FastQCFastQC Report
Thu 26 May 2016
SRR1546263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546263_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1540933
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139880.9077617261749863No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA38830.2519901903586983No Hit
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG34280.2224626249162034Illumina PCR Primer Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC33270.21590815434545177Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG22630.14685907823377137Illumina PCR Primer Index 10 (95% over 22bp)
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA21160.1373194032446576No Hit
TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG20110.13050534968100494Illumina PCR Primer Index 10 (95% over 22bp)
GGTTAGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18660.12109546618834174No Hit
AGTTTTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA18610.12077098744721541No Hit
TCTGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT18070.11726661704305119No Hit
GAAGGGGGGACATAAGTAATTTAAATTAGGGTTGGTATTTATTTCACTGT17190.111555791199228No Hit
GAGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC17160.1113611039545522No Hit
GATGCAGGGCCAGCAATACTAACTATAATCTCACTGATATTACTATCCCT17060.11071214647229957No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC16670.10818121229151431No Hit
TATGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA15680.1017565332172132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAC302.5282807E-644.013
AGCGTTG302.5282807E-644.01
TGTCAAC750.044.033
CGGCGTA750.044.021
ACTATCG800.044.01
CTGTCGG254.443548E-544.02
ATCTCGA408.3164196E-944.017
GTCGATT454.802132E-1044.09
ATTAGGT1450.044.013
GGTGTCG302.5282807E-644.017
CACGGAT254.443548E-544.030
CATGCGT254.443548E-544.037
TAGACCG254.443548E-544.034
CGCATCG254.443548E-544.021
GCAAATC207.8573177E-444.02
CATCTCG207.8573177E-444.026
CGATTCG207.8573177E-444.02
CGATTAC207.8573177E-444.010
TACGGGT850.044.04
TTATCGT254.443548E-544.041
Produced by FastQC (version 0.11.5)