FastQCFastQC Report
Thu 26 May 2016
SRR1546262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546262_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1585104
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142440.8986161160403355No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA39410.24862721941273253No Hit
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG34750.2192285174979055Illumina PCR Primer Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC34380.2168942858008055Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG23600.14888612986908115Illumina PCR Primer Index 10 (95% over 22bp)
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA22250.1403693385418244No Hit
TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG21600.13626866123610817Illumina PCR Primer Index 10 (95% over 22bp)
GGTTAGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18890.11917199123842978No Hit
AGTTTTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA18000.11355721769675681No Hit
GAGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC17920.11305251895143789No Hit
GAAGGGGGGACATAAGTAATTTAAATTAGGGTTGGTATTTATTTCACTGT17370.10958271507737032No Hit
TCTGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT16630.10491425168317031No Hit
GATGCAGGGCCAGCAATACTAACTATAATCTCACTGATATTACTATCCCT16560.10447264028101626No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC16300.10283236935872976No Hit
TATGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA16090.1015075351522676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG1450.044.0000041
TCGTCAG551.8189894E-1244.0000041
TCGACTG1100.044.0000041
ACGCGAG302.5283261E-644.01
TCGCCAG351.4465513E-744.025
CGGAATA254.4436063E-544.06
CGGCGTA800.044.021
CGGGTAC700.044.06
GTACCTC207.8573875E-444.09
ATCTCGA351.4465513E-744.017
CTCACGA900.044.024
GTACCCG207.8573875E-444.028
TCCGCAC207.8573875E-444.027
CGTGCAT207.8573875E-444.035
CGTGATA207.8573875E-444.039
CGCATTA254.4436063E-544.026
CGTTGTA254.4436063E-544.014
GACGTAC207.8573875E-444.028
TAGCGGA1250.044.03
ATTCGTC207.8573875E-444.014
Produced by FastQC (version 0.11.5)