FastQCFastQC Report
Thu 26 May 2016
SRR1546268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546268_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1890272
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC58060.30715156337289024No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53670.2839273924599211No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG29690.15706734268930608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGT207.034441E-445.00000415
ATTACGC207.034441E-445.00000411
CGTTCGA207.034441E-445.00000414
TACGACC207.034441E-445.00000420
CGCACAT207.034441E-445.00000435
CCACGAA207.034441E-445.00000425
GCGTATC207.034441E-445.00000413
TACGTCA406.8193913E-945.00000429
CTATGCG351.2124838E-745.01
CGTATAA351.2124838E-745.030
TAGGTTA253.8917646E-545.020
TCTAGCG453.8562575E-1045.01
CATAGGT351.2124838E-745.026
TATTACG351.2124838E-745.01
CGTACTT253.8917646E-545.013
ACGAGTT253.8917646E-545.034
ATCGATT502.1827873E-1145.042
GTACGAT453.8562575E-1045.09
TTGATCG302.1662472E-644.99999617
CGCATCG600.044.99999621