FastQCFastQC Report
Thu 26 May 2016
SRR1546273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546273_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2785771
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73790.2648817867656746No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG47250.16961193149042042No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT42810.1536737944360825No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30590.1098080208315759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA253.89224E-545.00000415
ATACGTA253.89224E-545.00000431
ACAATCG207.035019E-445.031
TATTACG351.2127566E-745.01
CGTAAAT700.045.016
TCCGATT302.16662E-644.99999613
TCTAACG800.042.18751
ATTGGGC7500.040.54
CGTTTTT68400.040.197371
CGGTCTA3450.039.78260831
CGCATAT800.039.37515
TCACGAC3550.039.29577625
CGGGTAT1800.038.756
ACCCGCT9500.038.60526334
ACTACGT356.2514937E-638.57142644
TTACGAG356.2514937E-638.5714261
TTTAGCG2400.038.4374961
CGAATAT5750.038.34782414
AACGGGA7050.038.2978744
ACGTAGG2350.038.297872
Produced by FastQC (version 0.11.5)