##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546256_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1786169
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.870964617569783	31.0	31.0	34.0	30.0	34.0
2	32.254704342086335	33.0	31.0	34.0	30.0	34.0
3	32.25768950194522	34.0	31.0	34.0	30.0	34.0
4	35.95946240249383	37.0	35.0	37.0	35.0	37.0
5	35.90088227933639	37.0	35.0	37.0	35.0	37.0
6	35.975651240168204	37.0	35.0	37.0	35.0	37.0
7	36.1935186424129	37.0	35.0	37.0	35.0	37.0
8	36.10599444957336	37.0	35.0	37.0	35.0	37.0
9	37.85521806727135	39.0	38.0	39.0	35.0	39.0
10	37.47647171124345	39.0	37.0	39.0	35.0	39.0
11	37.3234106067231	39.0	37.0	39.0	34.0	39.0
12	36.7914206326501	39.0	35.0	39.0	33.0	39.0
13	36.582285886721806	39.0	35.0	39.0	33.0	39.0
14	37.6751847109652	40.0	35.0	41.0	33.0	41.0
15	37.87994920973323	40.0	35.0	41.0	33.0	41.0
16	37.973554014205824	40.0	35.0	41.0	33.0	41.0
17	37.89155505442094	40.0	35.0	41.0	33.0	41.0
18	37.674905342103685	39.0	36.0	41.0	33.0	41.0
19	37.415445011082376	38.0	36.0	41.0	33.0	41.0
20	37.1066035744658	38.0	35.0	41.0	33.0	41.0
21	36.98554448095337	38.0	35.0	41.0	33.0	41.0
22	36.89987677537792	38.0	35.0	41.0	33.0	41.0
23	36.84806252935753	38.0	35.0	41.0	33.0	41.0
24	36.76746545259715	38.0	35.0	41.0	33.0	41.0
25	36.73729697469836	38.0	35.0	41.0	33.0	41.0
26	36.67509513377514	38.0	35.0	41.0	33.0	41.0
27	36.59150953801124	38.0	35.0	41.0	32.0	41.0
28	36.488097710798925	38.0	35.0	41.0	32.0	41.0
29	36.37532730665463	38.0	35.0	41.0	32.0	41.0
30	36.13356071010078	38.0	35.0	41.0	31.0	41.0
31	35.75858219462996	38.0	35.0	41.0	30.0	41.0
32	35.34252805865514	38.0	35.0	41.0	27.0	41.0
33	34.8564212009054	38.0	35.0	41.0	23.0	41.0
34	34.40831746604045	38.0	34.0	41.0	20.0	41.0
35	34.091874285132036	37.0	34.0	41.0	18.0	41.0
36	33.91571626201104	37.0	34.0	40.0	17.0	41.0
37	33.796898837679976	37.0	33.0	40.0	16.0	41.0
38	33.73440811031879	37.0	33.0	40.0	15.0	41.0
39	33.65934634404695	37.0	33.0	40.0	15.0	41.0
40	33.501632264360204	37.0	33.0	40.0	15.0	41.0
41	33.396609727299044	37.0	33.0	40.0	13.0	41.0
42	33.28975533670106	36.0	33.0	40.0	12.0	41.0
43	33.18571870858805	36.0	33.0	40.0	11.0	41.0
44	33.04700115162675	36.0	33.0	40.0	10.0	41.0
45	32.99119904107618	36.0	33.0	40.0	10.0	41.0
46	32.954883888366666	36.0	33.0	40.0	10.0	41.0
47	32.918255215491925	36.0	33.0	40.0	10.0	41.0
48	32.84057219669583	35.0	33.0	40.0	10.0	41.0
49	32.76168044569131	36.0	32.0	40.0	10.0	41.0
50	32.562635450508886	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	3.0
12	7.0
13	5.0
14	19.0
15	49.0
16	122.0
17	328.0
18	782.0
19	1571.0
20	2861.0
21	4855.0
22	7774.0
23	12044.0
24	19127.0
25	30754.0
26	46912.0
27	56673.0
28	55197.0
29	48429.0
30	44906.0
31	45666.0
32	52472.0
33	67498.0
34	127298.0
35	228641.0
36	96279.0
37	123392.0
38	211385.0
39	501118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.829183016836595	22.265586291106832	24.05309911884038	21.852131573216198
2	38.62506851255396	23.083705965113044	27.336607006391894	10.9546185159411
3	26.94375504221605	23.002582622361043	39.515017895843	10.538644439579906
4	23.0685338285459	24.964267099025903	39.678888167916924	12.288310904511276
5	19.985846803969835	31.167879411186732	38.00866547342385	10.837608311419581
6	18.841442215154334	38.024901339123005	35.28400728038612	7.8496491653365394
7	76.27805655567866	3.517192382131814	18.602663017889125	1.6020880443003993
8	76.55563387339048	3.12389253200565	18.335107148315753	1.985366446288117
9	73.14464644722868	4.226195841490923	20.16931208637033	2.4598456249100726
10	39.0699312327109	24.168989608486093	27.05667828744089	9.704400871362116
11	33.33570339648712	21.691004602587995	33.77183234061278	11.201459660312098
12	31.15309917482612	21.192395568392463	36.23794836882736	11.416556887954052
13	19.19919111797372	31.723202003841745	36.13818177339322	12.939425104791317
14	14.04760691737456	32.658387868113266	38.88551419266598	14.408491021846197
15	12.901018884551238	22.97772495211819	51.241847775882356	12.87940838744822
16	14.872780795098336	20.78526723955012	48.34570524961524	15.996246715736303
17	15.18031048573791	20.697761522006036	38.59869922722878	25.523228765027273
18	17.66383808027124	24.05920156491351	43.013902939755425	15.26305741505983
19	20.692218933370807	24.057018120905692	37.72772901108461	17.523033934638885
20	21.606578100952373	23.231676285950545	39.43826144110664	15.723484171990446
21	19.312226334686137	24.83538791682086	40.39746518946416	15.454920559028848
22	18.787136043677837	22.50940420531316	37.63445676193014	21.06900298907886
23	14.859176259357318	25.972234430224688	36.69115296480904	22.477436345608954
24	14.672967675511108	23.835538518471655	46.822053232364915	14.669440573652325
25	15.424408328663189	24.163670962826025	43.60791168136945	16.80400902714133
26	13.638183173036818	29.20580303431534	38.286466734110824	18.869547058537016
27	14.213940562175248	31.079142007279266	38.451120806597814	16.255796623947678
28	13.141869554336683	27.133434742177254	42.80278069992257	16.92191500356349
29	14.164785079127451	24.7597511769603	40.77217777265197	20.30328597126028
30	16.28944405596559	30.527010602020297	37.608815291274226	15.574730050739879
31	19.813858599046338	28.79985040609259	36.23582091056333	15.150470084297735
32	20.6859485300663	25.77740404183479	37.08574048704238	16.450906941056527
33	21.506867491262025	28.317925123546537	32.91390680277174	17.261300582419693
34	16.933392081040484	28.011459162038975	34.68148870571597	20.373660051204563
35	18.096663865513285	26.57128188877984	34.655287377622166	20.676766868084712
36	25.00530464922412	26.97650670233332	32.40717983572663	15.611008812715928
37	16.90148020708007	30.75425673606473	36.166846474213806	16.177416582641396
38	17.23885029915982	30.09827177607494	33.51161060347593	19.151267321289307
39	17.96996812731606	27.80341613811459	35.091360336004044	19.13525539856531
40	19.991221435373696	25.459964874544344	34.18747050251124	20.361343187570714
41	16.115664307240802	26.122556152301378	35.95768373541362	21.804095805044206
42	18.442991676599473	27.115407332676806	32.59517996337413	21.846421027349596
43	18.317247696046678	26.149373323576885	33.78437314722179	21.749005833154644
44	16.112921005795084	29.359427915275653	32.917769819093266	21.609881259835994
45	16.310158781167964	32.88832131785962	31.189713851264916	19.611806049707504
46	19.562986481122447	29.951701098832196	33.03589973849059	17.44941268155477
47	16.70407447447582	28.513987198299823	34.910918283768225	19.871020043456134
48	17.787790517022746	27.31135743594251	36.165110916156316	18.735741130878434
49	19.089683003120086	27.02258296947265	34.85409275382116	19.033641273586095
50	18.346640211536535	28.759260741844695	33.98301056618943	18.911088480429346
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2766.0
1	7318.0
2	11870.0
3	33625.0
4	55380.0
5	40511.0
6	25642.0
7	26664.5
8	27687.0
9	29166.0
10	30645.0
11	30129.5
12	29614.0
13	28026.5
14	26439.0
15	23545.5
16	20652.0
17	19010.5
18	17369.0
19	15848.0
20	14327.0
21	17664.0
22	21001.0
23	19467.0
24	17933.0
25	20982.5
26	24032.0
27	29230.5
28	34429.0
29	38465.5
30	42502.0
31	50199.5
32	57897.0
33	69980.5
34	82064.0
35	90820.0
36	99576.0
37	98263.5
38	96951.0
39	102087.0
40	107223.0
41	124034.0
42	140845.0
43	139519.0
44	138193.0
45	127531.5
46	116870.0
47	113205.0
48	109540.0
49	104319.5
50	99099.0
51	85485.0
52	71871.0
53	62575.0
54	53279.0
55	46896.0
56	40513.0
57	38533.5
58	36554.0
59	34833.0
60	33112.0
61	30563.0
62	28014.0
63	24664.0
64	21314.0
65	18815.5
66	16317.0
67	13885.5
68	11454.0
69	10304.0
70	9154.0
71	7398.5
72	5643.0
73	4659.0
74	3675.0
75	2930.5
76	2186.0
77	1660.5
78	1135.0
79	921.0
80	707.0
81	563.5
82	420.0
83	301.0
84	182.0
85	117.0
86	52.0
87	38.5
88	25.0
89	17.5
90	10.0
91	6.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1786169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.585855311454964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.96464177400796	19.65999112688087
2	8.249134774645906	4.056240680282718
3	3.160588339487375	2.3311730284112486
4	1.661290401748214	1.6337698179076185
5	1.0367835436626482	1.2745105096893707
6	0.7036734151714471	1.0380247663153546
7	0.5311578570976996	0.914127915550256
8	0.3910469581012207	0.7691379145488962
9	0.3030946494162176	0.6706657076600965
>10	2.733096036910899	15.108068761439347
>50	0.7521974889112156	12.974172289166827
>100	0.43328588581770966	21.08519592285622
>500	0.0589177102593962	9.916765674692229
>1k	0.020861912971225888	6.888748633141893
>5k	0.0	0.0
>10k+	2.292517908925922E-4	1.6794072514571747
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29796	1.668151221972837	No Hit
ATGTAGGGGGTATTACTAGCGTTTCTGTGGCATGATTATGCATTCTGTAA	2411	0.13498162827817525	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2318	0.12977495410568654	No Hit
AATGCAGGGATTTCGTGTTTGTTTTAACCTTGACTCTCTTTTGATTCTTT	1967	0.11012395803532589	No Hit
ATTATGGGGAGTTAGATGAAGTTTTTCTGTCTGCCTGTCTGTCTGTCTCT	1873	0.10486129811904696	No Hit
AGACGAGGGAAATTCCTAAACCAACAACATTTTTTGAAGATGCAGTATAG	1861	0.10418946919356455	No Hit
GGTGGGTGGATATGGGGGACTTTTGGTATAGCATTGGAAATATAAATGAG	1860	0.10413348344977436	No Hit
TATGAAGGGACCAAACACAAATACGAAAAATTATAGCCTATTCATCAATT	1839	0.10295778283018013	No Hit
GGTTTGAGGAGATGAGGTAATTTTTATTATTTTAGGAGTTTCAAGCATTG	1826	0.10222996816090751	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09539970741850295	0.0
2	0.0	0.0	0.0	0.3251651999334889	0.0
3	0.0	0.0	0.0	0.4604267569306152	0.0
4	0.0	0.0	0.0	0.7117467608048287	0.0
5	0.0	0.0	0.0	1.1584010247630543	0.0
6	0.0	0.0	0.0	1.5503012312944633	0.0
7	0.0	0.0	0.0	1.7893043715348325	0.0
8	0.0	0.0	0.0	2.357727628236746	0.0
9	0.0	0.0	0.0	2.599753998641786	0.0
10	0.0	0.0	0.0	3.0596768838782893	0.0
11	0.0	0.0	0.0	3.607553372609199	0.0
12	0.0	0.0	0.0	4.093229699989195	0.0
13	0.0	0.0	0.0	4.266673534251238	0.0
14	0.0	0.0	0.0	4.3348641701877035	0.0
15	5.5985743790201264E-5	0.0	0.0	4.450642688345839	0.0
16	5.5985743790201264E-5	0.0	0.0	4.691773286850236	0.0
17	5.5985743790201264E-5	0.0	0.0	4.977356565924053	0.0
18	5.5985743790201264E-5	0.0	0.0	5.455922703842694	0.0
19	5.5985743790201264E-5	0.0	0.0	5.658647082107012	0.0
20	5.5985743790201264E-5	0.0	0.0	5.899497751892458	0.0
21	5.5985743790201264E-5	0.0	0.0	6.1943186786916575	0.0
22	5.5985743790201264E-5	0.0	0.0	6.477046684832175	0.0
23	5.5985743790201264E-5	0.0	0.0	6.7936460659657625	0.0
24	5.5985743790201264E-5	0.0	0.0	7.036568208271445	0.0
25	5.5985743790201264E-5	0.0	0.0	7.230726767735864	0.0
26	5.5985743790201264E-5	0.0	0.0	7.447167653228782	0.0
27	1.1197148758040253E-4	0.0	0.0	7.688914094914871	0.0
28	1.1197148758040253E-4	0.0	0.0	7.907874338878348	0.0
29	1.1197148758040253E-4	0.0	0.0	8.180412939649047	0.0
30	1.1197148758040253E-4	0.0	0.0	8.503562652806089	0.0
31	1.1197148758040253E-4	0.0	0.0	8.807509255843092	0.0
32	1.1197148758040253E-4	0.0	0.0	9.068850707855752	0.0
33	1.1197148758040253E-4	0.0	0.0	9.32408971379528	0.0
34	1.1197148758040253E-4	0.0	0.0	9.59147762613728	0.0
35	1.1197148758040253E-4	0.0	0.0	9.912107980823762	0.0
36	1.1197148758040253E-4	0.0	0.0	10.170818102878282	0.0
37	1.1197148758040253E-4	0.0	0.0	10.448899292284212	0.0
38	1.1197148758040253E-4	0.0	0.0	10.750102593875496	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACGAG	30	2.5285062E-6	44.000004	1
TTCGTAG	60	0.0	44.000004	1
ATGCGTA	60	0.0	44.000004	11
ACGTAAG	30	2.5285062E-6	44.000004	1
TGCGTAA	60	0.0	44.000004	12
TTGATCG	20	7.8576565E-4	44.0	20
TAATACG	20	7.8576565E-4	44.0	1
CGCATCG	40	8.3164196E-9	44.0	21
CGTTCGC	35	1.4466787E-7	44.0	40
CGTTCGA	25	4.4438348E-5	44.0	14
GTTACGA	45	4.802132E-10	44.0	38
TACGATC	45	4.802132E-10	44.0	40
CTACGAT	20	7.8576565E-4	44.0	31
CGAATTA	50	2.7284841E-11	44.0	25
GTGTACG	40	8.3164196E-9	44.0	1
CTTTACG	35	1.4466787E-7	44.0	35
ATACGGT	20	7.8576565E-4	44.0	3
CGATCAA	45	4.802132E-10	44.0	42
CCGCAAT	25	4.4438348E-5	44.0	33
TACGTAG	35	1.4466787E-7	44.0	1
>>END_MODULE