FastQCFastQC Report
Thu 26 May 2016
SRR1546277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546277_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2763236
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC107980.39077371603438865No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63130.22846401827422633No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG253.892232E-545.00000426
CACGCTA253.892232E-545.00000417
ATCGTCC302.1666128E-645.00000435
CCGTACT302.1666128E-645.00000425
CACTCCG253.892232E-545.00000443
TGCGCCC253.892232E-545.00000414
TACGATG253.892232E-545.0000041
CGCTAAC253.892232E-545.00000419
TCGAGTA302.1666128E-645.00000420
ACGAACT253.892232E-545.00000418
ACGCGCT253.892232E-545.00000427
GTCGACC207.03501E-445.039
CGCGACA207.03501E-445.042
TCTACGA351.2127566E-745.023
AACGCGT406.8212103E-945.014
CGTCGGA406.8212103E-945.015
TTCGGAC207.03501E-445.014
CCAACGT453.8562575E-1045.045
CGACTCG207.03501E-445.038
CGTCCTA207.03501E-445.011