##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546260_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1256067
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8125521966583	31.0	31.0	34.0	30.0	34.0
2	32.20683530416769	33.0	31.0	34.0	30.0	34.0
3	32.229108001404384	33.0	31.0	34.0	30.0	34.0
4	35.92169287147899	37.0	35.0	37.0	35.0	37.0
5	35.82376656659239	37.0	35.0	37.0	35.0	37.0
6	35.92192932383384	37.0	35.0	37.0	35.0	37.0
7	36.16092374053295	37.0	35.0	37.0	35.0	37.0
8	36.060583551673595	37.0	35.0	37.0	35.0	37.0
9	37.84597636909496	39.0	38.0	39.0	35.0	39.0
10	37.491908473035274	39.0	37.0	39.0	35.0	39.0
11	37.29082525056386	39.0	37.0	39.0	34.0	39.0
12	36.507226923404566	39.0	35.0	39.0	33.0	39.0
13	36.134323248680204	39.0	35.0	39.0	32.0	39.0
14	37.199443182569084	40.0	35.0	41.0	33.0	41.0
15	37.52310585342979	40.0	35.0	41.0	33.0	41.0
16	37.679458977904844	40.0	35.0	41.0	33.0	41.0
17	37.609177695138875	39.0	35.0	41.0	33.0	41.0
18	37.44627794536438	39.0	35.0	41.0	33.0	41.0
19	37.17572311031179	37.0	35.0	41.0	33.0	41.0
20	36.878428459628346	37.0	35.0	41.0	33.0	41.0
21	36.737730550997675	37.0	35.0	41.0	33.0	41.0
22	36.67257160645093	37.0	35.0	41.0	33.0	41.0
23	36.63817296370337	37.0	35.0	41.0	33.0	41.0
24	36.5507675944038	36.0	35.0	41.0	33.0	41.0
25	36.527485396877715	36.0	35.0	40.0	33.0	41.0
26	36.47839645496617	36.0	35.0	40.0	33.0	41.0
27	36.402700652114895	36.0	35.0	41.0	32.0	41.0
28	36.31995028927597	36.0	35.0	41.0	32.0	41.0
29	36.213016503100555	36.0	35.0	41.0	32.0	41.0
30	35.981953988123244	36.0	35.0	40.0	31.0	41.0
31	35.615422584941726	36.0	35.0	40.0	30.0	41.0
32	35.22984761163218	36.0	35.0	41.0	29.0	41.0
33	34.75755035360375	36.0	34.0	40.0	23.0	41.0
34	34.32660916973378	36.0	34.0	40.0	21.0	41.0
35	34.048837362975064	36.0	34.0	40.0	18.0	41.0
36	33.83520385457145	36.0	34.0	40.0	18.0	41.0
37	33.69797391381192	36.0	33.0	40.0	18.0	41.0
38	33.6853511795151	36.0	33.0	40.0	18.0	41.0
39	33.626074086812245	36.0	33.0	40.0	17.0	41.0
40	33.47775636172274	36.0	33.0	40.0	15.0	41.0
41	33.438779937694406	36.0	34.0	40.0	15.0	41.0
42	33.318955915568196	35.0	33.0	40.0	15.0	41.0
43	33.18751069807582	35.0	33.0	40.0	12.0	41.0
44	33.01364576889608	35.0	33.0	40.0	10.0	41.0
45	32.96331246661205	35.0	33.0	40.0	10.0	41.0
46	32.90466830192975	35.0	33.0	40.0	10.0	41.0
47	32.85367261459779	35.0	33.0	40.0	10.0	41.0
48	32.82090286585031	35.0	33.0	40.0	10.0	41.0
49	32.70749808728356	35.0	33.0	40.0	10.0	41.0
50	32.531207332092954	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	3.0
13	2.0
14	10.0
15	28.0
16	73.0
17	245.0
18	569.0
19	1093.0
20	1989.0
21	3539.0
22	5517.0
23	8842.0
24	13588.0
25	22009.0
26	32563.0
27	39425.0
28	37209.0
29	32292.0
30	30694.0
31	32425.0
32	38358.0
33	50438.0
34	98240.0
35	204936.0
36	61078.0
37	75727.0
38	129705.0
39	335467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.33413106148	23.28291404837481	28.89949341874279	20.4834614714024
2	35.24732359022249	24.99317313487258	28.66479256281711	11.09471071208781
3	24.63316049223489	23.716569259442373	41.3578256573893	10.292444590933446
4	22.82083678657269	26.844268657643262	39.08541502961228	11.249479526171772
5	19.223019154233015	29.026556704379626	39.67662553032601	12.073798611061353
6	16.823704467994144	40.40349758412569	34.23185228176522	8.540945666114945
7	78.86649358672746	3.4932849919630082	16.139982978614995	1.5002384426945377
8	78.9472217644441	2.8581277909538265	15.551240499113502	2.643409945488577
9	76.0665633282301	3.870892237436379	17.509575524235572	2.5529689100979485
10	43.20342784262304	23.39094968660111	22.98595536703058	10.419667103745262
11	37.132334501264666	19.411225675063513	30.785698533597333	12.670741290074494
12	35.86982223082049	19.66630760938708	33.110415288356435	11.353454871435998
13	15.7799703359773	41.1860195355821	30.79811825324604	12.235891875194556
14	9.311525579447594	41.35360613725223	34.693053794104934	14.641814489195243
15	8.34238937891052	25.255420292070408	54.77239669539921	11.629793633619864
16	10.293479567570838	18.370516859371357	51.62503274108785	19.710970831969952
17	9.640568536550996	21.23580987319944	36.1881969672	32.93542462304956
18	13.497289555413843	24.372187152436933	44.995211242712365	17.135312049436855
19	17.510292046522995	25.910799344302493	35.30599880420392	21.272909804970595
20	21.374019061085118	21.806480068340303	39.87764983874268	16.941851031831902
21	14.892915744144222	28.33925260356335	38.580426044152105	18.18740560814033
22	18.862289989307897	22.982691209943418	32.01580807393236	26.139210726816327
23	12.59136654334522	27.41732726040888	33.19910482482224	26.79220137142366
24	10.958332636714443	22.689952048736252	51.41533055163458	14.936384762914717
25	11.1334029156088	27.938716644892352	43.92401042301087	17.003870016487973
26	12.436836570023733	30.180396427897556	36.62240947338	20.76035752869871
27	12.152138381153236	37.125726573502845	32.88120776996769	17.840927275376234
28	10.928477541405037	30.32210861363287	41.24732199795075	17.502091847011343
29	11.341433219724744	23.68384807498326	39.57440168398661	25.40031702130539
30	13.009656332026875	33.830042505694365	37.25517826676443	15.905122895514332
31	18.94572502899925	33.800903932672384	32.45981305137386	14.793557986954516
32	20.527487785285338	24.76866281814585	36.65465297631416	18.049196420254653
33	21.628543700296245	32.46642097913567	29.10322458913418	16.80181073143391
34	15.829967668922121	28.50413234325876	32.80844095100022	22.857459036818895
35	17.20847693634177	26.1693842764757	34.06211611323282	22.560022673949717
36	26.12758714304253	27.994207315374098	30.219964380880953	15.658241160702415
37	14.243985392498967	32.51856787894276	37.005350829215324	16.232095899342948
38	14.319697914203623	29.99011995379227	34.954823269777805	20.7353588622263
39	14.665300497505307	30.75464923447555	35.632573740095076	18.947476527924067
40	18.06097923120343	27.467961502053633	31.798064912142426	22.67299435460051
41	12.846607704843771	27.357219001852606	34.20366907179314	25.592504221510477
42	17.10561618130243	25.94200787059926	31.56806125787876	25.38431469021955
43	18.142662771969967	24.417885351657194	34.400553473660246	23.038898402712594
44	14.219305180376523	28.174850545392882	33.43436297586037	24.17148129837023
45	14.46937145868811	38.402967357632996	28.420538076392422	18.707123107286474
46	20.276545757511343	30.893973012586113	31.092131231853077	17.737349998049467
47	16.13616152641539	29.988766522804916	32.561001921075864	21.31407002970383
48	16.978393668490614	27.351009142028254	34.50803181677411	21.16256537270703
49	19.048108102513638	25.81462613061246	35.362046769798106	19.775218997075793
50	17.6955528646163	30.090273846856896	32.023291751156584	20.19088153737022
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2917.0
1	3946.5
2	4976.0
3	15329.0
4	25682.0
5	19602.0
6	13522.0
7	15253.0
8	16984.0
9	18555.0
10	20126.0
11	20218.5
12	20311.0
13	19065.0
14	17819.0
15	16312.0
16	14805.0
17	14088.0
18	13371.0
19	11806.0
20	10241.0
21	10182.5
22	10124.0
23	10184.5
24	10245.0
25	11173.5
26	12102.0
27	15842.5
28	19583.0
29	26184.5
30	32786.0
31	40244.0
32	47702.0
33	53575.0
34	59448.0
35	68492.0
36	77536.0
37	74517.0
38	71498.0
39	76355.5
40	81213.0
41	92226.0
42	103239.0
43	111871.5
44	120504.0
45	117478.5
46	114453.0
47	104641.5
48	94830.0
49	86881.5
50	78933.0
51	63067.5
52	47202.0
53	39251.5
54	31301.0
55	26227.5
56	21154.0
57	18229.0
58	15304.0
59	14049.0
60	12794.0
61	11587.0
62	10380.0
63	8746.5
64	7113.0
65	6236.0
66	5359.0
67	4496.5
68	3634.0
69	3193.0
70	2752.0
71	2379.5
72	2007.0
73	1536.5
74	1066.0
75	825.0
76	584.0
77	422.0
78	260.0
79	180.0
80	100.0
81	89.5
82	79.0
83	48.0
84	17.0
85	11.5
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1256067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.02960473284017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.24328360121692	18.01911894335541
2	9.184710395570377	4.230404999911878
3	3.6287334163206695	2.507048887761413
4	1.9898812488742816	1.8330471450746024
5	1.1993906315808693	1.3810746082789476
6	0.7524811721661483	1.0397606378334388
7	0.5848246446311933	0.942779628271607
8	0.38361025018523265	0.7067513946585469
9	0.31142755760465457	0.6454848199094105
>10	2.400989882786532	12.298525365078877
>50	0.7074630260079514	11.353951347403797
>100	0.5088585962449054	24.253535967858568
>500	0.07965045696519694	12.604171411988819
>1k	0.02434730125573706	6.990756171219564
>5k	0.0	0.0
>10k+	3.4781858936767224E-4	1.1935886713951775
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14901	1.1863220672145673	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2397	0.19083376921772485	No Hit
GTGAAGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1824	0.14521518358495208	No Hit
TAGACAGGGATGAATATAAAATATTTTTATTTCTGTACTTTCCCCCTGTG	1769	0.14083643627290582	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGC	1686	0.13422850851109056	No Hit
CTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTGC	1671	0.13303430469871433	TruSeq Adapter, Index 16 (95% over 21bp)
TGTTAAGGGGTTTGATTGTTTACAGAATATTCTAAAATAAAAGGACTCTG	1574	0.12531178671201457	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTACACTCGTATGCCGTCTTCTG	1562	0.12435642366211357	TruSeq Adapter, Index 13 (95% over 21bp)
TGCTACGGGAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAG	1533	0.12204762962485281	No Hit
AGAGCAGGGTATTTTTGGTTATTTAAAAAAAAATTATTTAGAGCTAAAAA	1519	0.12093303939996831	No Hit
GGTAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1413	0.11249399912584282	No Hit
TTGATGGGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATT	1406	0.11193670401340056	No Hit
ATTTAAGGGAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCA	1391	0.1107425002010243	No Hit
TATATAGGGCGATAAATCCATAAATAAAAATATATCAACAATAATTTTAA	1391	0.1107425002010243	No Hit
AATGTTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1385	0.11026481867607381	No Hit
TTTCTAGGGTGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTA	1373	0.10930945562617281	No Hit
AGGTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATTG	1358	0.10811525181379657	No Hit
GTATTGGGGCTTCTTTGTAGCTGCTGTTATTAAAACTCTTGAAACCCATA	1348	0.10731911593887906	No Hit
TGGAGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1339	0.1066025936514533	No Hit
TGAACAGGGCCATATTGACTTTAACAGAAAAAAATCAATTTGTAGATAAT	1338	0.10652298006396156	No Hit
AGTTAGGGGACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA	1308	0.10413457243920905	No Hit
GGAAGAGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA	1303	0.10373650450175032	No Hit
AAGGAAGGAGGAGGAGAAGGAAGAATTGATGGAGTGGTGGAAAATGATGT	1294	0.10301998221432455	No Hit
TTTTTTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1279	0.10182577840194831	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1275	0.1015073240519813	No Hit
AGATCCGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA	1275	0.1015073240519813	No Hit
GAATTAGGGATTTATTTCCTATTTTATACCCTAATCGGTTCTATTCCACT	1269	0.10102964252703081	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15675915377125582	0.0
2	0.0	0.0	0.0	0.5770392821402043	0.0
3	0.0	0.0	0.0	0.7982854417797777	0.0
4	0.0	0.0	0.0	1.2379116719092214	0.0
5	0.0	0.0	0.0	2.003396315642398	0.0
6	0.0	0.0	0.0	2.74499688312805	0.0
7	0.0	0.0	0.0	3.163366285397196	0.0
8	0.0	0.0	0.0	4.197069105390079	0.0
9	0.0	0.0	0.0	4.584548435712426	0.0
10	0.0	0.0	0.0	5.283078052365041	0.0
11	0.0	0.0	0.0	6.103336844292541	0.0
12	0.0	0.0	0.0	6.77702702164773	0.0
13	0.0	0.0	0.0	7.013479376498228	0.0
14	0.0	0.0	0.0	7.086883104165622	0.0
15	0.0	0.0	0.0	7.21052300554031	0.0
16	0.0	0.0	0.0	7.561220858441469	0.0
17	0.0	0.0	0.0	7.954034299125763	0.0
18	0.0	0.0	0.0	8.64308989886686	0.0
19	0.0	0.0	0.0	8.885354045604256	0.0
20	0.0	0.0	0.0	9.136614527728218	0.0
21	0.0	0.0	0.0	9.520670473788421	0.0
22	0.0	0.0	0.0	9.84270743519255	0.0
23	0.0	0.0	0.0	10.28448323218427	0.0
24	7.961358749175004E-5	0.0	0.0	10.610739713725462	0.0
25	7.961358749175004E-5	0.0	0.0	10.851013520775563	0.0
26	7.961358749175004E-5	0.0	0.0	11.148211042882266	0.0
27	7.961358749175004E-5	0.0	0.0	11.36085893507273	0.0
28	7.961358749175004E-5	0.0	0.0	11.589827612699004	0.0
29	7.961358749175004E-5	0.0	0.0	11.881531797268776	0.0
30	7.961358749175004E-5	0.0	0.0	12.331667020947132	0.0
31	7.961358749175004E-5	0.0	0.0	12.761421166227597	0.0
32	7.961358749175004E-5	0.0	0.0	13.082263923819351	0.0
33	7.961358749175004E-5	0.0	0.0	13.348571373979254	0.0
34	7.961358749175004E-5	0.0	0.0	13.642265898236321	0.0
35	1.592271749835001E-4	0.0	0.0	14.099407117613948	0.0
36	1.592271749835001E-4	0.0	0.0	14.410935085469166	0.0
37	1.592271749835001E-4	0.0	0.0	14.74499369858455	0.0
38	1.592271749835001E-4	0.0	0.0	15.024437390680593	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	145	0.0	44.000004	2
ATTAACG	65	0.0	44.000004	1
CCGACAT	65	0.0	44.000004	18
TATAGCG	130	0.0	44.000004	1
TGATCAT	30	2.527906E-6	44.0	42
CTTGTAG	75	0.0	44.0	1
AACGTAA	80	0.0	44.0	22
GACAGGT	20	7.856762E-4	44.0	9
ACACGTC	25	4.4430773E-5	44.0	20
TACCCGA	40	8.312782E-9	44.0	19
GCCCAAT	40	8.312782E-9	44.0	10
ATTAGTC	70	0.0	44.0	44
CCCTCGA	25	4.4430773E-5	44.0	18
GTATTAC	50	2.7284841E-11	44.0	12
GTCGAGG	45	4.802132E-10	44.0	2
GATCGCC	20	7.856762E-4	44.0	30
GTTTCGT	35	1.4462239E-7	44.0	43
CACGGCA	20	7.856762E-4	44.0	37
ACGTTAG	50	2.7284841E-11	44.0	1
GCAACCG	25	4.4430773E-5	44.0	1
>>END_MODULE