FastQCFastQC Report
Thu 26 May 2016
SRR1546267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546267_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2292896
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC74830.32635583995087436No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64680.2820886773756856No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG34350.1498105452667718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA253.892024E-545.00000426
AATGCGC253.892024E-545.00000422
CTACGAT253.892024E-545.00000443
CGGTACC253.892024E-545.00000414
GTGCGTA253.892024E-545.00000429
TCTACGT207.0347585E-445.012
TTACGTC207.0347585E-445.013
GCGTAAT351.2126293E-745.031
CGTCGAT302.166451E-644.99999631
GTACGTC302.166451E-644.99999629
TACGTCA302.166451E-644.99999630
GCGATAT2150.043.953499
CTATACG1100.042.9545441
TATAGCG950.042.631581
TAGTACG1600.042.18751
ACGCTAG700.041.7857131
CGTTTTT62700.041.5909081
GCTAACG650.041.538461
CTAATGC603.6379788E-1241.24999620
CGACGGT2550.040.58823428
Produced by FastQC (version 0.11.5)