##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546255_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 822069 Sequences flagged as poor quality 0 Sequence length 50 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.868012295804853 31.0 31.0 34.0 30.0 34.0 2 32.23772092123654 33.0 31.0 34.0 30.0 34.0 3 32.12047042279906 33.0 31.0 34.0 30.0 34.0 4 35.86751355421504 37.0 35.0 37.0 35.0 37.0 5 35.87380378046125 37.0 35.0 37.0 35.0 37.0 6 35.934257343361686 37.0 35.0 37.0 35.0 37.0 7 36.180840051139256 37.0 35.0 37.0 35.0 37.0 8 36.07759445983245 37.0 35.0 37.0 35.0 37.0 9 37.85629186844413 39.0 38.0 39.0 35.0 39.0 10 37.504179089589805 39.0 37.0 39.0 35.0 39.0 11 37.27265959426764 39.0 37.0 39.0 34.0 39.0 12 36.533456437355014 39.0 35.0 39.0 33.0 39.0 13 36.31141059935358 39.0 35.0 39.0 33.0 39.0 14 37.43249654226105 40.0 35.0 41.0 33.0 41.0 15 37.648595190914634 40.0 35.0 41.0 33.0 41.0 16 37.78732320522973 40.0 35.0 41.0 33.0 41.0 17 37.72996913884358 40.0 35.0 41.0 34.0 41.0 18 37.54726671362136 39.0 36.0 41.0 33.0 41.0 19 37.2756873206507 38.0 35.0 41.0 33.0 41.0 20 36.90590449220199 37.0 35.0 41.0 33.0 41.0 21 36.828070393117855 37.0 35.0 41.0 33.0 41.0 22 36.7563245907582 37.0 35.0 41.0 33.0 41.0 23 36.727718719474886 37.0 35.0 41.0 33.0 41.0 24 36.67989426678296 37.0 35.0 41.0 33.0 41.0 25 36.63575320319827 37.0 35.0 41.0 33.0 41.0 26 36.545133072771264 37.0 35.0 41.0 33.0 41.0 27 36.48054360400404 37.0 35.0 41.0 32.0 41.0 28 36.42508962143081 37.0 35.0 41.0 32.0 41.0 29 36.33796433146123 37.0 35.0 41.0 32.0 41.0 30 36.134676043008554 37.0 35.0 40.0 31.0 41.0 31 35.759988516779 37.0 35.0 40.0 30.0 41.0 32 35.3220958338047 37.0 35.0 41.0 29.0 41.0 33 34.852025803186834 37.0 35.0 41.0 24.0 41.0 34 34.326027377263955 36.0 34.0 40.0 21.0 41.0 35 34.05824450259041 37.0 34.0 40.0 18.0 41.0 36 33.87653104544752 36.0 34.0 40.0 18.0 41.0 37 33.827671399846 36.0 34.0 40.0 18.0 41.0 38 33.798092374241094 36.0 33.0 40.0 18.0 41.0 39 33.72109640431643 36.0 34.0 40.0 17.0 41.0 40 33.58277103260188 36.0 33.0 40.0 16.0 41.0 41 33.47779444304553 36.0 33.0 40.0 15.0 41.0 42 33.387665755550934 36.0 33.0 40.0 15.0 41.0 43 33.24696102152009 35.0 33.0 40.0 12.0 41.0 44 33.095045549704466 35.0 33.0 40.0 11.0 41.0 45 33.039104989970426 35.0 33.0 40.0 11.0 41.0 46 32.98401594026779 35.0 33.0 40.0 10.0 41.0 47 32.95497944819717 35.0 33.0 40.0 10.0 41.0 48 32.87159836947993 35.0 33.0 40.0 10.0 41.0 49 32.836881089057 36.0 33.0 40.0 10.0 41.0 50 32.636563354171 35.0 32.0 40.0 10.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 0.0 10 1.0 11 1.0 12 2.0 13 5.0 14 13.0 15 12.0 16 45.0 17 152.0 18 361.0 19 714.0 20 1376.0 21 2091.0 22 3313.0 23 5219.0 24 8507.0 25 14188.0 26 21741.0 27 25990.0 28 24780.0 29 21235.0 30 20037.0 31 20699.0 32 24484.0 33 31674.0 34 59751.0 35 127889.0 36 42101.0 37 52445.0 38 89371.0 39 223871.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.523115456245158 24.50426910636455 27.50645018848783 20.466165248902467 2 37.10929374541553 22.27975997148658 29.46978903230751 11.141157250790384 3 25.237054310526247 23.74180269539418 40.19297650197246 10.82816649210711 4 21.37095547940623 24.787092081078352 41.99124404399144 11.850708395523975 5 19.526341462821247 29.652133823316536 38.66098831120016 12.160536402662062 6 16.497520281144283 40.03204110603855 35.16298510222378 8.307453510593394 7 77.64031972985237 3.6614931350044824 17.2485521288359 1.4496350063072565 8 78.08955209355905 3.1181080906833856 16.570263566683575 2.2220762490739827 9 75.47188861275636 3.9091609098506326 17.999462332237318 2.6194881451556986 10 43.46946545849558 24.12473892094216 23.085531749767963 9.320263870794301 11 37.43128618157356 19.346064624745612 31.71534262938999 11.507306564290833 12 34.024637834537 18.74927773702694 36.159616771828155 11.0664676566079 13 16.03588019983724 38.07867709401522 34.49102204316183 11.394420662985711 14 10.731094348527922 39.21191530151362 38.19375259254393 11.863237757414524 15 9.618535670363437 21.318161857459653 57.182304648393256 11.880997823783648 16 10.947134607922205 18.458912816320773 52.82585768347913 17.7680948922779 17 10.895314140297225 20.169353180815722 38.304448896625466 30.630883782261588 18 14.787323205229733 23.686089610482817 45.16044273655861 16.36614444772884 19 19.56818709864987 24.46400484630852 36.87111422520494 19.09669382983667 20 22.358585471535843 21.127301966136663 39.13479282152715 17.379319740800348 21 16.020431374981907 26.127003937625677 39.83461242304478 18.017952264347638 22 18.123174575370193 22.026131626420653 34.09300192562911 25.75769187258004 23 12.514156354271966 26.767947702686754 35.17879885994971 25.539097083091566 24 13.252293907202436 21.185812869722614 51.28352972803986 14.278363495035087 25 12.196421468270911 25.418425947213674 44.86813150720925 17.517021077306165 26 13.102306497386474 30.073996221728343 37.94474673050559 18.878950550379592 27 12.199827508396497 34.625682272412654 34.93100944081336 18.243480778377485 28 11.270343486982236 28.854512212478518 41.26271638998673 18.61242791055252 29 11.09712201773817 23.380762442082112 41.11686488603755 24.405250654142172 30 13.507138695170356 30.929033937540524 38.54542623551065 17.018401131778475 31 18.8299279014292 31.22365640840367 33.51093399702458 16.435481693142545 32 20.268249988747904 24.758748961461873 37.048228311735386 17.924772738054834 33 21.96993196434849 28.91217160603307 31.818010410318355 17.299886019300086 34 15.630439780602359 26.72220944957175 33.27628216122977 24.371068608596115 35 16.124558887392666 26.688757269766896 35.835556382736726 21.351127460103715 36 24.461815249084932 26.903094509098384 31.60878223117524 17.02630801064144 37 15.418657071365056 32.37939880958898 36.59315702209912 15.60878709694685 38 16.47951692619476 28.72228486903167 34.02378632450561 20.774411880267955 39 16.258124318031697 31.816429034545763 34.252112657210034 17.6733339902125 40 18.407700570146787 25.67047291650701 33.943501092973946 21.978325420372254 41 12.813401308162698 26.768190991267154 36.351328173182544 24.0670795273876 42 16.976677140240053 27.797423330644992 31.992083389593816 23.233816139521135 43 17.92744891243922 25.331206991140647 34.536881940567035 22.204462155853097 44 14.693292168905531 28.040955199624364 35.53947418039118 21.726278451078922 45 14.82041045216399 35.71865621985502 30.224835141575706 19.23609818640528 46 20.015594798003576 29.559319229894328 33.10926455078588 17.315821421316215 47 17.625406139873903 29.025787373079385 32.33098438208958 21.017822104957126 48 17.32202528011639 27.29357268063873 34.32558580849053 21.058816230754353 49 19.168099028183764 25.047775794975845 36.20134076336658 19.58278441347381 50 18.2158675245022 30.487343519826194 32.87047680912429 18.42631214654731 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1897.0 1 2449.0 2 3001.0 3 11213.0 4 19425.0 5 14739.5 6 10054.0 7 11167.5 8 12281.0 9 12959.0 10 13637.0 11 13655.5 12 13674.0 13 12927.0 14 12180.0 15 11350.5 16 10521.0 17 9747.5 18 8974.0 19 7876.5 20 6779.0 21 6524.0 22 6269.0 23 7424.0 24 8579.0 25 9556.5 26 10534.0 27 12877.5 28 15221.0 29 18865.0 30 22509.0 31 24249.5 32 25990.0 33 31089.5 34 36189.0 35 38922.0 36 41655.0 37 45889.0 38 50123.0 39 52464.5 40 54806.0 41 67139.5 42 79473.0 43 75482.5 44 71492.0 45 67708.0 46 63924.0 47 60479.5 48 57035.0 49 51354.5 50 45674.0 51 38669.5 52 31665.0 53 26325.0 54 20985.0 55 18091.5 56 15198.0 57 13563.0 58 11928.0 59 11480.5 60 11033.0 61 10408.0 62 9783.0 63 8499.5 64 7216.0 65 5646.5 66 4077.0 67 3580.5 68 3084.0 69 2569.5 70 2055.0 71 1658.0 72 1261.0 73 1078.0 74 895.0 75 691.0 76 487.0 77 383.0 78 279.0 79 217.5 80 156.0 81 92.5 82 29.0 83 25.0 84 21.0 85 15.0 86 9.0 87 8.0 88 7.0 89 4.0 90 1.0 91 1.0 92 1.0 93 1.5 94 2.0 95 1.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 822069.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.346804843583946 #Duplication Level Percentage of deduplicated Percentage of total 1 79.86867521588002 20.24415723812501 2 8.415329682655505 4.266034383213766 3 3.1483181001681064 2.3939941341145192 4 1.5406282178755974 1.562000111000452 5 1.0005383321423322 1.2680224921668333 6 0.6312131831906833 0.9599542421418993 7 0.47003755593375246 0.8339765139585598 8 0.3554507529074857 0.7207632692360816 9 0.2863681396530947 0.6532665614286467 >10 3.1043264829516843 18.274810094380236 >50 0.6582785560393846 11.460999882963092 >100 0.44801522238850733 23.281252319282896 >500 0.06799800468975191 11.499382088388375 >1k 0.004340298171686292 1.2314087181336646 >5k 0.0 0.0 >10k+ 4.8225535240958807E-4 1.349977951465971 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11044 1.3434395409631066 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1413 0.17188338205187156 No Hit AAGCTTGGGCAAGAGTTACAAAAATACACTCTGACATGGGAAGCAGTTTA 1233 0.14998740981596437 No Hit TGGTAAGGGAACAGAATTTAACTGGATCGCAGACAGTTATTACTGTAATT 1138 0.13843120224701333 No Hit GAGTGAGGGATTTATTAGTTGATTTTATTGTTTAGAAAGGCACATGCATG 1105 0.13441694067043033 No Hit TGGTTTGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA 1052 0.12796979328985766 No Hit GTTGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 1050 0.1277265047094587 No Hit TATTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 1045 0.12711828325846125 No Hit TTTAATGGGTATCAACAATAATTTTAAATAATCTAAGTATAGGTAATATA 1037 0.1261451289368654 No Hit GCAATAGGGATATCTTATCATTTGGTTTTTAATATTCGATAAATCCATAA 1001 0.12176593448968397 No Hit GGGTTTGGGAAGTGTATACTTTATCTTCACATGTGGGTGGAAGGCATTGA 984 0.11969798155629273 No Hit GGATTGGGGATAACATTTTAAATGTAAATAAAGAAAATATCCAACAAAAA 971 0.11811660578369942 No Hit ACTGGAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA 968 0.11775167291310097 No Hit TTGGTAGGGCATATTTTAAAATTTAGAAGAAAACTTAGAGAAAACTCCAT 955 0.11617029714050767 No Hit TGTAGAGGGAGCTGTTAAGAGCAAGTATTTAATTCAGGTATTTTTCAAGT 941 0.1144672770777149 No Hit TAAGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 938 0.11410234420711643 No Hit GTTACTGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA 922 0.11215603556392467 No Hit TGGAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA 921 0.1120343912737252 No Hit ATGACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA 914 0.1111828812423288 No Hit ATGAGAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA 909 0.11057465979133138 No Hit GACTGAGGGACTGCTAAACTGTAAAATTCAGGAATTAAAATGTGACCCTA 909 0.11057465979133138 No Hit AAATTGGGGAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGG 885 0.10765519682654374 No Hit TTTTGAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC 879 0.10692533108534684 No Hit TCTTTAGGGCGATTGGAATGGGTTTTCAAAACAATCACCATCATGCTATT 878 0.10680368679514736 No Hit CTGCAAGGGATGAATAATTATATTTTTGTTTTAAGTTCATTATTTTTGGT 877 0.1066820425049479 No Hit CTGGAGGGGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTTTA 872 0.10607382105395045 No Hit GAGCTAGGGATTCTATTAAATATATGGAGAATATATTTTGGAGTACCATG 851 0.10351929095976128 No Hit TGTGAGGGGTGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTA 827 0.10059982799497365 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.08916526471622212 0.0 2 0.0 0.0 0.0 0.39278941305413534 0.0 3 0.0 0.0 0.0 0.5876635659537095 0.0 4 0.0 0.0 0.0 0.9618414026073238 0.0 5 0.0 0.0 0.0 1.6640938899289475 0.0 6 0.0 0.0 0.0 2.2811953741109323 0.0 7 0.0 0.0 0.0 2.632382439916844 0.0 8 0.0 0.0 0.0 3.4872985114388206 0.0 9 0.0 0.0 0.0 3.786421821039353 0.0 10 0.0 0.0 0.0 4.361556025102516 0.0 11 0.0 0.0 0.0 4.974399959127519 0.0 12 0.0 0.0 0.0 5.528854633856769 0.0 13 0.0 0.0 0.0 5.7250668739485375 0.0 14 0.0 0.0 0.0 5.788443549142468 0.0 15 0.0 0.0 0.0 5.900964517576992 0.0 16 0.0 0.0 0.0 6.157269037027305 0.0 17 0.0 0.0 0.0 6.481572714699131 0.0 18 0.0 0.0 0.0 7.024106248988832 0.0 19 0.0 0.0 0.0 7.22518426068858 0.0 20 0.0 0.0 0.0 7.504357906696397 0.0 21 0.0 0.0 0.0 7.823187591309245 0.0 22 0.0 0.0 0.0 8.111241270501624 0.0 23 0.0 0.0 0.0 8.477512228292273 0.0 24 0.0 0.0 0.0 8.734060036322985 0.0 25 0.0 0.0 0.0 8.945964389850486 0.0 26 0.0 0.0 0.0 9.168573440915544 0.0 27 0.0 0.0 0.0 9.37013802977609 0.0 28 0.0 0.0 0.0 9.625955972065606 0.0 29 0.0 0.0 0.0 9.851849418966047 0.0 30 0.0 0.0 0.0 10.190750411461812 0.0 31 0.0 0.0 0.0 10.541694188687325 0.0 32 0.0 0.0 0.0 10.804202566937812 0.0 33 0.0 0.0 0.0 11.059047354905731 0.0 34 0.0 0.0 0.0 11.310729391328465 0.0 35 0.0 0.0 0.0 11.672377866091532 0.0 36 0.0 0.0 0.0 11.963472652538899 0.0 37 0.0 0.0 0.0 12.247390425864495 0.0 38 0.0 0.0 0.0 12.485691590365285 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGA 40 8.307325E-9 44.000004 22 TACCCGA 20 7.8551704E-4 44.000004 25 GAAGCGC 20 7.8551704E-4 44.000004 9 ACCGGAC 20 7.8551704E-4 44.000004 13 CAAGTTC 20 7.8551704E-4 44.000004 35 TCCGATA 20 7.8551704E-4 44.000004 36 ACTTGAT 20 7.8551704E-4 44.000004 34 CCGGCAT 20 7.8551704E-4 44.000004 29 CATTGCG 20 7.8551704E-4 44.000004 1 ATTCGCA 20 7.8551704E-4 44.000004 20 AAGCGTG 20 7.8551704E-4 44.000004 34 CGCTGTT 20 7.8551704E-4 44.000004 17 TGGCCAT 20 7.8551704E-4 44.000004 44 TGTTGGC 20 7.8551704E-4 44.000004 11 ACGATTG 20 7.8551704E-4 44.000004 1 GTTACGA 20 7.8551704E-4 44.000004 9 GGCCACT 20 7.8551704E-4 44.000004 8 CCGTAGG 20 7.8551704E-4 44.000004 2 TACGCGG 20 7.8551704E-4 44.000004 2 GGGACGT 40 8.307325E-9 44.000004 8 >>END_MODULE