FastQCFastQC Report
Thu 26 May 2016
SRR1546272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546272_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences950472
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39890.41968621905747877No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36920.3884385863023845No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20930.2202063816714222No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11930.12551658544386368No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCG10000.10521088469728725No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATCG207.032669E-445.00000425
CCCCGTA207.032669E-445.00000440
ATTCACG207.032669E-445.0000041
AAGCACG207.032669E-445.0000041
CTACCGT207.032669E-445.00000437
CGTAATC207.032669E-445.00000433
ACCGTAT207.032669E-445.00000441
TATCGCG207.032669E-445.0000041
TAGCCGT351.2116288E-745.044
GTATGCG253.8902952E-545.01
TATTCCG253.8902952E-545.01
CGACAAA302.165105E-644.99999612
TAGTTCG302.165105E-644.9999961
CCGATCG2200.043.97727240
ATAACGG700.041.7857132
AGGGCGT700.041.7857136
CGATCGA2100.041.78571341
CGATGAA9300.041.61290419
CGGTCTA1250.041.39999831
TTCACGG603.6379788E-1241.2499962