##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546272_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	950472
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.918478398101154	31.0	31.0	34.0	31.0	34.0
2	32.24209761045039	33.0	31.0	34.0	31.0	34.0
3	32.320232473970826	34.0	31.0	34.0	31.0	34.0
4	36.01792688264357	37.0	35.0	37.0	35.0	37.0
5	35.821306677103586	37.0	35.0	37.0	35.0	37.0
6	35.977900453669335	37.0	35.0	37.0	35.0	37.0
7	36.1352538528226	37.0	35.0	37.0	35.0	37.0
8	36.13764529623177	37.0	35.0	37.0	35.0	37.0
9	37.873252447205175	39.0	38.0	39.0	35.0	39.0
10	37.590672844649816	39.0	37.0	39.0	35.0	39.0
11	37.39766137245495	39.0	37.0	39.0	35.0	39.0
12	36.65877900664091	39.0	35.0	39.0	33.0	39.0
13	36.32225462717471	38.0	35.0	39.0	33.0	39.0
14	37.44979231371361	40.0	35.0	41.0	33.0	41.0
15	37.58042635658915	39.0	35.0	41.0	33.0	41.0
16	37.69715783316079	39.0	35.0	41.0	34.0	41.0
17	37.63256255839204	39.0	35.0	41.0	34.0	41.0
18	37.645572936393705	39.0	35.0	41.0	34.0	41.0
19	37.55613631963909	39.0	35.0	41.0	34.0	41.0
20	37.42827037513993	39.0	35.0	41.0	34.0	41.0
21	37.347839810115396	39.0	35.0	41.0	33.0	41.0
22	37.26639080372699	39.0	35.0	41.0	33.0	41.0
23	37.15151103872602	39.0	35.0	41.0	33.0	41.0
24	37.096818212424985	38.0	35.0	41.0	33.0	41.0
25	36.99600619481689	38.0	35.0	41.0	33.0	41.0
26	36.98788075819172	38.0	35.0	41.0	33.0	41.0
27	36.927782196634936	38.0	35.0	41.0	33.0	41.0
28	36.92168101743134	38.0	35.0	41.0	33.0	41.0
29	36.9273245292865	38.0	35.0	41.0	33.0	41.0
30	36.87893067865229	38.0	35.0	41.0	33.0	41.0
31	36.74000496595376	38.0	35.0	41.0	33.0	41.0
32	36.51436865052311	38.0	35.0	41.0	32.0	41.0
33	36.31769794375847	38.0	35.0	41.0	31.0	41.0
34	36.12988914981188	38.0	35.0	41.0	31.0	41.0
35	35.94905057697649	38.0	35.0	41.0	31.0	41.0
36	35.8318519640768	38.0	35.0	40.0	30.0	41.0
37	35.73677288757586	38.0	35.0	40.0	30.0	41.0
38	35.67005130082738	38.0	35.0	40.0	30.0	41.0
39	35.62146281005648	38.0	35.0	40.0	30.0	41.0
40	35.46212828994437	37.0	35.0	40.0	29.0	41.0
41	35.436444208772066	37.0	35.0	40.0	29.0	41.0
42	35.36510386418537	37.0	35.0	40.0	29.0	41.0
43	35.3132727739481	37.0	35.0	40.0	29.0	41.0
44	35.04072608135747	36.0	34.0	40.0	28.0	41.0
45	35.07837158801101	36.0	34.0	40.0	28.0	41.0
46	35.03246597479989	36.0	34.0	40.0	28.0	41.0
47	34.96955828262168	36.0	34.0	40.0	28.0	41.0
48	34.91545253305726	36.0	34.0	40.0	28.0	41.0
49	34.845797666843424	36.0	34.0	40.0	28.0	41.0
50	34.74523605114091	35.0	34.0	40.0	28.0	41.0
51	34.68899978116136	35.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	4.0
14	6.0
15	16.0
16	39.0
17	102.0
18	247.0
19	479.0
20	891.0
21	1682.0
22	2950.0
23	4694.0
24	6762.0
25	8954.0
26	11212.0
27	12391.0
28	12448.0
29	13215.0
30	15391.0
31	19309.0
32	25874.0
33	38455.0
34	85420.0
35	152132.0
36	61906.0
37	85332.0
38	139627.0
39	250757.0
40	174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.352586925232934	24.471946569704315	28.65386881465209	13.521597690410658
2	30.720000168337414	27.511489028608942	29.149622503345707	12.618888299707933
3	29.6501106818507	25.97235899637233	31.117486890723768	13.260043431053203
4	26.873069380265807	27.284128306778104	31.002807026403723	14.839995286552366
5	24.564216515583837	32.50458719457281	28.804530801538604	14.126665488304758
6	22.017481840601302	40.91924854177714	26.2914636096592	10.771806007962361
7	84.96694274002812	5.219617200717117	7.87619203932362	1.93724801993115
8	86.34783560168002	5.391216153658393	6.175668509961366	2.0852797347002334
9	81.01038221010192	6.9931570840592885	7.934478869445917	4.061981836392866
10	50.48428570226161	25.135932463028894	13.132317417030695	11.247464417678795
11	45.399548855726415	21.379693457566347	19.743769411408227	13.476988275299009
12	39.32393589711217	21.829469989647247	23.85477952007003	14.991814593170552
13	23.35744766810595	37.24044474745179	23.87098199631341	15.531125588128846
14	16.49043843479871	40.84360191567979	26.786270400390542	15.879689249130957
15	15.70893198326726	22.988893939011355	45.031942024594095	16.270232053127287
16	17.538864900807177	19.452650893450834	42.72856012591639	20.279924079825605
17	17.67069413933288	20.46972451581951	28.67722563105489	33.182355713792724
18	22.137737881810303	23.441932008517874	32.17369896219983	22.246631147471994
19	29.439057647147944	23.70306542433654	25.644311457886186	21.213565470629327
20	32.32972670420591	22.70629750271444	24.123277697817507	20.840698095262145
21	23.631837655396478	28.039647669789325	27.15997946283531	21.168535211978888
22	23.332828321086787	25.04029576883906	25.680609213106752	25.946266696967403
23	21.74267100977199	28.95361462515466	25.035771700797078	24.267942664276276
24	21.09678138861534	24.932244190254945	35.443232415052734	18.52774200607698
25	19.12081576311559	26.775959733690208	32.75761937226977	21.345605130924426
26	18.45525170652055	33.00644311457886	27.434159028356436	21.10414615054415
27	21.098464762770497	32.05586277133887	27.526113341581866	19.319559124308764
28	16.78208300717959	29.990047050307638	34.849211760051844	18.378658182460924
29	17.88237843934382	25.184960735297828	35.36011581614187	21.572545009216473
30	19.789430935366852	30.449818616434783	29.14088999991583	20.619860448282537
31	28.6417695629119	26.49357371916269	24.374942134013416	20.489714583911994
32	30.422884629952275	26.514405474332754	25.898606166199528	17.16410372951544
33	28.734355141445512	27.79166561455782	23.585755287899065	19.888223956097605
34	21.11666624582313	27.984938009746735	27.005003829676205	23.893391914753934
35	22.991524211128787	25.52805343029568	28.87323350924593	22.607188849329596
36	28.51109764411787	27.85689636307014	24.80493901977123	18.82706697304076
37	21.579383716721797	32.52247304497134	26.72503766549672	19.17310557281014
38	22.346476277049717	32.13340319336077	22.449372522283664	23.070748007305845
39	22.451371529092913	30.71831679418226	24.915094816049287	21.91521686067554
40	24.043843479871054	26.61572355629624	24.777163346211147	24.56326961762156
41	18.44715046839886	26.692211869471166	26.174153473221722	28.686484188908246
42	22.5061864000202	27.609861205800907	23.5263111380451	26.357641256133796
43	23.40026849817775	26.364164330985023	25.06912355124612	25.166443619591107
44	20.6252262034021	29.752165240007074	27.95179658106709	21.67081197552374
45	18.822227272344687	35.25963942125597	23.621000934272658	22.29713237212669
46	21.90311760893535	32.40326911260931	25.529947226220234	20.1636660522351
47	23.077271082157075	28.220399969699265	26.15837184051713	22.54395710762653
48	22.906724238062772	25.85094563543166	29.009692026698314	22.232638099807254
49	22.26146588221431	25.07985506148524	30.310729826864968	22.34794922943548
50	20.29286501864337	32.259130200574035	26.068311323216253	21.379693457566347
51	20.769996380745567	32.22988157462818	24.233959548519053	22.7661624961072
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1002.0
1	1449.0
2	1896.0
3	4651.5
4	7407.0
5	5493.5
6	3580.0
7	3741.5
8	3903.0
9	4198.5
10	4494.0
11	4650.0
12	4806.0
13	4566.0
14	4326.0
15	4171.5
16	4017.0
17	4074.0
18	4131.0
19	3995.5
20	3860.0
21	4029.5
22	4199.0
23	4341.0
24	4483.0
25	5160.0
26	7310.0
27	8783.0
28	10678.5
29	12574.0
30	13348.5
31	14123.0
32	15876.5
33	17630.0
34	19601.0
35	21572.0
36	23789.0
37	26006.0
38	31891.5
39	37777.0
40	48293.0
41	58809.0
42	68345.0
43	77881.0
44	80619.5
45	83358.0
46	82127.0
47	80896.0
48	76685.0
49	72474.0
50	66527.0
51	60580.0
52	59708.5
53	58837.0
54	55071.0
55	51305.0
56	48186.0
57	45067.0
58	44655.5
59	44244.0
60	40374.5
61	36505.0
62	32007.5
63	27510.0
64	23339.0
65	19168.0
66	15964.5
67	12761.0
68	10427.5
69	8094.0
70	7259.5
71	6425.0
72	5599.0
73	4773.0
74	3670.5
75	2020.5
76	1473.0
77	1059.5
78	646.0
79	542.0
80	438.0
81	280.0
82	122.0
83	109.0
84	96.0
85	55.0
86	14.0
87	15.5
88	17.0
89	10.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	950472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.297301196175553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.44623732215743	14.353544319901825
2	5.44885714881865	1.9939876085373838
3	1.985394747151951	1.0898209704583217
4	1.1705050010856854	0.8566833022597833
5	0.794831239492426	0.727163329456123
6	0.6479763753237714	0.7113731344383084
7	0.5250602817357191	0.672503028474707
8	0.46611543395150323	0.6822923589757381
9	0.41673850652347566	0.6862670976513964
>10	7.074866571232441	33.22912823409027
>50	2.5612241271781127	32.56571599723144
>100	0.45638679754296	10.75012959281967
>500	0.003483868683534046	0.5162314541830387
>1k	0.0023225791223560307	1.1651595715219973
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3989	0.41968621905747877	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3692	0.3884385863023845	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2093	0.2202063816714222	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1193	0.12551658544386368	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCG	1000	0.10521088469728725	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017465006859749683	0.0
2	0.0	0.0	0.0	0.0669141226674747	0.0
3	0.0	0.0	0.0	0.10184413638697405	0.0
4	0.0	0.0	0.0	0.1773855515996263	0.0
5	0.0	0.0	0.0	0.3202619330185424	0.0
6	0.0	0.0	0.0	0.43083857283539123	0.0
7	0.0	0.0	0.0	0.5042757703540978	0.0
8	0.0	0.0	0.0	0.6558846552028886	0.0
9	0.0	0.0	0.0	0.7191163969059583	0.0
10	0.0	0.0	0.0	0.9394279894620778	0.0
11	0.0	0.0	0.0	1.082725214419783	0.0
12	0.0	0.0	0.0	1.2542189564763613	0.0
13	0.0	0.0	0.0	1.3062983444015184	0.0
14	0.0	0.0	0.0	1.3286030519573433	0.0
15	0.0	0.0	0.0	1.3706874058362581	0.0
16	0.0	0.0	0.0	1.444650657778451	0.0
17	0.0	0.0	0.0	1.517877433527763	0.0
18	0.0	0.0	0.0	1.624456059726115	0.0
19	0.0	0.0	0.0	1.695157774242692	0.0
20	0.0	0.0	0.0	1.7541810805578701	0.0
21	0.0	0.0	0.0	1.8213056249947395	0.0
22	0.0	0.0	0.0	1.896636618437997	0.0
23	0.0	0.0	0.0	1.9727040880741358	0.0
24	0.0	0.0	0.0	2.029202333156579	0.0
25	0.0	0.0	0.0	2.082754673467498	0.0
26	0.0	0.0	0.0	2.133571530776288	0.0
27	0.0	0.0	0.0	2.1885968234729694	0.0
28	0.0	0.0	0.0	2.243727327054348	0.0
29	0.0	0.0	0.0	2.2992786741745155	0.0
30	0.0	0.0	0.0	2.375030511156562	0.0
31	0.0	0.0	0.0	2.448678130444663	0.0
32	0.0	0.0	0.0	2.517801681690781	0.0
33	0.0	0.0	0.0	2.5858731240899258	0.0
34	0.0	0.0	0.0	2.6563644168371083	0.0
35	0.0	0.0	0.0	2.7549470157984666	0.0
36	0.0	0.0	0.0	2.835643764361286	0.0
37	0.0	0.0	0.0	2.9154988258465266	0.0
38	0.0	0.0	0.0	2.9908298192897846	0.0
39	0.0	0.0	0.0	3.0721578331607873	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATATCG	20	7.032669E-4	45.000004	25
CCCCGTA	20	7.032669E-4	45.000004	40
ATTCACG	20	7.032669E-4	45.000004	1
AAGCACG	20	7.032669E-4	45.000004	1
CTACCGT	20	7.032669E-4	45.000004	37
CGTAATC	20	7.032669E-4	45.000004	33
ACCGTAT	20	7.032669E-4	45.000004	41
TATCGCG	20	7.032669E-4	45.000004	1
TAGCCGT	35	1.2116288E-7	45.0	44
GTATGCG	25	3.8902952E-5	45.0	1
TATTCCG	25	3.8902952E-5	45.0	1
CGACAAA	30	2.165105E-6	44.999996	12
TAGTTCG	30	2.165105E-6	44.999996	1
CCGATCG	220	0.0	43.977272	40
ATAACGG	70	0.0	41.785713	2
AGGGCGT	70	0.0	41.785713	6
CGATCGA	210	0.0	41.785713	41
CGATGAA	930	0.0	41.612904	19
CGGTCTA	125	0.0	41.399998	31
TTCACGG	60	3.6379788E-12	41.249996	2
>>END_MODULE