FastQCFastQC Report
Thu 26 May 2016
SRR1546261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546261_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1219678
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146931.2046622141253676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22360.18332707485090327No Hit
TAGACAGGGATGAATATAAAATATTTTTATTTCTGTACTTTCCCCCTGTG17490.14339850353945877No Hit
GTGAAGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC17000.13938104975247564No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC16840.13806922810774647TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG16020.13134614217850943TruSeq Adapter, Index 13 (95% over 21bp)
TGCTACGGGAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAG15110.1238851565741122No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGC15080.12363919001572546No Hit
TGTTAAGGGGTTTGATTGTTTACAGAATATTCTAAAATAAAAGGACTCTG15070.1235572011629299No Hit
AGAGCAGGGTATTTTTGGTTATTTAAAAAAAAATTATTTAGAGCTAAAAA14730.12076958016788038No Hit
TTGATGGGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT14320.11740803720326183No Hit
GGTAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT14040.11511234932498578No Hit
ATTTAAGGGAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCA13620.11166881750757167No Hit
TGGAGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13510.11076694012682035No Hit
GTATTGGGGCTTCTTTGTAGCTGCTGTTATTAAAACTCTTGAAACCCATA13480.11052097356843364No Hit
TGAACAGGGCCATATTGACTTTAACAGAAAAAAATCAATTTGTAGATAAT13370.10961909618768231No Hit
AATGTTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA13290.10896318536531774No Hit
TATATAGGGCGATAAATCCATAAATAAAAATATATCAACAATAATTTTAA13100.10740539716220182No Hit
GAATTAGGGATTTATTTCCTATTTTATACCCTAATCGGTTCTATTCCACT13080.10724141945661067No Hit
AGGTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATTG13070.10715943060381511No Hit
ACGATTGGGTATATCATCTCGCTAATATTAACAACAAAACTAACACATAC13060.10707744175101953No Hit
TTTCTAGGGTGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTA13060.10707744175101953No Hit
AAGGAAGGAGGAGGAGAAGGAAGAATTGATGGAGTGGTGGAAAATGATGT12800.1049457315783346No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.10387987649199215No Hit
TAGTAGGGGACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA12410.10174816631930722No Hit
GGAAGAGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA12360.10133822205532937No Hit
TTTACAGGGCCAGTACATAAGTAAAAGGCCATAATGGTCATGTTTTTTTT12350.10125623320253378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAA454.802132E-1044.00000418
CGCATGA650.044.00000439
GTAATCT650.044.0000049
GTCAAAT454.802132E-1044.00000421
TGCTATA650.044.00000429
TATAGCG1300.044.0000041
TGTCGAG454.802132E-1044.0000041
AGAATAC454.802132E-1044.00000443
TAGTAGT454.802132E-1044.00000444
GTCGGAT650.044.0000049
CACCACG454.802132E-1044.00000413
CGGACAA302.5278441E-644.011
GTGTTAC800.044.025
CTAGACG254.4430017E-544.01
CTATGCG207.856672E-444.01
AAGTACG408.312782E-944.01
CGGCGGA207.856672E-444.025
TACCCGG207.856672E-444.033
TACCCGA351.4461875E-744.019
CCCTCAA254.4430017E-544.020