SRR1546255_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1546255_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	822069
Sequences flagged as poor quality	0
Sequence length	50
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11044	1.3434395409631066	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1413	0.17188338205187156	No Hit
AAGCTTGGGCAAGAGTTACAAAAATACACTCTGACATGGGAAGCAGTTTA	1233	0.14998740981596437	No Hit
TGGTAAGGGAACAGAATTTAACTGGATCGCAGACAGTTATTACTGTAATT	1138	0.13843120224701333	No Hit
GAGTGAGGGATTTATTAGTTGATTTTATTGTTTAGAAAGGCACATGCATG	1105	0.13441694067043033	No Hit
TGGTTTGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA	1052	0.12796979328985766	No Hit
GTTGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1050	0.1277265047094587	No Hit
TATTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1045	0.12711828325846125	No Hit
TTTAATGGGTATCAACAATAATTTTAAATAATCTAAGTATAGGTAATATA	1037	0.1261451289368654	No Hit
GCAATAGGGATATCTTATCATTTGGTTTTTAATATTCGATAAATCCATAA	1001	0.12176593448968397	No Hit
GGGTTTGGGAAGTGTATACTTTATCTTCACATGTGGGTGGAAGGCATTGA	984	0.11969798155629273	No Hit
GGATTGGGGATAACATTTTAAATGTAAATAAAGAAAATATCCAACAAAAA	971	0.11811660578369942	No Hit
ACTGGAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	968	0.11775167291310097	No Hit
TTGGTAGGGCATATTTTAAAATTTAGAAGAAAACTTAGAGAAAACTCCAT	955	0.11617029714050767	No Hit
TGTAGAGGGAGCTGTTAAGAGCAAGTATTTAATTCAGGTATTTTTCAAGT	941	0.1144672770777149	No Hit
TAAGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	938	0.11410234420711643	No Hit
GTTACTGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	922	0.11215603556392467	No Hit
TGGAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	921	0.1120343912737252	No Hit
ATGACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	914	0.1111828812423288	No Hit
ATGAGAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	909	0.11057465979133138	No Hit
GACTGAGGGACTGCTAAACTGTAAAATTCAGGAATTAAAATGTGACCCTA	909	0.11057465979133138	No Hit
AAATTGGGGAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGG	885	0.10765519682654374	No Hit
TTTTGAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	879	0.10692533108534684	No Hit
TCTTTAGGGCGATTGGAATGGGTTTTCAAAACAATCACCATCATGCTATT	878	0.10680368679514736	No Hit
CTGCAAGGGATGAATAATTATATTTTTGTTTTAAGTTCATTATTTTTGGT	877	0.1066820425049479	No Hit
CTGGAGGGGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTTTA	872	0.10607382105395045	No Hit
GAGCTAGGGATTCTATTAAATATATGGAGAATATATTTTGGAGTACCATG	851	0.10351929095976128	No Hit
TGTGAGGGGTGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTA	827	0.10059982799497365	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	40	8.307325E-9	44.000004	22
TACCCGA	20	7.8551704E-4	44.000004	25
GAAGCGC	20	7.8551704E-4	44.000004	9
ACCGGAC	20	7.8551704E-4	44.000004	13
CAAGTTC	20	7.8551704E-4	44.000004	35
TCCGATA	20	7.8551704E-4	44.000004	36
ACTTGAT	20	7.8551704E-4	44.000004	34
CCGGCAT	20	7.8551704E-4	44.000004	29
CATTGCG	20	7.8551704E-4	44.000004	1
ATTCGCA	20	7.8551704E-4	44.000004	20
AAGCGTG	20	7.8551704E-4	44.000004	34
CGCTGTT	20	7.8551704E-4	44.000004	17
TGGCCAT	20	7.8551704E-4	44.000004	44
TGTTGGC	20	7.8551704E-4	44.000004	11
ACGATTG	20	7.8551704E-4	44.000004	1
GTTACGA	20	7.8551704E-4	44.000004	9
GGCCACT	20	7.8551704E-4	44.000004	8
CCGTAGG	20	7.8551704E-4	44.000004	2
TACGCGG	20	7.8551704E-4	44.000004	2
GGGACGT	40	8.307325E-9	44.000004	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content