FastQCFastQC Report
Thu 26 May 2016
SRR1546265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546265_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1124883
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160581.4275262405067906No Hit
TGATATTGTACCTTTTTCCCATACTTGATTTTTATAAAGAAACACACATC14440.12836890592177141No Hit
GTTTTGGGCAAATTTAAGACTATGGGACAACCGTTTTTGTTTTAAATAAA13890.12347950853555437No Hit
GGGCTAGGGCAATTTCTAATCATTTATATTGTAACCAGAAACCCAAAATT13100.11645655592626078No Hit
TTGTAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT12800.1137896118974151No Hit
TGATTAGGGAGGATTGCTTTTTTAATAGTTTAGGTAAAAATTAGTGTATT12260.10898911264549292No Hit
TTTTACGGGGTGTTTTTGCCAATTTAAGACCTAATTTAATAGTTAAACCA12080.10738894622818551No Hit
TAACCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT11800.10489979846792954No Hit
TTATGTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT11740.10436640966216043No Hit
ATGCAAGGGAACCAAACACAAATACGAAAAATTATAGCCTATTCATCAAT11680.10383302085639129No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATT207.856409E-444.00000437
AACGTAG207.856409E-444.0000041
AGCCGTC207.856409E-444.00000424
TAATACG207.856409E-444.0000041
ACACGAT207.856409E-444.00000431
CTATAAG207.856409E-444.00000438
CCAATAC207.856409E-444.00000434
TACGGGT207.856409E-444.0000044
CCGTCCA207.856409E-444.00000419
ATATCCG207.856409E-444.0000041
TCGGACG207.856409E-444.0000041
TACGCCC207.856409E-444.00000420
GTATGCG207.856409E-444.0000041
CTACGAT207.856409E-444.00000411
GTCAAGC408.312782E-944.00000416
TATAGCG207.856409E-444.0000041
CGTCCAA207.856409E-444.00000420
CCGTTGA207.856409E-444.00000420
CCGTTAT207.856409E-444.00000420
TGCGTTA207.856409E-444.00000412
Produced by FastQC (version 0.11.5)