
86	12.0
87	6.5
88	1.0
89	2.5
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1712045.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.00508345203112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.60518542428655	13.877029882584663
2	7.30218905403353	2.483486684926967
3	2.3026800847575846	1.1747180101389845
4	1.0839976233043636	0.7373388018437638
5	0.6492032248830952	0.551987750823238
6	0.4103780205089969	0.41871074913808987
7	0.30060639973815845	0.35782858396334066
8	0.23907653597695352	0.32524131565684916
9	0.18637680605715448	0.28524178264724376
>10	2.7058084450228654	11.580814065677886
>50	1.436709550569009	17.88141600245674
>100	1.7666515476613953	47.87861098168907
>500	0.007656882200266087	0.7849641488829548
>1k	0.0031323609001088536	1.1327915109416993
>5k	3.480401000120949E-4	0.5298197286285314
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8952	0.5228834522457062	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4078	0.23819467362131255	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3494	0.20408342070447916	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2524	0.14742603144193056	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2279	0.13311565992716312	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1988	0.11611844314839855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033936023877877045	0.0
2	0.0	0.0	0.0	0.10887564287153667	0.0
3	0.0	0.0	0.0	0.1609186674415684	0.0
4	0.0	0.0	0.0	0.2533227806512095	0.0
5	0.0	0.0	0.0	0.40781638333104564	0.0
6	5.840967965211195E-5	0.0	0.0	0.5676252668592239	0.0
7	5.840967965211195E-5	0.0	0.0	0.6606134768653862	0.0
8	5.840967965211195E-5	0.0	0.0	0.8916237598894888	0.0
9	5.840967965211195E-5	0.0	0.0	0.9814578471944371	0.0
10	5.840967965211195E-5	0.0	0.0	1.1389303435365308	0.0
11	5.840967965211195E-5	0.0	0.0	1.3234465215575526	0.0
12	5.840967965211195E-5	0.0	0.0	1.4853581535532068	0.0
13	5.840967965211195E-5	0.0	0.0	1.547097184945489	0.0
14	5.840967965211195E-5	0.0	0.0	1.5764188441308493	0.0
15	5.840967965211195E-5	0.0	0.0	1.6170719811687193	0.0
16	5.840967965211195E-5	0.0	0.0	1.7142072784301814	0.0
17	5.840967965211195E-5	0.0	0.0	1.8189358340464181	0.0
18	5.840967965211195E-5	0.0	0.0	1.974247172241384	0.0
19	5.840967965211195E-5	0.0	0.0	2.0409510264040955	0.0
20	1.168193593042239E-4	0.0	0.0	2.1098744483935876	0.0
21	1.168193593042239E-4	0.0	0.0	2.201227187369491	0.0
22	1.168193593042239E-4	0.0	0.0	2.288374429410442	0.0
23	1.168193593042239E-4	0.0	0.0	2.3940375399011122	0.0
24	1.168193593042239E-4	0.0	0.0	2.4745260784617225	0.0
25	1.168193593042239E-4	0.0	0.0	2.5365571582522657	0.0
26	1.168193593042239E-4	0.0	0.0	2.599814841315503	0.0
27	1.168193593042239E-4	0.0	0.0	2.66196274046535	0.0
28	1.168193593042239E-4	0.0	0.0	2.7296011495024954	0.0
29	1.168193593042239E-4	0.0	0.0	2.7993423070071173	0.0
30	1.168193593042239E-4	0.0	0.0	2.892505746052236	0.0
31	1.168193593042239E-4	0.0	0.0	2.9815220978420545	0.0
32	1.168193593042239E-4	0.0	0.0	3.058038778186321	0.0
33	1.168193593042239E-4	0.0	0.0	3.1344386391712833	0.0
34	1.7522903895633585E-4	0.0	0.0	3.2200672295412796	0.0
35	1.7522903895633585E-4	0.0	0.0	3.324737375477864	0.0
36	2.336387186084478E-4	0.0	0.0	3.412877582072901	0.0
37	2.336387186084478E-4	0.0	0.0	3.506566708234889	0.0
38	2.336387186084478E-4	0.0	0.0	3.5936555405961874	0.0
39	2.336387186084478E-4	0.0	0.0	3.683664856940092	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	20	7.034255E-4	45.000004	42
GCACGAC	35	1.2123928E-7	45.000004	12
GTTAGCG	70	0.0	45.000004	1
CAATTCG	20	7.034255E-4	45.000004	1
CACTCGA	20	7.034255E-4	45.000004	38
TACGCAC	35	1.2123928E-7	45.000004	1
CGTAGGT	20	7.034255E-4	45.000004	3
AACGCGT	35	1.2123928E-7	45.000004	33
CCGAACG	20	7.034255E-4	45.000004	1
ATACCCG	40	6.8175723E-9	45.000004	19
TGTCCGA	20	7.034255E-4	45.000004	45
GACGTAT	25	3.8916092E-5	45.0	24
TCTTACG	50	2.1827873E-11	45.0	1
CGTAATC	25	3.8916092E-5	45.0	31
TGTACGA	25	3.8916092E-5	45.0	21
CTTAGTA	25	3.8916092E-5	45.0	30
CTATGCG	60	0.0	44.999996	1
ACGCATG	65	0.0	44.999996	1
AATCTCG	30	2.1661272E-6	44.999996	18
TCGAATA	30	2.1661272E-6	44.999996	39
>>END_MODULE