FastQCFastQC Report
Thu 26 May 2016
SRR1546259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546259_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1547771
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234591.515663492855209No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC45060.2911283387529551No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA30170.19492547670165677No Hit
GTAAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT27800.17961313398429096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26360.17030943208006868No Hit
ACTTAGGGGATTTTTGAAAGATTCAACTTGTACTGCTGGTATTGTGTAGT22790.14724400444251767No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC21500.13890943815331855No Hit
TGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA21170.1367773398002676No Hit
GAATTAGGGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGC18950.12243413269792496No Hit
CTCACTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT18650.12049586146787865No Hit
GATAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT18390.11881602640183853No Hit
TTAGCTGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC18190.11752384558180766No Hit
TTAAATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT18010.11636088284377986No Hit
GTCGACGGGGGTTTTTAATATTCGATAAATCCATAAATAAAAATATATCA17360.11216129517867954No Hit
AGCTATGGGACAACCTCAATCTTATTAATCTTCATTCTTCTACTATCCCC17330.1119674680556749No Hit
ATAGATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG17220.11125676860465793No Hit
TAGTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT17220.11125676860465793No Hit
TAAGTTGGGATGATATTAAAATCCCAACTATACCAAAGATATCCCAATTA16620.10738022614456531No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGC16510.10666952669354833No Hit
AAGCACGGGATCAACAATAATTTTAAATAATCTAAGTATAGGTAATATAA16450.10628187244753907No Hit
TTTCTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT16420.10608804532453445No Hit
GACATCGGGATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATT16330.10550656395552055No Hit
AATACGGGGAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCAT16120.10414977409448814No Hit
GGAACTGGGAATCTAAGTATAGGTAATATAACAATTAAAAAGATTTAGAG16090.10395594697148351No Hit
TTACGAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA15910.10279298423345573No Hit
GAGATTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC15860.102469939028448No Hit
TGGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT15810.10214689382344029No Hit
TGGAGAGTTGTATTTTGTTTAAAGAACATTTTCTGATTTTCGAATGTATT15510.10020862259339398No Hit
GTTCTAGGGAAGTCTAGTGGAACAGTCAGTTTAACTTTTTAACAGATCTT15490.10007940451139088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG800.044.00000419
AGCCGAA207.857327E-444.00000437
TCCGCGT207.857327E-444.00000423
CGTATGG551.8189894E-1244.0000042
GGTCGTA207.857327E-444.0000048
ACGTTGG650.044.0000042
TAGCGTC408.3164196E-944.00000432
GACGTAA207.857327E-444.00000426
GATCCGC207.857327E-444.00000410
TAAGTGC207.857327E-444.00000432
CCTAAGT650.044.00000420
TACATAG650.044.0000041
ATCCGCC207.857327E-444.00000411
CGTAGTG207.857327E-444.00000438
TACGATG650.044.0000041
ATATACG207.857327E-444.0000041
CAAGGCA207.857327E-444.00000444
ACGGATT207.857327E-444.00000440
ACTACGG207.857327E-444.0000042
GAACGAT207.857327E-444.0000049
Produced by FastQC (version 0.11.5)