SRR1546261_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1546261_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1219678
Sequences flagged as poor quality	0
Sequence length	50
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14693	1.2046622141253676	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2236	0.18332707485090327	No Hit
TAGACAGGGATGAATATAAAATATTTTTATTTCTGTACTTTCCCCCTGTG	1749	0.14339850353945877	No Hit
GTGAAGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1700	0.13938104975247564	No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	1684	0.13806922810774647	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG	1602	0.13134614217850943	TruSeq Adapter, Index 13 (95% over 21bp)
TGCTACGGGAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAG	1511	0.1238851565741122	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGC	1508	0.12363919001572546	No Hit
TGTTAAGGGGTTTGATTGTTTACAGAATATTCTAAAATAAAAGGACTCTG	1507	0.1235572011629299	No Hit
AGAGCAGGGTATTTTTGGTTATTTAAAAAAAAATTATTTAGAGCTAAAAA	1473	0.12076958016788038	No Hit
TTGATGGGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT	1432	0.11740803720326183	No Hit
GGTAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1404	0.11511234932498578	No Hit
ATTTAAGGGAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCA	1362	0.11166881750757167	No Hit
TGGAGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1351	0.11076694012682035	No Hit
GTATTGGGGCTTCTTTGTAGCTGCTGTTATTAAAACTCTTGAAACCCATA	1348	0.11052097356843364	No Hit
TGAACAGGGCCATATTGACTTTAACAGAAAAAAATCAATTTGTAGATAAT	1337	0.10961909618768231	No Hit
AATGTTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1329	0.10896318536531774	No Hit
TATATAGGGCGATAAATCCATAAATAAAAATATATCAACAATAATTTTAA	1310	0.10740539716220182	No Hit
GAATTAGGGATTTATTTCCTATTTTATACCCTAATCGGTTCTATTCCACT	1308	0.10724141945661067	No Hit
AGGTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATTG	1307	0.10715943060381511	No Hit
ACGATTGGGTATATCATCTCGCTAATATTAACAACAAAACTAACACATAC	1306	0.10707744175101953	No Hit
TTTCTAGGGTGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTA	1306	0.10707744175101953	No Hit
AAGGAAGGAGGAGGAGAAGGAAGAATTGATGGAGTGGTGGAAAATGATGT	1280	0.1049457315783346	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1267	0.10387987649199215	No Hit
TAGTAGGGGACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA	1241	0.10174816631930722	No Hit
GGAAGAGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA	1236	0.10133822205532937	No Hit
TTTACAGGGCCAGTACATAAGTAAAAGGCCATAATGGTCATGTTTTTTTT	1235	0.10125623320253378	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGAAA	45	4.802132E-10	44.000004	18
CGCATGA	65	0.0	44.000004	39
GTAATCT	65	0.0	44.000004	9
GTCAAAT	45	4.802132E-10	44.000004	21
TGCTATA	65	0.0	44.000004	29
TATAGCG	130	0.0	44.000004	1
TGTCGAG	45	4.802132E-10	44.000004	1
AGAATAC	45	4.802132E-10	44.000004	43
TAGTAGT	45	4.802132E-10	44.000004	44
GTCGGAT	65	0.0	44.000004	9
CACCACG	45	4.802132E-10	44.000004	13
CGGACAA	30	2.5278441E-6	44.0	11
GTGTTAC	80	0.0	44.0	25
CTAGACG	25	4.4430017E-5	44.0	1
CTATGCG	20	7.856672E-4	44.0	1
AAGTACG	40	8.312782E-9	44.0	1
CGGCGGA	20	7.856672E-4	44.0	25
TACCCGG	20	7.856672E-4	44.0	33
TACCCGA	35	1.4461875E-7	44.0	19
CCCTCAA	25	4.4430017E-5	44.0	20

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content