FastQCFastQC Report
Thu 26 May 2016
SRR1546258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546258_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1586086
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242351.5279751539323845No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC47040.2965791262264467No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA30370.19147763740427695No Hit
GTAAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT28890.1821464914260639No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26980.17010426925147817No Hit
ACTTAGGGGATTTTTGAAAGATTCAACTTGTACTGCTGGTATTGTGTAGT23760.14980272192050118No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC21780.13731916176045939No Hit
TGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA20980.13227529906953342No Hit
GAATTAGGGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGC19160.12080051144767687No Hit
CTCACTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT18860.11890906293857961No Hit
GATAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT18520.11676542129493608No Hit
GTCGACGGGGGTTTTTAATATTCGATAAATCCATAAATAAAAATATATCA18120.1142434899494731No Hit
TTAGCTGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC17960.11323471741128792No Hit
GAGATTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC17680.11146936546946383No Hit
ATAGATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG17590.11090193091673466No Hit
AGCTATGGGACAACCTCAATCTTATTAATCTTCATTCTTCTACTATCCCC17570.1107758343494615No Hit
TTAAATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT17440.10995620666218603No Hit
TTTCTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT17360.10945182039309345No Hit
TTACGAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA17270.10888438584036426No Hit
AATACGGGGAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCAT16680.10516453710580638No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGC16580.10453405426944062No Hit
AAGCACGGGATCAACAATAATTTTAAATAATCTAAGTATAGGTAATATAA16530.10421881285125774No Hit
TAAGTTGGGATGATATTAAAATCCCAACTATACCAAAGATATCCCAATTA16470.1038405231494383No Hit
GACATCGGGATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATT16300.10276870232761653No Hit
GGAACTGGGAATCTAAGTATAGGTAATATAACAATTAAAAAGATTTAGAG16270.1025795574767068No Hit
TAGTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT16240.10239041262579708No Hit
ATAGTAGGGCACCATCATGCTATTAATGATATTAAAATCCCAACTATACC16210.10220126777488736No Hit
AGAGTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA15940.10049896411669985No Hit
GGTGTTGGGCATTTTGTAATTTTCATTTAAATTGTTAGGAGGCTTGGAGC15890.10018372269851698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCG302.528328E-644.0000041
GGTACTC207.8573864E-444.0000048
GCCCATA207.8573864E-444.00000411
ACACGTC207.8573864E-444.00000427
CGAACGC207.8573864E-444.00000412
TCCGCGT207.8573864E-444.00000423
TTGTGCG600.044.0000041
GCGAACG207.8573864E-444.00000411
ACCGACA207.8573864E-444.00000418
ATTAACG408.3164196E-944.0000041
TTCGTGC302.528328E-644.00000439
CTAAGCG408.3164196E-944.0000041
TACGCGG207.8573864E-444.0000042
TACGATG408.3164196E-944.0000041
CGGATGT207.8573864E-444.00000437
CTGCCTA207.8573864E-444.00000425
ACGGACT302.528328E-644.00000444
ACTACGG408.3164196E-944.0000042
TGTTACG408.3164196E-944.0000041
ACGTCGA207.8573864E-444.00000429
Produced by FastQC (version 0.11.5)