FastQCFastQC Report
Thu 26 May 2016
SRR1546266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546266_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2228951
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC58160.2609299172570415No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52870.23719678000996883No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG34310.1539289109540766No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG24930.111846334890269No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG23370.1048475269308298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA207.0347136E-445.00000432
CGAACCG207.0347136E-445.00000421
CGCGATC207.0347136E-445.00000444
GTTCGGA406.8193913E-945.00000436
CGATCAT406.8193913E-945.00000435
ACGACAC207.0347136E-445.00000423
TACCGGT207.0347136E-445.00000440
TGTCCGA406.8193913E-945.00000427
TCGTTAC253.8919894E-545.039
CAACGAC551.8189894E-1245.012
ACGTTAC351.2126111E-745.024
CTATACG351.2126111E-745.01
TATTCGA551.8189894E-1245.037
TATTCCG253.8919894E-545.024
GCGATAC900.045.09
GTATACG502.1827873E-1145.01
CGTCGGT253.8919894E-545.041
ATACCTA253.8919894E-545.045
AATCGTC253.8919894E-545.038
AACCGTA302.1664237E-644.99999639