FastQCFastQC Report
Thu 26 May 2016
SRR1546257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546257_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1761570
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293611.6667518179805514No Hit
ATGTAGGGGGTATTACTAGCGTTTCTGTGGCATGATTATGCATTCTGTAA23870.13550412416196914No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21520.12216375165335469No Hit
AATGCAGGGATTTCGTGTTTGTTTTAACCTTGACTCTCTTTTGATTCTTT18990.10780156337812292No Hit
GGTGGGTGGATATGGGGGACTTTTGGTATAGCATTGGAAATATAAATGAG18610.10564439675970867No Hit
AGACGAGGGAAATTCCTAAACCAACAACATTTTTTGAAGATGCAGTATAG17810.10110299335252075No Hit
GGTTTGAGGAGATGAGGTAATTTTTATTATTTTAGGAGTTTCAAGCATTG17740.10070562055439182No Hit
TATGAAGGGACCAAACACAAATACGAAAAATTATAGCCTATTCATCAATT17710.10053531792662228No Hit
GTAAGAGGGCTTACTTTATTGTTGTAACCATTTCATAATGTAAATGTTTA17660.10025148021367303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA750.044.00000431
CGGCGTA254.4438093E-544.021
CCTTACG302.5284899E-644.028
ACGCCTA254.4438093E-544.010
ACGTTAG207.857626E-444.01
ATCGTAG408.3164196E-944.01
ACGATTC207.857626E-444.030
AACGCAC302.5284899E-644.032
TGTTACG302.5284899E-644.01
CGTACTC207.857626E-444.044
ACGTCGT207.857626E-444.015
ACCGTAA254.4438093E-544.035
ACGTAAG207.857626E-444.01
ACATCGC207.857626E-444.09
ATACGAT551.8189894E-1244.044
GCCACTA207.857626E-444.010
CGTTTCG351.4466787E-744.042
CCGTTTA254.4438093E-544.028
CCGTTCA207.857626E-444.022
ATCGCAA302.5284899E-644.014
Produced by FastQC (version 0.11.5)