##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546271_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1813778
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248840817343687	31.0	31.0	33.0	30.0	34.0
2	31.480689477984626	31.0	31.0	34.0	30.0	34.0
3	31.571776700345907	31.0	31.0	34.0	30.0	34.0
4	35.34034705460095	37.0	35.0	37.0	33.0	37.0
5	35.068533745585185	37.0	35.0	37.0	32.0	37.0
6	35.2155666239198	37.0	35.0	37.0	32.0	37.0
7	35.73844263189872	37.0	35.0	37.0	35.0	37.0
8	35.82097643702813	37.0	35.0	37.0	35.0	37.0
9	37.614751639947116	39.0	37.0	39.0	35.0	39.0
10	36.873729861096564	39.0	37.0	39.0	32.0	39.0
11	36.48610689952133	39.0	35.0	39.0	32.0	39.0
12	35.44346441515996	37.0	35.0	39.0	31.0	39.0
13	34.887073831527346	37.0	34.0	39.0	28.0	39.0
14	35.8172896572789	38.0	34.0	40.0	29.0	41.0
15	36.18316243774045	38.0	35.0	40.0	30.0	41.0
16	36.368783831317835	38.0	35.0	40.0	31.0	41.0
17	36.31102428191322	37.0	35.0	40.0	31.0	41.0
18	36.26436035722123	37.0	35.0	40.0	31.0	41.0
19	36.17657839051968	37.0	35.0	40.0	31.0	41.0
20	36.087753297261294	37.0	34.0	40.0	31.0	41.0
21	35.91674339417503	36.0	34.0	40.0	30.0	41.0
22	35.803603307571265	36.0	34.0	40.0	30.0	41.0
23	35.82449781616052	36.0	34.0	40.0	30.0	41.0
24	35.728812456651255	36.0	34.0	40.0	30.0	41.0
25	35.586488533877905	36.0	34.0	40.0	30.0	41.0
26	35.522028605485346	36.0	34.0	40.0	30.0	41.0
27	35.45658785143496	36.0	34.0	40.0	29.0	41.0
28	35.452200324405744	36.0	34.0	40.0	29.0	41.0
29	35.51718898343678	36.0	34.0	40.0	30.0	41.0
30	35.44052579753421	36.0	34.0	40.0	29.0	41.0
31	35.29132120910056	36.0	34.0	40.0	29.0	41.0
32	35.112601983263666	35.0	34.0	40.0	29.0	41.0
33	34.95119523999078	36.0	34.0	40.0	27.0	41.0
34	34.8292740346393	35.0	34.0	40.0	27.0	41.0
35	34.66276357966631	35.0	33.0	40.0	27.0	41.0
36	34.49709997585151	35.0	33.0	40.0	25.0	41.0
37	34.42512258942384	35.0	33.0	40.0	25.0	41.0
38	34.45584520266537	35.0	33.0	40.0	26.0	41.0
39	34.41495872151939	35.0	33.0	40.0	25.0	41.0
40	34.26246100680458	35.0	33.0	40.0	24.0	41.0
41	34.223413780517795	35.0	33.0	40.0	24.0	41.0
42	34.15747958129385	35.0	33.0	40.0	24.0	41.0
43	34.099625202202255	35.0	33.0	40.0	24.0	41.0
44	33.875000689169234	35.0	33.0	39.0	23.0	41.0
45	33.87532487437823	35.0	33.0	39.0	23.0	41.0
46	33.83763613849104	35.0	33.0	39.0	23.0	41.0
47	33.77343313238996	35.0	33.0	39.0	23.0	41.0
48	33.72597473340177	35.0	33.0	39.0	23.0	41.0
49	33.650636957775426	35.0	33.0	39.0	24.0	41.0
50	33.58289548114488	35.0	33.0	39.0	24.0	41.0
51	33.468302625789924	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	3.0
14	12.0
15	64.0
16	144.0
17	511.0
18	1057.0
19	2176.0
20	3743.0
21	5872.0
22	8833.0
23	12740.0
24	17623.0
25	23653.0
26	30088.0
27	35709.0
28	40983.0
29	47257.0
30	56410.0
31	68981.0
32	86592.0
33	112720.0
34	194726.0
35	278625.0
36	123824.0
37	150204.0
38	209010.0
39	302107.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.837701196066995	24.09412838836947	29.706943187093458	13.361227228470076
2	29.721608708452745	24.846866595581158	32.465053606339914	12.966471089626182
3	28.85485434270346	24.963143229215483	32.273409424968214	13.90859300311284
4	25.44539629436458	28.011697131622505	31.501374479125893	15.041532094887025
5	23.29584987798948	32.305221476939295	30.262854660272648	14.13607398479858
6	22.309510866269193	41.04074478795089	26.001362901082715	10.6483814446972
7	88.24133934803487	4.934341468470784	5.352143426593552	1.4721757569007894
8	90.83708149508925	2.928362787507622	4.941508828533591	1.2930468888695308
9	87.57962661362086	4.318775506153454	5.949019119208635	2.1525787610170597
10	56.87311236546038	22.69561103949877	10.478625278286538	9.95265131675431
11	49.67366458298645	19.245133638185045	17.750297996778	13.330903782050504
12	43.024780320414074	21.044637215800392	22.13567481797662	13.794907645808912
13	21.256129471192175	41.097256665369194	22.9776190912008	14.668994772237836
14	15.399128228482207	42.284392025925996	27.398281377324018	14.918198368267783
15	14.664584089122263	21.68600567434383	48.69013738175234	14.959272854781567
16	17.23198759715908	17.668700359139873	44.97716920152301	20.12214284217804
17	17.592560941857272	18.15707324711183	27.22036544714954	37.030000363881356
18	23.372595764200472	21.9048858239542	32.959601450673674	21.762916961171655
19	30.761316985871478	23.70918601945773	24.38159466042702	21.14790233424377
20	33.64182386157512	22.671021481129443	23.763106620545624	19.924048036749813
21	24.567890888521088	26.88708320422896	26.513774012034546	22.0312518952154
22	24.01021514209567	23.732728040587105	25.072583304020668	27.184473513296552
23	20.31511022848441	28.973391451434523	24.52703693616308	26.18446138391799
24	21.76473636795683	23.658849098401237	36.29595242637191	18.28046210727002
25	19.46914120691727	24.63383060109892	34.11338102016895	21.783647171814852
26	18.227037708032626	34.32851208913109	25.80012548393464	21.644324718901654
27	18.933243208375007	33.498421526780014	28.443999210487725	19.124336054357258
28	15.877466812366231	28.30616536312603	37.37000889855319	18.446358925954552
29	16.614106026206073	24.568662758066314	37.022116267812265	21.795114947915344
30	19.882587615463414	29.363461239468116	31.220799899436425	19.533151245632048
31	30.78226773067046	25.65104439462823	23.579732470015625	19.986955404685688
32	31.756367096745024	25.907194816565205	24.48827805828497	17.8481600284048
33	29.61464964290007	27.85677188718796	23.157740362933062	19.370838106978912
34	21.181258125305302	27.751797629037288	27.33195572997357	23.73498851568384
35	21.783702305353795	26.588700491460365	29.67535166927816	21.95224553390768
36	30.350406720116794	25.91728425419208	25.165317916525616	18.56699110916551
37	21.116421083506363	32.06505978129628	28.00436437094286	18.8141547642545
38	21.213842046821608	32.22412004115167	22.875511777075257	23.686526134951468
39	21.352502897267474	31.332831250572013	25.93801446483528	21.37665138732524
40	24.850670809768342	25.789705245074096	24.82404131045806	24.535582634699505
41	18.578624285882835	24.12583017326266	26.947013361061828	30.348532179792677
42	23.08297928412408	25.66802552462319	23.758199735579545	27.490795455673183
43	23.88649548070381	25.080301999472926	25.241236799652437	25.791965720170822
44	20.49920111502069	30.178334945070457	27.275223318399494	22.04724062150936
45	18.55083698225472	36.48368212647854	22.623772038253858	22.341708853012882
46	23.09516379623085	32.51020797473561	24.85304155194296	19.54158667709058
47	21.869600359029604	27.776938522796062	26.24648661523075	24.10697450294358
48	24.377294244389336	23.6136395964666	29.753916962274324	22.255149196869738
49	22.333659356326958	23.31674548924951	31.292032431752947	23.05756272267058
50	20.82581219972896	31.890617264075317	25.829180858958484	21.454389677237238
51	18.006283018098134	33.33500571734799	24.49274387493949	24.165967389614384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1059.0
1	1600.5
2	2142.0
3	5667.5
4	9193.0
5	7183.5
6	5174.0
7	5561.0
8	5948.0
9	6238.5
10	6529.0
11	6684.0
12	6839.0
13	6744.0
14	6649.0
15	6228.0
16	5807.0
17	5472.5
18	5138.0
19	5168.0
20	5198.0
21	5499.0
22	5800.0
23	6620.5
24	7441.0
25	7330.0
26	8290.5
27	9362.0
28	12765.0
29	16168.0
30	18549.5
31	20931.0
32	22815.0
33	24699.0
34	31192.0
35	37685.0
36	42145.5
37	46606.0
38	57557.5
39	68509.0
40	96491.5
41	124474.0
42	157329.0
43	190184.0
44	188293.5
45	186403.0
46	174939.5
47	163476.0
48	146856.5
49	130237.0
50	128310.0
51	126383.0
52	118993.5
53	111604.0
54	105390.5
55	99177.0
56	93174.0
57	87171.0
58	82724.5
59	78278.0
60	73763.0
61	69248.0
62	58190.5
63	47133.0
64	40531.5
65	33930.0
66	27512.5
67	21095.0
68	18112.5
69	15130.0
70	13108.0
71	11086.0
72	9147.0
73	7208.0
74	5510.5
75	2799.5
76	1786.0
77	1384.0
78	982.0
79	753.0
80	524.0
81	366.0
82	208.0
83	134.5
84	61.0
85	50.0
86	39.0
87	35.0
88	31.0
89	17.5
90	4.0
91	4.0
92	4.0
93	8.0
94	12.0
95	6.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1813778.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.805312888061955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.96734445525865	13.6068155728611
2	8.165390817665116	2.7444389508834064
3	2.569020446600419	1.2951957726284606
4	1.1933860769646565	0.8022090567859136
5	0.6606363071991798	0.5551099923848016
6	0.38002298518647243	0.38318431024284016
7	0.260817458957098	0.3068183303110302
8	0.20351531475435392	0.27361108335674633
9	0.17593404368557056	0.26609639866181767
>10	2.278245588934929	9.550777942973724
>50	1.2724386945061312	15.691254944792815
>100	1.8662655234546306	53.2949371558488
>500	0.006317307134444644	0.6403943715540035
>1k	3.32489849181297E-4	0.25133006572287825
>5k	3.32489849181297E-4	0.33782605099166785
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6046	0.3333373764595226	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4498	0.2479906581731612	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03291472274997271	0.0
2	0.0	0.0	0.0	0.09025360325243773	0.0
3	0.0	0.0	0.0	0.13110755561044404	0.0
4	0.0	0.0	0.0	0.20090661591440628	0.0
5	0.0	0.0	0.0	0.3194437246454638	0.0
6	0.0	0.0	0.0	0.43489335519561934	0.0
7	0.0	0.0	0.0	0.49868285975461163	0.0
8	0.0	0.0	0.0	0.6565301817532245	0.0
9	0.0	0.0	0.0	0.7108367176137322	0.0
10	0.0	0.0	0.0	0.8202216588799732	0.0
11	0.0	0.0	0.0	0.9751469033145181	0.0
12	0.0	0.0	0.0	1.099914101946324	0.0
13	0.0	0.0	0.0	1.1518498956322107	0.0
14	0.0	0.0	0.0	1.171697969652295	0.0
15	0.0	0.0	0.0	1.2063218321095526	0.0
16	0.0	0.0	0.0	1.2841703890994378	0.0
17	0.0	0.0	0.0	1.3740931911182075	0.0
18	0.0	0.0	0.0	1.4985847220552901	0.0
19	0.0	0.0	0.0	1.5595072825891592	0.0
20	0.0	0.0	0.0	1.6183347686431304	0.0
21	0.0	0.0	0.0	1.6979475988792454	0.0
22	0.0	0.0	0.0	1.7794349694394793	0.0
23	0.0	0.0	0.0	1.8812114823313548	0.0
24	0.0	0.0	0.0	1.953932620199385	0.0
25	0.0	0.0	0.0	2.0156821838174244	0.0
26	0.0	0.0	0.0	2.0759982754229016	0.0
27	0.0	0.0	0.0	2.134660360860039	0.0
28	1.102670778893558E-4	0.0	0.0	2.2020335454504356	0.0
29	1.102670778893558E-4	0.0	0.0	2.274699549779521	0.0
30	1.102670778893558E-4	0.0	0.0	2.3674341622844692	0.0
31	1.102670778893558E-4	0.0	0.0	2.4565299612190685	0.0
32	1.102670778893558E-4	0.0	0.0	2.5368595274614645	0.0
33	1.102670778893558E-4	0.0	0.0	2.6198906371121495	0.0
34	1.102670778893558E-4	0.0	0.0	2.709262103741472	0.0
35	1.102670778893558E-4	0.0	0.0	2.828240280784087	0.0
36	1.102670778893558E-4	0.0	0.0	2.917777148030244	0.0
37	1.654006168340337E-4	0.0	0.0	3.011724698391975	0.0
38	2.205341557787116E-4	0.0	0.0	3.0987254228466767	0.0
39	2.205341557787116E-4	0.0	0.0	3.202817544374229	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACA	20	7.034365E-4	45.000004	23
CTATCCG	20	7.034365E-4	45.000004	1
CCGCTTA	20	7.034365E-4	45.000004	40
TAGCCGT	60	0.0	45.000004	44
AGTATCA	20	7.034365E-4	45.000004	34
CACTCGA	30	2.166198E-6	45.000004	38
CCGGTCA	40	6.8193913E-9	45.000004	31
GACGATT	20	7.034365E-4	45.000004	27
ATACGCC	40	6.8193913E-9	45.000004	28
ATTGCGA	20	7.034365E-4	45.000004	1
TACGTTC	20	7.034365E-4	45.000004	32
GATCGAC	25	3.8917013E-5	45.0	24
GGTCGAT	50	2.1827873E-11	45.0	8
CACGATA	25	3.8917013E-5	45.0	31
TCGTACT	25	3.8917013E-5	45.0	43
ATCGGTC	45	3.8562575E-10	45.0	26
GATTCGC	25	3.8917013E-5	45.0	9
GCATGCG	50	2.1827873E-11	45.0	1
TCGGTAA	25	3.8917013E-5	45.0	35
TGGTCGA	25	3.8917013E-5	45.0	28
>>END_MODULE