FastQCFastQC Report
Thu 26 May 2016
SRR1546254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546254_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences844407
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114531.3563364586034934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14810.17538935608065778No Hit
AAGCTTGGGCAAGAGTTACAAAAATACACTCTGACATGGGAAGCAGTTTA13280.15727013158346625No Hit
GAGTGAGGGATTTATTAGTTGATTTTATTGTTTAGAAAGGCACATGCATG11230.13299273928330768No Hit
GCAATAGGGATATCTTATCATTTGGTTTTTAATATTCGATAAATCCATAA11120.13169004994037237No Hit
TGGTAAGGGAACAGAATTTAACTGGATCGCAGACAGTTATTACTGTAATT10570.12517660322569568No Hit
TTTAATGGGTATCAACAATAATTTTAAATAATCTAAGTATAGGTAATATA10390.12304492975543785No Hit
GGATTGGGGATAACATTTTAAATGTAAATAAAGAAAATATCCAACAAAAA10310.12209751932421213No Hit
GTTGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT10290.12186066671640572No Hit
TATTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT10250.12138696150079287No Hit
CTGCAAGGGATGAATAATTATATTTTTGTTTTAAGTTCATTATTTTTGGT10120.11984741955005111No Hit
TGGTTTGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA9980.11818945129540613No Hit
GGGTTTGGGAAGTGTATACTTTATCTTCACATGTGGGTGGAAGGCATTGA9820.11629463043295472No Hit
ACTGGAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA9740.11534722000172903No Hit
TAAGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT9670.11451823587440653No Hit
TGGAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA9660.11439980957050333No Hit
TTGGTAGGGCATATTTTAAAATTTAGAAGAAAACTTAGAGAAAACTCCAT9580.11345239913927761No Hit
TCTTTAGGGCGATTGGAATGGGTTTTCAAAACAATCACCATCATGCTATT9470.11214970979634228No Hit
GACTGAGGGACTGCTAAACTGTAAAATTCAGGAATTAAAATGTGACCCTA9320.11037331523779409No Hit
AAATTGGGGAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGG9230.10930747850266519No Hit
GTTACTGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA9210.10907062589485875No Hit
TGTAGAGGGAGCTGTTAAGAGCAAGTATTTAATTCAGGTATTTTTCAAGT8990.10646524720898808No Hit
ATGACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA8930.10575468938556881No Hit
TTTTGAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC8920.1056362630816656No Hit
ATGAGAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA8870.10504413156214953No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC8660.10255717918018206No Hit
GTTAAGGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC8650.10243875287627886No Hit
TAAACCGGGAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCAT8620.10208347396456921No Hit
TTGTAGGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT8510.10078078462163388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG950.044.0000041
TTTACGG950.044.0000042
CCTAGGA950.044.00000439
CGGACAA302.5269164E-644.038
TCACGAC502.7284841E-1144.025
GTCATAA207.8552944E-444.020
ATCCTAA2300.044.025
AAGTAGT254.4418328E-544.014
CGGGTAT800.044.06
CGGGTAC254.4418328E-544.06
AGCGTCA207.8552944E-444.025
AGGTACG408.307325E-944.01
ACTATCA351.4454781E-744.034
CTCACGA502.7284841E-1144.024
AGTCCCT408.307325E-944.017
GTTCTAA800.044.021
GTCGATG502.7284841E-1144.01
CACGGTA351.4454781E-744.028
CAAGTAC207.8552944E-444.029
ACTGATC351.4454781E-744.041
Produced by FastQC (version 0.11.5)