FastQCFastQC Report
Thu 26 May 2016
SRR1546270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546270_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1356268
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC55710.41075952540353383No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT30210.2227435875505431No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28830.21256860738438124No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAG207.033736E-445.019
TCGTTGC207.033736E-445.043
ACACGGT253.8911792E-545.013
CTATCGA207.033736E-445.024
GAATCGG207.033736E-445.022
GCAAACG253.8911792E-545.010
TATGCGT207.033736E-445.020
ACGATAT207.033736E-445.014
ACTAGCG207.033736E-445.021
ACCTACG207.033736E-445.039
TCCGTAC207.033736E-445.013
GTTCACG253.8911792E-545.01
TTACGTC253.8911792E-545.016
CCGAACT453.8562575E-1045.042
GGACCGT406.8157533E-945.08
GTGACGT207.033736E-445.014
CGCCGAA207.033736E-445.028
GCGTCTA253.8911792E-545.09
TATCGTG351.2121382E-745.01
TACGTCC253.8911792E-545.017