FastQCFastQC Report
Thu 26 May 2016
SRR1546260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546260_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1256067
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149011.1863220672145673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23970.19083376921772485No Hit
GTGAAGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18240.14521518358495208No Hit
TAGACAGGGATGAATATAAAATATTTTTATTTCTGTACTTTCCCCCTGTG17690.14083643627290582No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGC16860.13422850851109056No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC16710.13303430469871433TruSeq Adapter, Index 16 (95% over 21bp)
TGTTAAGGGGTTTGATTGTTTACAGAATATTCTAAAATAAAAGGACTCTG15740.12531178671201457No Hit
CCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG15620.12435642366211357TruSeq Adapter, Index 13 (95% over 21bp)
TGCTACGGGAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAG15330.12204762962485281No Hit
AGAGCAGGGTATTTTTGGTTATTTAAAAAAAAATTATTTAGAGCTAAAAA15190.12093303939996831No Hit
GGTAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT14130.11249399912584282No Hit
TTGATGGGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT14060.11193670401340056No Hit
ATTTAAGGGAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCA13910.1107425002010243No Hit
TATATAGGGCGATAAATCCATAAATAAAAATATATCAACAATAATTTTAA13910.1107425002010243No Hit
AATGTTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA13850.11026481867607381No Hit
TTTCTAGGGTGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTA13730.10930945562617281No Hit
AGGTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATTG13580.10811525181379657No Hit
GTATTGGGGCTTCTTTGTAGCTGCTGTTATTAAAACTCTTGAAACCCATA13480.10731911593887906No Hit
TGGAGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13390.1066025936514533No Hit
TGAACAGGGCCATATTGACTTTAACAGAAAAAAATCAATTTGTAGATAAT13380.10652298006396156No Hit
AGTTAGGGGACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA13080.10413457243920905No Hit
GGAAGAGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA13030.10373650450175032No Hit
AAGGAAGGAGGAGGAGAAGGAAGAATTGATGGAGTGGTGGAAAATGATGT12940.10301998221432455No Hit
TTTTTTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA12790.10182577840194831No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.1015073240519813No Hit
AGATCCGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA12750.1015073240519813No Hit
GAATTAGGGATTTATTTCCTATTTTATACCCTAATCGGTTCTATTCCACT12690.10102964252703081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG1450.044.0000042
ATTAACG650.044.0000041
CCGACAT650.044.00000418
TATAGCG1300.044.0000041
TGATCAT302.527906E-644.042
CTTGTAG750.044.01
AACGTAA800.044.022
GACAGGT207.856762E-444.09
ACACGTC254.4430773E-544.020
TACCCGA408.312782E-944.019
GCCCAAT408.312782E-944.010
ATTAGTC700.044.044
CCCTCGA254.4430773E-544.018
GTATTAC502.7284841E-1144.012
GTCGAGG454.802132E-1044.02
GATCGCC207.856762E-444.030
GTTTCGT351.4462239E-744.043
CACGGCA207.856762E-444.037
ACGTTAG502.7284841E-1144.01
GCAACCG254.4430773E-544.01
Produced by FastQC (version 0.11.5)