##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546264_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1149276
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85267942600385	31.0	31.0	34.0	30.0	34.0
2	32.241776561939865	33.0	31.0	34.0	30.0	34.0
3	32.274645080903106	34.0	31.0	34.0	30.0	34.0
4	35.96296625005656	37.0	35.0	37.0	35.0	37.0
5	35.885279950159926	37.0	35.0	37.0	35.0	37.0
6	35.97164562733408	37.0	35.0	37.0	35.0	37.0
7	36.18348595115533	37.0	35.0	37.0	35.0	37.0
8	36.090912017652855	37.0	35.0	37.0	35.0	37.0
9	37.88552880248086	39.0	38.0	39.0	35.0	39.0
10	37.5244754088661	39.0	37.0	39.0	35.0	39.0
11	37.30364768776169	39.0	37.0	39.0	34.0	39.0
12	36.59572374259969	39.0	35.0	39.0	33.0	39.0
13	36.29545470365691	39.0	35.0	39.0	33.0	39.0
14	37.40995548501839	40.0	35.0	41.0	33.0	41.0
15	37.65953609054744	40.0	35.0	41.0	33.0	41.0
16	37.791267719851454	40.0	35.0	41.0	33.0	41.0
17	37.72017513634671	40.0	35.0	41.0	34.0	41.0
18	37.55948614606065	39.0	36.0	41.0	34.0	41.0
19	37.30065449900633	38.0	35.0	41.0	34.0	41.0
20	37.01594221057431	38.0	35.0	41.0	33.0	41.0
21	36.898183726102346	38.0	35.0	41.0	33.0	41.0
22	36.8175921188644	38.0	35.0	41.0	33.0	41.0
23	36.750635182497504	37.0	35.0	41.0	33.0	41.0
24	36.68519050254247	37.0	35.0	41.0	33.0	41.0
25	36.65608609246169	37.0	35.0	41.0	33.0	41.0
26	36.62036969361581	37.0	35.0	41.0	33.0	41.0
27	36.52613906494175	37.0	35.0	41.0	33.0	41.0
28	36.45041225954427	37.0	35.0	41.0	32.0	41.0
29	36.37322627462855	37.0	35.0	41.0	32.0	41.0
30	36.1429473860065	37.0	35.0	41.0	31.0	41.0
31	35.79831389500868	37.0	35.0	41.0	30.0	41.0
32	35.41279379365792	37.0	35.0	41.0	29.0	41.0
33	34.955038650419915	37.0	35.0	41.0	24.0	41.0
34	34.55175171151229	37.0	34.0	41.0	22.0	41.0
35	34.293373393336324	37.0	34.0	40.0	20.0	41.0
36	34.06319543782347	36.0	34.0	40.0	18.0	41.0
37	33.974799786996336	36.0	34.0	40.0	18.0	41.0
38	33.96304978090554	36.0	34.0	40.0	18.0	41.0
39	33.90045298083315	36.0	34.0	40.0	18.0	41.0
40	33.775763176121316	36.0	34.0	40.0	18.0	41.0
41	33.70599055405316	36.0	34.0	40.0	16.0	41.0
42	33.598629920054016	36.0	34.0	40.0	15.0	41.0
43	33.49239260195114	36.0	33.0	40.0	15.0	41.0
44	33.34608744983799	35.0	33.0	40.0	15.0	41.0
45	33.324120576780516	35.0	33.0	40.0	15.0	41.0
46	33.2393158823468	35.0	33.0	40.0	15.0	41.0
47	33.21889868056063	35.0	33.0	40.0	15.0	41.0
48	33.18332759058747	35.0	33.0	40.0	15.0	41.0
49	33.096715671431404	36.0	33.0	40.0	13.0	41.0
50	32.88695317747869	35.0	33.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	5.0
13	1.0
14	10.0
15	22.0
16	70.0
17	161.0
18	449.0
19	944.0
20	1597.0
21	2744.0
22	4352.0
23	6877.0
24	11177.0
25	18285.0
26	28707.0
27	34495.0
28	32719.0
29	28551.0
30	27143.0
31	28881.0
32	33937.0
33	44591.0
34	87021.0
35	180469.0
36	59032.0
37	73893.0
38	125870.0
39	317268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.247696810861793	22.725959647639034	28.045221513370155	20.98112202812901
2	35.67898398644016	22.87570609670784	30.00793543065373	11.437374486198268
3	25.82173472690633	23.11211580160031	39.68167785631998	11.384471615173378
4	22.373302844573452	25.463422189273942	40.080537660231315	12.082737305921293
5	20.324621761874432	29.443406109585514	38.72672882754012	11.505243300999934
6	17.226149332275277	40.074098823955254	34.50085097052405	8.198900873245417
7	79.30035953069586	3.374820321663378	15.979625433751336	1.34519471388944
8	79.96155840720593	2.600071697312047	15.487315492536172	1.9510544029458545
9	76.95914645394144	3.709726819319293	16.968160824727914	2.362965902011353
10	43.67845495772991	25.309847242959915	22.668445177659674	8.3432526216505
11	36.890442330649904	18.90398824999391	31.055551495028173	13.150017924328012
12	36.298243415854856	19.035723359749966	33.07325655456131	11.592776669833878
13	17.74282243777822	37.42077621041421	31.05502942722201	13.781371924585564
14	11.04303926994038	38.447857607746094	33.901604140345746	16.607498981967776
15	9.87830599438255	24.336973886168337	53.22394272568122	12.560777393767902
16	11.352103411191045	19.89983259025682	49.60514271593595	19.142921282616186
17	11.532303815619573	22.39810106536637	33.823293969420746	32.24630114959331
18	14.146036287192981	25.5037084216498	43.52340081929841	16.826854471858805
19	17.871338129396246	26.197797569948385	34.87717484746919	21.05368945318618
20	21.804683992356928	21.031588582725124	39.27942461166856	17.884302813249384
21	15.822744057998253	28.9591012080649	37.44766270243179	17.770492031505054
22	20.343590225498488	22.880230684361287	31.923054166275115	24.85312492386511
23	13.999422244961176	27.37332024683366	32.389434739784	26.237822768421164
24	12.940494711453123	22.745972246875425	49.96980707854336	14.34372596312809
25	12.090655334314821	28.41188713590121	42.95069243593358	16.54676509385039
26	14.28934389998573	28.8124871658331	36.18817412005471	20.70999481412646
27	12.48333733585318	36.18269240808996	32.81596413742217	18.518006118634688
28	10.846828786122742	29.785186500022625	41.434259481621474	17.933725232233165
29	11.25369362972863	23.03424068718045	39.777651321353616	25.9344143617373
30	13.248688739693511	32.20801617714108	36.978932823795155	17.56436225937025
31	19.20426424984077	33.6433545988953	30.51512430434465	16.637256846919275
32	20.066633254327073	24.869657071060388	35.0325770311048	20.03113264350774
33	20.79996449938918	30.783902213219456	30.17334391390754	18.242789373483827
34	15.441373525593505	27.908265725552432	32.356370445393445	24.293990303460614
35	17.129218742930334	26.48023625308455	34.27505664435697	22.11548835962815
36	24.64020827025014	28.751927300317764	30.54235884156634	16.065505587865754
37	15.672997608929448	31.624953448953953	36.70441216905252	15.997636773064086
38	15.071575496225451	29.78596960173187	35.32423891214991	19.818215989892767
39	16.364824463401305	30.71290099158079	34.73273608776308	18.189538457254827
40	18.040836143798355	26.898325554523023	33.34246951994125	21.71836878173737
41	13.697231996491704	28.10699953709988	34.20292427580494	23.992844190603478
42	16.544937856528804	26.973851363815132	31.19276831674898	25.288442462907085
43	17.28227162143819	26.76554630915463	32.76802091055586	23.18416115885131
44	14.52618866138334	28.20819368019518	32.31095054625695	24.95466711216453
45	14.497387920743146	37.527713099377344	27.715970750281045	20.25892822959846
46	19.600687737323323	31.996578715643587	31.81481210779656	16.587921439236528
47	15.821699922385921	30.029079176803485	33.2586776370515	20.8905432637591
48	15.975709925205086	28.418587006080347	34.97967416007991	20.62602890863465
49	18.356861189131244	26.79225877857016	34.4834487103185	20.3674313219801
50	16.833641353338972	29.652494265955266	33.62638739519489	19.887476985510876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2232.0
1	3658.5
2	5085.0
3	17005.0
4	28925.0
5	21215.5
6	13506.0
7	14435.0
8	15364.0
9	16577.5
10	17791.0
11	17730.5
12	17670.0
13	16290.5
14	14911.0
15	13485.5
16	12060.0
17	10965.0
18	9870.0
19	9174.5
20	8479.0
21	7845.5
22	7212.0
23	9092.5
24	10973.0
25	11903.5
26	12834.0
27	13580.0
28	14326.0
29	22124.5
30	29923.0
31	35354.5
32	40786.0
33	53656.5
34	66527.0
35	65672.5
36	64818.0
37	63976.0
38	63134.0
39	68643.5
40	74153.0
41	83391.5
42	92630.0
43	95658.0
44	98686.0
45	93954.5
46	89223.0
47	84262.0
48	79301.0
49	73528.0
50	67755.0
51	57435.5
52	47116.0
53	39258.0
54	31400.0
55	27581.0
56	23762.0
57	21679.5
58	19597.0
59	18502.5
60	17408.0
61	16335.5
62	15263.0
63	13221.0
64	11179.0
65	9664.0
66	8149.0
67	7395.0
68	6641.0
69	5396.5
70	4152.0
71	3573.5
72	2995.0
73	2243.5
74	1492.0
75	1288.5
76	1085.0
77	727.0
78	369.0
79	325.5
80	282.0
81	201.5
82	121.0
83	93.5
84	66.0
85	38.5
86	11.0
87	9.0
88	7.0
89	6.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1149276.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.84225394916739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0305877549229	21.75043614167934
2	8.471426008230951	4.5478433644903316
3	2.9695807581019507	2.3913072249460066
4	1.470290278635688	1.5786362015252484
5	0.8581108474703041	1.1516814642166573
6	0.5490159243705862	0.8842094918455291
7	0.41105461389315506	0.7723542633167912
8	0.2884241799842761	0.6193564067374641
9	0.24315857335606852	0.5874231758346754
>10	2.616266138424362	16.887744744902225
>50	0.6229291730728386	11.337997369323508
>100	0.40124733206741314	22.607901052978416
>500	0.059419865285816637	10.88781413390352
>1k	0.008162069407392395	2.559214714684357
>5k	0.0	0.0
>10k+	3.264827762956958E-4	1.4360802496158804
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16387	1.4258541899421897	No Hit
TGATATTGTACCTTTTTCCCATACTTGATTTTTATAAAGAAACACACATC	1473	0.12816764641391626	No Hit
GTTTTGGGCAAATTTAAGACTATGGGACAACCGTTTTTGTTTTAAATAAA	1432	0.12460018307177737	No Hit
GGGCTAGGGCAATTTCTAATCATTTATATTGTAACCAGAAACCCAAAATT	1381	0.12016260671936072	No Hit
TTATGTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1339	0.11650813207619405	No Hit
TTGTAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1286	0.11189653312172186	No Hit
TGATTAGGGAGGATTGCTTTTTTAATAGTTTAGGTAAAAATTAGTGTATT	1281	0.11146147661658297	No Hit
TAACCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1251	0.10885113758574963	No Hit
TTTTACGGGGTGTTTTTGCCAATTTAAGACCTAATTTAATAGTTAAACCA	1248	0.1085901036826663	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1238	0.10771999067238852	No Hit
ATGCAAGGGAACCAAACACAAATACGAAAAATTATAGCCTATTCATCAAT	1222	0.10632780985594409	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10276034651380521	0.0
2	0.0	0.0	0.0	0.3856340861551098	0.0
3	0.0	0.0	0.0	0.5618319707363593	0.0
4	0.0	0.0	0.0	0.8708091006859972	0.0
5	0.0	0.0	0.0	1.4473459812960507	0.0
6	0.0	0.0	0.0	1.9816823809076323	0.0
7	0.0	0.0	0.0	2.324419895656048	0.0
8	0.0	0.0	0.0	3.0944699097518784	0.0
9	0.0	0.0	0.0	3.3807370901332665	0.0
10	0.0	0.0	0.0	3.917770840076709	0.0
11	0.0	0.0	0.0	4.52102019010229	0.0
12	0.0	0.0	0.0	5.031428481931234	0.0
13	0.0	0.0	0.0	5.217110598324511	0.0
14	0.0	0.0	0.0	5.277583452538816	0.0
15	0.0	0.0	0.0	5.374775075786843	0.0
16	0.0	0.0	0.0	5.6224092385118976	0.0
17	0.0	0.0	0.0	5.915898356878591	0.0
18	0.0	0.0	0.0	6.414299089165701	0.0
19	0.0	0.0	0.0	6.623909313341617	0.0
20	0.0	0.0	0.0	6.833345514915477	0.0
21	0.0	0.0	0.0	7.161900187596365	0.0
22	0.0	0.0	0.0	7.453475057340447	0.0
23	0.0	0.0	0.0	7.814832990508807	0.0
24	0.0	0.0	0.0	8.105798781145696	0.0
25	0.0	0.0	0.0	8.323849101521306	0.0
26	0.0	0.0	0.0	8.561825009832278	0.0
27	0.0	0.0	0.0	8.763691228216722	0.0
28	0.0	0.0	0.0	8.978000062648137	0.0
29	0.0	0.0	0.0	9.230854903434858	0.0
30	0.0	0.0	0.0	9.618577260814634	0.0
31	0.0	0.0	0.0	9.983328634723078	0.0
32	0.0	0.0	0.0	10.246189775127993	0.0
33	0.0	0.0	0.0	10.506614599104132	0.0
34	0.0	0.0	0.0	10.758599326880574	0.0
35	0.0	0.0	0.0	11.179560001252963	0.0
36	0.0	0.0	0.0	11.457561108036712	0.0
37	0.0	0.0	0.0	11.735040147014294	0.0
38	0.0	0.0	0.0	11.993289688464738	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGCGA	95	0.0	44.000004	1
TCACGAC	85	0.0	44.0	25
GTTTGCG	50	2.7284841E-11	44.0	1
AACCGCT	20	7.8564824E-4	44.0	35
AAATCGT	55	1.8189894E-12	44.0	12
CGACGTC	35	1.4460784E-7	44.0	18
CTATCGA	20	7.8564824E-4	44.0	30
GCGAAGA	30	2.5277168E-6	44.0	9
TAGACCG	20	7.8564824E-4	44.0	13
GCTATGT	20	7.8564824E-4	44.0	32
ATTAACG	30	2.5277168E-6	44.0	1
TTCCGTA	20	7.8564824E-4	44.0	40
ATTCGCA	20	7.8564824E-4	44.0	44
TCTAGCG	20	7.8564824E-4	44.0	1
CGTTCAA	25	4.4428405E-5	44.0	9
ATCGTTA	20	7.8564824E-4	44.0	15
TCGGCAC	20	7.8564824E-4	44.0	42
ATCCGTT	20	7.8564824E-4	44.0	34
ATACTAC	25	4.4428405E-5	44.0	44
TACGCAT	45	4.802132E-10	44.0	11
>>END_MODULE