##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546276_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2688033
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.167001297975137	31.0	31.0	33.0	30.0	34.0
2	31.40616726059539	31.0	31.0	34.0	30.0	34.0
3	31.490005517045365	31.0	31.0	34.0	30.0	34.0
4	35.2779441323823	37.0	35.0	37.0	33.0	37.0
5	34.994834884839584	37.0	35.0	37.0	32.0	37.0
6	35.13444961427185	37.0	35.0	37.0	32.0	37.0
7	35.69095059472856	37.0	35.0	37.0	35.0	37.0
8	35.752511594909734	37.0	35.0	37.0	35.0	37.0
9	37.457364548723916	39.0	37.0	39.0	35.0	39.0
10	36.73339836229689	39.0	37.0	39.0	32.0	39.0
11	36.43821857841775	38.0	35.0	39.0	32.0	39.0
12	35.75760639843335	37.0	35.0	39.0	31.0	39.0
13	35.349046310071344	37.0	35.0	39.0	30.0	39.0
14	36.28898901166764	38.0	35.0	40.0	30.0	41.0
15	36.53139116967686	38.0	35.0	40.0	31.0	41.0
16	36.656852427034934	38.0	35.0	40.0	31.0	41.0
17	36.59756111625118	38.0	35.0	40.0	31.0	41.0
18	36.556867791429646	38.0	35.0	40.0	31.0	41.0
19	36.51066448961006	38.0	35.0	40.0	31.0	41.0
20	36.44316755039838	38.0	35.0	40.0	31.0	41.0
21	36.29988061902514	38.0	35.0	40.0	30.0	41.0
22	36.200171277659166	38.0	35.0	40.0	30.0	41.0
23	36.15248994338983	38.0	34.0	40.0	30.0	41.0
24	36.04955891538534	38.0	34.0	40.0	30.0	41.0
25	35.91614277056866	37.0	34.0	40.0	30.0	41.0
26	35.8850653991227	37.0	34.0	40.0	30.0	41.0
27	35.79508659305894	37.0	34.0	40.0	30.0	41.0
28	35.766386052552186	37.0	34.0	40.0	29.0	41.0
29	35.763827676222725	37.0	34.0	40.0	30.0	41.0
30	35.652339833625554	37.0	34.0	40.0	29.0	41.0
31	35.55611891669485	37.0	34.0	40.0	29.0	41.0
32	35.406709292631454	37.0	34.0	40.0	29.0	41.0
33	35.24187872693527	37.0	34.0	40.0	27.0	41.0
34	35.09741658677554	37.0	34.0	40.0	27.0	41.0
35	34.95240311409867	37.0	34.0	40.0	26.0	41.0
36	34.80800384519089	37.0	33.0	40.0	25.0	41.0
37	34.72567970705717	37.0	33.0	40.0	25.0	41.0
38	34.67526365933752	37.0	33.0	40.0	25.0	41.0
39	34.61148096024119	36.0	33.0	40.0	25.0	41.0
40	34.47373525548235	36.0	33.0	40.0	24.0	41.0
41	34.38458084405958	36.0	33.0	40.0	24.0	41.0
42	34.33032406968218	36.0	33.0	40.0	24.0	41.0
43	34.284032599302165	36.0	33.0	40.0	24.0	41.0
44	34.06965241870171	36.0	33.0	40.0	23.0	41.0
45	34.07649310852955	35.0	33.0	40.0	23.0	41.0
46	34.01889448529836	35.0	33.0	40.0	23.0	41.0
47	33.98809947645732	35.0	33.0	40.0	23.0	41.0
48	33.874591941393575	35.0	33.0	40.0	23.0	41.0
49	33.797012164657204	35.0	33.0	39.0	24.0	41.0
50	33.71819021567072	35.0	33.0	39.0	24.0	41.0
51	33.64256763216821	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	10.0
13	10.0
14	25.0
15	131.0
16	318.0
17	845.0
18	1760.0
19	3441.0
20	5844.0
21	9010.0
22	13447.0
23	18937.0
24	25931.0
25	34873.0
26	44666.0
27	51994.0
28	59189.0
29	68612.0
30	82173.0
31	99614.0
32	122608.0
33	156840.0
34	247196.0
35	331600.0
36	204050.0
37	253964.0
38	350326.0
39	500441.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.11783523490969	24.361084852752924	27.47615077642276	13.044929135914627
2	30.339806096130516	26.274603027567	29.864179494820192	13.521411381482295
3	29.35975860415404	25.704595144479253	30.631841201354298	14.303805050012407
4	26.78642710115538	28.267547310617097	29.426684865848003	15.519340722379523
5	23.83181307669958	31.83707194070906	29.12683735653543	15.204277626055932
6	22.2815716920142	40.77903805496435	26.18293748625854	10.756452766762909
7	86.13711215598916	5.031002223559011	6.727856391644002	2.104029228807831
8	86.96541299902196	4.373086193510273	6.208889548603012	2.4526112588647537
9	82.64879932649636	5.835419431234661	8.169616965267911	3.346164277001064
10	46.671897257213736	25.602885083628063	15.134300806574918	12.590916852583284
11	40.38001021564839	23.544279404307908	21.633030546872007	14.44267983317169
12	36.401041207455414	22.819362708716746	25.52148727340773	15.25810881042011
13	23.46968954622209	35.18963494867808	25.24001751466593	16.1006579904339
14	18.328718434632314	37.60102647549342	28.02666485121276	16.043590238661505
15	16.71229482673762	24.829419876913715	42.105956288483064	16.352329007865603
16	19.3626343128972	21.7604099354435	39.76033776371049	19.116617987948807
17	19.777807787329994	21.1702014074976	28.29801568656337	30.753975118609034
18	23.193502460721277	23.70785626515746	32.63382555199285	20.464815722128414
19	28.54808702125309	24.819672972764845	25.937107170931313	20.69513283505076
20	30.143193926562656	24.68031456459054	25.358952066436686	19.81753944241012
21	23.942302791669594	27.25085592327178	28.74350872924551	20.06333255581312
22	23.912429646511036	24.03549361187158	27.096095918465284	24.955980823152096
23	21.065366385010897	28.743731940790905	26.367161415057033	23.823740259141164
24	20.761687077502398	25.958423873516434	34.21390287991256	19.06598616906861
25	20.36079914197482	25.8942877561399	32.58055239649216	21.164360705393126
26	19.608687839769825	31.509322988222245	26.88493779652259	21.997051375485345
27	19.696037957867333	31.654596502349484	28.278335868644472	20.37102967113871
28	18.119643620446624	28.78755580753659	33.35100424734369	19.741796324673096
29	18.44129145735934	25.993840105385612	33.46398649123727	22.100881946017775
30	21.08742712608067	28.255047464075034	30.850439708143462	19.807085701700835
31	26.87280996922285	26.86536958437638	25.8412378121846	20.42058263421617
32	28.148054729982853	26.23118838198787	26.422592282163205	19.198164605866076
33	27.123178919306422	27.14739737198167	25.423162587661686	20.306261121050227
34	21.46216210887292	27.43083883270778	28.178671913626058	22.928327144793236
35	20.886536735226095	26.66261909731019	29.19123388738159	23.25961028008213
36	28.089610507013862	26.663325933870603	25.71940151032372	19.52766204879181
37	21.948354056665227	30.881354507180532	27.4956445847205	19.674646851433746
38	22.024766809038432	31.67959619543361	24.360675631586368	21.93496136394159
39	21.330132479772384	30.263095728363453	26.431855561297052	21.97491623056711
40	23.648035570991873	26.314929913434842	26.41105968565118	23.625974829922104
41	19.87635568462143	25.4595460695609	27.03962339748061	27.624474848337055
42	22.389717685757578	26.764998792797556	25.508913023017204	25.336370498427662
43	22.220523334348947	26.687916405788176	27.175633632474007	23.915926627388874
44	21.201823043095082	29.146777587923957	27.819710546708322	21.831688822272643
45	19.66359787993674	32.295660060720984	25.03477449867617	23.005967560666107
46	22.239607921480133	30.17105816781267	26.49353635167425	21.095797559032945
47	22.059773819741054	27.858214538288777	27.016707012153496	23.065304629816673
48	22.49890533337946	26.641451202421994	28.933312946678853	21.926330517519688
49	22.407686215161792	25.259771736433294	29.588066813167845	22.744475235237065
50	21.02637876841542	30.784927119570334	26.837319333505206	21.351374778509044
51	20.234535811130293	31.448535044026617	25.095413635174868	23.221515509668222
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1419.0
1	2292.0
2	3165.0
3	8714.5
4	14264.0
5	11314.5
6	8365.0
7	8758.0
8	9151.0
9	9746.0
10	10341.0
11	10348.0
12	10355.0
13	10294.5
14	10234.0
15	10049.5
16	9865.0
17	9874.0
18	9883.0
19	10444.0
20	11005.0
21	11730.0
22	12455.0
23	12510.0
24	12565.0
25	14036.0
26	21258.5
27	27010.0
28	29458.0
29	31906.0
30	39096.5
31	46287.0
32	52194.5
33	58102.0
34	66633.0
35	75164.0
36	84116.5
37	93069.0
38	103903.0
39	114737.0
40	140614.0
41	166491.0
42	193201.0
43	219911.0
44	223793.0
45	227675.0
46	227511.0
47	227347.0
48	225240.5
49	223134.0
50	208734.5
51	194335.0
52	179359.0
53	164383.0
54	151703.0
55	139023.0
56	133350.0
57	127677.0
58	117436.5
59	107196.0
60	97794.5
61	88393.0
62	76818.5
63	65244.0
64	58232.0
65	51220.0
66	42489.0
67	33758.0
68	29489.0
69	25220.0
70	20748.0
71	16276.0
72	14229.5
73	12183.0
74	9394.0
75	4827.5
76	3050.0
77	2507.5
78	1965.0
79	1628.5
80	1292.0
81	905.0
82	518.0
83	371.0
84	224.0
85	133.5
86	43.0
87	28.0
88	13.0
89	8.5
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2688033.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.98908835507173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.18906437772347	14.605172525564742
2	7.286396787447111	2.6215127119899373
3	2.3495540367948147	1.267990054887521
4	1.2832397769229886	0.923372549112407
5	0.7598271661062407	0.6834299012834465
6	0.5150554364013901	0.5559226651910779
7	0.38642757855120574	0.48660358973758433
8	0.29500174509711147	0.42454499659618294
9	0.23093836739633464	0.3738933626101812
>10	2.7393390063496876	11.864731846038442
>50	1.2939782742530836	17.069544115302993
>100	1.6636222057443177	47.64052058731734
>500	0.005666430909213067	0.6507914893626543
>1k	0.0014690746801663507	0.33921345468867903
>5k	4.1973562290467166E-4	0.49275615031682124
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7003	0.26052507539899994	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6049	0.22503443968135808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04363785712452191	0.0
2	0.0	0.0	0.0	0.12719337895033284	0.0
3	0.0	0.0	0.0	0.182847457601897	0.0
4	0.0	0.0	0.0	0.29352318219307577	0.0
5	0.0	0.0	0.0	0.4824717553690747	0.0
6	0.0	0.0	0.0	0.6730200112870638	0.0
7	0.0	0.0	0.0	0.788048361013425	0.0
8	0.0	0.0	0.0	1.0820551682215211	0.0
9	0.0	0.0	0.0	1.195893056372448	0.0
10	0.0	0.0	0.0	1.4040006205280962	0.0
11	0.0	0.0	0.0	1.5952185110822672	0.0
12	0.0	0.0	0.0	1.7800376706684777	0.0
13	7.440384846465799E-5	0.0	0.0	1.8503493074675794	0.0
14	7.440384846465799E-5	0.0	0.0	1.8769486832936946	0.0
15	7.440384846465799E-5	0.0	0.0	1.9252367809472577	0.0
16	7.440384846465799E-5	0.0	0.0	2.0225942166632627	0.0
17	7.440384846465799E-5	0.0	0.0	2.135613662481078	0.0
18	7.440384846465799E-5	0.0	0.0	2.3013110330118716	0.0
19	7.440384846465799E-5	0.0	0.0	2.3743384102799334	0.0
20	1.1160577269698697E-4	0.0	0.0	2.4570382878484005	0.0
21	1.1160577269698697E-4	0.0	0.0	2.558153117911871	0.0
22	1.1160577269698697E-4	0.0	0.0	2.663583371186291	0.0
23	1.4880769692931597E-4	0.0	0.0	2.785903298062189	0.0
24	1.4880769692931597E-4	0.0	0.0	2.8737370411747176	0.0
25	1.4880769692931597E-4	0.0	0.0	2.954688428304266	0.0
26	1.4880769692931597E-4	0.0	0.0	3.0344121519341467	0.0
27	1.4880769692931597E-4	0.0	0.0	3.115177529442533	0.0
28	1.8600962116164496E-4	0.0	0.0	3.200332734010334	0.0
29	1.8600962116164496E-4	0.0	0.0	3.303679679527744	0.0
30	1.8600962116164496E-4	0.0	0.0	3.428566539175672	0.0
31	2.2321154539397395E-4	0.0	0.0	3.5386470329791337	0.0
32	2.6041346962630294E-4	0.0	0.0	3.644412103571645	0.0
33	2.6041346962630294E-4	0.0	0.0	3.752186078072702	0.0
34	2.6041346962630294E-4	0.0	0.0	3.864982312345124	0.0
35	2.6041346962630294E-4	0.0	0.0	4.008656143730378	0.0
36	2.6041346962630294E-4	0.0	0.0	4.126288628153002	0.0
37	2.6041346962630294E-4	0.0	0.0	4.249947824301264	0.0
38	2.6041346962630294E-4	0.0	0.0	4.387780953582043	0.0
39	2.6041346962630294E-4	0.0	0.0	4.5793708633785375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAA	30	2.166591E-6	45.000004	26
TATCCGC	30	2.166591E-6	45.000004	10
TGTTACG	45	3.8562575E-10	45.0	1
CGAATGT	25	3.892205E-5	45.0	37
CGAATCG	20	7.034975E-4	45.0	33
GCGTAAT	20	7.034975E-4	45.0	37
CGGTCTA	280	0.0	42.589283	31
CGTTTTT	6960	0.0	42.510777	1
GTGCGTA	65	0.0	41.538464	27
ACGGGTC	240	0.0	41.250004	5
CGAAATC	85	0.0	39.705883	40
GTCGAAT	40	3.4605364E-7	39.375	38
CGACGGT	310	0.0	39.193546	28
TATTCCG	35	6.251412E-6	38.571426	1
GTCGTTA	35	6.251412E-6	38.571426	29
CGTAACG	35	6.251412E-6	38.571426	26
CACGACG	330	0.0	38.18182	26
GGGCGAT	3940	0.0	37.97589	7
ACAACGA	380	0.0	37.894737	13
AGGGCGA	1895	0.0	37.875988	6
>>END_MODULE