##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546261_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1219678
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.874615267308258	31.0	31.0	34.0	30.0	34.0
2	32.23276389342105	33.0	31.0	34.0	30.0	34.0
3	32.13957044400243	33.0	31.0	34.0	30.0	34.0
4	35.88418254654097	37.0	35.0	37.0	35.0	37.0
5	35.85938091857031	37.0	35.0	37.0	35.0	37.0
6	35.938165646998634	37.0	35.0	37.0	35.0	37.0
7	36.18209642217044	37.0	35.0	37.0	35.0	37.0
8	36.07761638727599	37.0	35.0	37.0	35.0	37.0
9	37.86515621336123	39.0	38.0	39.0	35.0	39.0
10	37.49312933413573	39.0	37.0	39.0	35.0	39.0
11	37.29084725640702	39.0	37.0	39.0	34.0	39.0
12	36.51766203866922	39.0	35.0	39.0	33.0	39.0
13	36.201689298323	39.0	35.0	39.0	33.0	39.0
14	37.27730843714489	40.0	35.0	41.0	33.0	41.0
15	37.55372975490253	40.0	35.0	41.0	33.0	41.0
16	37.71914144552907	40.0	35.0	41.0	33.0	41.0
17	37.643888796879175	39.0	35.0	41.0	33.0	41.0
18	37.463829797700704	39.0	35.0	41.0	33.0	41.0
19	37.1896295579653	37.0	35.0	41.0	33.0	41.0
20	36.89059571460664	37.0	35.0	41.0	33.0	41.0
21	36.754979592974536	37.0	35.0	41.0	33.0	41.0
22	36.68299174044297	37.0	35.0	41.0	33.0	41.0
23	36.65003222161915	37.0	35.0	41.0	33.0	41.0
24	36.5599494292756	36.0	35.0	41.0	33.0	41.0
25	36.55161034305776	36.0	35.0	40.0	33.0	41.0
26	36.486484957505176	36.0	35.0	41.0	33.0	41.0
27	36.41414291312953	36.0	35.0	41.0	32.0	41.0
28	36.345028769888444	36.0	35.0	41.0	32.0	41.0
29	36.2410915011995	36.0	35.0	41.0	32.0	41.0
30	36.002631842174736	36.0	35.0	40.0	31.0	41.0
31	35.623720358979995	36.0	35.0	40.0	30.0	41.0
32	35.224572387138245	36.0	35.0	41.0	29.0	41.0
33	34.77484385222985	36.0	34.0	41.0	24.0	41.0
34	34.253841587697735	36.0	34.0	40.0	21.0	41.0
35	34.009491029599616	36.0	34.0	40.0	18.0	41.0
36	33.80417372454041	36.0	34.0	40.0	18.0	41.0
37	33.75028245159788	36.0	34.0	40.0	18.0	41.0
38	33.718368290647206	36.0	33.0	40.0	18.0	41.0
39	33.66018654103788	36.0	34.0	40.0	17.0	41.0
40	33.50193165737186	36.0	33.0	40.0	16.0	41.0
41	33.43658326213968	36.0	34.0	40.0	15.0	41.0
42	33.33241396499732	35.0	33.0	40.0	15.0	41.0
43	33.175001106849514	35.0	33.0	40.0	12.0	41.0
44	33.00211613229065	35.0	33.0	40.0	10.0	41.0
45	32.96062239377934	35.0	33.0	40.0	10.0	41.0
46	32.88762115902722	35.0	33.0	40.0	10.0	41.0
47	32.84847968070261	35.0	33.0	40.0	10.0	41.0
48	32.81173801609933	35.0	33.0	40.0	10.0	41.0
49	32.70339466646115	35.0	33.0	40.0	10.0	41.0
50	32.553375563058445	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	5.0
14	8.0
15	23.0
16	70.0
17	218.0
18	511.0
19	1073.0
20	1970.0
21	3253.0
22	5387.0
23	8467.0
24	13264.0
25	21756.0
26	32492.0
27	38108.0
28	35673.0
29	30509.0
30	29584.0
31	30950.0
32	37051.0
33	48574.0
34	93048.0
35	202941.0
36	58540.0
37	73092.0
38	125830.0
39	327276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.23194154522751	23.21694742382826	28.88434488446951	20.666766146474725
2	35.203799691393954	25.083423657719493	28.5584391946071	11.154337456279444
3	24.65044052610607	23.71052031765761	41.36444209045338	10.274597065782936
4	22.758055814731428	26.845856037413153	39.153940630231915	11.242147517623504
5	19.29902810413896	28.874014289017264	39.713268584003316	12.113689022840454
6	16.784020044634733	40.548571016284626	34.164672971062856	8.502735968017788
7	78.82088551240574	3.4819845893752284	16.186567274313386	1.5105626239056538
8	78.95715098575198	2.8163990823807596	15.60149482076417	2.6249551111030947
9	76.0949201346585	3.8525742040112227	17.549631952039803	2.502873709290485
10	43.17524789329642	23.41568840300473	23.025995385667365	10.38306831803148
11	37.16021769680194	19.320017250454626	30.866097445391325	12.653667607352103
12	35.89316196569914	19.67142147353646	33.12849784943239	11.306918711332008
13	15.701111276910792	41.276386062550934	30.76582507842234	12.256677582115936
14	9.295650163403783	41.34886420842222	34.73949681801262	14.61598881016137
15	8.430257822146501	25.260027646641163	54.803070974470316	11.506643556742025
16	10.209825872074433	18.370668323934677	51.5874681678279	19.832037636162987
17	9.649268085511094	21.19165878207199	36.199390330890616	32.9596828015263
18	13.476671711714076	24.39315950603356	44.99859799061719	17.13157079163517
19	17.488960200971075	25.91192101521877	35.35228150380674	21.24683728000341
20	21.409749130508217	21.743197794827815	39.942181461008566	16.904871613655406
21	14.86531691151271	28.374046264669854	38.508278414466766	18.252358409350663
22	18.875145735185843	22.938349302028897	32.02746954524063	26.15903541754463
23	12.555608939408597	27.46167431076071	33.180806737515965	26.80191001231473
24	10.954038688899857	22.60014528424715	51.59312539867079	14.852690628182192
25	11.097519181292112	28.06371845683861	43.86428221219043	16.97448014967885
26	12.471570365293134	30.145087473907044	36.66713673608936	20.716205424710456
27	12.092207943408015	37.15874189745163	32.881793391370515	17.867256767769852
28	10.92042325925367	30.291437576147146	41.30213056232875	17.486008602270438
29	11.34078010753658	23.67223152340208	39.57692112180428	25.41006724725706
30	12.931855784887485	33.87336657708018	37.28959610651336	15.905181531518975
31	19.019528104958848	33.857624717343434	32.43683988724893	14.68600729044879
32	20.503198385147556	24.724968393297246	36.728710364538834	18.04312285701636
33	21.667440094844707	32.51907470660289	29.08185603085405	16.731629167698358
34	15.785641784143028	28.446442421688346	32.95804302447039	22.80987276969823
35	17.181092058723696	26.20191558755672	34.218867602760724	22.398124750958857
36	26.05302383087995	27.97902397190078	30.39244784279129	15.575504354427972
37	14.206454490447479	32.54268749620802	37.088641428311405	16.1622165850331
38	14.319271151894188	29.967089674487855	35.10565903459766	20.6079801390203
39	14.5765521719667	30.85576684993908	35.66720068739454	18.900480290699676
40	17.986304582029028	27.538989798946933	31.87570817871602	22.598997440308015
41	12.804035163379188	27.35123532604507	34.35365727675665	25.49107223381909
42	17.05302547065701	25.96824735708933	31.5241399779286	25.45458719432506
43	18.051567708854304	24.504582356982745	34.47631260053883	22.967537333624122
44	14.132418556373075	28.239174601821137	33.58345399359503	24.04495284821076
45	14.353460503509943	38.64339604387387	28.4602985378108	18.542844914805386
46	20.165650278188178	31.00588843940778	31.165356758095168	17.663104524308874
47	16.119910336990586	30.00406664709866	32.67805109217351	21.19797192373725
48	16.93635533312891	27.356154657212805	34.609626475184434	21.097863534473856
49	18.956888621423033	26.039823625579867	35.36285806581737	19.64042968717973
50	17.589560523351246	30.121802639713103	32.21858556110711	20.070051275828536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2778.0
1	3804.0
2	4830.0
3	15020.5
4	25211.0
5	19339.5
6	13468.0
7	15110.5
8	16753.0
9	18312.0
10	19871.0
11	19921.5
12	19972.0
13	18762.0
14	17552.0
15	15883.5
16	14215.0
17	13661.5
18	13108.0
19	11431.0
20	9754.0
21	9800.5
22	9847.0
23	9844.5
24	9842.0
25	10724.0
26	11606.0
27	15155.0
28	18704.0
29	25264.0
30	31824.0
31	39213.0
32	46602.0
33	52421.0
34	58240.0
35	67203.0
36	76166.0
37	73136.5
38	70107.0
39	74604.5
40	79102.0
41	89852.0
42	100602.0
43	108740.5
44	116879.0
45	114017.5
46	111156.0
47	100834.5
48	90513.0
49	83172.5
50	75832.0
51	60327.5
52	44823.0
53	37267.5
54	29712.0
55	24999.5
56	20287.0
57	17535.5
58	14784.0
59	13636.5
60	12489.0
61	11438.0
62	10387.0
63	8643.5
64	6900.0
65	6087.0
66	5274.0
67	4432.5
68	3591.0
69	3190.0
70	2789.0
71	2351.0
72	1913.0
73	1518.0
74	1123.0
75	861.5
76	600.0
77	419.0
78	238.0
79	172.0
80	106.0
81	103.5
82	101.0
83	60.0
84	19.0
85	10.5
86	2.0
87	2.0
88	2.0
89	1.5
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1219678.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.071443849135996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.51549159923164	18.114657557189815
2	9.119941654764329	4.208204435905833
3	3.600583242679766	2.4921196232287874
4	1.9013793681588862	1.7547026931353369
5	1.1804435983888801	1.3617269098650548
6	0.7659990397933828	1.0603622301051072
7	0.5081787854324274	0.8207092819398465
8	0.3870557134454225	0.714394732739467
9	0.3086479500438515	0.6408858463728888
>10	2.3838917314563557	12.420229585496271
>50	0.7117126272722903	11.3701904196826
>100	0.5159201920026095	24.836104161320556
>500	0.07645908662642546	12.234066284340154
>1k	0.023938125252198628	6.760488843816524
>5k	0.0	0.0
>10k+	3.572854515253527E-4	1.2111573948618064
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14693	1.2046622141253676	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2236	0.18332707485090327	No Hit
TAGACAGGGATGAATATAAAATATTTTTATTTCTGTACTTTCCCCCTGTG	1749	0.14339850353945877	No Hit
GTGAAGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1700	0.13938104975247564	No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	1684	0.13806922810774647	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG	1602	0.13134614217850943	TruSeq Adapter, Index 13 (95% over 21bp)
TGCTACGGGAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAG	1511	0.1238851565741122	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGC	1508	0.12363919001572546	No Hit
TGTTAAGGGGTTTGATTGTTTACAGAATATTCTAAAATAAAAGGACTCTG	1507	0.1235572011629299	No Hit
AGAGCAGGGTATTTTTGGTTATTTAAAAAAAAATTATTTAGAGCTAAAAA	1473	0.12076958016788038	No Hit
TTGATGGGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT	1432	0.11740803720326183	No Hit
GGTAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1404	0.11511234932498578	No Hit
ATTTAAGGGAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCA	1362	0.11166881750757167	No Hit
TGGAGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1351	0.11076694012682035	No Hit
GTATTGGGGCTTCTTTGTAGCTGCTGTTATTAAAACTCTTGAAACCCATA	1348	0.11052097356843364	No Hit
TGAACAGGGCCATATTGACTTTAACAGAAAAAAATCAATTTGTAGATAAT	1337	0.10961909618768231	No Hit
AATGTTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1329	0.10896318536531774	No Hit
TATATAGGGCGATAAATCCATAAATAAAAATATATCAACAATAATTTTAA	1310	0.10740539716220182	No Hit
GAATTAGGGATTTATTTCCTATTTTATACCCTAATCGGTTCTATTCCACT	1308	0.10724141945661067	No Hit
AGGTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATTG	1307	0.10715943060381511	No Hit
ACGATTGGGTATATCATCTCGCTAATATTAACAACAAAACTAACACATAC	1306	0.10707744175101953	No Hit
TTTCTAGGGTGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTA	1306	0.10707744175101953	No Hit
AAGGAAGGAGGAGGAGAAGGAAGAATTGATGGAGTGGTGGAAAATGATGT	1280	0.1049457315783346	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1267	0.10387987649199215	No Hit
TAGTAGGGGACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA	1241	0.10174816631930722	No Hit
GGAAGAGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA	1236	0.10133822205532937	No Hit
TTTACAGGGCCAGTACATAAGTAAAAGGCCATAATGGTCATGTTTTTTTT	1235	0.10125623320253378	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15758257507309306	0.0
2	0.0	0.0	0.0	0.5780214122087961	0.0
3	0.0	0.0	0.0	0.7996372813152324	0.0
4	0.0	0.0	0.0	1.2433609526448783	0.0
5	0.0	0.0	0.0	1.9951167439274957	0.0
6	0.0	0.0	0.0	2.732196530559705	0.0
7	0.0	0.0	0.0	3.1518154791674524	0.0
8	0.0	0.0	0.0	4.163967866928813	0.0
9	0.0	0.0	0.0	4.534967425828785	0.0
10	0.0	0.0	0.0	5.204816353168623	0.0
11	0.0	0.0	0.0	5.983710454726576	0.0
12	0.0	0.0	0.0	6.634701945923433	0.0
13	0.0	0.0	0.0	6.863532834075879	0.0
14	0.0	0.0	0.0	6.930435737957067	0.0
15	0.0	0.0	0.0	7.053582994856019	0.0
16	0.0	0.0	0.0	7.391787012637762	0.0
17	0.0	0.0	0.0	7.7664760699135345	0.0
18	0.0	0.0	0.0	8.418123471932756	0.0
19	0.0	0.0	0.0	8.652775568633688	0.0
20	0.0	0.0	0.0	8.887181698776235	0.0
21	0.0	0.0	0.0	9.247112762548804	0.0
22	0.0	0.0	0.0	9.557194603821664	0.0
23	0.0	0.0	0.0	9.970664388469743	0.0
24	0.0	0.0	0.0	10.276810764808417	0.0
25	0.0	0.0	0.0	10.499492489001195	0.0
26	8.198885279557392E-5	0.0	0.0	10.779566410150876	0.0
27	8.198885279557392E-5	0.0	0.0	10.988965940190772	0.0
28	8.198885279557392E-5	0.0	0.0	11.21796080604881	0.0
29	8.198885279557392E-5	0.0	0.0	11.49672290555376	0.0
30	1.6397770559114784E-4	0.0	0.0	11.92519665026343	0.0
31	1.6397770559114784E-4	0.0	0.0	12.33530489194689	0.0
32	1.6397770559114784E-4	0.0	0.0	12.639319558112879	0.0
33	1.6397770559114784E-4	0.0	0.0	12.901437920500328	0.0
34	1.6397770559114784E-4	0.0	0.0	13.198073589914715	0.0
35	1.6397770559114784E-4	0.0	0.0	13.643683004858659	0.0
36	1.6397770559114784E-4	0.0	0.0	13.94154850706498	0.0
37	1.6397770559114784E-4	0.0	0.0	14.270241817922436	0.0
38	1.6397770559114784E-4	0.0	0.0	14.546052318726746	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGAAA	45	4.802132E-10	44.000004	18
CGCATGA	65	0.0	44.000004	39
GTAATCT	65	0.0	44.000004	9
GTCAAAT	45	4.802132E-10	44.000004	21
TGCTATA	65	0.0	44.000004	29
TATAGCG	130	0.0	44.000004	1
TGTCGAG	45	4.802132E-10	44.000004	1
AGAATAC	45	4.802132E-10	44.000004	43
TAGTAGT	45	4.802132E-10	44.000004	44
GTCGGAT	65	0.0	44.000004	9
CACCACG	45	4.802132E-10	44.000004	13
CGGACAA	30	2.5278441E-6	44.0	11
GTGTTAC	80	0.0	44.0	25
CTAGACG	25	4.4430017E-5	44.0	1
CTATGCG	20	7.856672E-4	44.0	1
AAGTACG	40	8.312782E-9	44.0	1
CGGCGGA	20	7.856672E-4	44.0	25
TACCCGG	20	7.856672E-4	44.0	33
TACCCGA	35	1.4461875E-7	44.0	19
CCCTCAA	25	4.4430017E-5	44.0	20
>>END_MODULE