FastQCFastQC Report
Thu 26 May 2016
SRR1546274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546274_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1964732
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC49300.2509248080654257No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45130.229700539310196No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24050.12240855241325535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACA207.0345093E-445.00000411
CTACGGC207.0345093E-445.00000411
GTCGTAC207.0345093E-445.00000422
TATAGCG1300.045.0000041
TGTACGC207.0345093E-445.0000049
GCGTCTA207.0345093E-445.00000414
AATACGC207.0345093E-445.00000445
CCCGATA207.0345093E-445.00000427
GATCGAC453.8562575E-1045.09
CGAATCG351.2125201E-745.018
ATACCCG351.2125201E-745.01
ATCGATA351.2125201E-745.02
ATTCGTT302.1662927E-644.99999641
TCAATCG302.1662927E-644.99999624
CCGTAGA302.1662927E-644.99999641
CTACCGT302.1662927E-644.99999615
TACAGCG1200.044.9999961
TGCGTAG1650.042.2727281
CGGTCTA2250.042.031
CGACGGT2100.041.78571328
Produced by FastQC (version 0.11.5)