##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546259_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1547771
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91879160418434	33.0	31.0	34.0	30.0	34.0
2	32.27839648113319	33.0	31.0	34.0	30.0	34.0
3	32.1291159997183	33.0	31.0	34.0	30.0	34.0
4	35.90855236336642	37.0	35.0	37.0	35.0	37.0
5	35.90093624961315	37.0	35.0	37.0	35.0	37.0
6	35.96687236031687	37.0	35.0	37.0	35.0	37.0
7	36.2052435405496	37.0	35.0	37.0	35.0	37.0
8	36.101452346632676	37.0	35.0	37.0	35.0	37.0
9	37.90914030563953	39.0	38.0	39.0	35.0	39.0
10	37.56960299682576	39.0	37.0	39.0	35.0	39.0
11	37.33156778360623	39.0	37.0	39.0	34.0	39.0
12	36.471158847142114	38.0	35.0	39.0	33.0	39.0
13	36.15305364940938	39.0	35.0	39.0	33.0	39.0
14	37.25887292112334	40.0	35.0	41.0	33.0	41.0
15	37.519583969463184	40.0	35.0	41.0	33.0	41.0
16	37.66575804818672	40.0	35.0	41.0	34.0	41.0
17	37.59356842840446	39.0	35.0	41.0	34.0	41.0
18	37.40819539841488	39.0	35.0	41.0	34.0	41.0
19	37.09517040957609	37.0	35.0	41.0	34.0	41.0
20	36.75523510906975	36.0	35.0	41.0	33.0	41.0
21	36.628235701534656	36.0	35.0	40.0	33.0	41.0
22	36.54069691188167	36.0	35.0	40.0	33.0	41.0
23	36.52344048312057	35.0	35.0	40.0	33.0	41.0
24	36.438202421417635	35.0	35.0	40.0	33.0	41.0
25	36.42080062231428	35.0	35.0	40.0	33.0	41.0
26	36.36715508948029	35.0	35.0	40.0	33.0	41.0
27	36.2842739655931	35.0	35.0	40.0	32.0	41.0
28	36.197850327987794	36.0	35.0	40.0	32.0	41.0
29	36.08840325862159	36.0	35.0	40.0	32.0	41.0
30	35.849877016690456	36.0	35.0	40.0	31.0	41.0
31	35.44473116501084	36.0	35.0	40.0	30.0	41.0
32	34.98222476063965	35.0	35.0	40.0	27.0	41.0
33	34.47117112285991	35.0	34.0	40.0	23.0	41.0
34	33.94128394962821	35.0	34.0	40.0	18.0	41.0
35	33.670747804423264	35.0	34.0	40.0	17.0	41.0
36	33.45279695768948	35.0	33.0	40.0	15.0	41.0
37	33.42627365417752	35.0	33.0	40.0	15.0	41.0
38	33.398655873511004	35.0	33.0	40.0	15.0	41.0
39	33.352625808339866	35.0	33.0	40.0	15.0	41.0
40	33.165852700431785	35.0	33.0	40.0	15.0	41.0
41	33.04431921776542	35.0	33.0	40.0	12.0	41.0
42	32.94823975898243	35.0	33.0	40.0	10.0	41.0
43	32.79466536070259	35.0	33.0	40.0	10.0	41.0
44	32.65638198415657	35.0	33.0	40.0	10.0	41.0
45	32.5913581531118	35.0	33.0	40.0	10.0	41.0
46	32.526466770601075	35.0	33.0	40.0	10.0	41.0
47	32.533014896906586	35.0	32.0	40.0	10.0	41.0
48	32.493623410698355	35.0	32.0	40.0	10.0	41.0
49	32.41488824897223	35.0	32.0	40.0	10.0	41.0
50	32.22879353599467	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	6.0
13	14.0
14	9.0
15	28.0
16	125.0
17	266.0
18	689.0
19	1377.0
20	2638.0
21	4423.0
22	6947.0
23	11058.0
24	17163.0
25	28691.0
26	44747.0
27	53201.0
28	48924.0
29	41032.0
30	37570.0
31	39275.0
32	46480.0
33	59577.0
34	118799.0
35	275377.0
36	72528.0
37	88371.0
38	153623.0
39	394829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.735608820684714	23.269010725746895	27.82549873333975	22.169881720228638
2	37.85876592855145	22.703810835065394	29.21504537815995	10.222377858223213
3	22.333084157798538	23.28761812955534	43.19256530843387	11.18673240421225
4	21.965071060253745	26.216604394319315	40.870322547715396	10.948001997711549
5	18.104810078493525	30.119571952181555	40.22933625193908	11.54628171738584
6	16.298535119213373	38.23640577320547	37.11421134004966	8.350847767531503
7	77.64630555812198	3.398823210927198	17.72135542014936	1.2335158108014685
8	77.25309493458657	3.0760364420834865	17.512086736345363	2.158781886984573
9	75.29201671306673	3.773814084900157	18.761754807397217	2.172414394635899
10	44.007285315463335	23.333038285379427	23.97945174059987	8.68022465855737
11	38.28880370545772	17.44037070083365	32.86287183310709	11.407953760601536
12	37.48099686581542	18.241393591170787	34.066150612719845	10.21145893029395
13	14.931149375456707	40.72101105396083	31.81168273601198	12.53615683457049
14	9.513358242272274	41.328400648416334	35.79709143019219	13.361149679119197
15	8.417072034558084	22.441304301476123	58.09625584146492	11.045367822500873
16	9.774443376959512	17.81620149233963	54.88363588670418	17.52571924399669
17	9.92931124824021	20.03003028225752	36.42838636981827	33.612272099684
18	12.676035408338832	23.624425060296385	47.062259210180315	16.637280321184463
19	18.119928594087888	23.277539119159098	38.215343225838964	20.38718906091405
20	21.91047642060744	20.081006815607736	41.30481834845077	16.70369841533405
21	14.782936235399163	27.501355174635005	40.65039337214614	17.06531521781969
22	19.394018882638324	19.756798647861988	34.83958544254932	26.00959702695037
23	12.22726100954211	26.15244761660478	34.977138090841606	26.643153283011507
24	11.382885452692937	22.40383105769523	52.96881773854143	13.244465751070411
25	11.232798650446352	25.9857562908208	46.11366927019566	16.66777578853719
26	10.873701600559773	30.7114553767967	37.21551831634008	21.19932470630345
27	11.753289084754787	36.90591179186068	34.38887277252255	16.95192635086198
28	10.028356908095578	30.497470232999586	43.1771237476345	16.297049111270336
29	9.76391210327626	22.6829421148219	41.848632646560766	25.704513135341077
30	12.318682802559293	32.45693322849439	38.685180172002184	16.539203796944122
31	19.2506514206559	31.49115728360332	34.04586337384535	15.212327921895422
32	20.665266373384693	24.925780364149478	37.03261012126471	17.37634314120112
33	21.147508255420213	31.073718269692353	31.111708385801258	16.667065089086176
34	15.3537571126478	26.73425203082368	34.70571550959412	23.206275346934397
35	16.554645357743492	27.514406200917318	33.15135120117898	22.779597240160204
36	25.584081882914205	27.82136375471565	31.615594296572297	14.978960065797848
37	14.91964896615843	31.395858948126047	38.13374200705402	15.550750078661506
38	14.191052810784024	30.220039010938958	35.11242942269884	20.476478755578185
39	16.34737955421054	30.04372093804574	36.232427148460594	17.376472359283124
40	18.673175812184102	26.340976798247283	32.99764629263631	21.988201096932297
41	13.116087586600344	27.59200165916017	35.85633792079061	23.435572833448877
42	17.207907371310096	26.01379661461547	32.90357552893807	23.874720485136365
43	16.770891817975656	24.39979816135591	34.8428158945994	23.98649412606904
44	14.599704995118786	26.758351203117257	34.249963334369234	24.391980467394724
45	13.862515837291175	36.70820812639596	29.738443219313453	19.690832816999414
46	20.80333589400499	30.93099689811994	32.24411104743531	16.021556160439754
47	15.923544245240414	30.539595327732588	33.74930787564827	19.787552551378727
48	16.540108323518144	28.115076455108667	35.26529441370849	20.0795208076647
49	20.050446739214006	24.741063115926064	35.77195851324259	19.43653163161734
50	18.448465567580737	29.421794309364884	31.95188435498533	20.17785576806905
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3529.0
1	4670.5
2	5812.0
3	22079.5
4	38347.0
5	28895.0
6	19443.0
7	21468.0
8	23493.0
9	26157.5
10	28822.0
11	28770.5
12	28719.0
13	26883.5
14	25048.0
15	22321.5
16	19595.0
17	18449.5
18	17304.0
19	16589.5
20	15875.0
21	14363.5
22	12852.0
23	13504.5
24	14157.0
25	16214.0
26	18271.0
27	20322.0
28	22373.0
29	31381.5
30	40390.0
31	47737.0
32	55084.0
33	69323.0
34	83562.0
35	84619.5
36	85677.0
37	83115.5
38	80554.0
39	90232.5
40	99911.0
41	115409.5
42	130908.0
43	137594.5
44	144281.0
45	136724.0
46	129167.0
47	118996.0
48	108825.0
49	99070.5
50	89316.0
51	74185.0
52	59054.0
53	49325.5
54	39597.0
55	33957.0
56	28317.0
57	23347.0
58	18377.0
59	16703.5
60	15030.0
61	14809.0
62	14588.0
63	12562.5
64	10537.0
65	8697.0
66	6857.0
67	6092.0
68	5327.0
69	4438.5
70	3550.0
71	2860.0
72	2170.0
73	1852.5
74	1535.0
75	1063.5
76	592.0
77	474.0
78	356.0
79	325.5
80	295.0
81	229.5
82	164.0
83	97.0
84	30.0
85	40.0
86	50.0
87	31.5
88	13.0
89	8.5
90	4.0
91	8.0
92	12.0
93	6.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1547771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.401961979589636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.39455634350165	16.991992762500093
2	8.659920189436452	3.706785652811992
3	3.4895623966575156	2.2405044521600948
4	1.811508925406648	1.5507938058896142
5	1.0960552349429311	1.17288662328894
6	0.7639690462395923	0.9810261888721866
7	0.5071710944874301	0.7598119536956789
8	0.38337771762177286	0.6564028269090426
9	0.3000738574222721	0.577995235885824
>10	2.211897555125968	10.214566447025659
>50	0.6911057915032242	10.833202364604231
>100	0.5668585082337515	23.95865094323125
>500	0.08232511025257054	12.468144183650658
>1k	0.041314446473614735	12.362038958134326
>5k	0.0	0.0
>10k+	3.037826946589319E-4	1.52519760134045
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23459	1.515663492855209	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	4506	0.2911283387529551	No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	3017	0.19492547670165677	No Hit
GTAAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	2780	0.17961313398429096	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2636	0.17030943208006868	No Hit
ACTTAGGGGATTTTTGAAAGATTCAACTTGTACTGCTGGTATTGTGTAGT	2279	0.14724400444251767	No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC	2150	0.13890943815331855	No Hit
TGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	2117	0.1367773398002676	No Hit
GAATTAGGGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGC	1895	0.12243413269792496	No Hit
CTCACTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1865	0.12049586146787865	No Hit
GATAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1839	0.11881602640183853	No Hit
TTAGCTGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC	1819	0.11752384558180766	No Hit
TTAAATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1801	0.11636088284377986	No Hit
GTCGACGGGGGTTTTTAATATTCGATAAATCCATAAATAAAAATATATCA	1736	0.11216129517867954	No Hit
AGCTATGGGACAACCTCAATCTTATTAATCTTCATTCTTCTACTATCCCC	1733	0.1119674680556749	No Hit
ATAGATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	1722	0.11125676860465793	No Hit
TAGTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1722	0.11125676860465793	No Hit
TAAGTTGGGATGATATTAAAATCCCAACTATACCAAAGATATCCCAATTA	1662	0.10738022614456531	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGC	1651	0.10666952669354833	No Hit
AAGCACGGGATCAACAATAATTTTAAATAATCTAAGTATAGGTAATATAA	1645	0.10628187244753907	No Hit
TTTCTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1642	0.10608804532453445	No Hit
GACATCGGGATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATT	1633	0.10550656395552055	No Hit
AATACGGGGAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCAT	1612	0.10414977409448814	No Hit
GGAACTGGGAATCTAAGTATAGGTAATATAACAATTAAAAAGATTTAGAG	1609	0.10395594697148351	No Hit
TTACGAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	1591	0.10279298423345573	No Hit
GAGATTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1586	0.102469939028448	No Hit
TGGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT	1581	0.10214689382344029	No Hit
TGGAGAGTTGTATTTTGTTTAAAGAACATTTTCTGATTTTCGAATGTATT	1551	0.10020862259339398	No Hit
GTTCTAGGGAAGTCTAGTGGAACAGTCAGTTTAACTTTTTAACAGATCTT	1549	0.10007940451139088	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09025883027915628	0.0
2	0.0	0.0	0.0	0.36374890083868994	0.0
3	0.0	0.0	0.0	0.5332184153857386	0.0
4	0.0	0.0	0.0	0.9328253339802852	0.0
5	0.0	0.0	0.0	1.5779466083806972	0.0
6	0.0	0.0	0.0	2.212924263343867	0.0
7	0.0	0.0	0.0	2.554964526406038	0.0
8	6.460904100154351E-5	0.0	0.0	3.3921038706630373	0.0
9	6.460904100154351E-5	0.0	0.0	3.7067499003405544	0.0
10	6.460904100154351E-5	0.0	0.0	4.260320163641779	0.0
11	6.460904100154351E-5	0.0	0.0	4.884960372044702	0.0
12	6.460904100154351E-5	0.0	0.0	5.417597306061427	0.0
13	6.460904100154351E-5	0.0	0.0	5.599342538398768	0.0
14	6.460904100154351E-5	0.0	0.0	5.6601396459812205	0.0
15	6.460904100154351E-5	0.0	0.0	5.761963494599653	0.0
16	6.460904100154351E-5	0.0	0.0	5.993328470426181	0.0
17	6.460904100154351E-5	0.0	0.0	6.268369157969751	0.0
18	6.460904100154351E-5	0.0	0.0	6.792865352820281	0.0
19	6.460904100154351E-5	0.0	0.0	6.976031984059658	0.0
20	6.460904100154351E-5	0.0	0.0	7.173864867606383	0.0
21	6.460904100154351E-5	0.0	0.0	7.474038472099555	0.0
22	6.460904100154351E-5	0.0	0.0	7.735963524319812	0.0
23	1.2921808200308703E-4	0.0	0.0	8.090279505172276	0.0
24	1.2921808200308703E-4	0.0	0.0	8.330625137698018	0.0
25	1.2921808200308703E-4	0.0	0.0	8.514373250306408	0.0
26	1.2921808200308703E-4	0.0	0.0	8.737080614638728	0.0
27	1.2921808200308703E-4	0.0	0.0	8.90835918233382	0.0
28	1.2921808200308703E-4	0.0	0.0	9.095143919869283	0.0
29	1.2921808200308703E-4	0.0	0.0	9.316559103381573	0.0
30	1.2921808200308703E-4	0.0	0.0	9.684701419008368	0.0
31	1.2921808200308703E-4	0.0	0.0	10.047028920945024	0.0
32	1.9382712300463053E-4	0.0	0.0	10.299844098384064	0.0
33	1.9382712300463053E-4	0.0	0.0	10.51654282190324	0.0
34	1.9382712300463053E-4	0.0	0.0	10.756306973059969	0.0
35	1.9382712300463053E-4	0.0	0.0	11.143702782905223	0.0
36	1.9382712300463053E-4	0.0	0.0	11.400394502804355	0.0
37	1.9382712300463053E-4	0.0	0.0	11.661091983245583	0.0
38	1.9382712300463053E-4	0.0	0.0	11.90751086562547	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	80	0.0	44.000004	19
AGCCGAA	20	7.857327E-4	44.000004	37
TCCGCGT	20	7.857327E-4	44.000004	23
CGTATGG	55	1.8189894E-12	44.000004	2
GGTCGTA	20	7.857327E-4	44.000004	8
ACGTTGG	65	0.0	44.000004	2
TAGCGTC	40	8.3164196E-9	44.000004	32
GACGTAA	20	7.857327E-4	44.000004	26
GATCCGC	20	7.857327E-4	44.000004	10
TAAGTGC	20	7.857327E-4	44.000004	32
CCTAAGT	65	0.0	44.000004	20
TACATAG	65	0.0	44.000004	1
ATCCGCC	20	7.857327E-4	44.000004	11
CGTAGTG	20	7.857327E-4	44.000004	38
TACGATG	65	0.0	44.000004	1
ATATACG	20	7.857327E-4	44.000004	1
CAAGGCA	20	7.857327E-4	44.000004	44
ACGGATT	20	7.857327E-4	44.000004	40
ACTACGG	20	7.857327E-4	44.000004	2
GAACGAT	20	7.857327E-4	44.000004	9
>>END_MODULE