##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546268_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1890272
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218107235360836	31.0	31.0	33.0	30.0	34.0
2	31.457875903573665	31.0	31.0	34.0	30.0	34.0
3	31.521948693098135	31.0	31.0	34.0	30.0	34.0
4	35.29371116960945	37.0	35.0	37.0	33.0	37.0
5	35.014115428890655	37.0	35.0	37.0	32.0	37.0
6	35.15398630461648	37.0	35.0	37.0	32.0	37.0
7	35.70229575426182	37.0	35.0	37.0	35.0	37.0
8	35.78507325929813	37.0	35.0	37.0	35.0	37.0
9	37.51606012256437	39.0	37.0	39.0	35.0	39.0
10	36.82434379814122	39.0	37.0	39.0	32.0	39.0
11	36.49587466777268	39.0	35.0	39.0	32.0	39.0
12	35.397056614074586	37.0	35.0	39.0	30.0	39.0
13	34.81221273975385	37.0	34.0	39.0	27.0	39.0
14	35.7111447453065	37.0	34.0	40.0	27.0	41.0
15	36.07771527060656	37.0	34.0	40.0	30.0	41.0
16	36.27930689339947	37.0	35.0	40.0	31.0	41.0
17	36.22963203179225	37.0	35.0	40.0	31.0	41.0
18	36.19776518934841	37.0	35.0	40.0	31.0	41.0
19	36.12136242826429	37.0	35.0	40.0	31.0	41.0
20	36.01490949450661	36.0	34.0	40.0	30.0	41.0
21	35.85179275786765	36.0	34.0	40.0	30.0	41.0
22	35.73252579522947	36.0	34.0	40.0	30.0	41.0
23	35.765033815239285	36.0	34.0	40.0	30.0	41.0
24	35.65343664827073	36.0	34.0	40.0	30.0	41.0
25	35.50323974539114	35.0	34.0	40.0	30.0	41.0
26	35.44154862369014	35.0	34.0	40.0	30.0	41.0
27	35.34965444126559	35.0	34.0	40.0	29.0	41.0
28	35.34792347344721	36.0	34.0	40.0	29.0	41.0
29	35.3973756157844	36.0	34.0	40.0	29.0	41.0
30	35.316715266374366	36.0	34.0	40.0	29.0	41.0
31	35.183640238018654	36.0	34.0	40.0	29.0	41.0
32	35.013021935467485	35.0	34.0	40.0	29.0	41.0
33	34.85954349426961	35.0	34.0	40.0	27.0	41.0
34	34.748372191938515	35.0	34.0	40.0	27.0	41.0
35	34.57823583061062	35.0	33.0	40.0	26.0	41.0
36	34.390384558412755	35.0	33.0	40.0	25.0	41.0
37	34.29207013593811	35.0	33.0	40.0	24.0	41.0
38	34.2830174705016	35.0	33.0	40.0	25.0	41.0
39	34.25779834859745	35.0	33.0	40.0	24.0	41.0
40	34.06636187807892	35.0	33.0	40.0	23.0	41.0
41	33.992009615547396	35.0	33.0	40.0	23.0	41.0
42	33.952181484992636	35.0	33.0	39.0	23.0	41.0
43	33.879733710280846	35.0	33.0	39.0	23.0	41.0
44	33.67120922279968	35.0	33.0	39.0	23.0	41.0
45	33.671849342316875	35.0	33.0	39.0	23.0	41.0
46	33.62093550557803	35.0	33.0	39.0	23.0	41.0
47	33.56954290176228	35.0	33.0	39.0	23.0	41.0
48	33.462306482876535	35.0	32.0	39.0	23.0	41.0
49	33.41512068104485	35.0	32.0	39.0	24.0	41.0
50	33.32964250647526	35.0	32.0	39.0	24.0	40.0
51	33.245321308256166	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	4.0
13	7.0
14	29.0
15	73.0
16	211.0
17	604.0
18	1276.0
19	2518.0
20	4264.0
21	6678.0
22	9829.0
23	14130.0
24	19128.0
25	25472.0
26	32828.0
27	38576.0
28	43825.0
29	50881.0
30	60730.0
31	73818.0
32	91986.0
33	119653.0
34	206364.0
35	292929.0
36	128047.0
37	155597.0
38	209698.0
39	300951.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.601128303228315	24.395113507474058	29.264254033281983	13.739504156015641
2	30.55544387262785	26.457303499178952	29.84628667197102	13.140965956222173
3	29.615790743342757	25.578593980125614	30.997073437050332	13.808541839481304
4	27.32289321325185	28.06807697516548	30.08852694215436	14.520502869428315
5	24.155095139747086	32.41321883834708	28.8813461766349	14.550339845270946
6	22.126604001963738	41.205181053308735	25.943726617121772	10.724488327605762
7	86.39423321088182	5.591682043642397	6.037649608098729	1.9764351373770548
8	88.48112864180393	3.974137055407899	5.4976215063229	2.047112796465271
9	84.25258375514211	5.940097509776371	6.95090442010462	2.856414314976892
10	54.90601352609572	22.308535491188568	11.872471263394898	10.912979719320818
11	50.57938751671717	20.263009767906418	16.914391156405003	12.243211558971408
12	44.96590966802661	21.130874286875116	19.96072522896176	13.942490816136512
13	22.390745882074114	40.78614083052598	21.329470044522694	15.493643242877214
14	15.186544581943762	44.114709417480654	26.082066496250274	14.616679504325303
15	14.788929847133112	23.01996749674121	47.67641905503547	14.514683601090214
16	16.508946860557632	17.882611602986238	46.40406248412926	19.20437905232686
17	16.444882006399077	18.73947241455198	27.96407077923177	36.85157479981717
18	21.769829950398673	23.671090721335343	32.7106363528635	21.84844297540248
19	32.009520323001134	24.75548492492086	23.574914086438355	19.660080665639654
20	34.16032190076349	23.667652062771918	22.931937837517562	19.24008819894703
21	24.412888727124983	28.161978805166665	26.306901863858744	21.118230603849604
22	22.802432665775086	25.381162076145657	24.515995581588257	27.300409676491
23	21.130874286875116	30.134816576661983	23.35817279206379	25.376136344399114
24	21.049457432581132	24.123142066326963	36.927383995530796	17.900016505561105
25	19.192634710771785	25.2305488310677	34.080809534289244	21.496006923871274
26	17.579745137207766	35.91218618272926	25.117760830187404	21.390307849875573
27	19.684468690220243	34.028965143640704	27.596187215384877	18.690378950754177
28	16.89164310744697	29.479831473988927	36.583253626991244	17.04527179157285
29	16.682308154593624	26.257808400060945	35.52361776506238	21.536265680283048
30	19.40487929779418	30.87433977755582	30.35462621252391	19.366154712126086
31	30.237129894533698	26.92051725889184	23.316274060029457	19.526078786545007
32	32.430941155558564	26.101587496402633	24.97577068273772	16.491700665301078
33	28.980591153019247	28.065061536117554	23.014042423524234	19.940304887338964
34	21.15161204313453	29.009211372754823	25.919232787662306	23.919943796448344
35	22.34011824753263	25.735343908178294	28.942289786866652	22.982248057422424
36	31.434365001438948	25.600495590052645	24.224344432970494	18.74079497553791
37	21.799614023801865	33.16623216129742	26.80238611162838	18.231767703272332
38	21.552771241387482	33.59881540857612	22.01328697668907	22.83512637334733
39	22.487081224289415	31.189585414162618	24.992487853599904	21.330845507948062
40	25.12463814731425	25.385552978618954	24.76908085185624	24.72072802221056
41	18.343286045606135	24.886577169846454	26.711922940190618	30.05821384435679
42	23.151535863621746	27.057481674594978	22.68858661610604	27.102395845677236
43	23.752137258553265	26.30727218093481	24.71628421729783	25.2243063432141
44	20.524771038242115	31.073411657158335	26.2812970831711	22.12052022142845
45	18.123635117062516	37.67801670870647	21.942556415161416	22.25579175906959
46	22.832428348936027	33.280184015845336	24.045005163278088	19.842382471940546
47	23.817101454182254	27.386005823500533	25.46321375971289	23.333678962604324
48	24.078016285486957	24.189111408305262	30.41477628616411	21.318096020043676
49	22.601720810550017	24.141657920130015	29.945372941037057	23.311248328282915
50	20.1526023768008	33.47301340759425	24.724960217365542	21.649423998239406
51	19.535654128083156	34.527253220700516	22.848828105161587	23.08826454605475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1209.0
1	1995.0
2	2781.0
3	6468.0
4	10155.0
5	7880.0
6	5605.0
7	6040.0
8	6475.0
9	6945.5
10	7416.0
11	7667.0
12	7918.0
13	7781.5
14	7645.0
15	7304.0
16	6963.0
17	6763.5
18	6564.0
19	7028.5
20	7493.0
21	7322.0
22	7151.0
23	6795.5
24	6440.0
25	9275.0
26	13204.0
27	14298.0
28	18270.0
29	22242.0
30	23286.5
31	24331.0
32	29117.5
33	33904.0
34	37769.5
35	41635.0
36	43278.0
37	44921.0
38	57728.5
39	70536.0
40	100079.5
41	129623.0
42	155898.5
43	182174.0
44	185051.5
45	187929.0
46	181945.0
47	175961.0
48	156458.5
49	136956.0
50	129697.0
51	122438.0
52	113414.0
53	104390.0
54	99876.5
55	95363.0
56	88875.0
57	82387.0
58	81825.0
59	81263.0
60	75521.0
61	69779.0
62	62387.5
63	54996.0
64	45935.0
65	36874.0
66	31180.0
67	25486.0
68	21055.0
69	16624.0
70	15372.5
71	14121.0
72	12438.5
73	10756.0
74	9448.5
75	5806.5
76	3472.0
77	2653.0
78	1834.0
79	1587.0
80	1340.0
81	822.0
82	304.0
83	196.0
84	88.0
85	83.5
86	79.0
87	74.5
88	70.0
89	42.0
90	14.0
91	11.0
92	8.0
93	8.0
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1890272.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.504571080948217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.6446229077022	13.47509482157384
2	7.389466894077031	2.4391996320721603
3	2.372622057628167	1.1747732819504908
4	1.1437904498196134	0.7551108312303018
5	0.6751671987963309	0.5571672512029369
6	0.47074390017800855	0.46616556968464395
7	0.3019270550017424	0.34882235783763343
8	0.2479857906017578	0.32743192864414816
9	0.1888475494562746	0.28051630231175717
>10	2.367922768642823	9.702566896901226
>50	1.3094064026970542	15.812787450691287
>100	1.8757855650552675	52.53919183447668
>500	0.008783595257802874	0.8887702498626332
>1k	0.002277228400171116	0.6324964383121984
>5k	6.50636685763176E-4	0.5999051532480638
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5806	0.30715156337289024	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5367	0.2839273924599211	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2969	0.15706734268930608	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03475690271029778	0.0
2	0.0	0.0	0.0	0.12410912291987608	0.0
3	0.0	0.0	0.0	0.1862694892586887	0.0
4	0.0	0.0	0.0	0.31455790489411045	0.0
5	0.0	0.0	0.0	0.5219883699277141	0.0
6	0.0	0.0	0.0	0.7398935179699007	0.0
7	0.0	0.0	0.0	0.854850518867126	0.0
8	0.0	0.0	0.0	1.1039681061773121	0.0
9	0.0	0.0	0.0	1.186284301941731	0.0
10	0.0	0.0	0.0	1.3289092786646577	0.0
11	0.0	0.0	0.0	1.520627719185387	0.0
12	0.0	0.0	0.0	1.6676965008210458	0.0
13	0.0	0.0	0.0	1.7342477696331533	0.0
14	0.0	0.0	0.0	1.761439623503919	0.0
15	0.0	0.0	0.0	1.80159257503682	0.0
16	0.0	0.0	0.0	1.8916854293985204	0.0
17	0.0	0.0	0.0	1.993787137512485	0.0
18	0.0	0.0	0.0	2.1310689678522454	5.290243943728733E-5
19	0.0	0.0	0.0	2.1982550659376003	5.290243943728733E-5
20	0.0	0.0	0.0	2.2609973591102235	5.290243943728733E-5
21	0.0	0.0	0.0	2.348709603697246	5.290243943728733E-5
22	0.0	0.0	0.0	2.4344644580250883	5.290243943728733E-5
23	0.0	0.0	0.0	2.540057727141914	5.290243943728733E-5
24	0.0	0.0	0.0	2.620733947283777	5.290243943728733E-5
25	0.0	0.0	0.0	2.6839523624113357	5.290243943728733E-5
26	0.0	0.0	0.0	2.7524610214826226	5.290243943728733E-5
27	0.0	0.0	0.0	2.8155736317313065	5.290243943728733E-5
28	0.0	0.0	0.0	2.8809081444363565	5.290243943728733E-5
29	0.0	0.0	0.0	2.9578811938176095	5.290243943728733E-5
30	0.0	0.0	0.0	3.0595067799766382	5.290243943728733E-5
31	0.0	0.0	0.0	3.1542550490088197	5.290243943728733E-5
32	0.0	0.0	0.0	3.2431311472634627	5.290243943728733E-5
33	5.290243943728733E-5	0.0	0.0	3.3254473430278817	5.290243943728733E-5
34	5.290243943728733E-5	0.0	0.0	3.412630563220531	5.290243943728733E-5
35	5.290243943728733E-5	0.0	0.0	3.527693368996631	5.290243943728733E-5
36	1.0580487887457466E-4	0.0	0.0	3.626303516107735	5.290243943728733E-5
37	1.0580487887457466E-4	0.0	0.0	3.723379492475157	5.290243943728733E-5
38	1.0580487887457466E-4	0.0	0.0	3.8158000541720978	5.290243943728733E-5
39	1.0580487887457466E-4	0.0	0.0	3.9089083475817237	5.290243943728733E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTAGT	20	7.034441E-4	45.000004	15
ATTACGC	20	7.034441E-4	45.000004	11
CGTTCGA	20	7.034441E-4	45.000004	14
TACGACC	20	7.034441E-4	45.000004	20
CGCACAT	20	7.034441E-4	45.000004	35
CCACGAA	20	7.034441E-4	45.000004	25
GCGTATC	20	7.034441E-4	45.000004	13
TACGTCA	40	6.8193913E-9	45.000004	29
CTATGCG	35	1.2124838E-7	45.0	1
CGTATAA	35	1.2124838E-7	45.0	30
TAGGTTA	25	3.8917646E-5	45.0	20
TCTAGCG	45	3.8562575E-10	45.0	1
CATAGGT	35	1.2124838E-7	45.0	26
TATTACG	35	1.2124838E-7	45.0	1
CGTACTT	25	3.8917646E-5	45.0	13
ACGAGTT	25	3.8917646E-5	45.0	34
ATCGATT	50	2.1827873E-11	45.0	42
GTACGAT	45	3.8562575E-10	45.0	9
TTGATCG	30	2.1662472E-6	44.999996	17
CGCATCG	60	0.0	44.999996	21
>>END_MODULE