##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546262_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1585104
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.747465781425067	31.0	31.0	34.0	30.0	34.0
2	32.1495523322129	33.0	31.0	34.0	30.0	34.0
3	32.22751440914918	33.0	31.0	34.0	30.0	34.0
4	35.911634820175834	37.0	35.0	37.0	35.0	37.0
5	35.81181739494696	37.0	35.0	37.0	35.0	37.0
6	35.908824909911274	37.0	35.0	37.0	35.0	37.0
7	36.129014878519015	37.0	35.0	37.0	35.0	37.0
8	36.0286605799998	37.0	35.0	37.0	35.0	37.0
9	37.79613198881587	39.0	38.0	39.0	35.0	39.0
10	37.44620983859734	39.0	37.0	39.0	35.0	39.0
11	37.26169134643531	39.0	37.0	39.0	34.0	39.0
12	36.67994402890914	39.0	35.0	39.0	33.0	39.0
13	36.400623555299845	39.0	35.0	39.0	33.0	39.0
14	37.52224964418738	40.0	35.0	41.0	33.0	41.0
15	37.77529550111539	40.0	35.0	41.0	33.0	41.0
16	37.91638088100213	40.0	35.0	41.0	34.0	41.0
17	37.86823135895184	40.0	35.0	41.0	34.0	41.0
18	37.755855136319134	39.0	36.0	41.0	34.0	41.0
19	37.57060167660923	39.0	36.0	41.0	34.0	41.0
20	37.35882061997194	39.0	35.0	41.0	33.0	41.0
21	37.252085667565034	39.0	35.0	41.0	33.0	41.0
22	37.19147134825223	39.0	35.0	41.0	33.0	41.0
23	37.13608255357377	38.0	35.0	41.0	33.0	41.0
24	37.05145908407272	38.0	35.0	41.0	33.0	41.0
25	37.05525000252349	38.0	35.0	41.0	33.0	41.0
26	37.01145098365785	38.0	35.0	41.0	33.0	41.0
27	36.951280168367504	38.0	35.0	41.0	33.0	41.0
28	36.91069292614239	38.0	35.0	41.0	33.0	41.0
29	36.80906426329124	38.0	35.0	41.0	32.0	41.0
30	36.62613178693638	38.0	35.0	41.0	32.0	41.0
31	36.38645035278442	38.0	35.0	41.0	31.0	41.0
32	36.09296929412834	38.0	35.0	41.0	30.0	41.0
33	35.79462609393453	38.0	35.0	41.0	30.0	41.0
34	35.55191772905753	38.0	35.0	41.0	27.0	41.0
35	35.338189166136736	38.0	35.0	41.0	25.0	41.0
36	35.13493373305474	38.0	35.0	41.0	24.0	41.0
37	35.071950484006095	38.0	35.0	40.0	24.0	41.0
38	35.067319873017794	38.0	35.0	41.0	24.0	41.0
39	35.018512349978295	38.0	35.0	41.0	24.0	41.0
40	34.92438603397632	38.0	35.0	41.0	23.0	41.0
41	34.89050497632963	38.0	34.0	41.0	23.0	41.0
42	34.800164531790976	38.0	34.0	41.0	23.0	41.0
43	34.6858773935338	38.0	34.0	40.0	23.0	41.0
44	34.53391133957141	38.0	34.0	40.0	22.0	41.0
45	34.44151172415185	38.0	34.0	40.0	22.0	41.0
46	34.32493009922377	37.0	34.0	40.0	21.0	41.0
47	34.314800795405226	37.0	34.0	40.0	21.0	41.0
48	34.29117584713684	37.0	34.0	40.0	21.0	41.0
49	34.21497138358114	37.0	34.0	40.0	20.0	41.0
50	34.01062643208269	37.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	5.0
12	3.0
13	3.0
14	13.0
15	21.0
16	48.0
17	141.0
18	399.0
19	833.0
20	1639.0
21	2718.0
22	4626.0
23	7184.0
24	11725.0
25	19802.0
26	30214.0
27	36443.0
28	36007.0
29	32841.0
30	33477.0
31	37826.0
32	46755.0
33	61207.0
34	110848.0
35	222724.0
36	86812.0
37	111508.0
38	192267.0
39	497012.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.275557944462946	24.784682897778314	29.30299841524594	17.636760742512795
2	32.007742078753196	24.711880103766063	31.577423311025647	11.702954506455097
3	25.173553281046544	25.01463626361425	38.71777498511138	11.094035470227821
4	22.779704044655745	27.566771644005694	37.794933329295745	11.858590982042818
5	19.35431366017624	31.071210469470774	36.8254070395381	12.749068830814887
6	16.241710323108137	41.45898313296793	32.68126255438129	9.618043989542642
7	82.15214900725756	3.0225776983718418	12.958077198720083	1.867196095650506
8	82.10723081892418	2.2460986786986847	12.226074755978157	3.4205957463989742
9	78.09310934803017	3.8880729592506236	14.562451422745761	3.456366269973453
10	39.21345224035773	30.55219089725343	20.295702994882355	9.938653867506485
11	31.508658106975947	18.742429518820217	32.700819630762396	17.04809274344144
12	33.26564061411743	18.56572187061543	34.363234210499755	13.805403304767383
13	16.332682271951874	39.01119421817118	29.85141668937811	14.804706820498845
14	9.630724545518781	39.722377837668695	31.595529378513966	19.05136823829856
15	7.890775621031806	26.78669664577214	50.712950065106135	14.609577668089917
16	9.040984061613623	21.151735154286406	45.55051277392524	24.256768010174724
17	9.32468784382602	23.72021015655755	32.59748256265835	34.35761943695808
18	12.366885705922135	26.58418627421292	42.59159020480675	18.457337815058192
19	15.869368823749106	30.720507928817288	32.152085919914406	21.2580373275192
20	21.752957534647567	22.36648194692588	37.65847540603014	18.22208511239641
21	14.152320604830976	29.955889329659126	35.75298529307856	20.138804772431335
22	17.841289909053287	22.792889299377205	29.250825182448597	30.114995609120914
23	12.685413701561538	27.346344466987656	30.95809486317617	29.010146968274636
24	10.714565101091159	23.354114304171837	50.35076562799665	15.580554966740353
25	12.009243557520517	31.85078076895901	38.58819358225076	17.55178209126972
26	15.044501811868496	28.894129344194454	34.340901291019385	21.720467552917665
27	12.03561406696343	35.34613501700834	29.68234260969627	22.93590830633195
28	10.428842523897487	28.13910002119735	39.229097901462616	22.20295955344255
29	10.555017210227216	21.606657985848248	38.531793497461365	29.306531306463175
30	12.886725413600622	32.1639463404294	35.59249109206715	19.356837153902838
31	18.351414165884382	35.072209772986504	28.619636314084122	17.95673974704499
32	17.920401437382026	25.26585006409674	32.909827998667595	23.903920499853637
33	17.5094504840061	31.68063420444337	30.277130081054622	20.532785230495918
34	14.7128516488508	22.715228779941256	34.205074241185436	28.36684533002251
35	15.582195237662638	25.49353228557874	35.314969869484905	23.60930260727372
36	21.429887250300293	29.63691972261757	30.789651656925983	18.143541370156154
37	13.862245000958929	31.6457469036732	36.236486691094086	18.25552140427379
38	13.155098971423957	26.453847823234312	41.04664425804238	19.344408947299357
39	13.470535687248281	31.455790913403792	34.49634850457762	20.57732489477031
40	17.32403678244456	25.026370509442913	36.640687298751374	21.00890540936115
41	11.78130898666586	27.051158788319253	35.78143768484592	25.386094540168973
42	15.137240206320849	26.45618205493141	32.1673530569603	26.239224681787444
43	16.13736385751345	24.21923104099163	34.96723243396017	24.67617266753475
44	14.78533900614723	26.60815946461557	33.981051085607	24.625450443630196
45	13.844580544872764	35.727876530498946	28.73533850144849	21.692204423179803
46	18.23792003553079	31.393965317102225	33.24652514913848	17.121589498228506
47	14.606044776872684	28.048001897667284	34.85165642128214	22.494296904177897
48	14.32057454905167	23.7361081670351	38.09264250169074	23.85067478222249
49	16.502639574438017	24.32149562426188	37.26840636324178	21.90745843805832
50	15.379874128132917	27.79167802238844	35.331246404021435	21.497201445457208
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1938.0
1	3185.0
2	4432.0
3	14740.5
4	25049.0
5	18680.0
6	12311.0
7	13396.0
8	14481.0
9	15638.5
10	16796.0
11	16486.5
12	16177.0
13	15118.5
14	14060.0
15	12854.0
16	11648.0
17	10994.5
18	10341.0
19	10358.0
20	10375.0
21	9492.5
22	8610.0
23	10906.5
24	13203.0
25	14215.0
26	15227.0
27	22708.0
28	30189.0
29	36835.5
30	43482.0
31	55827.5
32	68173.0
33	83007.0
34	97841.0
35	98932.5
36	100024.0
37	107375.5
38	114727.0
39	113618.5
40	112510.0
41	120427.0
42	128344.0
43	131849.5
44	135355.0
45	135342.5
46	135330.0
47	138362.5
48	141395.0
49	128006.5
50	114618.0
51	90600.0
52	66582.0
53	52053.5
54	37525.0
55	29581.0
56	21637.0
57	18576.5
58	15516.0
59	13698.5
60	11881.0
61	11542.5
62	11204.0
63	9392.0
64	7580.0
65	6356.0
66	5132.0
67	4811.0
68	4490.0
69	3638.0
70	2786.0
71	2475.5
72	2165.0
73	1471.0
74	777.0
75	705.0
76	633.0
77	490.5
78	348.0
79	216.5
80	85.0
81	86.0
82	87.0
83	54.0
84	21.0
85	14.0
86	7.0
87	7.0
88	7.0
89	5.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1585104.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.875292587821985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.06365873860967	15.461005462930752
2	10.264867442043734	4.285642224557415
3	4.343557422911842	2.7201909622587217
4	2.4823067835828954	2.0727552160011298
5	1.5539147284686043	1.6219212306654032
6	1.0396515627728924	1.30218183373623
7	0.7307741809027681	1.0678587389379866
8	0.5810559177279848	0.9703769833965681
9	0.43490589730237567	0.8170909068920716
>10	3.172443343151837	14.271425164354252
>50	0.6650627451023484	9.637159270404956
>100	0.5445289032591765	24.21429671911368
>500	0.10531414227332937	15.299590629766085
>1k	0.01765381575680088	5.353449322520181
>5k	0.0	0.0
>10k+	3.043761337379462E-4	0.9050553344645649
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14244	0.8986161160403355	No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	3941	0.24862721941273253	No Hit
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG	3475	0.2192285174979055	Illumina PCR Primer Index 10 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	3438	0.2168942858008055	Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG	2360	0.14888612986908115	Illumina PCR Primer Index 10 (95% over 22bp)
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	2225	0.1403693385418244	No Hit
TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG	2160	0.13626866123610817	Illumina PCR Primer Index 10 (95% over 22bp)
GGTTAGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1889	0.11917199123842978	No Hit
AGTTTTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1800	0.11355721769675681	No Hit
GAGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC	1792	0.11305251895143789	No Hit
GAAGGGGGGACATAAGTAATTTAAATTAGGGTTGGTATTTATTTCACTGT	1737	0.10958271507737032	No Hit
TCTGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1663	0.10491425168317031	No Hit
GATGCAGGGCCAGCAATACTAACTATAATCTCACTGATATTACTATCCCT	1656	0.10447264028101626	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	1630	0.10283236935872976	No Hit
TATGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1609	0.1015075351522676	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24029969011497038	0.0
2	0.0	0.0	0.0	0.9141986898020572	0.0
3	0.0	0.0	0.0	1.2650274051418708	0.0
4	0.0	0.0	0.0	1.9188646297025305	0.0
5	0.0	0.0	0.0	3.2248988079015635	0.0
6	0.0	0.0	0.0	4.372962278815775	0.0
7	0.0	0.0	0.0	5.033549849095075	0.0
8	0.0	0.0	0.0	6.4791332303747895	0.0
9	0.0	0.0	0.0	7.009571611704973	0.0
10	0.0	0.0	0.0	8.107606819489448	0.0
11	0.0	0.0	0.0	9.313458296742674	0.0
12	0.0	0.0	0.0	10.306389990814482	0.0
13	0.0	0.0	0.0	10.661382470803177	0.0
14	0.0	0.0	0.0	10.766738333888503	0.0
15	0.0	0.0	0.0	10.959785603972989	0.0
16	0.0	0.0	0.0	11.467954153165975	0.0
17	0.0	0.0	0.0	12.09188797706649	0.0
18	0.0	0.0	0.0	13.063874673207563	0.0
19	0.0	0.0	0.0	13.430159787622769	0.0
20	0.0	0.0	0.0	13.783575084032341	0.0
21	0.0	0.0	0.0	14.37930886553816	0.0
22	0.0	0.0	0.0	14.927033178895517	0.0
23	0.0	0.0	0.0	15.657332263371993	0.0
24	0.0	0.0	0.0	16.161652484631922	0.0
25	0.0	0.0	0.0	16.54818863620305	0.0
26	0.0	0.0	0.0	16.937563718216598	0.0
27	0.0	0.0	0.0	17.27451321806014	0.0
28	0.0	0.0	0.0	17.62805468915604	0.0
29	0.0	0.0	0.0	18.031624423381682	0.0
30	0.0	0.0	0.0	18.628935388466623	0.0
31	0.0	0.0	0.0	19.094709243052822	0.0
32	0.0	0.0	0.0	19.45689368016231	0.0
33	0.0	0.0	0.0	19.82557611361778	0.0
34	0.0	0.0	0.0	20.182208864528764	0.0
35	0.0	0.0	0.0	20.864498480856778	0.0
36	0.0	0.0	0.0	21.221509755826748	0.0
37	0.0	0.0	0.0	21.563695505152975	0.0
38	0.0	0.0	0.0	21.91092824193239	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTTAG	145	0.0	44.000004	1
TCGTCAG	55	1.8189894E-12	44.000004	1
TCGACTG	110	0.0	44.000004	1
ACGCGAG	30	2.5283261E-6	44.0	1
TCGCCAG	35	1.4465513E-7	44.0	25
CGGAATA	25	4.4436063E-5	44.0	6
CGGCGTA	80	0.0	44.0	21
CGGGTAC	70	0.0	44.0	6
GTACCTC	20	7.8573875E-4	44.0	9
ATCTCGA	35	1.4465513E-7	44.0	17
CTCACGA	90	0.0	44.0	24
GTACCCG	20	7.8573875E-4	44.0	28
TCCGCAC	20	7.8573875E-4	44.0	27
CGTGCAT	20	7.8573875E-4	44.0	35
CGTGATA	20	7.8573875E-4	44.0	39
CGCATTA	25	4.4436063E-5	44.0	26
CGTTGTA	25	4.4436063E-5	44.0	14
GACGTAC	20	7.8573875E-4	44.0	28
TAGCGGA	125	0.0	44.0	3
ATTCGTC	20	7.8573875E-4	44.0	14
>>END_MODULE