FastQCFastQC Report
Thu 26 May 2016
SRR1546253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546253_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1198140
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200841.6762648772263675No Hit
ATGTGAGGGGCTGGCAAGGATTTTAATGATACAGTAAAGATAAACATATA22730.1897107182800007No Hit
AGCGTTGGGAGTCTTATTAAAGACACGAAATTTACAAAGTTCTTAAAGAC17200.14355584489291737No Hit
GGGTATTTTAATATAAATATTTTTCTTATAATTTTCTTTCTTCTATCCAT16590.13846462016124994No Hit
GAGGAAGGGTCATATTCTTCCCTAGGAAGTGAAAAGAGAAAACAGTAACA15920.1328726192264677No Hit
AAAGACGGGATTTATATTTTTGATAGTAGACTATTCTTAATATAAAGTTG15400.1285325587994725No Hit
TTGCAAGGGAGTTGAGAGATTCAAGATACAGGCAAAGTCCACAGAACAAA15180.12669637938805148No Hit
TTTTTTGGGATGTAATTTAATGCAGTTAAAGTATGAAAGTAGAATGTGTG14800.12352479676832424No Hit
TTAGCAGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAAAGGAA14030.11709816882835061No Hit
TAAGCAGAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGA14020.11701470612783148No Hit
AGATGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13930.11626354182315923No Hit
TTTTTTGGGAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAA13460.11234079489875974No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13230.11042115278681956No Hit
TATGAGGGGATGGTTTACAGGTAATATTCGCATTTTAGACCAATTAAGCT13040.10883536147695595No Hit
GGACTTGGGGATTATATTTTCTTTAATTTGTAAAATGCACCCATATTCTA12770.10658186856293923No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12570.10491261455255645No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC12470.10407798754736508No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.10341028594321197No Hit
TTTTGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT12180.10165756923231008No Hit
GTTATAGGGAAGTGTGTAGTTAATATTATGCATGTTATTGTCCTTTCTTC12090.10090640492763783No Hit
AAATAAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG12070.10073947952659956No Hit
ACTATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT12040.10048909142504214No Hit
GAGAGTGTTCCATAGCATTTTAAATATTCCCCAAAATGAATCATGTTTCA12010.10023870332348474No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG900.044.00000437
GCATTAC650.044.0000049
GAAACGT454.802132E-1044.00000421
GTATCGA900.044.00000438
TGCGTTG454.802132E-1044.0000041
AACCGTG800.044.044
CTAGACG254.442954E-544.01
CTGTCGA207.856615E-444.027
TCGTTAG302.5278077E-644.01
GTCGAGA207.856615E-444.029
CGAGTGT207.856615E-444.028
CACGGCA207.856615E-444.039
CTATCGA207.856615E-444.029
TTTCGTA408.312782E-944.030
CGTTGTA254.442954E-544.08
CATTGAC302.5278077E-644.019
CGGCACG207.856615E-444.022
CTCCCGA207.856615E-444.030
CCAATCG254.442954E-544.03
GTTAGCG1550.044.01
Produced by FastQC (version 0.11.5)