##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546257_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1761570
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.926269180333453	33.0	31.0	34.0	30.0	34.0
2	32.2763710780724	33.0	31.0	34.0	30.0	34.0
3	32.155374467094695	33.0	31.0	34.0	30.0	34.0
4	35.91777959433914	37.0	35.0	37.0	35.0	37.0
5	35.93026107392837	37.0	35.0	37.0	35.0	37.0
6	35.98057584995203	37.0	35.0	37.0	35.0	37.0
7	36.20993204925152	37.0	35.0	37.0	35.0	37.0
8	36.119045510538896	37.0	36.0	37.0	35.0	37.0
9	37.876534000919634	39.0	38.0	39.0	35.0	39.0
10	37.47784987255687	39.0	37.0	39.0	35.0	39.0
11	37.31929869377885	39.0	37.0	39.0	34.0	39.0
12	36.79570667075393	39.0	35.0	39.0	33.0	39.0
13	36.62748059969232	39.0	35.0	39.0	33.0	39.0
14	37.730600543833056	40.0	35.0	41.0	33.0	41.0
15	37.903368018301855	40.0	35.0	41.0	33.0	41.0
16	38.00421044863389	40.0	35.0	41.0	33.0	41.0
17	37.913675301009896	40.0	35.0	41.0	33.0	41.0
18	37.680142145926645	39.0	36.0	41.0	33.0	41.0
19	37.41773304495422	38.0	36.0	41.0	33.0	41.0
20	37.109446686762375	38.0	35.0	41.0	33.0	41.0
21	36.99047270332714	38.0	35.0	41.0	33.0	41.0
22	36.89508676918885	38.0	35.0	41.0	33.0	41.0
23	36.862045221024424	38.0	35.0	41.0	33.0	41.0
24	36.77232184925947	38.0	35.0	41.0	33.0	41.0
25	36.75340463336683	38.0	35.0	41.0	33.0	41.0
26	36.67147828357658	38.0	35.0	41.0	33.0	41.0
27	36.59472118621457	38.0	35.0	41.0	32.0	41.0
28	36.501836997678204	38.0	35.0	41.0	32.0	41.0
29	36.388026022241526	38.0	35.0	41.0	32.0	41.0
30	36.1406603200554	38.0	35.0	41.0	31.0	41.0
31	35.75724779600016	38.0	35.0	41.0	30.0	41.0
32	35.334478902342795	38.0	35.0	41.0	27.0	41.0
33	34.85505883955789	38.0	35.0	41.0	23.0	41.0
34	34.34109402408079	38.0	34.0	41.0	19.0	41.0
35	34.06090305806752	37.0	34.0	41.0	18.0	41.0
36	33.890671957401636	37.0	34.0	40.0	16.0	41.0
37	33.82932668017734	37.0	34.0	40.0	16.0	41.0
38	33.76138160845155	37.0	33.0	40.0	15.0	41.0
39	33.68041633315735	37.0	33.0	40.0	15.0	41.0
40	33.51226519525196	37.0	33.0	40.0	15.0	41.0
41	33.38705075585983	37.0	33.0	40.0	13.0	41.0
42	33.29355007181094	36.0	33.0	40.0	12.0	41.0
43	33.16797969992677	36.0	33.0	40.0	10.0	41.0
44	33.009627207547815	36.0	33.0	40.0	10.0	41.0
45	32.959172215693954	36.0	33.0	40.0	10.0	41.0
46	32.91830185573097	36.0	33.0	40.0	10.0	41.0
47	32.90638805156764	36.0	33.0	40.0	10.0	41.0
48	32.814959382823275	35.0	33.0	40.0	10.0	41.0
49	32.74146131008135	36.0	32.0	40.0	10.0	41.0
50	32.565333764766656	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	1.0
12	6.0
13	10.0
14	21.0
15	43.0
16	123.0
17	320.0
18	695.0
19	1589.0
20	2819.0
21	4858.0
22	7585.0
23	11991.0
24	19008.0
25	31041.0
26	46731.0
27	56235.0
28	53971.0
29	47207.0
30	43614.0
31	45042.0
32	51648.0
33	65952.0
34	122648.0
35	228594.0
36	94420.0
37	121345.0
38	208360.0
39	495689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.599084907213452	22.21915677492237	24.164921064732027	22.01683725313215
2	38.56809550571365	23.005103402078827	27.466578109300226	10.960222982907293
3	26.927229687154075	23.0041383538548	39.50356784005177	10.565064118939354
4	22.995225849668195	24.936789341326204	39.80137036847812	12.266614440527485
5	19.900770335552945	31.294810878931862	38.0214240705734	10.782994714941784
6	18.874924073411787	38.008537838405516	35.25383606669051	7.862702021492192
7	76.29580431092718	3.531224986801546	18.58751000527938	1.5854606969918879
8	76.6195496063171	3.100529641172363	18.32955829175111	1.950362460759436
9	73.22314753316644	4.201592897245071	20.163660825286534	2.411598744301958
10	39.12050046265547	24.154078464097367	27.069886521682363	9.655534551564797
11	33.363533665991135	21.672655642409897	33.80132495444405	11.162485737154924
12	31.2107381483563	21.14500133403725	36.220530549453045	11.423729968153408
13	19.122771164359065	31.837962726431535	36.07611392110447	12.963152188104928
14	13.951588639679377	32.775932832643605	38.82286823685689	14.44961029082012
15	12.90621434288731	22.923414908292035	51.30661852779055	12.863752221030103
16	14.803669453953008	20.669402862219496	48.47062563508688	16.056302048740612
17	15.124349302043063	20.750410145495213	38.64637794694505	25.47886260551667
18	17.629444188990504	24.027543611664594	43.05301520802466	15.289996991320242
19	20.605879982061456	24.049172045391327	37.7329314191318	17.61201655341542
20	21.57762677611449	23.191244174230942	39.43720658276424	15.793922466890331
21	19.359832422214275	24.85549822033754	40.37653910999847	15.40813024744972
22	18.810549680114896	22.586386007936103	37.5405462172948	21.0625180946542
23	14.824049001742765	25.915915915915917	36.750966467412596	22.50906861492873
24	14.631379962192817	23.81517623483597	46.97650391412206	14.576939888849152
25	15.402850865988862	24.189160805417895	43.615695090175244	16.792293238418
26	13.637039686189025	29.25577751664708	38.2568958372361	18.850286959927793
27	14.276923426261801	31.109294549748235	38.335348581095275	16.27843344289469
28	13.18732721379224	27.177574549975308	42.76594174514779	16.86915649108466
29	14.098673342529674	24.75076210425927	40.72917908456661	20.42138546864445
30	16.321463240177795	30.531230663555803	37.56427505009736	15.583031046169044
31	19.829981209943405	28.89876644129952	36.18510760287698	15.086144745880095
32	20.749161259558235	25.691002912174937	37.081523867913276	16.478311960353548
33	21.46863309434198	28.331488388199162	32.945554249902074	17.254324267556783
34	16.996486089113688	27.940416787297696	34.82069971672996	20.242397406858654
35	18.103793774871278	26.51163450785379	34.74258757812633	20.6419841391486
36	24.96449190211005	26.978320475484935	32.44299119535414	15.614196427050869
37	16.915422038295382	30.767213338101808	36.16115169990407	16.156212923698746
38	17.268175547948704	30.118246791214652	33.45884636999949	19.15473129083715
39	17.944560817906755	27.78368160220712	35.124292534500476	19.14746504538565
40	19.99171193878188	25.45337397889383	34.17882911266654	20.376084969657747
41	16.107846977412194	26.1430428538179	35.96950447612073	21.77960569264917
42	18.471023007885012	27.131819910647888	32.53450047400898	21.86265660745812
43	18.311279143037176	26.232565268482094	33.78355671361342	21.672598874867305
44	16.115340293034055	29.3719806763285	32.93181650459533	21.58086252604211
45	16.26588781598233	32.930340548488	31.229868810209076	19.573902825320594
46	19.541829163757328	29.90639032226934	33.08849492214331	17.463285591830015
47	16.7285432880896	28.472385428907167	35.0481104923449	19.750960790658333
48	17.80298256668767	27.260341627071305	36.20389765947422	18.732778146766805
49	19.048462451108954	27.106785424365764	34.85799599221149	18.98675613231379
50	18.32461951554579	28.735332686183344	34.05342961108557	18.886618187185295
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2835.0
1	7149.5
2	11464.0
3	32945.5
4	54427.0
5	40127.5
6	25828.0
7	26613.5
8	27399.0
9	28881.5
10	30364.0
11	29708.5
12	29053.0
13	27702.0
14	26351.0
15	23349.0
16	20347.0
17	18815.5
18	17284.0
19	15691.0
20	14098.0
21	17392.0
22	20686.0
23	19140.5
24	17595.0
25	20798.0
26	24001.0
27	29025.0
28	34049.0
29	37985.0
30	41921.0
31	49008.5
32	56096.0
33	68787.5
34	81479.0
35	90321.5
36	99164.0
37	97613.5
38	96063.0
39	100909.0
40	105755.0
41	122875.5
42	139996.0
43	138003.0
44	136010.0
45	125761.0
46	115512.0
47	111380.0
48	107248.0
49	101729.5
50	96211.0
51	82926.0
52	69641.0
53	60634.5
54	51628.0
55	45878.0
56	40128.0
57	38233.0
58	36338.0
59	34347.5
60	32357.0
61	30219.5
62	28082.0
63	24585.5
64	21089.0
65	18706.0
66	16323.0
67	13859.5
68	11396.0
69	10279.5
70	9163.0
71	7493.0
72	5823.0
73	4735.5
74	3648.0
75	2886.0
76	2124.0
77	1658.5
78	1193.0
79	956.5
80	720.0
81	575.5
82	431.0
83	302.5
84	174.0
85	107.5
86	41.0
87	30.0
88	19.0
89	12.5
90	6.0
91	6.5
92	7.0
93	4.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1761570.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.5690826176546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.16491639835144	19.69578454028471
2	8.210947322663591	4.034708862794632
3	3.054664026341778	2.2515087849710578
4	1.5884882881441953	1.5611079995436585
5	1.0298792399448604	1.2651594066206302
6	0.7111751460134704	1.0483752550816519
7	0.5063424359801757	0.8708258399694996
8	0.39162665641634536	0.7697526141415217
9	0.3320702787467079	0.7342795902055914
>10	2.737314393732374	15.099879738427127
>50	0.7596012802040164	13.16565212319523
>100	0.4332508068020966	21.12788212343665
>500	0.059269825941824936	10.02171874473218
>1k	0.02022147002721086	6.676672519575439
>5k	0.0	0.0
>10k+	2.324306899679409E-4	1.6766918570204028
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29361	1.6667518179805514	No Hit
ATGTAGGGGGTATTACTAGCGTTTCTGTGGCATGATTATGCATTCTGTAA	2387	0.13550412416196914	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2152	0.12216375165335469	No Hit
AATGCAGGGATTTCGTGTTTGTTTTAACCTTGACTCTCTTTTGATTCTTT	1899	0.10780156337812292	No Hit
GGTGGGTGGATATGGGGGACTTTTGGTATAGCATTGGAAATATAAATGAG	1861	0.10564439675970867	No Hit
AGACGAGGGAAATTCCTAAACCAACAACATTTTTTGAAGATGCAGTATAG	1781	0.10110299335252075	No Hit
GGTTTGAGGAGATGAGGTAATTTTTATTATTTTAGGAGTTTCAAGCATTG	1774	0.10070562055439182	No Hit
TATGAAGGGACCAAACACAAATACGAAAAATTATAGCCTATTCATCAATT	1771	0.10053531792662228	No Hit
GTAAGAGGGCTTACTTTATTGTTGTAACCATTTCATAATGTAAATGTTTA	1766	0.10025148021367303	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09332584001771148	0.0
2	0.0	0.0	0.0	0.31829561130128237	0.0
3	0.0	0.0	0.0	0.44653349001175086	0.0
4	0.0	0.0	0.0	0.6926207871387455	0.0
5	0.0	0.0	0.0	1.1334775228915115	0.0
6	0.0	0.0	0.0	1.5309070885630431	0.0
7	0.0	0.0	0.0	1.7686495569293301	0.0
8	0.0	0.0	0.0	2.33575730740192	0.0
9	0.0	0.0	0.0	2.5719670521182807	0.0
10	0.0	0.0	0.0	3.010496318624863	0.0
11	0.0	0.0	0.0	3.539853653275203	0.0
12	0.0	0.0	0.0	3.9986489324863617	0.0
13	0.0	0.0	0.0	4.166907928722673	0.0
14	0.0	0.0	0.0	4.2304308088807145	0.0
15	0.0	0.0	0.0	4.342206100240126	0.0
16	0.0	0.0	0.0	4.5775075642750505	0.0
17	0.0	0.0	0.0	4.8575986194133645	0.0
18	0.0	0.0	0.0	5.320651464318761	0.0
19	0.0	0.0	0.0	5.509687381142958	0.0
20	0.0	0.0	0.0	5.733181196319192	0.0
21	0.0	0.0	0.0	6.011569225179811	0.0
22	0.0	0.0	0.0	6.28257747350375	0.0
23	0.0	0.0	0.0	6.589462808744472	0.0
24	0.0	0.0	0.0	6.815227325624301	0.0
25	0.0	0.0	0.0	7.003638799480009	0.0
26	0.0	0.0	0.0	7.200338334553836	0.0
27	0.0	0.0	0.0	7.426897597029922	0.0
28	0.0	0.0	0.0	7.635120943249488	0.0
29	0.0	0.0	0.0	7.895116288310995	0.0
30	0.0	0.0	0.0	8.20909756637545	0.0
31	0.0	0.0	0.0	8.500428594946554	0.0
32	0.0	0.0	0.0	8.76451120307453	0.0
33	0.0	0.0	0.0	9.014799298353173	0.0
34	0.0	0.0	0.0	9.261851643704196	0.0
35	0.0	0.0	0.0	9.558178216023206	0.0
36	0.0	0.0	0.0	9.807047122737103	0.0
37	0.0	0.0	0.0	10.07510345884637	0.0
38	0.0	0.0	0.0	10.369727004887686	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	75	0.0	44.000004	31
CGGCGTA	25	4.4438093E-5	44.0	21
CCTTACG	30	2.5284899E-6	44.0	28
ACGCCTA	25	4.4438093E-5	44.0	10
ACGTTAG	20	7.857626E-4	44.0	1
ATCGTAG	40	8.3164196E-9	44.0	1
ACGATTC	20	7.857626E-4	44.0	30
AACGCAC	30	2.5284899E-6	44.0	32
TGTTACG	30	2.5284899E-6	44.0	1
CGTACTC	20	7.857626E-4	44.0	44
ACGTCGT	20	7.857626E-4	44.0	15
ACCGTAA	25	4.4438093E-5	44.0	35
ACGTAAG	20	7.857626E-4	44.0	1
ACATCGC	20	7.857626E-4	44.0	9
ATACGAT	55	1.8189894E-12	44.0	44
GCCACTA	20	7.857626E-4	44.0	10
CGTTTCG	35	1.4466787E-7	44.0	42
CCGTTTA	25	4.4438093E-5	44.0	28
CCGTTCA	20	7.857626E-4	44.0	22
ATCGCAA	30	2.5284899E-6	44.0	14
>>END_MODULE