##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546269_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2173630
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210236792830425	31.0	31.0	33.0	30.0	34.0
2	31.442624549716374	31.0	31.0	34.0	30.0	34.0
3	31.520955728435844	31.0	31.0	34.0	30.0	34.0
4	35.29782253649425	37.0	35.0	37.0	33.0	37.0
5	35.024134282283555	37.0	35.0	37.0	32.0	37.0
6	35.16268270128771	37.0	35.0	37.0	32.0	37.0
7	35.69599241821285	37.0	35.0	37.0	35.0	37.0
8	35.76381168828181	37.0	35.0	37.0	35.0	37.0
9	37.46254146289847	39.0	37.0	39.0	35.0	39.0
10	36.798910118097375	39.0	37.0	39.0	32.0	39.0
11	36.48980645280015	39.0	35.0	39.0	32.0	39.0
12	35.584595354315134	37.0	35.0	39.0	31.0	39.0
13	35.08600037724912	37.0	35.0	39.0	30.0	39.0
14	36.04661372910753	38.0	35.0	40.0	30.0	41.0
15	36.344868721907595	38.0	35.0	40.0	31.0	41.0
16	36.487883862478895	38.0	35.0	40.0	31.0	41.0
17	36.41526432741543	38.0	35.0	40.0	31.0	41.0
18	36.386763156562985	38.0	35.0	40.0	31.0	41.0
19	36.31947939621739	37.0	35.0	40.0	31.0	41.0
20	36.21952862262667	37.0	35.0	40.0	31.0	41.0
21	36.06427634878061	37.0	34.0	40.0	30.0	41.0
22	35.955815847223306	37.0	34.0	40.0	30.0	41.0
23	35.94121216582399	37.0	34.0	40.0	30.0	41.0
24	35.81721544145048	37.0	34.0	40.0	30.0	41.0
25	35.682522784466535	36.0	34.0	40.0	30.0	41.0
26	35.63829952659837	36.0	34.0	40.0	30.0	41.0
27	35.554400242911626	36.0	34.0	40.0	29.0	41.0
28	35.51795107723026	36.0	34.0	40.0	29.0	41.0
29	35.5636184631239	36.0	34.0	40.0	30.0	41.0
30	35.47561452501115	36.0	34.0	40.0	29.0	41.0
31	35.359945804943806	36.0	34.0	40.0	29.0	41.0
32	35.18911038217176	36.0	34.0	40.0	29.0	41.0
33	35.020800228189714	36.0	34.0	40.0	27.0	41.0
34	34.87858145130496	36.0	34.0	40.0	27.0	41.0
35	34.718204570235045	36.0	34.0	40.0	26.0	41.0
36	34.51806517208541	36.0	33.0	40.0	25.0	41.0
37	34.41489995997479	36.0	33.0	40.0	24.0	41.0
38	34.40400436136785	35.0	33.0	40.0	25.0	41.0
39	34.34483007687601	35.0	33.0	40.0	24.0	41.0
40	34.163326325087525	35.0	33.0	40.0	23.0	41.0
41	34.122971250856864	35.0	33.0	40.0	23.0	41.0
42	34.079871459264	35.0	33.0	40.0	23.0	41.0
43	34.01202320542134	35.0	33.0	40.0	23.0	41.0
44	33.78592906796465	35.0	33.0	40.0	23.0	41.0
45	33.78248275925525	35.0	33.0	39.0	23.0	41.0
46	33.73691520635987	35.0	33.0	39.0	23.0	41.0
47	33.71024645408832	35.0	33.0	39.0	23.0	41.0
48	33.623523322736624	35.0	33.0	39.0	23.0	41.0
49	33.54045490722892	35.0	32.0	39.0	24.0	41.0
50	33.45817825480878	35.0	32.0	39.0	23.0	41.0
51	33.367798107313575	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	0.0
12	1.0
13	9.0
14	24.0
15	75.0
16	283.0
17	712.0
18	1462.0
19	2886.0
20	4650.0
21	7486.0
22	10994.0
23	15674.0
24	21454.0
25	28892.0
26	36979.0
27	43688.0
28	49418.0
29	57001.0
30	68329.0
31	83096.0
32	103286.0
33	132678.0
34	222962.0
35	309119.0
36	156142.0
37	188834.0
38	258180.0
39	369093.0
40	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.24917304233011	25.208568155573857	27.235776098047964	14.306482704048069
2	29.456347216407575	27.59655507147031	30.196675607164053	12.750422104958064
3	28.86995486812383	26.6153393171791	31.30233756435088	13.212368250346195
4	26.6999903387421	28.935467397855202	29.911484475278684	14.453057788124013
5	24.215436849877854	33.07821478356482	28.86917276629417	13.837175600263155
6	21.94683547797923	41.07262965638126	26.025312495686936	10.955222369952567
7	85.52775771405436	5.789669815009915	6.67537713410286	2.0071953368328557
8	87.15494357365328	4.779608305001312	6.105684960181815	1.9597631611635837
9	82.08600359766841	6.839020440461348	7.92434775007706	3.1506282117931756
10	50.67730018448402	23.91980235826705	13.633047022722359	11.769850434526576
11	46.40159548773251	20.894310439219186	18.8821464554685	13.821947617579807
12	40.69685273022547	22.576243426894184	22.74770775154925	13.979196091331092
13	22.639823705046396	40.131577131342496	22.328869218772283	14.89972994483882
14	16.567171045670147	41.78130592603157	26.57434798010701	15.077175048191274
15	16.11727846965675	23.582486439734453	44.79373214392514	15.506502946683659
16	17.040020610683513	19.62633934938329	42.652889406200686	20.68075063373251
17	17.156139729392766	20.31587712720196	28.198175402437396	34.32980774096787
18	22.085911585688457	24.400150899647134	32.20796547710512	21.30597203755929
19	29.732107120347067	25.3299779631308	23.846238780289195	21.091676136232937
20	30.69850894586475	23.818589180311275	24.86513344037394	20.617768433450035
21	22.199960434848617	28.189112222411357	27.63796046245221	21.972966880287814
22	22.59119537363765	25.764688562450832	24.560021714827272	27.084094349084253
23	20.77837534446985	29.65256276367183	24.500996029683066	25.068065862175253
24	20.95154189075418	24.396148378518884	35.929390006578856	18.722919724148085
25	18.500434756605312	26.404447859111258	33.12155242612588	21.973564958157553
26	17.62204238991917	34.23218303023054	26.066763892658823	22.079010687191474
27	19.28819532303106	32.83824753982969	27.807814577457986	20.065742559681272
28	16.091377097298068	30.293104162161917	35.34304366428509	18.272475076254928
29	16.92629380345321	25.84887952411404	34.81977153425376	22.40505513817899
30	19.079926206392074	31.20917543464159	30.247006160202055	19.46389219876428
31	28.472509120687512	28.18957228231116	23.794344023591872	19.543574573409458
32	28.98943242410162	28.164176975842253	24.726149344644675	18.120241255411454
33	27.350791072997705	28.732995035953678	23.72924554777032	20.186968343278295
34	20.48025652940013	28.853944783610824	27.47914778504161	23.186650901947434
35	22.353988489301308	26.591370196399573	28.67189908126038	22.38274223303874
36	29.14290840667455	26.79692495962974	24.892322980452057	19.16784365324365
37	21.526984813422708	32.754792674006154	27.27800959684951	18.440212915721627
38	21.985204473622467	32.72120830132083	23.414334546357935	21.879252678698766
39	22.187630829534005	30.722064012734457	25.841932619627077	21.248372538104462
40	23.90508044147348	27.07585927687785	25.04460280728551	23.974457474363163
41	18.48299848640293	25.811062600350564	26.392486301716485	29.31345261153002
42	22.701379719639498	26.938899444707705	23.454819817540244	26.90490101811256
43	22.966098185983817	26.61515529321918	25.34796630521294	25.070780215584072
44	20.709918431379766	30.215768093005707	27.245989427823503	21.82832404779102
45	18.718641167079955	36.47046645473241	22.32224435621518	22.48864802197246
46	22.421985342491592	32.73188169099617	25.257242492972587	19.588890473539657
47	22.98749097132447	28.275419459613644	26.346848359656427	22.390241209405463
48	23.88617197959174	25.183954950934613	28.94857910499947	21.981293964474176
49	21.904878015117568	25.223428090337364	30.18071152864103	22.69098236590404
50	20.420448742426263	32.49297258503057	26.332954550682498	20.753624121860668
51	19.670367081794048	32.809493796092255	24.22160165253516	23.29853746957854
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2270.0
1	2827.0
2	3384.0
3	8389.0
4	13394.0
5	10342.5
6	7291.0
7	7673.5
8	8056.0
9	8607.5
10	9159.0
11	9503.0
12	9847.0
13	9710.0
14	9573.0
15	9229.0
16	8885.0
17	8604.0
18	8323.0
19	8200.5
20	8078.0
21	8485.0
22	8892.0
23	8520.0
24	8148.0
25	10513.5
26	14616.5
27	16354.0
28	22134.0
29	27914.0
30	31817.5
31	35721.0
32	41400.0
33	47079.0
34	51637.0
35	56195.0
36	60940.0
37	65685.0
38	73218.5
39	80752.0
40	113177.0
41	145602.0
42	170633.5
43	195665.0
44	207400.5
45	219136.0
46	203095.5
47	187055.0
48	181980.5
49	176906.0
50	160832.5
51	144759.0
52	134873.0
53	124987.0
54	113133.5
55	101280.0
56	98760.0
57	96240.0
58	90436.5
59	84633.0
60	78395.0
61	72157.0
62	62453.0
63	52749.0
64	45997.5
65	39246.0
66	31904.0
67	24562.0
68	22438.0
69	20314.0
70	17338.5
71	14363.0
72	13081.5
73	11800.0
74	9295.5
75	5370.5
76	3950.0
77	2814.0
78	1678.0
79	1259.0
80	840.0
81	754.5
82	669.0
83	447.0
84	225.0
85	156.5
86	88.0
87	56.5
88	25.0
89	21.0
90	17.0
91	14.0
92	11.0
93	7.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2173630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.719590139109577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.83482529043339	14.500795632521182
2	7.384131370745467	2.6168756284590216
3	2.353276622045318	1.250972916797739
4	1.1122495616980328	0.7883442546277363
5	0.656494920302235	0.5816410458081505
6	0.43807184635456736	0.46574721413315506
7	0.3396384661257945	0.4212778090657451
8	0.2514884717544405	0.35650181153597765
9	0.2026621445089905	0.3231981123671077
>10	2.503120449238076	10.871946678601667
>50	1.2369044588163802	16.058354275134732
>100	1.6755716680337644	49.22577679699986
>500	0.00893638222908855	0.9872596314610661
>1k	0.0021026781715502473	0.8472138860226556
>5k	5.256695428875618E-4	0.7040943064642134
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7838	0.36059494946242	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7280	0.3349236070536384	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3960	0.1821837203203857	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2934	0.13498157460101304	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2849	0.13107106545272193	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2196	0.10102915399585026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06749078730050653	0.0
2	0.0	0.0	0.0	0.1832418580899233	0.0
3	0.0	0.0	0.0	0.2579095798272935	0.0
4	0.0	0.0	0.0	0.38507013613172436	0.0
5	0.0	0.0	0.0	0.5988599715682982	0.0
6	0.0	0.0	0.0	0.8028965371291342	0.0
7	0.0	0.0	0.0	0.9213619613273648	0.0
8	0.0	0.0	0.0	1.1843322000524468	0.0
9	0.0	0.0	0.0	1.28848976136693	0.0
10	4.600598997989538E-5	0.0	0.0	1.4816229073025309	0.0
11	4.600598997989538E-5	0.0	0.0	1.6910421736910146	0.0
12	9.201197995979076E-5	0.0	0.0	1.8880858287749065	0.0
13	9.201197995979076E-5	0.0	0.0	1.967860215400045	0.0
14	9.201197995979076E-5	0.0	0.0	2.0036988815943837	0.0
15	9.201197995979076E-5	0.0	0.0	2.0564217461113437	0.0
16	9.201197995979076E-5	0.0	0.0	2.17672740990877	0.0
17	9.201197995979076E-5	0.0	0.0	2.312215050399562	0.0
18	9.201197995979076E-5	0.0	0.0	2.506084292174841	0.0
19	9.201197995979076E-5	0.0	0.0	2.5866867866196177	0.0
20	9.201197995979076E-5	0.0	0.0	2.6752023113409367	0.0
21	9.201197995979076E-5	0.0	0.0	2.7846505615031076	0.0
22	9.201197995979076E-5	0.0	0.0	2.8942828356251984	0.0
23	9.201197995979076E-5	0.0	0.0	3.0260899969175985	0.0
24	9.201197995979076E-5	0.0	0.0	3.1237147076549365	0.0
25	9.201197995979076E-5	0.0	0.0	3.2042711961097337	0.0
26	9.201197995979076E-5	0.0	0.0	3.280089067596601	0.0
27	9.201197995979076E-5	0.0	0.0	3.355906939083469	0.0
28	9.201197995979076E-5	0.0	0.0	3.4439163979150087	0.0
29	9.201197995979076E-5	0.0	0.0	3.5282913835381366	0.0
30	9.201197995979076E-5	0.0	0.0	3.642616268638177	0.0
31	1.3801796993968615E-4	0.0	0.0	3.7543188123093625	0.0
32	1.3801796993968615E-4	0.0	0.0	3.854381840515635	0.0
33	1.3801796993968615E-4	0.0	0.0	3.955088952581626	0.0
34	1.8402395991958152E-4	0.0	0.0	4.055612040687698	0.0
35	1.8402395991958152E-4	0.0	0.0	4.179644189673495	0.0
36	2.3002994989947692E-4	0.0	0.0	4.2882183260260485	0.0
37	2.3002994989947692E-4	0.0	0.0	4.394722192829507	0.0
38	2.3002994989947692E-4	0.0	0.0	4.500213927853406	0.0
39	2.3002994989947692E-4	0.0	0.0	4.601841159718996	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATACG	105	0.0	45.000004	1
CGTAGGT	30	2.1663982E-6	45.000004	3
CGCATAC	20	7.034677E-4	45.0	36
CGACGGT	300	0.0	43.500004	28
CTATGCG	215	0.0	42.906975	1
CTAAGCG	100	0.0	42.75	1
CGTTTTT	6055	0.0	42.65896	1
CTCACGA	325	0.0	42.23077	24
CGGTCTA	310	0.0	42.09677	31
ATACCGG	75	0.0	42.000004	2
TATTACG	60	3.6379788E-12	41.250004	1
CGCATCG	55	6.184564E-11	40.909092	21
TCGTACA	110	0.0	40.909092	34
AGGGCGC	655	0.0	40.190838	6
CACGGTA	45	1.929584E-8	40.0	13
ACGTTAT	45	1.929584E-8	40.0	28
CGACATA	45	1.929584E-8	40.0	36
TCGACAC	45	1.929584E-8	40.0	34
AGTACGT	45	1.929584E-8	40.0	25
TACTCGC	220	0.0	39.886364	45
>>END_MODULE