##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546275_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1474694
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276942199534275	31.0	31.0	33.0	30.0	34.0
2	31.499871159711777	31.0	31.0	34.0	30.0	34.0
3	31.559170241419576	31.0	31.0	34.0	30.0	34.0
4	35.31732006775643	37.0	35.0	37.0	33.0	37.0
5	35.047138592819934	37.0	35.0	37.0	32.0	37.0
6	35.181640394549646	37.0	35.0	37.0	32.0	37.0
7	35.73879462451193	37.0	35.0	37.0	35.0	37.0
8	35.83404014663381	37.0	35.0	37.0	35.0	37.0
9	37.591062281395324	39.0	37.0	39.0	35.0	39.0
10	36.919656552478	39.0	37.0	39.0	32.0	39.0
11	36.50083407133955	39.0	35.0	39.0	32.0	39.0
12	35.16041429611838	35.0	35.0	39.0	30.0	39.0
13	34.462547484427276	35.0	33.0	39.0	26.0	39.0
14	35.29808082219091	36.0	33.0	40.0	27.0	41.0
15	35.75381808022546	36.0	34.0	40.0	30.0	41.0
16	36.01458946737425	36.0	34.0	40.0	31.0	41.0
17	35.981594147667245	36.0	34.0	40.0	31.0	41.0
18	35.98309208554453	36.0	35.0	40.0	31.0	41.0
19	35.86700630774927	36.0	34.0	40.0	31.0	41.0
20	35.74806298798259	36.0	34.0	40.0	30.0	41.0
21	35.55692909851128	35.0	34.0	40.0	30.0	41.0
22	35.398873935880935	35.0	34.0	40.0	30.0	41.0
23	35.43685062799469	35.0	34.0	40.0	30.0	41.0
24	35.34959862859685	35.0	34.0	40.0	30.0	41.0
25	35.22380236171029	35.0	34.0	40.0	29.0	41.0
26	35.176307084724016	35.0	34.0	40.0	29.0	41.0
27	35.058517902697105	35.0	34.0	40.0	29.0	41.0
28	35.084757922660565	35.0	34.0	40.0	29.0	41.0
29	35.15919234770061	35.0	34.0	40.0	29.0	41.0
30	35.10131389969716	36.0	34.0	40.0	29.0	41.0
31	34.92584766738049	35.0	34.0	40.0	29.0	41.0
32	34.72416989558512	35.0	34.0	40.0	29.0	41.0
33	34.54189886172996	35.0	33.0	40.0	27.0	41.0
34	34.437140857696576	35.0	34.0	40.0	27.0	41.0
35	34.26992108193293	35.0	33.0	40.0	26.0	41.0
36	34.06424993930944	35.0	33.0	39.0	24.0	41.0
37	33.95265865325281	35.0	33.0	39.0	24.0	41.0
38	33.99460837299128	35.0	33.0	39.0	24.0	41.0
39	33.95750982915778	35.0	33.0	39.0	24.0	41.0
40	33.758542450162544	35.0	33.0	39.0	23.0	41.0
41	33.768166141585986	35.0	33.0	39.0	23.0	41.0
42	33.72947201249887	35.0	33.0	39.0	23.0	41.0
43	33.66028952447084	35.0	33.0	39.0	23.0	41.0
44	33.43594942408391	35.0	33.0	39.0	23.0	41.0
45	33.41939548136766	35.0	33.0	39.0	23.0	41.0
46	33.40470701040351	35.0	33.0	38.0	23.0	40.0
47	33.38003002656822	35.0	33.0	38.0	23.0	40.0
48	33.34338717049096	35.0	33.0	38.0	23.0	40.0
49	33.31859558660983	35.0	32.0	38.0	24.0	40.0
50	33.22290251401308	35.0	32.0	38.0	24.0	40.0
51	33.1203341167727	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	4.0
13	4.0
14	9.0
15	47.0
16	182.0
17	450.0
18	1013.0
19	1989.0
20	3337.0
21	5259.0
22	7910.0
23	11282.0
24	15238.0
25	20985.0
26	26515.0
27	30922.0
28	34988.0
29	40586.0
30	47962.0
31	59072.0
32	73701.0
33	96897.0
34	178019.0
35	266346.0
36	90269.0
37	106421.0
38	146238.0
39	208957.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.923440388311064	24.245572301779216	29.50110327973125	13.329884030178466
2	29.470385042591886	26.570325776059306	31.027386020421865	12.931903160926945
3	29.11180217726525	25.949315586826827	31.455474830710646	13.483407405197282
4	25.95860564971445	28.643908498983517	30.14679655576004	15.250689295541992
5	23.737941566182545	32.7084127283355	29.05748582417776	14.496159881304187
6	22.16378448681557	39.82860173025726	26.666481317480102	11.34113246544707
7	86.43942404322524	5.735834010309936	5.905971001441655	1.9187709450231711
8	88.96883014374507	4.104512529378977	5.320764850199431	1.6058924766765175
9	85.76925111243418	4.888403967195906	6.85905008089814	2.4832948394717818
10	61.91881163142998	18.017432769103287	10.600843293591755	9.462912305874982
11	57.55756787509816	17.166951245478725	15.256249771138961	10.019231108284158
12	49.503964890343354	20.25498171146014	18.513128825369872	11.727924572826634
13	21.673106420721858	46.98920589627407	19.2626402494348	12.075047433569269
14	13.566068621693722	49.06305986191033	25.256900753647876	12.113970762748068
15	11.636447968188655	21.705248682099473	54.67222352569414	11.986079824017729
16	13.719795428746572	15.486602644345199	51.47162733421307	19.321974592695163
17	14.249396824019083	15.57957108389944	28.004589426687843	42.16644266539363
18	21.97825447177516	21.17313829174052	35.104231793172005	21.744375443312308
19	31.053899995524496	23.325042347768417	23.815516981828093	21.805540674878994
20	35.040015081094786	21.236676897037622	22.959000307860478	20.76430771400711
21	22.78845645266069	28.454581085974446	26.619285085583854	22.13767737578101
22	23.232006097536168	24.2305861419386	23.55959948301139	28.977808277513844
23	19.387005032908522	32.22438010868696	22.078139600486608	26.310475257917915
24	20.08111513303777	22.961916167014987	40.408315216580526	16.548653483366717
25	15.875225640031084	24.21668495294617	38.33717367806474	21.570915728958006
26	15.763134589277506	36.297360672790425	26.8101721441872	21.12933259374487
27	17.233948195354426	36.420911728128004	27.837707348100693	18.507432728416877
28	14.44916708144198	29.236234771416985	39.54861144074635	16.765986706394685
29	14.4366899166878	24.74486232398043	39.279470859717335	21.53897689961443
30	17.606161006961443	31.918893004243593	31.22118893817972	19.253757050615246
31	32.104423019284	26.159799931375595	22.467847567020684	19.26792948231972
32	33.526277315836374	25.65020268611658	24.96341613921261	15.860103858834442
33	31.170195308314806	27.46257867733916	23.04139028164487	18.325835732701158
34	19.7595568979056	28.163402034591584	27.137426476272363	24.939614591230452
35	19.914639918518688	26.038079764344328	30.729629333271852	23.317650983865125
36	33.87150147759467	23.152125118838214	24.73408042617655	18.242292977390566
37	21.81211831064614	32.76239002803293	27.951018991058486	17.474472670262443
38	20.84771484796168	33.60575143046625	21.413459334614505	24.133074386957563
39	21.047552916062585	32.347253057244416	26.384931382374923	20.220262644318076
40	25.776262736540595	25.03400705502294	23.007349321282923	26.18238088715354
41	16.960060866864584	24.224008506171447	25.0784230491207	33.73750757784327
42	22.986802685845333	25.288229286889347	22.556408312504153	29.168559714761166
43	23.854440311006893	26.390017183225805	23.183114598689627	26.57242790707767
44	19.909350685633765	31.035116437715217	27.154853820521407	21.90067905612961
45	17.249002165873055	39.47639306866373	20.524529156557225	22.750075608905984
46	23.124661794243416	33.55597839280556	24.489826363977883	18.829533448973145
47	23.38837752103148	27.062495677069276	25.254323947883428	24.294802854015813
48	24.7286555719356	22.595602884395	30.036875446702844	22.638866096966556
49	22.284894357744726	21.740035559919548	32.346168086396226	23.628901995939497
50	19.96773567940196	33.79501103279731	24.87783906356166	21.359414224239064
51	18.45311637532939	36.1816078454242	21.48466054652694	23.880615232719464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1104.0
1	1448.0
2	1792.0
3	3924.0
4	6056.0
5	4805.5
6	3555.0
7	4028.5
8	4502.0
9	5053.5
10	5605.0
11	5862.5
12	6120.0
13	5877.5
14	5635.0
15	5421.5
16	5208.0
17	5056.0
18	4904.0
19	5026.0
20	5148.0
21	4930.5
22	4713.0
23	5400.5
24	6088.0
25	5804.5
26	6501.5
27	7482.0
28	9191.0
29	10900.0
30	11559.0
31	12218.0
32	16780.5
33	21343.0
34	23511.0
35	25679.0
36	30117.5
37	34556.0
38	43182.0
39	51808.0
40	78792.0
41	105776.0
42	134850.0
43	163924.0
44	164241.5
45	164559.0
46	156860.0
47	149161.0
48	135269.0
49	121377.0
50	117283.0
51	113189.0
52	101988.0
53	90787.0
54	81939.0
55	73091.0
56	66020.0
57	58949.0
58	56839.5
59	54730.0
60	51329.0
61	47928.0
62	41997.5
63	36067.0
64	29352.0
65	22637.0
66	18648.5
67	14660.0
68	12310.5
69	9961.0
70	8644.0
71	7327.0
72	6038.0
73	4749.0
74	3483.5
75	1949.0
76	1680.0
77	1262.5
78	845.0
79	792.5
80	740.0
81	517.0
82	294.0
83	168.0
84	42.0
85	44.5
86	47.0
87	28.0
88	9.0
89	7.5
90	6.0
91	3.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1474694.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.781916300947607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.5081645172411	13.846451110731556
2	7.592274940017267	2.548258451543036
3	2.28357867522182	1.1496847858260422
4	1.0304919473282073	0.6917451843544995
5	0.5733017480852954	0.4810550975777189
6	0.3732351756623374	0.37581608871208944
7	0.26658156949580086	0.3131624710657645
8	0.19551827117666584	0.26249370097542263
9	0.16742428629810105	0.25287303234605596
>10	1.9974107601855755	8.213261112569606
>50	1.0741763080160796	13.325613122746526
>100	1.9280506005916298	56.89157374987816
>500	0.008975267290100689	0.9312558876217627
>1k	4.0796669500457673E-4	0.20142293937532907
>5k	4.0796669500457673E-4	0.5153332646764452
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7527	0.5104109733951586	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2942	0.1994990147108485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0357362273122424	0.0
2	0.0	0.0	0.0	0.12300856991348713	0.0
3	0.0	0.0	0.0	0.17779959774705803	0.0
4	0.0	0.0	0.0	0.293009939689183	0.0
5	0.0	0.0	0.0	0.4763022023552005	0.0
6	0.0	0.0	0.0	0.658712926207064	0.0
7	0.0	0.0	0.0	0.7648366372956017	0.0
8	0.0	0.0	0.0	1.0512011305396238	0.0
9	0.0	0.0	0.0	1.1586132445103865	0.0
10	0.0	0.0	0.0	1.349364681757707	0.0
11	0.0	0.0	0.0	1.532046648321618	0.0
12	0.0	0.0	0.0	1.699674644366899	0.0
13	0.0	0.0	0.0	1.7643660311901994	0.0
14	0.0	0.0	0.0	1.7913546810389138	0.0
15	0.0	0.0	0.0	1.8397036944613594	0.0
16	0.0	0.0	0.0	1.924873906044237	0.0
17	0.0	0.0	0.0	2.0271324084860995	0.0
18	0.0	0.0	0.0	2.20391484606298	0.0
19	0.0	0.0	0.0	2.270911795938683	0.0
20	0.0	0.0	0.0	2.3441473281914758	0.0
21	0.0	0.0	0.0	2.430266889266519	0.0
22	0.0	0.0	0.0	2.515369290171385	0.0
23	0.0	0.0	0.0	2.61369477328856	0.0
24	0.0	0.0	0.0	2.683404150284737	0.0
25	0.0	0.0	0.0	2.74267068286709	0.0
26	0.0	0.0	0.0	2.8053277493500346	0.0
27	0.0	0.0	0.0	2.860796883963724	0.0
28	0.0	0.0	0.0	2.923928625192752	0.0
29	0.0	0.0	0.0	2.983805453877211	0.0
30	0.0	0.0	0.0	3.0741292769889887	0.0
31	0.0	0.0	0.0	3.1626900224724586	0.0
32	0.0	0.0	0.0	3.242231947780353	0.0
33	0.0	0.0	0.0	3.3159421547792287	0.0
34	6.781067801184517E-5	0.0	0.0	3.3974505897494667	0.0
35	6.781067801184517E-5	0.0	0.0	3.5108300433852717	0.0
36	6.781067801184517E-5	0.0	0.0	3.598916114122659	0.0
37	6.781067801184517E-5	0.0	0.0	3.691613310964851	0.0
38	6.781067801184517E-5	0.0	0.0	3.771833343052864	0.0
39	6.781067801184517E-5	0.0	0.0	3.8571391759917653	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCTT	30	2.1659216E-6	45.000004	20
CGTGCCG	30	2.1659216E-6	45.000004	41
TAGCGAT	30	2.1659216E-6	45.000004	1
TGCGAAT	30	2.1659216E-6	45.000004	44
TGCGTAC	30	2.1659216E-6	45.000004	28
AGTCGAT	30	2.1659216E-6	45.000004	26
AACGTAG	40	6.8175723E-9	45.0	1
AGCGTAA	35	1.2122473E-7	45.0	1
ACTATCG	20	7.033937E-4	45.0	16
CGAACTT	55	1.8189894E-12	45.0	43
ACACGAA	20	7.033937E-4	45.0	23
GTCGATG	70	0.0	45.0	1
CTCCGAC	25	3.8913455E-5	45.0	32
CGGCCTA	35	1.2122473E-7	45.0	45
CGGTTAG	35	1.2122473E-7	45.0	24
TTCACGG	45	3.8562575E-10	45.0	2
ACTTCGT	25	3.8913455E-5	45.0	37
GTTAGGC	20	7.033937E-4	45.0	16
GTTAGCG	50	2.1827873E-11	45.0	1
CACGATA	20	7.033937E-4	45.0	39
>>END_MODULE