##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546254_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	844407
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.805872049852738	31.0	31.0	34.0	30.0	34.0
2	32.20498882647823	33.0	31.0	34.0	30.0	34.0
3	32.21327274643625	33.0	31.0	34.0	30.0	34.0
4	35.90531817002938	37.0	35.0	37.0	35.0	37.0
5	35.84430020120629	37.0	35.0	37.0	35.0	37.0
6	35.91967262232549	37.0	35.0	37.0	35.0	37.0
7	36.15690301004137	37.0	35.0	37.0	35.0	37.0
8	36.05732425240435	37.0	35.0	37.0	35.0	37.0
9	37.8382746708637	39.0	38.0	39.0	35.0	39.0
10	37.50392760836895	39.0	37.0	39.0	35.0	39.0
11	37.276337121790796	39.0	37.0	39.0	34.0	39.0
12	36.52741628148512	39.0	35.0	39.0	33.0	39.0
13	36.249492247222015	39.0	35.0	39.0	32.0	39.0
14	37.37014022858645	40.0	35.0	41.0	33.0	41.0
15	37.62624540061842	40.0	35.0	41.0	33.0	41.0
16	37.749965360306106	40.0	35.0	41.0	33.0	41.0
17	37.69481067778926	40.0	35.0	41.0	33.0	41.0
18	37.529621379263794	39.0	36.0	41.0	33.0	41.0
19	37.262336764143356	38.0	35.0	41.0	33.0	41.0
20	36.883032708160876	37.0	35.0	41.0	33.0	41.0
21	36.80489148005642	37.0	35.0	41.0	33.0	41.0
22	36.73756020497225	37.0	35.0	41.0	33.0	41.0
23	36.69358496554387	37.0	35.0	41.0	33.0	41.0
24	36.656273574236124	37.0	35.0	41.0	33.0	41.0
25	36.59723450895125	37.0	35.0	41.0	33.0	41.0
26	36.52706810815164	37.0	35.0	41.0	33.0	41.0
27	36.452973506851556	37.0	35.0	41.0	32.0	41.0
28	36.39111589553379	37.0	35.0	41.0	32.0	41.0
29	36.31160565935621	37.0	35.0	41.0	32.0	41.0
30	36.10539704194778	37.0	35.0	40.0	31.0	41.0
31	35.73423479435864	37.0	35.0	40.0	30.0	41.0
32	35.29300917685429	37.0	35.0	41.0	29.0	41.0
33	34.80772660577186	37.0	35.0	41.0	23.0	41.0
34	34.354732966448644	36.0	34.0	40.0	21.0	41.0
35	34.04984918410198	36.0	34.0	40.0	18.0	41.0
36	33.847291649642884	36.0	34.0	40.0	18.0	41.0
37	33.73398136206829	36.0	33.0	40.0	17.0	41.0
38	33.701685324730846	36.0	33.0	40.0	17.0	41.0
39	33.63489999490767	36.0	33.0	40.0	16.0	41.0
40	33.51123687984585	36.0	33.0	40.0	15.0	41.0
41	33.42871624702306	36.0	33.0	40.0	15.0	41.0
42	33.31849925450642	36.0	33.0	40.0	14.0	41.0
43	33.210843822943204	35.0	33.0	40.0	12.0	41.0
44	33.05293655784474	35.0	33.0	40.0	10.0	41.0
45	33.00265985478567	35.0	33.0	40.0	10.0	41.0
46	32.95970545009693	35.0	33.0	40.0	10.0	41.0
47	32.906644544633096	35.0	33.0	40.0	10.0	41.0
48	32.84195062333685	35.0	33.0	40.0	10.0	41.0
49	32.79521131397537	36.0	33.0	40.0	10.0	41.0
50	32.5717195617753	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	2.0
13	3.0
14	5.0
15	14.0
16	46.0
17	159.0
18	350.0
19	764.0
20	1351.0
21	2277.0
22	3559.0
23	5390.0
24	8663.0
25	14439.0
26	22280.0
27	27029.0
28	25729.0
29	22523.0
30	20896.0
31	21487.0
32	25500.0
33	33140.0
34	62959.0
35	128814.0
36	43877.0
37	53852.0
38	91915.0
39	227380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.476797326407766	24.51021841363229	27.50202212913915	20.5109621308208
2	37.24661211951109	22.29990987758273	29.330050556189136	11.123427446717045
3	25.211538985347115	23.712261977932442	40.2699172318562	10.806281804864241
4	21.344683310299416	24.812797620104995	41.99763857950017	11.844880490095417
5	19.514760062386976	29.656196597138585	38.60685664614339	12.222186694331052
6	16.44893990694061	39.916888419920724	35.324671633465854	8.309500039672812
7	77.47401430826604	3.6983350445934247	17.37645471911057	1.4511959280299667
8	77.86943973699887	3.137941774523423	16.749979571462575	2.242638917015136
9	75.31439223028705	3.926897811126625	18.1331988010521	2.6255111575342225
10	43.43722872974762	24.13871509828791	23.189646698807564	9.234409473156902
11	37.33472128961508	19.33475208045409	31.754710702303512	11.575815927627318
12	33.99249414085862	18.720356415804225	36.2150005862102	11.072148857126955
13	15.914955702641025	38.09004425590977	34.599665800970385	11.395334240478821
14	10.728475723199832	39.17589503639832	38.23298480472095	11.862644435680899
15	9.685258412116433	21.106409586846155	57.28576385558149	11.922568145455923
16	10.908838984044424	18.420737866929098	52.90102995356505	17.76939319546143
17	10.816940172215531	20.112576044490392	38.35046369819293	30.72002008510114
18	14.76681268629938	23.48760727942805	45.35147150603915	16.394108528233424
19	19.537971617952007	24.45432119818997	36.89630711256539	19.111400071292636
20	22.404835582840978	21.08154006302648	39.157657385597226	17.355966968535316
21	15.92549564368841	26.061129289548763	39.97243035645133	18.040944710311496
22	18.062261445014073	21.872746199403842	34.22816248562601	25.836829869956073
23	12.494330340700634	26.793832831797936	35.266524318249374	25.445312509252055
24	13.319406399994316	21.14785879321228	51.339105431385576	14.193629375407832
25	12.062074331453907	25.328662599907393	44.97061251268642	17.638650555952285
26	12.97419372411645	30.06299095104612	38.11763758471922	18.845177740118213
27	12.264820163736207	34.477213002734466	35.02244770590485	18.235519127624475
28	11.234511319778258	28.80423776685887	41.342741118915406	18.618509794447462
29	11.076885909283082	23.207647497000856	41.33456970394608	24.380896889769982
30	13.514454522522906	30.907252071572124	38.60981730374097	16.968476102164004
31	18.756713291102514	31.036455169130527	33.633188734816265	16.573642804950694
32	20.23266031664825	24.70526653616088	37.093131629652525	17.968941517538344
33	22.014857764087697	28.831002111541	31.838911804378693	17.31522831999261
34	15.488739434893365	26.806622872619485	33.293897374133564	24.41074031835359
35	16.155716378476257	26.619035607236796	35.87866988312508	21.34657813116187
36	24.474453669853517	26.851624868102704	31.593177223779527	17.080744238264252
37	15.445632260272593	32.15605744623149	36.659336078455055	15.738974215040852
38	16.532904156407987	28.459143517284915	34.036075020694994	20.971877305612104
39	16.268576646096015	31.680457409756198	34.35795771470393	17.69300822944386
40	18.366024914525816	25.6238993755381	34.00469205016064	22.00538365977544
41	12.798804368035793	26.80934667760926	36.194394409331046	24.197454545023906
42	16.871366532963368	27.7031099931668	32.12206909701128	23.303454376858554
43	17.941821893944507	25.35897973370661	34.5042142000244	22.19498417232448
44	14.726192464060578	27.970161308468544	35.60072334786424	21.702922879606636
45	14.706533697612645	35.65283092158165	30.288474633677836	19.35216074712787
46	20.00705820771263	29.441608134465962	33.169431328731285	17.381902329090117
47	17.629176451640028	28.94102014786708	32.277562834036196	21.152240566456697
48	17.25471247869807	27.17031005190625	34.44831698458208	21.126660484813602
49	19.15178344092363	24.97219942515872	36.254318119106074	19.62169901481158
50	18.184240538034384	30.426322851421173	32.87443140570838	18.515005204836058
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2002.0
1	2557.5
2	3113.0
3	11796.0
4	20479.0
5	15316.0
6	10153.0
7	11322.0
8	12491.0
9	13343.0
10	14195.0
11	14088.5
12	13982.0
13	13301.0
14	12620.0
15	11761.0
16	10902.0
17	10069.5
18	9237.0
19	8104.5
20	6972.0
21	6825.0
22	6678.0
23	7837.5
24	8997.0
25	9889.0
26	10781.0
27	13133.0
28	15485.0
29	19016.5
30	22548.0
31	24434.5
32	26321.0
33	31488.0
34	36655.0
35	39593.0
36	42531.0
37	46748.0
38	50965.0
39	53660.5
40	56356.0
41	69003.5
42	81651.0
43	77279.5
44	72908.0
45	69210.0
46	65512.0
47	62361.0
48	59210.0
49	53458.5
50	47707.0
51	40358.5
52	33010.0
53	27522.5
54	22035.0
55	18901.0
56	15767.0
57	13902.0
58	12037.0
59	11534.5
60	11032.0
61	10655.0
62	10278.0
63	8829.5
64	7381.0
65	5789.0
66	4197.0
67	3620.5
68	3044.0
69	2579.5
70	2115.0
71	1704.0
72	1293.0
73	1045.0
74	797.0
75	641.5
76	486.0
77	384.5
78	283.0
79	213.5
80	144.0
81	85.5
82	27.0
83	20.0
84	13.0
85	11.0
86	9.0
87	6.5
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	844407.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.393660708194744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.58743278256574	20.210162647167298
2	8.552002582937012	4.343333039334151
3	3.2038426466430896	2.440718793938978
4	1.629461840708915	1.6551200447965062
5	1.0101108146832263	1.2825205652872012
6	0.6640553152689719	1.0117677220448138
7	0.46880050246085436	0.8333192629525504
8	0.35041829353330844	0.7118722601543541
9	0.2755762691916555	0.6298101251174735
>10	3.095037609985072	18.224536993536006
>50	0.648390534579056	11.387504946505143
>100	0.4455342473870729	23.439082736812047
>500	0.06418316707889483	11.130893494657174
>1k	0.004684902706488674	1.336830266231979
>5k	0.0	0.0
>10k+	4.684902706488675E-4	1.3625271014643459
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11453	1.3563364586034934	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1481	0.17538935608065778	No Hit
AAGCTTGGGCAAGAGTTACAAAAATACACTCTGACATGGGAAGCAGTTTA	1328	0.15727013158346625	No Hit
GAGTGAGGGATTTATTAGTTGATTTTATTGTTTAGAAAGGCACATGCATG	1123	0.13299273928330768	No Hit
GCAATAGGGATATCTTATCATTTGGTTTTTAATATTCGATAAATCCATAA	1112	0.13169004994037237	No Hit
TGGTAAGGGAACAGAATTTAACTGGATCGCAGACAGTTATTACTGTAATT	1057	0.12517660322569568	No Hit
TTTAATGGGTATCAACAATAATTTTAAATAATCTAAGTATAGGTAATATA	1039	0.12304492975543785	No Hit
GGATTGGGGATAACATTTTAAATGTAAATAAAGAAAATATCCAACAAAAA	1031	0.12209751932421213	No Hit
GTTGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1029	0.12186066671640572	No Hit
TATTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1025	0.12138696150079287	No Hit
CTGCAAGGGATGAATAATTATATTTTTGTTTTAAGTTCATTATTTTTGGT	1012	0.11984741955005111	No Hit
TGGTTTGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA	998	0.11818945129540613	No Hit
GGGTTTGGGAAGTGTATACTTTATCTTCACATGTGGGTGGAAGGCATTGA	982	0.11629463043295472	No Hit
ACTGGAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	974	0.11534722000172903	No Hit
TAAGCAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	967	0.11451823587440653	No Hit
TGGAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	966	0.11439980957050333	No Hit
TTGGTAGGGCATATTTTAAAATTTAGAAGAAAACTTAGAGAAAACTCCAT	958	0.11345239913927761	No Hit
TCTTTAGGGCGATTGGAATGGGTTTTCAAAACAATCACCATCATGCTATT	947	0.11214970979634228	No Hit
GACTGAGGGACTGCTAAACTGTAAAATTCAGGAATTAAAATGTGACCCTA	932	0.11037331523779409	No Hit
AAATTGGGGAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGG	923	0.10930747850266519	No Hit
GTTACTGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	921	0.10907062589485875	No Hit
TGTAGAGGGAGCTGTTAAGAGCAAGTATTTAATTCAGGTATTTTTCAAGT	899	0.10646524720898808	No Hit
ATGACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	893	0.10575468938556881	No Hit
TTTTGAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	892	0.1056362630816656	No Hit
ATGAGAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	887	0.10504413156214953	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC	866	0.10255717918018206	No Hit
GTTAAGGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC	865	0.10243875287627886	No Hit
TAAACCGGGAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCAT	862	0.10208347396456921	No Hit
TTGTAGGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT	851	0.10078078462163388	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08846444901569978	0.0
2	0.0	0.0	0.0	0.38855670310644036	0.0
3	0.0	0.0	0.0	0.5896445671340953	0.0
4	0.0	0.0	0.0	0.9603188983511506	0.0
5	0.0	0.0	0.0	1.6789297104358443	0.0
6	0.0	0.0	0.0	2.283969697077357	0.0
7	0.0	0.0	0.0	2.6285902414357056	0.0
8	0.0	0.0	0.0	3.5109846318185425	0.0
9	0.0	0.0	0.0	3.8174719063200566	0.0
10	0.0	0.0	0.0	4.417419561893731	0.0
11	0.0	0.0	0.0	5.0521845508149505	0.0
12	0.0	0.0	0.0	5.622999335628435	0.0
13	0.0	0.0	0.0	5.830126941155154	0.0
14	0.0	0.0	0.0	5.8927744559199535	0.0
15	0.0	0.0	0.0	6.006226855059231	0.0
16	0.0	0.0	0.0	6.275765122742943	0.0
17	0.0	0.0	0.0	6.6066482158485185	0.0
18	0.0	0.0	0.0	7.160528039203844	0.0
19	0.0	0.0	0.0	7.383287916845786	0.0
20	0.0	0.0	0.0	7.6602870416754	0.0
21	0.0	0.0	0.0	8.004789159729846	0.0
22	0.0	0.0	0.0	8.311986992054779	0.0
23	0.0	0.0	0.0	8.686806243908446	0.0
24	0.0	0.0	0.0	8.947462538799417	0.0
25	0.0	0.0	0.0	9.162998411903265	0.0
26	0.0	0.0	0.0	9.403048529915077	0.0
27	0.0	0.0	0.0	9.605083804373956	0.0
28	0.0	0.0	0.0	9.870121872509348	0.0
29	0.0	0.0	0.0	10.111593106167998	0.0
30	0.0	0.0	0.0	10.463792933976151	0.0
31	0.0	0.0	0.0	10.818124435254564	0.0
32	0.0	0.0	0.0	11.094886707476372	0.0
33	0.0	0.0	0.0	11.372359537521598	0.0
34	0.0	0.0	0.0	11.63123943785402	0.0
35	0.0	0.0	0.0	12.016243351843364	0.0
36	0.0	0.0	0.0	12.314440785071653	0.0
37	0.0	0.0	0.0	12.618914812406814	0.0
38	0.0	0.0	0.0	12.864057261486463	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	95	0.0	44.000004	1
TTTACGG	95	0.0	44.000004	2
CCTAGGA	95	0.0	44.000004	39
CGGACAA	30	2.5269164E-6	44.0	38
TCACGAC	50	2.7284841E-11	44.0	25
GTCATAA	20	7.8552944E-4	44.0	20
ATCCTAA	230	0.0	44.0	25
AAGTAGT	25	4.4418328E-5	44.0	14
CGGGTAT	80	0.0	44.0	6
CGGGTAC	25	4.4418328E-5	44.0	6
AGCGTCA	20	7.8552944E-4	44.0	25
AGGTACG	40	8.307325E-9	44.0	1
ACTATCA	35	1.4454781E-7	44.0	34
CTCACGA	50	2.7284841E-11	44.0	24
AGTCCCT	40	8.307325E-9	44.0	17
GTTCTAA	80	0.0	44.0	21
GTCGATG	50	2.7284841E-11	44.0	1
CACGGTA	35	1.4454781E-7	44.0	28
CAAGTAC	20	7.8552944E-4	44.0	29
ACTGATC	35	1.4454781E-7	44.0	41
>>END_MODULE