FastQCFastQC Report
Thu 26 May 2016
SRR1546264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546264_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1149276
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163871.4258541899421897No Hit
TGATATTGTACCTTTTTCCCATACTTGATTTTTATAAAGAAACACACATC14730.12816764641391626No Hit
GTTTTGGGCAAATTTAAGACTATGGGACAACCGTTTTTGTTTTAAATAAA14320.12460018307177737No Hit
GGGCTAGGGCAATTTCTAATCATTTATATTGTAACCAGAAACCCAAAATT13810.12016260671936072No Hit
TTATGTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13390.11650813207619405No Hit
TTGTAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT12860.11189653312172186No Hit
TGATTAGGGAGGATTGCTTTTTTAATAGTTTAGGTAAAAATTAGTGTATT12810.11146147661658297No Hit
TAACCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT12510.10885113758574963No Hit
TTTTACGGGGTGTTTTTGCCAATTTAAGACCTAATTTAATAGTTAAACCA12480.1085901036826663No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12380.10771999067238852No Hit
ATGCAAGGGAACCAAACACAAATACGAAAAATTATAGCCTATTCATCAAT12220.10632780985594409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGA950.044.0000041
TCACGAC850.044.025
GTTTGCG502.7284841E-1144.01
AACCGCT207.8564824E-444.035
AAATCGT551.8189894E-1244.012
CGACGTC351.4460784E-744.018
CTATCGA207.8564824E-444.030
GCGAAGA302.5277168E-644.09
TAGACCG207.8564824E-444.013
GCTATGT207.8564824E-444.032
ATTAACG302.5277168E-644.01
TTCCGTA207.8564824E-444.040
ATTCGCA207.8564824E-444.044
TCTAGCG207.8564824E-444.01
CGTTCAA254.4428405E-544.09
ATCGTTA207.8564824E-444.015
TCGGCAC207.8564824E-444.042
ATCCGTT207.8564824E-444.034
ATACTAC254.4428405E-544.044
TACGCAT454.802132E-1044.011
Produced by FastQC (version 0.11.5)