##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546266_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2228951
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2617975899874	31.0	31.0	33.0	30.0	34.0
2	31.488861352268398	31.0	31.0	34.0	30.0	34.0
3	31.58709455703602	31.0	31.0	34.0	30.0	34.0
4	35.3388804868299	37.0	35.0	37.0	33.0	37.0
5	35.05040218470482	37.0	35.0	37.0	32.0	37.0
6	35.18825985856127	37.0	35.0	37.0	32.0	37.0
7	35.72648434173744	37.0	35.0	37.0	35.0	37.0
8	35.795007158075705	37.0	35.0	37.0	35.0	37.0
9	37.54272121729011	39.0	37.0	39.0	35.0	39.0
10	36.87226367919259	39.0	37.0	39.0	32.0	39.0
11	36.50278000727697	39.0	35.0	39.0	32.0	39.0
12	35.34906779018471	37.0	35.0	39.0	30.0	39.0
13	34.70619766876885	37.0	33.0	39.0	27.0	39.0
14	35.61105336097563	37.0	34.0	40.0	27.0	41.0
15	35.98543171204751	37.0	34.0	40.0	30.0	41.0
16	36.19847318312516	37.0	35.0	40.0	31.0	41.0
17	36.157165410993784	37.0	34.0	40.0	31.0	41.0
18	36.13430936794932	37.0	35.0	40.0	31.0	41.0
19	36.066678002342805	37.0	35.0	40.0	31.0	41.0
20	35.99830637820212	36.0	34.0	40.0	31.0	41.0
21	35.79657560888508	36.0	34.0	40.0	30.0	41.0
22	35.676315450631265	36.0	34.0	40.0	30.0	41.0
23	35.70217425147525	36.0	34.0	40.0	30.0	41.0
24	35.56662663288695	35.0	34.0	40.0	30.0	41.0
25	35.43049712622664	35.0	34.0	40.0	30.0	41.0
26	35.35936321614966	35.0	34.0	40.0	29.0	41.0
27	35.29889979636161	35.0	34.0	40.0	29.0	41.0
28	35.27535149942731	36.0	34.0	40.0	29.0	41.0
29	35.354495006844026	36.0	34.0	40.0	29.0	41.0
30	35.280222400582154	36.0	34.0	40.0	29.0	41.0
31	35.148518742673126	35.0	34.0	40.0	29.0	41.0
32	34.95584066226669	35.0	34.0	40.0	29.0	41.0
33	34.799450952488414	35.0	34.0	40.0	27.0	41.0
34	34.70092613072248	35.0	34.0	40.0	27.0	41.0
35	34.5307761363978	35.0	33.0	40.0	26.0	41.0
36	34.36306854659434	35.0	33.0	40.0	25.0	41.0
37	34.28754288452281	35.0	33.0	40.0	24.0	41.0
38	34.285932261409066	35.0	33.0	40.0	25.0	41.0
39	34.226856041249896	35.0	33.0	40.0	24.0	41.0
40	34.03822964255383	35.0	33.0	40.0	23.0	41.0
41	34.01485766174312	35.0	33.0	40.0	24.0	41.0
42	33.9797106351822	35.0	33.0	39.0	24.0	41.0
43	33.92343348956527	35.0	33.0	39.0	24.0	41.0
44	33.7176878271438	35.0	33.0	39.0	23.0	41.0
45	33.701362210295336	35.0	33.0	39.0	23.0	41.0
46	33.68331695043992	35.0	33.0	39.0	23.0	41.0
47	33.636942220802524	35.0	33.0	39.0	23.0	41.0
48	33.564503661139256	35.0	33.0	39.0	23.0	41.0
49	33.51847124499372	35.0	33.0	39.0	24.0	41.0
50	33.40171452849345	35.0	32.0	39.0	24.0	40.0
51	33.31463993600577	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	7.0
14	30.0
15	88.0
16	264.0
17	665.0
18	1499.0
19	2794.0
20	4725.0
21	7694.0
22	11384.0
23	15998.0
24	22109.0
25	29907.0
26	38274.0
27	45537.0
28	51254.0
29	60199.0
30	71599.0
31	87761.0
32	109089.0
33	142486.0
34	250468.0
35	356489.0
36	148348.0
37	175359.0
38	240364.0
39	354335.0
40	221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.173990814513196	24.23413525016925	28.59309154844588	13.998782386871673
2	28.342525250667244	27.0357221850099	30.788653496644834	13.833099067678024
3	30.20555409248566	25.68907975096806	30.476443851838823	13.62892230470746
4	27.608502833844263	27.947630970801963	29.643989482047832	14.799876713305945
5	24.891619420974262	32.85953796202788	28.69704179230499	13.551800824692872
6	22.709561583004742	40.32511257537739	25.928115961275054	11.037209880342816
7	86.70989178317512	5.565487980668934	5.873525259191431	1.8510949769645002
8	88.81280925421868	4.089233006916706	5.069649355234817	2.0283083836297884
9	84.82304007580247	5.574101898157474	6.948066601733281	2.6547914243067705
10	57.43154515285441	21.3587019185258	11.060404647746855	10.14934828087293
11	52.93211918969954	19.605096747304003	16.00578029754804	11.45700376544841
12	46.53175417494597	21.98321093644499	19.4016826749444	12.083352213664634
13	23.383017392486423	41.60746467733028	20.695475136061763	14.31404279412154
14	15.563599199802958	44.784205664458305	24.606328268319942	15.0458668674188
15	14.90373722885788	23.429676112216015	48.4313473019371	13.235239356989007
16	16.918945279640514	17.741305214874618	46.60048605823995	18.739263447244912
17	16.833075289676625	18.88543085962859	27.05402675967305	37.227467091021744
18	22.299144305998652	24.842807221872533	31.414643031632366	21.44340544049645
19	31.500378429135502	24.47783733244921	23.95211918072672	20.06966505768857
20	34.99547545011084	23.088932865729213	22.1535601276116	19.762031556548347
21	24.987090339805583	27.969614406059172	25.6921753775655	21.35111987656974
22	23.198580857093763	25.862210519657005	23.790024993819962	27.14918362942927
23	21.950146055252	30.46774020604311	22.977938949757082	24.604174788947805
24	22.591793179841098	23.602447967676273	35.756416359085506	18.04934249339712
25	19.446008458687515	24.323639236573616	33.91725524697492	22.313097057763944
26	17.18283623103424	36.289267911228194	26.473350019807523	20.05454583793004
27	20.19689979725889	33.96261290625052	27.26026727370857	18.58022002278202
28	16.028167510187526	29.546140763076444	37.32361097215686	17.10208075457917
29	17.290779384562512	25.221236357371694	36.75549619529546	20.732488062770337
30	20.24535308313193	29.509845662825246	30.234940113084583	20.009861140958236
31	30.178052366337347	27.003330266120702	22.757790548109853	20.0608268194321
32	31.966741305663515	27.5520188644793	23.827755746985915	16.65348408287127
33	29.400960362071665	28.263115698819757	23.010061683724768	19.32586225538381
34	20.979061450879808	28.72467811091406	26.786187762763742	23.510072675442395
35	21.097682272961585	25.950951815450406	29.866964325370994	23.084401586217016
36	30.23790114722127	25.70460274810886	25.17031554305142	18.887180561618447
37	22.076393783443425	32.54136138479491	26.935585394205614	18.44665943755605
38	22.496187668549016	33.436446112992165	21.40226501165795	22.665101206800866
39	22.00398303955538	32.14552495770432	25.013201277192724	20.83729072554758
40	24.99422374022578	26.368771677798208	24.40874653592654	24.228258046049465
41	17.919640225379563	25.060532959226112	26.638764154079652	30.381062661314672
42	24.34328973584435	26.396183675639346	21.97464188311004	27.285884705406264
43	23.76759291702689	27.153445724019953	23.339992669197304	25.738968689755854
44	21.17125948484287	31.283819159775163	26.415789310756495	21.129132044625475
45	18.57981624539974	36.927236175223236	21.293738624133056	23.199208955243968
46	22.341182017908874	33.643449317638655	23.732598877229695	20.28276978722278
47	23.36229015352962	27.43447478208359	25.61025343311719	23.5929816312696
48	24.833610070387373	22.930966180952385	29.596164294325	22.639259454335246
49	21.74583469982068	23.82721737714288	30.114210675784257	24.312737247252183
50	19.382211632288012	32.485415785272984	26.476041869022694	21.65633071341631
51	18.924956178937986	34.80780869565997	23.062956520802835	23.204278604599203
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1140.0
1	2181.5
2	3223.0
3	7051.5
4	10880.0
5	8625.0
6	6370.0
7	6772.0
8	7174.0
9	7487.0
10	7800.0
11	7938.0
12	8076.0
13	7981.5
14	7887.0
15	7639.0
16	7391.0
17	7346.5
18	7302.0
19	7122.5
20	6943.0
21	6908.5
22	6874.0
23	8363.0
24	9852.0
25	10017.0
26	12102.5
27	14023.0
28	19640.0
29	25257.0
30	27873.5
31	30490.0
32	38420.0
33	46350.0
34	45869.5
35	45389.0
36	48114.5
37	50840.0
38	63580.0
39	76320.0
40	117310.0
41	158300.0
42	185804.5
43	213309.0
44	220579.5
45	227850.0
46	210697.0
47	193544.0
48	183409.5
49	173275.0
50	161873.0
51	150471.0
52	140029.0
53	129587.0
54	118949.0
55	108311.0
56	104788.5
57	101266.0
58	100477.0
59	99688.0
60	92582.5
61	85477.0
62	74618.5
63	63760.0
64	52535.5
65	41311.0
66	35913.0
67	30515.0
68	25543.0
69	20571.0
70	17931.0
71	15291.0
72	13063.5
73	10836.0
74	8935.5
75	5309.5
76	3584.0
77	3038.5
78	2493.0
79	2082.5
80	1672.0
81	1091.0
82	510.0
83	451.0
84	392.0
85	224.0
86	56.0
87	57.0
88	58.0
89	39.0
90	20.0
91	11.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2228951.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.226241443396408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.58669937861501	12.422587833085917
2	8.075944267813728	2.459325546102901
3	2.5628119489508774	1.1706598052624213
4	1.1691947844977248	0.7120976833248877
5	0.6542505847074733	0.4980888683619631
6	0.4043066127872109	0.3693642062075913
7	0.2914740406879438	0.31066378825978863
8	0.2055805214825985	0.25041749249227097
9	0.16296233761747056	0.22331735088694987
>10	1.8815987426441545	7.00518965040947
>50	1.0082039350630014	11.360310346612046
>100	1.9721564435539714	59.9169788020009
>500	0.02152748088778849	2.0427686134382994
>1k	0.0026909351109735613	0.7527618957278037
>5k	5.97985580216347E-4	0.5054681178267798
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5816	0.2609299172570415	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5287	0.23719678000996883	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3431	0.1539289109540766	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2493	0.111846334890269	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2337	0.1048475269308298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02812982429851531	0.0
2	0.0	0.0	0.0	0.09093963931912366	0.0
3	0.0	0.0	0.0	0.13221466061838058	0.0
4	0.0	0.0	0.0	0.23885675369265633	0.0
5	0.0	0.0	0.0	0.3916640608070792	0.0
6	0.0	0.0	0.0	0.5628656708918232	0.0
7	0.0	0.0	0.0	0.6656045826041039	0.0
8	0.0	0.0	0.0	0.9211059372772215	0.0
9	0.0	0.0	0.0	1.0014127721964279	0.0
10	0.0	0.0	0.0	1.1499131205665805	0.0
11	0.0	0.0	0.0	1.29693295186839	0.0
12	0.0	0.0	0.0	1.434037805227661	0.0
13	0.0	0.0	0.0	1.485945630926835	0.0
14	0.0	0.0	0.0	1.503756699900536	0.0
15	0.0	0.0	0.0	1.5358345697146325	0.0
16	0.0	0.0	0.0	1.6075723512988846	0.0
17	0.0	0.0	0.0	1.6905261712796738	0.0
18	0.0	0.0	0.0	1.8213051789832975	0.0
19	0.0	0.0	0.0	1.8737065103719193	0.0
20	0.0	0.0	0.0	1.934766623402668	0.0
21	0.0	0.0	0.0	2.0189766396838693	0.0
22	0.0	0.0	0.0	2.1023342370469336	0.0
23	0.0	0.0	0.0	2.1993305371001877	0.0
24	4.486415358614882E-5	0.0	0.0	2.268780246851546	0.0
25	4.486415358614882E-5	0.0	0.0	2.3271036465135393	0.0
26	4.486415358614882E-5	0.0	0.0	2.391483706909663	0.0
27	8.972830717229764E-5	0.0	0.0	2.4467563441277984	0.0
28	8.972830717229764E-5	0.0	0.0	2.508758604383856	0.0
29	8.972830717229764E-5	0.0	0.0	2.5731386647799797	0.0
30	8.972830717229764E-5	0.0	0.0	2.663674526716828	0.0
31	8.972830717229764E-5	0.0	0.0	2.7581584341692573	0.0
32	8.972830717229764E-5	0.0	0.0	2.834876136801572	0.0
33	8.972830717229764E-5	0.0	0.0	2.9138819112667798	0.0
34	8.972830717229764E-5	0.0	0.0	2.9938298329572968	0.0
35	8.972830717229764E-5	0.0	0.0	3.098767088195299	0.0
36	1.3459246075844645E-4	0.0	0.0	3.19603257317007	0.0
37	1.3459246075844645E-4	0.0	0.0	3.2893051484756732	0.0
38	1.3459246075844645E-4	0.0	0.0	3.3804691085627274	0.0
39	1.3459246075844645E-4	0.0	0.0	3.4721265743392293	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	20	7.0347136E-4	45.000004	32
CGAACCG	20	7.0347136E-4	45.000004	21
CGCGATC	20	7.0347136E-4	45.000004	44
GTTCGGA	40	6.8193913E-9	45.000004	36
CGATCAT	40	6.8193913E-9	45.000004	35
ACGACAC	20	7.0347136E-4	45.000004	23
TACCGGT	20	7.0347136E-4	45.000004	40
TGTCCGA	40	6.8193913E-9	45.000004	27
TCGTTAC	25	3.8919894E-5	45.0	39
CAACGAC	55	1.8189894E-12	45.0	12
ACGTTAC	35	1.2126111E-7	45.0	24
CTATACG	35	1.2126111E-7	45.0	1
TATTCGA	55	1.8189894E-12	45.0	37
TATTCCG	25	3.8919894E-5	45.0	24
GCGATAC	90	0.0	45.0	9
GTATACG	50	2.1827873E-11	45.0	1
CGTCGGT	25	3.8919894E-5	45.0	41
ATACCTA	25	3.8919894E-5	45.0	45
AATCGTC	25	3.8919894E-5	45.0	38
AACCGTA	30	2.1664237E-6	44.999996	39
>>END_MODULE