FastQCFastQC Report
Thu 26 May 2016
SRR1546271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546271_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1813778
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC60460.3333373764595226No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44980.2479906581731612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACA207.034365E-445.00000423
CTATCCG207.034365E-445.0000041
CCGCTTA207.034365E-445.00000440
TAGCCGT600.045.00000444
AGTATCA207.034365E-445.00000434
CACTCGA302.166198E-645.00000438
CCGGTCA406.8193913E-945.00000431
GACGATT207.034365E-445.00000427
ATACGCC406.8193913E-945.00000428
ATTGCGA207.034365E-445.0000041
TACGTTC207.034365E-445.00000432
GATCGAC253.8917013E-545.024
GGTCGAT502.1827873E-1145.08
CACGATA253.8917013E-545.031
TCGTACT253.8917013E-545.043
ATCGGTC453.8562575E-1045.026
GATTCGC253.8917013E-545.09
GCATGCG502.1827873E-1145.01
TCGGTAA253.8917013E-545.035
TGGTCGA253.8917013E-545.028