##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546258_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1586086
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85424434740613	31.0	31.0	34.0	30.0	34.0
2	32.25082435630855	33.0	31.0	34.0	30.0	34.0
3	32.2281584983412	33.0	31.0	34.0	30.0	34.0
4	35.943992318197125	37.0	35.0	37.0	35.0	37.0
5	35.8573368657185	37.0	35.0	37.0	35.0	37.0
6	35.9530454212445	37.0	35.0	37.0	35.0	37.0
7	36.182553783338356	37.0	35.0	37.0	35.0	37.0
8	36.08158384854289	37.0	35.0	37.0	35.0	37.0
9	37.88220500023328	39.0	38.0	39.0	35.0	39.0
10	37.565322435227344	39.0	37.0	39.0	35.0	39.0
11	37.32893424442307	39.0	37.0	39.0	34.0	39.0
12	36.45890323727717	38.0	35.0	39.0	33.0	39.0
13	36.08266449612442	38.0	35.0	39.0	32.0	39.0
14	37.187325277443975	39.0	35.0	41.0	33.0	41.0
15	37.489708628662	40.0	35.0	41.0	33.0	41.0
16	37.630103285698254	40.0	35.0	41.0	33.0	41.0
17	37.56404003313818	39.0	35.0	41.0	33.0	41.0
18	37.39053304801883	39.0	35.0	41.0	33.0	41.0
19	37.08330506668617	37.0	35.0	41.0	33.0	41.0
20	36.741212014985315	36.0	35.0	40.0	33.0	41.0
21	36.61102424458699	36.0	35.0	40.0	33.0	41.0
22	36.53062065991377	36.0	35.0	40.0	33.0	41.0
23	36.50514473994475	35.0	35.0	40.0	33.0	41.0
24	36.42262021101	35.0	35.0	40.0	33.0	41.0
25	36.39836931919202	35.0	35.0	40.0	33.0	41.0
26	36.36220104080107	35.0	35.0	40.0	33.0	41.0
27	36.269436209638066	35.0	35.0	40.0	32.0	41.0
28	36.17316400245636	36.0	35.0	40.0	32.0	41.0
29	36.065736031968	36.0	35.0	40.0	32.0	41.0
30	35.82683978044066	36.0	35.0	40.0	31.0	41.0
31	35.429607852285436	36.0	35.0	40.0	30.0	41.0
32	34.969444910301206	35.0	35.0	40.0	27.0	41.0
33	34.447840154947464	35.0	34.0	40.0	23.0	41.0
34	33.995744240854535	35.0	34.0	40.0	18.0	41.0
35	33.692149101625006	35.0	34.0	40.0	17.0	41.0
36	33.45524895875759	35.0	33.0	40.0	15.0	41.0
37	33.36837094583774	35.0	33.0	40.0	15.0	41.0
38	33.34847542945339	35.0	33.0	40.0	15.0	41.0
39	33.308681874753326	35.0	33.0	40.0	15.0	41.0
40	33.125436451743475	35.0	33.0	40.0	15.0	41.0
41	33.02781501129195	35.0	33.0	40.0	12.0	41.0
42	32.92014052201457	35.0	33.0	40.0	10.0	41.0
43	32.800378415798384	35.0	33.0	40.0	10.0	41.0
44	32.659301576333185	35.0	33.0	40.0	10.0	41.0
45	32.59336378985755	35.0	33.0	40.0	10.0	41.0
46	32.538591854413944	35.0	33.0	40.0	10.0	41.0
47	32.518655987128064	35.0	32.0	40.0	10.0	41.0
48	32.49229108635976	35.0	32.0	40.0	10.0	41.0
49	32.40392261201473	35.0	32.0	40.0	10.0	41.0
50	32.20971372296332	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	2.0
12	2.0
13	6.0
14	19.0
15	43.0
16	115.0
17	302.0
18	687.0
19	1502.0
20	2708.0
21	4537.0
22	7229.0
23	11141.0
24	17712.0
25	29413.0
26	45010.0
27	54062.0
28	50698.0
29	42154.0
30	39685.0
31	40710.0
32	48365.0
33	62369.0
34	124792.0
35	277514.0
36	75488.0
37	91202.0
38	158652.0
39	399962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.903648351980912	23.30485232200524	27.77024701056563	22.021252315448216
2	37.90796968134136	22.67109097489039	29.2071804429268	10.213758900841443
3	22.167398236917794	23.345266271816282	43.26240821746109	11.224927273804825
4	22.049434898233766	26.229536103338656	40.76771373052912	10.95331526789846
5	18.206642010584545	29.931226932209228	40.247754535378284	11.614376521827946
6	16.271501040927163	38.242440826033395	37.11217424528052	8.373883887758923
7	77.60720414908145	3.4104077584695913	17.72325081994293	1.2591372725060306
8	77.18932012513824	3.107082465894031	17.518406946407698	2.185190462560038
9	75.24932443764084	3.7666305610162376	18.785866592353756	2.198178408989172
10	44.05542952904193	23.268473462346932	23.958095588763786	8.718001419847347
11	38.274091064418954	17.482973811003944	32.84689480898261	11.396040315594488
12	37.494940375238166	18.187412284075393	34.026717340673834	10.29093000001261
13	14.938218986864522	40.651452695503274	31.868574591793887	12.541753725838323
14	9.504339613362706	41.295553961134516	35.85934180113814	13.340764624364631
15	8.461079664028306	22.34090711348565	58.12515840881264	11.072854813673407
16	9.834523474767447	17.77602223334674	54.9251427728383	17.464311519047516
17	9.929032851938672	20.007237942961478	36.404205068325425	33.65952413677443
18	12.668480775947835	23.59708111666076	47.09082609644118	16.643612010950225
19	18.15500546628619	23.259457557786906	38.275856416360774	20.309680559566125
20	21.887337761004133	20.064927122488946	41.29794979591271	16.749785320594217
21	14.767862524478495	27.393596564120738	40.80119236914013	17.037348542260634
22	19.35178798627565	19.70498447120774	34.83121343987652	26.112014102640085
23	12.217559451379055	26.094297534938203	35.02792408482264	26.660218928860104
24	11.356572090037993	22.381888497849424	52.96944806271539	13.292091349397195
25	11.266602189288601	25.84134782098827	46.15254153936167	16.739508450361456
26	10.834658398094428	30.6270908387061	37.266768636757405	21.27148212644207
27	11.75970281561025	36.87568013335973	34.37234803156954	16.992269019460483
28	10.007969303051663	30.492987139411103	43.11664058569333	16.3824029718439
29	9.80501687802553	22.688744494308633	41.85037885713637	25.655859770529467
30	12.344475646339479	32.431911006086686	38.615308375460096	16.608304972113743
31	19.25299132581714	31.444007449785193	34.05748490308848	15.245516321309186
32	20.72031403089114	24.83894315945037	37.11166985901143	17.329072950647063
33	21.114554948470637	31.064015444307557	31.101970511056777	16.719459096165025
34	15.379304779185995	26.72553695070759	34.539047693504635	23.356110576601775
35	16.507175525160676	27.464841124630063	33.077021044256114	22.950962305953144
36	25.752575837627973	27.768418610340174	31.43083035850515	15.048175193526706
37	14.986955310115594	31.456491009945236	38.02637435801085	15.530179321928319
38	14.203012951378424	30.164379485097275	35.093368203237404	20.539239360286896
39	16.348546043531055	30.05114476768599	36.23914466176487	17.36116452701808
40	18.786686220041034	26.18552840136033	32.97690036984123	22.050885008757408
41	13.155717911891285	27.530474387895737	35.847677868665386	23.466129831547597
42	17.28588487635601	25.97816259647964	32.92526382554288	23.810688701621476
43	16.772608799270657	24.27113031701938	34.88694812261126	24.069312761098704
44	14.574115148863303	26.64092615406731	34.301040422776566	24.48391827429282
45	13.909397094482898	36.560312618609586	29.736722977190393	19.793567309717126
46	20.840925397487904	30.882940773703314	32.14750019860209	16.128633630206686
47	15.937219041086045	30.468650501927385	33.68278895343632	19.911341503550247
48	16.557740248637213	28.18113267502519	35.28415230952168	19.97697476681592
49	20.13182135142735	24.737624567646396	35.682932703523015	19.447621377403244
50	18.48708077620003	29.300996288978027	31.911699617801304	20.30022331702064
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3661.0
1	4830.0
2	5999.0
3	22789.5
4	39580.0
5	29672.0
6	19764.0
7	21750.5
8	23737.0
9	26362.0
10	28987.0
11	29177.5
12	29368.0
13	27497.5
14	25627.0
15	22801.0
16	19975.0
17	18698.5
18	17422.0
19	16722.0
20	16022.0
21	14665.0
22	13308.0
23	13911.0
24	14514.0
25	16605.0
26	18696.0
27	20785.5
28	22875.0
29	32086.5
30	41298.0
31	48701.0
32	56104.0
33	70474.0
34	84844.0
35	86279.0
36	87714.0
37	84664.5
38	81615.0
39	92110.0
40	102605.0
41	117898.0
42	133191.0
43	140693.5
44	148196.0
45	140607.5
46	133019.0
47	122945.5
48	112872.0
49	102614.5
50	92357.0
51	76694.5
52	61032.0
53	51060.5
54	41089.0
55	35033.5
56	28978.0
57	23955.5
58	18933.0
59	17299.0
60	15665.0
61	15618.5
62	15572.0
63	13101.0
64	10630.0
65	8761.0
66	6892.0
67	6036.0
68	5180.0
69	4397.0
70	3614.0
71	2881.0
72	2148.0
73	1875.5
74	1603.0
75	1053.5
76	504.0
77	426.5
78	349.0
79	316.0
80	283.0
81	226.5
82	170.0
83	91.0
84	12.0
85	33.5
86	55.0
87	32.0
88	9.0
89	8.5
90	8.0
91	9.0
92	10.0
93	5.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1586086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.48515209265839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.42293096479582	17.064137514233458
2	8.649511823082388	3.716721540923441
3	3.469153835278	2.2360589335133096
4	1.7981024067602764	1.5453001474967853
5	1.119338338960806	1.2024577227858264
6	0.762274825673934	0.9826554339605479
7	0.5372716725138799	0.8080354519326362
8	0.37456512301804895	0.6438070909318465
9	0.28780530581940256	0.5565186691743532
>10	2.219082976908196	10.264155691546211
>50	0.6721554391020229	10.55405832527385
>100	0.5608183941940188	23.4990557445351
>500	0.08564367262563934	12.94239910826595
>1k	0.041049898258496094	12.446913391140166
>5k	0.0	0.0
>10k+	2.953230090539288E-4	1.5377252342865106
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24235	1.5279751539323845	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	4704	0.2965791262264467	No Hit
AGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	3037	0.19147763740427695	No Hit
GTAAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	2889	0.1821464914260639	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2698	0.17010426925147817	No Hit
ACTTAGGGGATTTTTGAAAGATTCAACTTGTACTGCTGGTATTGTGTAGT	2376	0.14980272192050118	No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC	2178	0.13731916176045939	No Hit
TGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	2098	0.13227529906953342	No Hit
GAATTAGGGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGC	1916	0.12080051144767687	No Hit
CTCACTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1886	0.11890906293857961	No Hit
GATAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1852	0.11676542129493608	No Hit
GTCGACGGGGGTTTTTAATATTCGATAAATCCATAAATAAAAATATATCA	1812	0.1142434899494731	No Hit
TTAGCTGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC	1796	0.11323471741128792	No Hit
GAGATTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1768	0.11146936546946383	No Hit
ATAGATGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	1759	0.11090193091673466	No Hit
AGCTATGGGACAACCTCAATCTTATTAATCTTCATTCTTCTACTATCCCC	1757	0.1107758343494615	No Hit
TTAAATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1744	0.10995620666218603	No Hit
TTTCTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1736	0.10945182039309345	No Hit
TTACGAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	1727	0.10888438584036426	No Hit
AATACGGGGAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCAT	1668	0.10516453710580638	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGC	1658	0.10453405426944062	No Hit
AAGCACGGGATCAACAATAATTTTAAATAATCTAAGTATAGGTAATATAA	1653	0.10421881285125774	No Hit
TAAGTTGGGATGATATTAAAATCCCAACTATACCAAAGATATCCCAATTA	1647	0.1038405231494383	No Hit
GACATCGGGATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATT	1630	0.10276870232761653	No Hit
GGAACTGGGAATCTAAGTATAGGTAATATAACAATTAAAAAGATTTAGAG	1627	0.1025795574767068	No Hit
TAGTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1624	0.10239041262579708	No Hit
ATAGTAGGGCACCATCATGCTATTAATGATATTAAAATCCCAACTATACC	1621	0.10220126777488736	No Hit
AGAGTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA	1594	0.10049896411669985	No Hit
GGTGTTGGGCATTTTGTAATTTTCATTTAAATTGTTAGGAGGCTTGGAGC	1589	0.10018372269851698	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08959161104757245	0.0
2	0.0	0.0	0.0	0.361455810088482	0.0
3	0.0	0.0	0.0	0.5430979152454533	0.0
4	0.0	0.0	0.0	0.9498223929849958	0.0
5	0.0	0.0	0.0	1.6042635771326397	0.0
6	0.0	0.0	0.0	2.255300153963909	0.0
7	0.0	0.0	0.0	2.6046506935941682	0.0
8	0.0	0.0	0.0	3.457315681495203	0.0
9	0.0	0.0	0.0	3.76934163721261	0.0
10	0.0	0.0	0.0	4.335767417403596	0.0
11	0.0	0.0	0.0	4.984975594009405	0.0
12	0.0	0.0	0.0	5.529082281793043	0.0
13	0.0	0.0	0.0	5.724721105917333	0.0
14	0.0	0.0	0.0	5.787328051568452	0.0
15	0.0	0.0	0.0	5.892366492106985	0.0
16	0.0	0.0	0.0	6.142731226427823	0.0
17	0.0	0.0	0.0	6.436410131606987	0.0
18	0.0	0.0	0.0	6.976292584386975	0.0
19	0.0	0.0	0.0	7.175399064111278	0.0
20	0.0	0.0	0.0	7.3813147584683305	0.0
21	0.0	0.0	0.0	7.6893686723166335	0.0
22	0.0	0.0	0.0	7.963943947553916	0.0
23	0.0	0.0	0.0	8.337063690115164	0.0
24	0.0	0.0	0.0	8.59039169376692	0.0
25	0.0	0.0	0.0	8.778653868705732	0.0
26	0.0	0.0	0.0	9.015904560030162	0.0
27	0.0	0.0	0.0	9.196537892648948	0.0
28	0.0	0.0	0.0	9.394446454984156	0.0
29	0.0	0.0	0.0	9.62759900787221	0.0
30	0.0	0.0	0.0	9.99863815707345	0.0
31	6.304828363657456E-5	0.0	0.0	10.373964589561979	0.0
32	6.304828363657456E-5	0.0	0.0	10.644000388377426	0.0
33	6.304828363657456E-5	0.0	0.0	10.869839340363637	0.0
34	6.304828363657456E-5	0.0	0.0	11.120582364386294	0.0
35	6.304828363657456E-5	0.0	0.0	11.516651682191256	0.0
36	6.304828363657456E-5	0.0	0.0	11.786750529290341	0.0
37	6.304828363657456E-5	0.0	0.0	12.046572506156664	0.0
38	6.304828363657456E-5	0.0	0.0	12.29605456450659	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	30	2.528328E-6	44.000004	1
GGTACTC	20	7.8573864E-4	44.000004	8
GCCCATA	20	7.8573864E-4	44.000004	11
ACACGTC	20	7.8573864E-4	44.000004	27
CGAACGC	20	7.8573864E-4	44.000004	12
TCCGCGT	20	7.8573864E-4	44.000004	23
TTGTGCG	60	0.0	44.000004	1
GCGAACG	20	7.8573864E-4	44.000004	11
ACCGACA	20	7.8573864E-4	44.000004	18
ATTAACG	40	8.3164196E-9	44.000004	1
TTCGTGC	30	2.528328E-6	44.000004	39
CTAAGCG	40	8.3164196E-9	44.000004	1
TACGCGG	20	7.8573864E-4	44.000004	2
TACGATG	40	8.3164196E-9	44.000004	1
CGGATGT	20	7.8573864E-4	44.000004	37
CTGCCTA	20	7.8573864E-4	44.000004	25
ACGGACT	30	2.528328E-6	44.000004	44
ACTACGG	40	8.3164196E-9	44.000004	2
TGTTACG	40	8.3164196E-9	44.000004	1
ACGTCGA	20	7.8573864E-4	44.000004	29
>>END_MODULE