##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546267_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2292896
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25444459757442	31.0	31.0	33.0	30.0	34.0
2	31.505962764992393	31.0	31.0	34.0	30.0	34.0
3	31.57860844975088	31.0	31.0	34.0	30.0	34.0
4	35.33783041184598	37.0	35.0	37.0	33.0	37.0
5	35.06778283882043	37.0	35.0	37.0	32.0	37.0
6	35.200145580087366	37.0	35.0	37.0	32.0	37.0
7	35.7298996552831	37.0	35.0	37.0	35.0	37.0
8	35.80131196530501	37.0	35.0	37.0	35.0	37.0
9	37.53942263408371	39.0	37.0	39.0	35.0	39.0
10	36.86851039035351	39.0	37.0	39.0	32.0	39.0
11	36.5111810566201	39.0	35.0	39.0	32.0	39.0
12	35.48339828758042	37.0	35.0	39.0	31.0	39.0
13	34.926796069250415	37.0	34.0	39.0	29.0	39.0
14	35.8592103610456	38.0	34.0	40.0	29.0	41.0
15	36.19293548420862	38.0	35.0	40.0	30.0	41.0
16	36.38375835624468	38.0	35.0	40.0	31.0	41.0
17	36.3251538665513	37.0	35.0	40.0	31.0	41.0
18	36.29364567778041	37.0	35.0	40.0	31.0	41.0
19	36.19980321828814	37.0	35.0	40.0	31.0	41.0
20	36.13156898524835	37.0	35.0	40.0	31.0	41.0
21	35.95756937950958	37.0	34.0	40.0	30.0	41.0
22	35.82715395726627	36.0	34.0	40.0	30.0	41.0
23	35.84395672546858	36.0	34.0	40.0	30.0	41.0
24	35.73063409766514	36.0	34.0	40.0	30.0	41.0
25	35.58266576416898	36.0	34.0	40.0	30.0	41.0
26	35.50999042259222	36.0	34.0	40.0	30.0	41.0
27	35.432747058741434	36.0	34.0	40.0	29.0	41.0
28	35.42882363613526	36.0	34.0	40.0	29.0	41.0
29	35.48327966030732	36.0	34.0	40.0	30.0	41.0
30	35.41603980293917	36.0	34.0	40.0	29.0	41.0
31	35.276804094036535	36.0	34.0	40.0	29.0	41.0
32	35.10158725035937	35.0	34.0	40.0	29.0	41.0
33	34.946428882949775	36.0	34.0	40.0	27.0	41.0
34	34.808493712754526	35.0	34.0	40.0	27.0	41.0
35	34.63636423108593	35.0	33.0	40.0	26.0	41.0
36	34.447103139435896	35.0	33.0	40.0	25.0	41.0
37	34.337426555761795	35.0	33.0	40.0	24.0	41.0
38	34.328146152289506	35.0	33.0	40.0	25.0	41.0
39	34.29065731720933	35.0	33.0	40.0	24.0	41.0
40	34.12098106499379	35.0	33.0	40.0	23.0	41.0
41	34.07096833000712	35.0	33.0	40.0	23.0	41.0
42	34.03869953107337	35.0	33.0	40.0	24.0	41.0
43	33.96449075753981	35.0	33.0	40.0	24.0	41.0
44	33.75154389906913	35.0	33.0	39.0	23.0	41.0
45	33.72598103010341	35.0	33.0	39.0	23.0	41.0
46	33.692013942193626	35.0	33.0	39.0	23.0	41.0
47	33.65552209956317	35.0	33.0	39.0	23.0	41.0
48	33.57251135681688	35.0	33.0	39.0	23.0	41.0
49	33.505164647677	35.0	32.0	39.0	24.0	41.0
50	33.41716676203369	35.0	32.0	39.0	24.0	40.0
51	33.32502389990649	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	3.0
13	2.0
14	27.0
15	88.0
16	280.0
17	648.0
18	1561.0
19	2848.0
20	4723.0
21	7691.0
22	11424.0
23	15943.0
24	22314.0
25	31113.0
26	39582.0
27	46598.0
28	52094.0
29	60315.0
30	72063.0
31	88164.0
32	108652.0
33	141430.0
34	243850.0
35	353619.0
36	159093.0
37	193327.0
38	263349.0
39	371886.0
40	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.711030940784056	25.122683279136954	29.6553354360599	14.510950344019092
2	29.652500593136367	26.726942696049015	30.881644871812764	12.738911839001856
3	28.926562739871326	25.6926175456715	31.056402034806634	14.324417679650539
4	26.28501249075405	27.41044513139715	30.622234937825354	15.682307440023447
5	23.622397178066514	32.494059913750995	29.72851799645514	14.15502491172735
6	22.45095285612605	40.36903549048888	26.25278250736187	10.927229146023196
7	86.49349992324117	5.280658172023502	6.19155862280714	2.034283281928182
8	88.66128250038379	3.998044394512442	5.702526412013454	1.6381466930903101
9	84.73118711010007	5.284147209467852	7.054441195762913	2.9302244846691696
10	55.230546871729025	20.883023041603284	12.277966379635185	11.608463707032504
11	49.42823398880717	19.730157844053984	17.907353844221458	12.934254322917393
12	42.81489435194619	21.690866048874437	21.962487613917073	13.531751985262305
13	21.956338185421405	40.576066249842995	22.753757693327564	14.713837871408037
14	15.637604147767714	42.83491270428314	27.38170418544932	14.145778962499826
15	15.032866732725775	21.855897519992183	48.511096883591755	14.600138863690285
16	17.04045887820468	16.915246046920576	46.45810363836825	19.586191436506496
17	17.62199419424169	17.65252327187975	28.495535776589954	36.2299467572886
18	23.12721553877716	22.430323922236333	32.56741692601845	21.875043612968053
19	30.83772661298201	23.39286212719635	24.766670620909103	21.002740638912538
20	32.56959757442117	23.156174549565268	23.377859266185645	20.89636860982792
21	23.518162184416564	27.045927944398702	27.326228490084155	22.109681381100582
22	23.32024653538582	24.88787105913221	25.131013356035336	26.66086904944664
23	20.801990146958257	29.59231469722133	24.128438446401407	25.477256709419006
24	21.172656762452373	23.837409110574576	36.52926255704576	18.46067156992729
25	18.549903702566535	23.941862169064798	34.84440637516922	22.66382775319945
26	17.86600875050591	33.53326971655059	26.598546118096937	22.002175414846555
27	19.672327048413884	32.31476700207946	28.523753366921134	19.48915258258552
28	16.66072076535525	28.6819812150224	36.93176663921957	17.725531380402774
29	17.156687438069586	25.648350383096314	35.73214833991599	21.46281383891812
30	20.05276296875218	29.459120692783276	30.77959052656553	19.708525811899015
31	30.209132904414332	25.648612060904636	23.944217269339735	20.198037765341297
32	31.358552677487335	26.05172672463121	25.43813587707423	17.15158472080722
33	29.088017947608613	26.13729536795389	24.35923827334515	20.41544841109235
34	21.053375294823663	27.66553738154718	27.74822756897827	23.532859754650886
35	22.70225077805535	24.75158053396229	29.77910903939821	22.767059648584148
36	31.54591398824892	24.658074330453715	25.11051526104978	18.68549642024758
37	22.11155673872692	31.690447364381114	27.29338792513921	18.904607971752753
38	21.94848785117162	33.103376690438644	22.030044101433297	22.918091356956445
39	22.35413206704534	30.083440330481626	26.437570653008247	21.12485694946478
40	24.341618634251184	25.28392042203397	24.813031205950903	25.561429737763948
41	18.83487083583381	24.09982833935774	26.790574016440345	30.274726808368108
42	23.36516789248182	25.53761705720626	23.969251113002947	27.127963937308973
43	23.48331542294112	26.017883061421017	25.32570164543006	25.173099870207803
44	21.22507955005373	30.16059167096981	27.264777818095542	21.34955096088091
45	19.11490970371094	36.15157425369489	22.12019210640169	22.613323936192483
46	23.381479142534157	31.91588279625417	24.86065656706628	19.841981494145394
47	23.513888113547235	27.069042817467516	26.101358282277086	23.315710786708163
48	25.479350131885614	23.122854241971723	29.24266953232942	22.155126093813237
49	22.46739494508255	23.407472471494565	31.059629394442663	23.065503188980223
50	20.793878134900144	32.67326559948641	25.13951788480594	21.393338380807503
51	20.021797761433575	33.95801641243214	23.078935983140973	22.941249842993315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1202.0
1	2011.0
2	2820.0
3	7535.0
4	12250.0
5	9367.5
6	6485.0
7	7082.0
8	7679.0
9	8484.0
10	9289.0
11	9268.5
12	9248.0
13	9263.0
14	9278.0
15	9070.0
16	8862.0
17	8333.5
18	7805.0
19	7327.5
20	6850.0
21	7253.5
22	7657.0
23	8817.0
24	9977.0
25	10356.5
26	14095.5
27	17455.0
28	22168.5
29	26882.0
30	29989.5
31	33097.0
32	33416.0
33	33735.0
34	40052.5
35	46370.0
36	48645.0
37	50920.0
38	68617.0
39	86314.0
40	119830.0
41	153346.0
42	187909.0
43	222472.0
44	224350.0
45	226228.0
46	216064.0
47	205900.0
48	192686.5
49	179473.0
50	166752.0
51	154031.0
52	144085.5
53	134140.0
54	124248.5
55	114357.0
56	109786.0
57	105215.0
58	100944.5
59	96674.0
60	91337.0
61	86000.0
62	75265.5
63	64531.0
64	55466.0
65	46401.0
66	39640.5
67	32880.0
68	27788.0
69	22696.0
70	19818.5
71	16941.0
72	14539.5
73	12138.0
74	9419.5
75	5339.0
76	3977.0
77	3071.5
78	2166.0
79	1689.5
80	1213.0
81	772.5
82	332.0
83	226.0
84	120.0
85	75.5
86	31.0
87	18.5
88	6.0
89	5.0
90	4.0
91	4.0
92	4.0
93	5.5
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2292896.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.269327988914032
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.93759902655336	13.330696731871194
2	7.091355189214182	2.3074316691842607
3	2.3059996727857475	1.1255119505663929
4	1.137691344296989	0.7403789452206492
5	0.669695305966771	0.5447746292704766
6	0.46248448156993366	0.45145870322664705
7	0.31087824103101663	0.35404460475651967
8	0.25873620914355067	0.3367571399331747
9	0.19004988812463788	0.2782785567740536
>10	2.353535306122628	9.515081656112063
>50	1.2946534966902667	15.60270925102316
>100	1.977546789152006	53.68100152167826
>500	0.008417403606013996	0.8257813654902958
>1k	8.145874457432901E-4	0.289794348559158
>5k	5.430582971621933E-4	0.6162989263336605
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7483	0.32635583995087436	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6468	0.2820886773756856	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3435	0.1498105452667718	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016790992700933666	0.0
2	0.0	0.0	0.0	0.06650977628291907	0.0
3	0.0	0.0	0.0	0.11042803511367284	0.0
4	0.0	0.0	0.0	0.1932054484808731	0.0
5	0.0	0.0	0.0	0.3301065552035504	0.0
6	0.0	0.0	0.0	0.47830342065231046	0.0
7	0.0	0.0	0.0	0.5701959443428747	0.0
8	0.0	0.0	0.0	0.7732579236040361	0.0
9	0.0	0.0	0.0	0.8634931545085341	0.0
10	0.0	0.0	0.0	1.0113847291809135	0.0
11	0.0	0.0	0.0	1.1744536167362147	0.0
12	4.361296805437316E-5	0.0	0.0	1.3134045329574477	0.0
13	8.722593610874632E-5	0.0	0.0	1.3718022971822534	0.0
14	8.722593610874632E-5	0.0	0.0	1.3921259402955912	0.0
15	1.3083890416311947E-4	0.0	0.0	1.4230475346461418	0.0
16	1.3083890416311947E-4	0.0	0.0	1.4968842895621957	0.0
17	1.3083890416311947E-4	0.0	0.0	1.5783969268558191	0.0
18	1.3083890416311947E-4	0.0	0.0	1.6968497480914966	0.0
19	1.3083890416311947E-4	0.0	0.0	1.750188408021995	0.0
20	1.3083890416311947E-4	0.0	0.0	1.8053151996427226	0.0
21	1.3083890416311947E-4	0.0	0.0	1.8801986657920813	0.0
22	1.3083890416311947E-4	0.0	0.0	1.953555678059537	0.0
23	1.3083890416311947E-4	0.0	0.0	2.0365075432989546	0.0
24	1.3083890416311947E-4	0.0	0.0	2.0991357654250344	0.0
25	1.3083890416311947E-4	0.0	0.0	2.1477206118376064	0.0
26	1.3083890416311947E-4	0.0	0.0	2.2044610832763456	0.0
27	1.7445187221749265E-4	0.0	0.0	2.256753031973539	0.0
28	1.7445187221749265E-4	0.0	0.0	2.3118362106262125	0.0
29	1.7445187221749265E-4	0.0	0.0	2.3725018491898453	0.0
30	1.7445187221749265E-4	0.0	0.0	2.450743513879391	0.0
31	1.7445187221749265E-4	0.0	0.0	2.529159630441154	0.0
32	1.7445187221749265E-4	0.0	0.0	2.5980681199670634	0.0
33	1.7445187221749265E-4	0.0	0.0	2.6691136449276374	0.0
34	1.7445187221749265E-4	0.0	0.0	2.7458724687033342	0.0
35	1.7445187221749265E-4	0.0	0.0	2.8366310552244847	0.0
36	1.7445187221749265E-4	0.0	0.0	2.9162247219237156	0.0
37	1.7445187221749265E-4	0.0	0.0	2.999351039035351	0.0
38	1.7445187221749265E-4	0.0	0.0	3.075673733130504	0.0
39	1.7445187221749265E-4	0.0	0.0	3.1561396591908224	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAA	25	3.892024E-5	45.000004	26
AATGCGC	25	3.892024E-5	45.000004	22
CTACGAT	25	3.892024E-5	45.000004	43
CGGTACC	25	3.892024E-5	45.000004	14
GTGCGTA	25	3.892024E-5	45.000004	29
TCTACGT	20	7.0347585E-4	45.0	12
TTACGTC	20	7.0347585E-4	45.0	13
GCGTAAT	35	1.2126293E-7	45.0	31
CGTCGAT	30	2.166451E-6	44.999996	31
GTACGTC	30	2.166451E-6	44.999996	29
TACGTCA	30	2.166451E-6	44.999996	30
GCGATAT	215	0.0	43.95349	9
CTATACG	110	0.0	42.954544	1
TATAGCG	95	0.0	42.63158	1
TAGTACG	160	0.0	42.1875	1
ACGCTAG	70	0.0	41.785713	1
CGTTTTT	6270	0.0	41.590908	1
GCTAACG	65	0.0	41.53846	1
CTAATGC	60	3.6379788E-12	41.249996	20
CGACGGT	255	0.0	40.588234	28
>>END_MODULE