FastQCFastQC Report
Thu 26 May 2016
SRR1546256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546256_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1786169
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297961.668151221972837No Hit
ATGTAGGGGGTATTACTAGCGTTTCTGTGGCATGATTATGCATTCTGTAA24110.13498162827817525No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23180.12977495410568654No Hit
AATGCAGGGATTTCGTGTTTGTTTTAACCTTGACTCTCTTTTGATTCTTT19670.11012395803532589No Hit
ATTATGGGGAGTTAGATGAAGTTTTTCTGTCTGCCTGTCTGTCTGTCTCT18730.10486129811904696No Hit
AGACGAGGGAAATTCCTAAACCAACAACATTTTTTGAAGATGCAGTATAG18610.10418946919356455No Hit
GGTGGGTGGATATGGGGGACTTTTGGTATAGCATTGGAAATATAAATGAG18600.10413348344977436No Hit
TATGAAGGGACCAAACACAAATACGAAAAATTATAGCCTATTCATCAATT18390.10295778283018013No Hit
GGTTTGAGGAGATGAGGTAATTTTTATTATTTTAGGAGTTTCAAGCATTG18260.10222996816090751No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGAG302.5285062E-644.0000041
TTCGTAG600.044.0000041
ATGCGTA600.044.00000411
ACGTAAG302.5285062E-644.0000041
TGCGTAA600.044.00000412
TTGATCG207.8576565E-444.020
TAATACG207.8576565E-444.01
CGCATCG408.3164196E-944.021
CGTTCGC351.4466787E-744.040
CGTTCGA254.4438348E-544.014
GTTACGA454.802132E-1044.038
TACGATC454.802132E-1044.040
CTACGAT207.8576565E-444.031
CGAATTA502.7284841E-1144.025
GTGTACG408.3164196E-944.01
CTTTACG351.4466787E-744.035
ATACGGT207.8576565E-444.03
CGATCAA454.802132E-1044.042
CCGCAAT254.4438348E-544.033
TACGTAG351.4466787E-744.01
Produced by FastQC (version 0.11.5)