##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546270_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1356268
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1494881542586	31.0	31.0	33.0	30.0	34.0
2	31.384236743770405	31.0	31.0	34.0	30.0	34.0
3	31.484315784196045	31.0	31.0	34.0	30.0	34.0
4	35.254084738414534	37.0	35.0	37.0	33.0	37.0
5	34.97561912542359	37.0	35.0	37.0	32.0	37.0
6	35.10818289600581	37.0	35.0	37.0	32.0	37.0
7	35.65220959279434	37.0	35.0	37.0	35.0	37.0
8	35.71713997528512	37.0	35.0	37.0	35.0	37.0
9	37.4519269053019	39.0	37.0	39.0	35.0	39.0
10	36.77268946845314	39.0	37.0	39.0	32.0	39.0
11	36.43216163767043	38.0	35.0	39.0	32.0	39.0
12	35.387256058537105	37.0	35.0	39.0	30.0	39.0
13	34.77118017972849	37.0	33.0	39.0	27.0	39.0
14	35.684793860800376	37.0	34.0	40.0	27.0	41.0
15	36.05061683973964	37.0	34.0	40.0	30.0	41.0
16	36.26082898070293	37.0	35.0	40.0	31.0	41.0
17	36.22074103348306	37.0	35.0	40.0	31.0	41.0
18	36.19938094830815	37.0	35.0	40.0	31.0	41.0
19	36.12858889246078	37.0	35.0	40.0	31.0	41.0
20	36.030432038505666	36.0	34.0	40.0	30.0	41.0
21	35.8580944179174	36.0	34.0	40.0	30.0	41.0
22	35.74796058006235	36.0	34.0	40.0	30.0	41.0
23	35.7599840149587	36.0	34.0	40.0	30.0	41.0
24	35.62971846272271	36.0	34.0	40.0	30.0	41.0
25	35.50174301834151	36.0	34.0	40.0	29.0	41.0
26	35.44457732542536	36.0	34.0	40.0	29.0	41.0
27	35.35584781178941	36.0	34.0	40.0	29.0	41.0
28	35.36463147401546	36.0	34.0	40.0	29.0	41.0
29	35.40083007193269	36.0	34.0	40.0	29.0	41.0
30	35.3265726242896	36.0	34.0	40.0	29.0	41.0
31	35.204394706650895	36.0	34.0	40.0	29.0	41.0
32	35.02654342652042	35.0	34.0	40.0	29.0	41.0
33	34.856283566374785	35.0	34.0	40.0	27.0	41.0
34	34.746292768095984	35.0	34.0	40.0	27.0	41.0
35	34.60457815122085	35.0	33.0	40.0	26.0	41.0
36	34.39813075291904	35.0	33.0	40.0	25.0	41.0
37	34.299644318084624	35.0	33.0	40.0	24.0	41.0
38	34.32876909283416	35.0	33.0	40.0	25.0	41.0
39	34.28141193333471	35.0	33.0	40.0	24.0	41.0
40	34.07532803251275	35.0	33.0	40.0	23.0	41.0
41	34.03577611504511	35.0	33.0	40.0	23.0	41.0
42	33.989914972557045	35.0	33.0	40.0	23.0	41.0
43	33.93499219918187	35.0	33.0	40.0	23.0	41.0
44	33.728435677904365	35.0	33.0	39.0	23.0	41.0
45	33.73346713186479	35.0	33.0	39.0	23.0	41.0
46	33.66412759130201	35.0	33.0	39.0	23.0	41.0
47	33.63969731645958	35.0	33.0	39.0	23.0	41.0
48	33.56427638195401	35.0	33.0	39.0	23.0	41.0
49	33.52146404692878	35.0	33.0	39.0	24.0	41.0
50	33.45091825509412	35.0	32.0	39.0	24.0	41.0
51	33.36727696885866	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	8.0
14	25.0
15	53.0
16	177.0
17	452.0
18	982.0
19	1825.0
20	3075.0
21	4884.0
22	7275.0
23	10252.0
24	14054.0
25	18671.0
26	23570.0
27	27848.0
28	31457.0
29	36070.0
30	43396.0
31	53279.0
32	65994.0
33	85616.0
34	145664.0
35	208113.0
36	90881.0
37	110213.0
38	150983.0
39	221341.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.521177230458875	24.966009667705794	27.608776436515498	13.904036665319833
2	28.73458637968307	27.776737340997503	29.7444900270448	13.744186252274623
3	28.481170388153377	26.69236463589792	30.68427478934842	14.14219018660029
4	25.36615182250116	28.342186057622833	30.39635234334217	15.895309776533843
5	22.633727257444693	33.32084809197002	30.026366470343618	14.019058180241665
6	20.797733191375155	40.59271471420103	27.200376326802665	11.40917576762115
7	85.67554495129282	5.6496208713912	6.349408818905998	2.3254253584099898
8	87.54449710529187	4.280201258158417	5.65566687409863	2.519634762451079
9	83.53857792117782	5.818097898055546	7.296198096541391	3.3471260842252417
10	53.65016353700006	22.981003754420218	12.94073147784951	10.42810123073021
11	48.84174809108524	20.271657224088454	18.14125231886323	12.745342365963069
12	45.925878956076524	21.09000581006114	20.61207666921287	12.372038564649465
13	22.630114402168303	43.38508318414945	19.362028743581654	14.6227736701006
14	14.662220151179561	45.430696587989985	23.888494014457322	16.018589246373136
15	13.15145679172553	23.595484078367992	48.59349332137896	14.65956580852752
16	13.984772920986119	18.39828116566932	47.22923492996959	20.38771098337497
17	14.141305405716276	19.463926008723938	27.495966873803702	38.89880171175608
18	19.869819239265397	24.72365343722627	33.92013967740889	21.486387646099445
19	29.027006461849723	26.647462006034207	22.312404333066915	22.01312719904916
20	32.490481232322814	23.130236796857258	23.372298100375442	21.006983870444486
21	20.734250162947145	29.319279080535704	27.044212500774183	22.902258255742964
22	22.196866695962743	26.676069921283997	23.035933900969425	28.091129481783838
23	19.802059769897983	31.862655463374495	22.26175062745711	26.07353413927041
24	19.900417911504217	23.783205089259646	38.80671076807828	17.50966623115785
25	16.858614964004165	26.887090162121353	33.898536277490884	22.355758596383605
26	16.49673958244241	35.99178038558751	25.176292591139806	22.33518744083028
27	17.635526311908855	35.732981977013395	26.41011953389743	20.22137217718032
28	14.719509713419471	29.737927902155032	36.59062958058436	18.951932803841128
29	14.91895407102431	24.29947473508186	36.61260163920405	24.16896955468978
30	17.24305225810828	31.78634311212828	30.370103843783085	20.60050078598035
31	27.998817342885037	29.234708774372027	22.047486190045035	20.718987692697905
32	30.005279192607954	27.063825143703163	23.786670481055367	19.14422518263352
33	27.221389872798003	30.28243680452536	22.326339631990137	20.169833690686502
34	18.799086906127698	28.807064680431893	26.54379517912389	25.850053234316523
35	19.362028743581654	26.508846334205334	29.934865380588498	24.194259541624515
36	29.369932786145508	26.984858449804904	23.84034718801889	19.804861576030696
37	19.305771425706425	33.84766137666007	27.953988444761656	18.892578752871852
38	20.192100676267522	32.878088991261315	23.918871491475137	23.01093884099603
39	19.624956129614503	32.34618821648819	26.356590290414577	21.672265363482733
40	23.984124081671172	26.477067954121164	24.69349715542946	24.845310808778205
41	16.292428929975493	25.69890316663078	27.07812910132806	30.930538802065666
42	21.708762574948317	27.170588703707526	22.377804386743623	28.742844334600537
43	22.38311307204771	28.11096331993382	24.27433221162779	25.231591396390684
44	19.740198839757333	30.76744419244574	26.02221684799759	23.470140119799332
45	16.81009947886406	37.40661875086635	20.998725915527018	24.784555854742575
46	21.0854344421604	34.69977909970596	24.296599197208813	19.91818726092483
47	20.733955235985807	29.534502030572128	26.210453981071584	23.521088752370474
48	22.671699103716964	24.13394697803089	29.83171467586052	23.362639242391623
49	20.305057702459987	23.962668145233835	31.25296770254846	24.47930644975772
50	18.517947780232223	33.42090206360395	25.919361070230956	22.14178908593287
51	17.058649175531677	34.296171553114874	22.712988878304287	25.932190393049158
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	976.0
1	1266.0
2	1556.0
3	3599.0
4	5642.0
5	4518.5
6	3395.0
7	3791.5
8	4188.0
9	4590.0
10	4992.0
11	5297.0
12	5602.0
13	5452.0
14	5302.0
15	5067.5
16	4833.0
17	4734.0
18	4635.0
19	4433.5
20	4232.0
21	4967.5
22	5703.0
23	5712.5
24	5722.0
25	6101.5
26	8320.0
27	10159.0
28	11074.0
29	11989.0
30	14666.0
31	17343.0
32	21997.5
33	26652.0
34	33120.0
35	39588.0
36	41807.5
37	44027.0
38	49604.5
39	55182.0
40	74661.0
41	94140.0
42	115330.5
43	136521.0
44	138635.0
45	140749.0
46	136058.0
47	131367.0
48	121293.5
49	111220.0
50	104144.0
51	97068.0
52	89439.0
53	81810.0
54	72964.5
55	64119.0
56	57319.0
57	50519.0
58	49745.5
59	48972.0
60	45891.5
61	42811.0
62	36467.5
63	30124.0
64	25746.0
65	21368.0
66	16685.0
67	12002.0
68	9943.0
69	7884.0
70	7254.0
71	6624.0
72	5789.5
73	4955.0
74	3917.0
75	2197.0
76	1515.0
77	1125.5
78	736.0
79	605.5
80	475.0
81	311.0
82	147.0
83	91.5
84	36.0
85	24.0
86	12.0
87	8.5
88	5.0
89	6.0
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1356268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.821397635890946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.04627588728845	15.25404185382776
2	7.301456597489926	2.748472358851145
3	2.4869624274457323	1.4042432625743015
4	1.2228238044203128	0.920610122465106
5	0.7399209167723075	0.6963172896842286
6	0.47915390536180014	0.54110077089627
7	0.344400482734958	0.4537468902083207
8	0.2570641581468117	0.3870645390733359
9	0.22424405023680166	0.3798527793290594
>10	2.911256803065076	13.761968884724643
>50	1.4926264430321665	20.645419256703494
>100	1.4874780891212231	41.337201044985335
>500	0.005148353343603807	0.6146391139546127
>1k	7.92054360554432E-4	0.44007146844385553
>5k	3.96027180277216E-4	0.4152503642785771
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5571	0.41075952540353383	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3021	0.2227435875505431	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2883	0.21256860738438124	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.373174033450616E-5	0.0	0.0	0.08361179353932999	0.0
2	7.373174033450616E-5	0.0	0.0	0.2740608788233594	0.0
3	7.373174033450616E-5	0.0	0.0	0.38001338968404474	0.0
4	7.373174033450616E-5	0.0	0.0	0.604379075521947	0.0
5	7.373174033450616E-5	0.0	0.0	0.981222000371608	0.0
6	7.373174033450616E-5	0.0	0.0	1.3457517245854065	0.0
7	7.373174033450616E-5	0.0	0.0	1.556771965422763	0.0
8	7.373174033450616E-5	0.0	0.0	2.0327103492820005	0.0
9	7.373174033450616E-5	0.0	0.0	2.179510244288002	0.0
10	7.373174033450616E-5	0.0	0.0	2.4344746023647246	0.0
11	1.4746348066901232E-4	0.0	0.0	2.6957798901102143	0.0
12	1.4746348066901232E-4	0.0	0.0	2.925306797771532	0.0
13	1.4746348066901232E-4	0.0	0.0	3.015333252719964	0.0
14	1.4746348066901232E-4	0.0	0.0	3.050871951561196	0.0
15	1.4746348066901232E-4	0.0	0.0	3.1080140503204383	0.0
16	1.4746348066901232E-4	0.0	0.0	3.2212660034742395	0.0
17	1.4746348066901232E-4	0.0	0.0	3.3530983551923366	0.0
18	1.4746348066901232E-4	0.0	0.0	3.5490035892611194	0.0
19	1.4746348066901232E-4	0.0	0.0	3.626126989651013	0.0
20	1.4746348066901232E-4	0.0	0.0	3.709296392748336	0.0
21	1.4746348066901232E-4	0.0	0.0	3.8260874694381934	0.0
22	1.4746348066901232E-4	0.0	0.0	3.9446481078960796	0.0
23	1.4746348066901232E-4	0.0	0.0	4.090268295056729	0.0
24	2.2119522100351848E-4	0.0	0.0	4.194746171110724	0.0
25	2.2119522100351848E-4	0.0	0.0	4.276219744180354	0.0
26	2.2119522100351848E-4	0.0	0.0	4.355702560260951	0.0
27	2.2119522100351848E-4	0.0	0.0	4.435996425485229	0.0
28	2.2119522100351848E-4	0.0	0.0	4.51864970640021	0.0
29	2.2119522100351848E-4	0.0	0.0	4.606759136099945	0.0
30	2.2119522100351848E-4	0.0	0.0	4.728195312430876	0.0
31	2.2119522100351848E-4	0.0	0.0	4.8311248219378475	0.0
32	2.9492696133802464E-4	0.0	0.0	4.921372472107283	0.0
33	3.686587016725308E-4	0.0	0.0	5.008965779624676	0.0
34	3.686587016725308E-4	0.0	0.0	5.105628091203213	0.0
35	3.686587016725308E-4	0.0	0.0	5.236133271595289	0.0
36	3.686587016725308E-4	0.0	0.0	5.343781612483668	0.0
37	3.686587016725308E-4	0.0	0.0	5.455485199090445	0.0
38	3.686587016725308E-4	0.0	0.0	5.555465438984036	0.0
39	3.686587016725308E-4	0.0	0.0	5.659205997634686	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAAAG	20	7.033736E-4	45.0	19
TCGTTGC	20	7.033736E-4	45.0	43
ACACGGT	25	3.8911792E-5	45.0	13
CTATCGA	20	7.033736E-4	45.0	24
GAATCGG	20	7.033736E-4	45.0	22
GCAAACG	25	3.8911792E-5	45.0	10
TATGCGT	20	7.033736E-4	45.0	20
ACGATAT	20	7.033736E-4	45.0	14
ACTAGCG	20	7.033736E-4	45.0	21
ACCTACG	20	7.033736E-4	45.0	39
TCCGTAC	20	7.033736E-4	45.0	13
GTTCACG	25	3.8911792E-5	45.0	1
TTACGTC	25	3.8911792E-5	45.0	16
CCGAACT	45	3.8562575E-10	45.0	42
GGACCGT	40	6.8157533E-9	45.0	8
GTGACGT	20	7.033736E-4	45.0	14
CGCCGAA	20	7.033736E-4	45.0	28
GCGTCTA	25	3.8911792E-5	45.0	9
TATCGTG	35	1.2121382E-7	45.0	1
TACGTCC	25	3.8911792E-5	45.0	17
>>END_MODULE