##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546265_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1124883
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.916866909714166	31.0	31.0	34.0	30.0	34.0
2	32.27061036570026	33.0	31.0	34.0	30.0	34.0
3	32.18217716864776	33.0	31.0	34.0	30.0	34.0
4	35.93052166314185	37.0	35.0	37.0	35.0	37.0
5	35.92558159381909	37.0	35.0	37.0	35.0	37.0
6	35.988806835910935	37.0	35.0	37.0	35.0	37.0
7	36.208593249253475	37.0	35.0	37.0	35.0	37.0
8	36.10941137878339	37.0	35.0	37.0	35.0	37.0
9	37.905204363476024	39.0	38.0	39.0	35.0	39.0
10	37.53052895278887	39.0	37.0	39.0	35.0	39.0
11	37.30564867635123	39.0	37.0	39.0	34.0	39.0
12	36.606934232271264	39.0	35.0	39.0	33.0	39.0
13	36.352012609311366	39.0	35.0	39.0	33.0	39.0
14	37.46634983371604	40.0	35.0	41.0	33.0	41.0
15	37.69094741408662	40.0	35.0	41.0	33.0	41.0
16	37.831721165667894	40.0	35.0	41.0	34.0	41.0
17	37.74302571911923	40.0	35.0	41.0	34.0	41.0
18	37.57342674749285	39.0	36.0	41.0	34.0	41.0
19	37.31521233763867	38.0	35.0	41.0	34.0	41.0
20	37.02342999227475	38.0	35.0	41.0	33.0	41.0
21	36.911255659477476	38.0	35.0	41.0	33.0	41.0
22	36.82253887737658	38.0	35.0	41.0	33.0	41.0
23	36.76719801081535	37.0	35.0	41.0	33.0	41.0
24	36.699953684072035	37.0	35.0	41.0	33.0	41.0
25	36.677477568778265	37.0	35.0	41.0	33.0	41.0
26	36.6197266737963	37.0	35.0	41.0	33.0	41.0
27	36.53962234294589	37.0	35.0	41.0	33.0	41.0
28	36.46752328908873	37.0	35.0	41.0	32.0	41.0
29	36.39756934721211	37.0	35.0	41.0	32.0	41.0
30	36.15483476948269	37.0	35.0	41.0	31.0	41.0
31	35.80131178086965	37.0	35.0	41.0	30.0	41.0
32	35.404882996720545	37.0	35.0	41.0	29.0	41.0
33	34.966198262397064	37.0	35.0	41.0	24.0	41.0
34	34.48391521607136	37.0	34.0	40.0	21.0	41.0
35	34.24883298974204	37.0	34.0	40.0	18.0	41.0
36	34.04878551813833	36.0	34.0	40.0	18.0	41.0
37	34.011274061391276	37.0	34.0	40.0	18.0	41.0
38	33.980106375507496	36.0	34.0	40.0	18.0	41.0
39	33.91123610188793	36.0	34.0	40.0	18.0	41.0
40	33.77082861061995	36.0	34.0	40.0	18.0	41.0
41	33.687697298296804	36.0	34.0	40.0	16.0	41.0
42	33.59596331351794	36.0	34.0	40.0	15.0	41.0
43	33.46479500534722	36.0	33.0	40.0	15.0	41.0
44	33.319133634342414	35.0	33.0	40.0	15.0	41.0
45	33.29500045782539	35.0	33.0	40.0	15.0	41.0
46	33.20182276734558	35.0	33.0	40.0	15.0	41.0
47	33.19505317441903	35.0	33.0	40.0	15.0	41.0
48	33.147808260948025	35.0	33.0	40.0	14.0	41.0
49	33.066456689273465	36.0	33.0	40.0	13.0	41.0
50	32.87888073692997	35.0	33.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	3.0
13	3.0
14	9.0
15	14.0
16	50.0
17	190.0
18	446.0
19	855.0
20	1617.0
21	2692.0
22	4148.0
23	6849.0
24	11016.0
25	18344.0
26	28182.0
27	34180.0
28	32112.0
29	27740.0
30	26065.0
31	27760.0
32	33103.0
33	43133.0
34	83014.0
35	179575.0
36	56769.0
37	71681.0
38	122943.0
39	312387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.074386402852564	22.704139008234634	28.00549034877405	21.215984240138752
2	35.59854669330055	22.856510410416018	30.091662866271424	11.45328003001201
3	25.739388007463887	23.11724863830283	39.77506994060715	11.368293413626128
4	22.39966289827475	25.418287946390873	40.165243852027274	12.0168053033071
5	20.18369910470689	29.52982665752794	38.804124517838744	11.482349719926429
6	17.247838219619286	40.007894154325385	34.591153035471244	8.153114590584087
7	79.29349096750506	3.3668390401490647	16.030378270451237	1.3092917218946325
8	79.91133300085431	2.600803816930294	15.544194373992672	1.9436688082227218
9	76.91537697698338	3.7340772329211127	16.99803446225074	2.3525113278447627
10	43.77193005850386	25.22404552295661	22.695338092939444	8.308686325600084
11	36.94339766891312	18.87156264251482	31.13479357408726	13.050246114484795
12	36.37151597099432	19.05095907752184	33.0815738170103	11.495951134473541
13	17.649479990363442	37.51972427354667	31.040028162928945	13.790767573160942
14	11.045504288001508	38.50044849108752	33.86147714917907	16.592570071731906
15	9.876049331352684	24.268835069958385	53.333102198184164	12.522013400504765
16	11.31851045842101	19.850242202966886	49.70374696746239	19.12750037114971
17	11.459414001278354	22.42393208893725	33.84467540179734	32.27197850798705
18	14.068929835369545	25.483539176963294	43.67316423130227	16.774366756364884
19	17.957867618232296	26.003148771916724	34.957235552497465	21.08174805735352
20	21.838715670874215	20.970180898813474	39.36773868926813	17.82336474104418
21	15.793998131361217	28.97456891072227	37.51305691347456	17.718376044441957
22	20.313223686374492	22.786369782457374	32.014707307337744	24.88569922383039
23	13.951317603697452	27.476546449719656	32.37296678854601	26.199169158036877
24	12.964548313024554	22.70369451756316	50.04840503412355	14.283352135288737
25	11.988446798467042	28.465627091884222	43.09790440428027	16.448021705368472
26	14.253660158434256	28.891182460753694	36.12331238004308	20.73184500076897
27	12.429648238972408	36.272483449389846	32.85123875105233	18.44662956058541
28	10.80512373286822	29.80078817085866	41.52325175151549	17.87083634475763
29	11.176184545414944	23.03110634617111	39.84609954990875	25.946609558505195
30	13.23782117784694	32.28566882066846	36.9369081051096	17.539601896375
31	19.28031626400257	33.62989750934097	30.508772912382888	16.581013314273573
32	20.129915733458503	24.819914604452194	35.006040628225335	20.044129033863967
33	20.75824774665454	30.829872973455906	30.262258385983255	18.1496208939063
34	15.42809341060359	27.885922358147468	32.4820447993258	24.203939431923143
35	17.263839883792357	26.41919204041665	34.39095443703923	21.926013638751762
36	24.741506450004135	28.758546444385775	30.54459886050371	15.955348245106379
37	15.676652594092008	31.68693988619261	36.727641896979506	15.908765622735876
38	15.169310941671268	29.739537356329503	35.30322709117304	19.787924610826195
39	16.292094377815292	30.79458041414085	34.74699146489012	18.166333743153732
40	18.105171826758873	26.85079248241817	33.38276069600127	21.66127499482168
41	13.692712931033716	28.059007025619554	34.28507675909406	23.963203284252675
42	16.48847035647263	26.961470659615266	31.22244713450199	25.327611849410115
43	17.206144994634997	26.819144746609204	32.76322959810042	23.211480660655376
44	14.480883789691907	28.145060419616975	32.42434991016844	24.949705880522686
45	14.470660504247999	37.738947072717785	27.602248411612585	20.18814401142163
46	19.548699731438738	32.112406356927785	31.829176901064375	16.5097170105691
47	15.808310730982688	30.17967201922333	33.3027523751359	20.709264874658075
48	15.911432566764722	28.485806968369154	35.01395256217757	20.588807902688547
49	18.28492385430307	26.87888429285535	34.61746688322252	20.21872496961906
50	16.865220649614226	29.703444713805794	33.600294430620785	19.831040205959198
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2101.0
1	3542.5
2	4984.0
3	16676.5
4	28369.0
5	21024.0
6	13679.0
7	14460.5
8	15242.0
9	16433.0
10	17624.0
11	17330.5
12	17037.0
13	15966.5
14	14896.0
15	13443.0
16	11990.0
17	10826.0
18	9662.0
19	9026.0
20	8390.0
21	7737.5
22	7085.0
23	8985.5
24	10886.0
25	11632.5
26	12379.0
27	13375.0
28	14371.0
29	21835.5
30	29300.0
31	34993.5
32	40687.0
33	52707.0
34	64727.0
35	64258.5
36	63790.0
37	62788.5
38	61787.0
39	67258.0
40	72729.0
41	81720.5
42	90712.0
43	93457.5
44	96203.0
45	91820.0
46	87437.0
47	82124.0
48	76811.0
49	71085.5
50	65360.0
51	55653.5
52	45947.0
53	37961.0
54	29975.0
55	26631.5
56	23288.0
57	21384.5
58	19481.0
59	18217.5
60	16954.0
61	15929.5
62	14905.0
63	12914.5
64	10924.0
65	9560.5
66	8197.0
67	7438.0
68	6679.0
69	5321.0
70	3963.0
71	3455.5
72	2948.0
73	2208.5
74	1469.0
75	1284.5
76	1100.0
77	737.0
78	374.0
79	312.5
80	251.0
81	181.0
82	111.0
83	78.0
84	45.0
85	26.0
86	7.0
87	8.0
88	9.0
89	9.0
90	9.0
91	7.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1124883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.951681113218136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.39436967280605	21.937150958328616
2	8.297958680391028	4.472878724891187
3	2.915674964529816	2.357470256214036
4	1.4321045909383927	1.5439050502298903
5	0.8111998609810279	1.0931599986123772
6	0.532615874698483	0.8612935926426755
7	0.3914050515861018	0.7384316895515916
8	0.29133967265292393	0.6281675162376771
9	0.23139820674316952	0.5612913610481184
>10	2.610283206182345	17.06462843082417
>50	0.6254107968225918	11.504534979129165
>100	0.39958641757471824	22.66471262088269
>500	0.059689257397053164	11.088321539206403
>1k	0.006632139710783684	2.048891093411679
>5k	0.0	0.0
>10k+	3.316069855391842E-4	1.4351621887897381
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16058	1.4275262405067906	No Hit
TGATATTGTACCTTTTTCCCATACTTGATTTTTATAAAGAAACACACATC	1444	0.12836890592177141	No Hit
GTTTTGGGCAAATTTAAGACTATGGGACAACCGTTTTTGTTTTAAATAAA	1389	0.12347950853555437	No Hit
GGGCTAGGGCAATTTCTAATCATTTATATTGTAACCAGAAACCCAAAATT	1310	0.11645655592626078	No Hit
TTGTAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1280	0.1137896118974151	No Hit
TGATTAGGGAGGATTGCTTTTTTAATAGTTTAGGTAAAAATTAGTGTATT	1226	0.10898911264549292	No Hit
TTTTACGGGGTGTTTTTGCCAATTTAAGACCTAATTTAATAGTTAAACCA	1208	0.10738894622818551	No Hit
TAACCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1180	0.10489979846792954	No Hit
TTATGTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1174	0.10436640966216043	No Hit
ATGCAAGGGAACCAAACACAAATACGAAAAATTATAGCCTATTCATCAAT	1168	0.10383302085639129	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10312183578203245	0.0
2	0.0	0.0	0.0	0.38270646813935316	0.0
3	0.0	0.0	0.0	0.5507239419566301	0.0
4	0.0	0.0	0.0	0.8624008007943937	0.0
5	0.0	0.0	0.0	1.4541067826609524	0.0
6	0.0	0.0	0.0	1.9703382485111784	0.0
7	0.0	0.0	0.0	2.2885046711524666	0.0
8	0.0	0.0	0.0	3.0425386462414314	0.0
9	0.0	0.0	0.0	3.3250569170304822	0.0
10	0.0	0.0	0.0	3.845911085864041	0.0
11	0.0	0.0	0.0	4.419570746468744	0.0
12	0.0	0.0	0.0	4.917666992922819	0.0
13	0.0	0.0	0.0	5.095374363378236	0.0
14	0.0	0.0	0.0	5.152624761864122	0.0
15	0.0	0.0	0.0	5.247123478619554	0.0
16	0.0	0.0	0.0	5.486970644947075	0.0
17	0.0	0.0	0.0	5.770555693347664	0.0
18	8.889813429485555E-5	0.0	0.0	6.2562062010004595	0.0
19	8.889813429485555E-5	0.0	0.0	6.455693614358116	0.0
20	8.889813429485555E-5	0.0	0.0	6.652514083686926	0.0
21	8.889813429485555E-5	0.0	0.0	6.970680506328214	0.0
22	8.889813429485555E-5	0.0	0.0	7.2530209808486745	0.0
23	8.889813429485555E-5	0.0	0.0	7.601590565418803	0.0
24	8.889813429485555E-5	0.0	0.0	7.872463180615228	0.0
25	8.889813429485555E-5	0.0	0.0	8.086618786131535	0.0
26	8.889813429485555E-5	0.0	0.0	8.320420879327006	0.0
27	8.889813429485555E-5	0.0	0.0	8.51821922813306	0.0
28	8.889813429485555E-5	0.0	0.0	8.731930342977892	0.0
29	8.889813429485555E-5	0.0	0.0	8.962887695875926	0.0
30	8.889813429485555E-5	0.0	0.0	9.34230493304637	0.0
31	8.889813429485555E-5	0.0	0.0	9.697808572091498	0.0
32	8.889813429485555E-5	0.0	0.0	9.954190791397862	0.0
33	8.889813429485555E-5	0.0	0.0	10.217773759582107	0.0
34	8.889813429485555E-5	0.0	0.0	10.468555396427895	0.0
35	8.889813429485555E-5	0.0	0.0	10.875975545901218	0.0
36	8.889813429485555E-5	0.0	0.0	11.146314772291873	0.0
37	8.889813429485555E-5	0.0	0.0	11.405897324432852	0.0
38	8.889813429485555E-5	0.0	0.0	11.66894690381133	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATT	20	7.856409E-4	44.000004	37
AACGTAG	20	7.856409E-4	44.000004	1
AGCCGTC	20	7.856409E-4	44.000004	24
TAATACG	20	7.856409E-4	44.000004	1
ACACGAT	20	7.856409E-4	44.000004	31
CTATAAG	20	7.856409E-4	44.000004	38
CCAATAC	20	7.856409E-4	44.000004	34
TACGGGT	20	7.856409E-4	44.000004	4
CCGTCCA	20	7.856409E-4	44.000004	19
ATATCCG	20	7.856409E-4	44.000004	1
TCGGACG	20	7.856409E-4	44.000004	1
TACGCCC	20	7.856409E-4	44.000004	20
GTATGCG	20	7.856409E-4	44.000004	1
CTACGAT	20	7.856409E-4	44.000004	11
GTCAAGC	40	8.312782E-9	44.000004	16
TATAGCG	20	7.856409E-4	44.000004	1
CGTCCAA	20	7.856409E-4	44.000004	20
CCGTTGA	20	7.856409E-4	44.000004	20
CCGTTAT	20	7.856409E-4	44.000004	20
TGCGTTA	20	7.856409E-4	44.000004	12
>>END_MODULE