##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546253_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1198140 Sequences flagged as poor quality 0 Sequence length 50 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.886388068172334 31.0 31.0 34.0 30.0 34.0 2 32.21759811040446 33.0 31.0 34.0 30.0 34.0 3 32.10817350226184 33.0 31.0 34.0 30.0 34.0 4 35.87241975061345 37.0 35.0 37.0 35.0 37.0 5 35.8586166892016 37.0 35.0 37.0 35.0 37.0 6 35.91717829302085 37.0 35.0 37.0 35.0 37.0 7 36.186757807935635 37.0 35.0 37.0 35.0 37.0 8 36.09172717712454 37.0 35.0 37.0 35.0 37.0 9 37.84234313185438 39.0 38.0 39.0 35.0 39.0 10 37.47197239053867 39.0 37.0 39.0 35.0 39.0 11 37.31798370808086 39.0 37.0 39.0 34.0 39.0 12 36.57467908591651 39.0 35.0 39.0 33.0 39.0 13 36.294849516750965 39.0 35.0 39.0 33.0 39.0 14 37.20941793112658 40.0 35.0 41.0 32.0 41.0 15 37.489711552907 40.0 35.0 41.0 33.0 41.0 16 37.64158111739864 40.0 35.0 41.0 33.0 41.0 17 37.59529103443671 40.0 35.0 41.0 33.0 41.0 18 37.41244846178243 39.0 35.0 41.0 33.0 41.0 19 37.1929098435909 38.0 35.0 41.0 33.0 41.0 20 36.8940357554209 38.0 35.0 41.0 33.0 41.0 21 36.79801108384663 38.0 35.0 41.0 33.0 41.0 22 36.72316006476706 37.0 35.0 41.0 33.0 41.0 23 36.69219874138248 37.0 35.0 41.0 33.0 41.0 24 36.632194902098256 37.0 35.0 41.0 33.0 41.0 25 36.61628941526032 37.0 35.0 41.0 33.0 41.0 26 36.563848965897144 37.0 35.0 41.0 33.0 41.0 27 36.48969903350193 37.0 35.0 41.0 32.0 41.0 28 36.41795366150867 37.0 35.0 41.0 32.0 41.0 29 36.325555444271956 37.0 35.0 41.0 32.0 41.0 30 36.09773899544294 37.0 35.0 40.0 31.0 41.0 31 35.72181965379672 37.0 35.0 40.0 30.0 41.0 32 35.36804630510625 37.0 35.0 40.0 29.0 41.0 33 34.95093728612683 37.0 35.0 40.0 24.0 41.0 34 34.47596274225049 37.0 34.0 40.0 22.0 41.0 35 34.23332164855526 37.0 34.0 40.0 20.0 41.0 36 34.094007378102724 36.0 34.0 40.0 18.0 41.0 37 34.02315839551304 36.0 34.0 40.0 18.0 41.0 38 33.96133089622248 36.0 34.0 40.0 18.0 41.0 39 33.9078939022151 36.0 34.0 40.0 18.0 41.0 40 33.74456157043417 36.0 33.0 40.0 18.0 41.0 41 33.653302619059545 36.0 34.0 40.0 17.0 41.0 42 33.62020214666066 36.0 33.0 40.0 17.0 41.0 43 33.50303136528286 36.0 33.0 40.0 16.0 41.0 44 33.37344467257583 35.0 33.0 40.0 15.0 41.0 45 33.2986328809655 35.0 33.0 40.0 15.0 41.0 46 33.28892116113309 35.0 33.0 40.0 15.0 41.0 47 33.25151317876041 35.0 33.0 40.0 15.0 41.0 48 33.1647587093328 35.0 33.0 40.0 15.0 41.0 49 33.1142445790976 35.0 33.0 40.0 15.0 41.0 50 32.933717261755724 35.0 33.0 40.0 15.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 2.0 11 4.0 12 2.0 13 7.0 14 15.0 15 33.0 16 81.0 17 207.0 18 501.0 19 1018.0 20 1844.0 21 3102.0 22 4616.0 23 7227.0 24 11454.0 25 19275.0 26 29448.0 27 35857.0 28 34201.0 29 29881.0 30 29038.0 31 30982.0 32 37130.0 33 48679.0 34 94731.0 35 180577.0 36 62826.0 37 78944.0 38 135678.0 39 320778.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.45054834994241 22.240472732735743 25.829452317759195 19.479526599562654 2 38.37080808586643 22.439197422671807 29.34623666683359 9.843757824628174 3 26.91989250004173 23.265060844308678 39.12731400337189 10.687732652277697 4 23.896623099136995 25.528819670489256 38.622781978733705 11.951775251640044 5 20.152569816548986 30.105914166958787 38.01617507136061 11.72534094513162 6 15.820104495301049 40.06142854758208 36.13818084697948 7.98028611013738 7 79.05036139349325 3.3967649857278785 16.331063147879213 1.2218104728996613 8 78.86332148162985 3.087619143004991 16.159129984809788 1.889929390555361 9 75.94446391907456 4.167042248819003 17.702021466606574 2.186472365499858 10 43.85639407748677 24.37536514931477 23.31722503213314 8.451015741065317 11 38.223412956749634 19.892249653629793 29.543292102759278 12.341045286861302 12 35.03638973742634 20.366735106081094 34.00228687799422 10.594588278498339 13 19.92171198691305 34.778740380923765 32.96793363046055 12.331614001702638 14 13.33266563172918 36.262957584255595 38.09496386065068 12.30941292336455 15 11.536214465755254 22.228120253058908 54.74176640459378 11.493898876592052 16 15.259735924015558 20.168928505850737 48.716760979518256 15.854574590615453 17 15.296793363046055 20.618792461648887 35.6829752783481 28.40143889695695 18 16.940674712470997 22.882300899727912 44.537449713722935 15.639574674078155 19 21.290249887325356 23.72577495117432 36.162218104729 18.82175705677133 20 22.485853072262003 23.385497521157795 38.142370674545546 15.986278732034654 21 19.208272822875458 25.025789974460416 40.00400620962492 15.761930993039211 22 19.591199692857263 21.821072662627074 34.336221142771294 24.251506501744373 23 14.60689068055486 26.311950189460333 35.88779274542207 23.19336638456274 24 14.105613701236916 25.196888510524644 46.3462533593737 14.35124442886474 25 14.993239521257951 24.966364531690786 42.64384796434474 17.39654798270653 26 14.67850167760028 28.148296526282408 36.39332632246649 20.779875473650826 27 13.943111823326154 32.039077236383065 37.479342981621514 16.53846795866927 28 13.005575308394679 29.942327273941277 39.96035521725341 17.091742200410636 29 14.22354649707046 24.621246265044153 39.280718446926066 21.87448879095932 30 16.97714791259786 29.753868496169062 36.9812375849233 16.28774600630978 31 21.01198524379455 29.93214482447794 33.23960472065034 15.816265211077168 32 23.126846612248986 24.787003188275158 35.102074882734904 16.98407531674095 33 22.676565342948237 30.047991052798505 31.477206336488223 15.798237267765035 34 17.674228387333702 28.16899527601115 33.41612833224832 20.740648004406832 35 17.853756656150367 27.771796284240573 33.733370056921565 20.6410770026875 36 27.111940173936265 27.804597125544596 30.278515031632363 14.804947668886776 37 16.912631245096566 33.44609144173469 34.26486053382744 15.376416779341312 38 18.083446007979035 30.403458694309514 32.126629609227635 19.386465688483817 39 17.77304822474836 30.564040930108334 33.66409601549068 17.99881482965263 40 20.100572554125563 26.58111739863455 32.55696329310431 20.761346754135577 41 14.816715909659973 26.567095664947338 35.75900979852104 22.85717862687165 42 17.770377418331748 27.169779825396027 32.60478742050178 22.455055335770442 43 17.933463535146142 26.047790742317257 32.86928071844692 23.149465004089674 44 16.453836780342865 28.63930759343649 33.46261705643748 21.444238569783163 45 16.961206536798702 35.41940007010867 28.7858680955481 18.833525297544526 46 20.421653563022684 31.4177808937186 32.060527150416476 16.10003839284224 47 17.55713021850535 30.424908608342932 32.89239988649072 19.125561286660993 48 18.382409401238586 29.481279316273557 33.73779358004907 18.39851770243878 49 21.41085348957551 26.125995292703692 34.303336838766754 18.159814378954046 50 18.706077753851805 30.42674478775435 31.219473517285124 19.64770394110872 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2555.0 1 4351.0 2 6147.0 3 19640.5 4 33134.0 5 23793.5 6 14453.0 7 14728.0 8 15003.0 9 16116.5 10 17230.0 11 17138.0 12 17046.0 13 16219.0 14 15392.0 15 13721.0 16 12050.0 17 11504.0 18 10958.0 19 9836.5 20 8715.0 21 9048.0 22 9381.0 23 11233.5 24 13086.0 25 13126.0 26 13166.0 27 15931.0 28 18696.0 29 21807.5 30 24919.0 31 30307.0 32 35695.0 33 50060.5 34 64426.0 35 65243.0 36 66060.0 37 69214.0 38 72368.0 39 75127.5 40 77887.0 41 87069.0 42 96251.0 43 97230.0 44 98209.0 45 93271.5 46 88334.0 47 82199.5 48 76065.0 49 71878.5 50 67692.0 51 58843.0 52 49994.0 53 42592.0 54 35190.0 55 32320.0 56 29450.0 57 28263.0 58 27076.0 59 24865.5 60 22655.0 61 20339.0 62 18023.0 63 15934.0 64 13845.0 65 11416.0 66 8987.0 67 7897.5 68 6808.0 69 5552.5 70 4297.0 71 3422.5 72 2548.0 73 2179.0 74 1810.0 75 1488.5 76 1167.0 77 894.5 78 622.0 79 525.5 80 429.0 81 320.5 82 212.0 83 141.0 84 70.0 85 43.5 86 17.0 87 15.0 88 13.0 89 9.0 90 5.0 91 3.5 92 2.0 93 2.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1198140.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.122039205610616 #Duplication Level Percentage of deduplicated Percentage of total 1 78.97030581691895 19.04924812994779 2 8.687314010422202 4.191114583017094 3 3.25385636937681 2.3546895273459967 4 1.7691450760321568 1.7070154753784261 5 1.0483372756019722 1.2644016431386895 6 0.739372504651991 1.0701103526859503 7 0.534950849924609 0.9032873762469295 8 0.4158531083493301 0.8024979986702063 9 0.3094713833188438 0.671857275728853 >10 3.000793942239434 16.5909033762255 >50 0.7364888111278377 12.161223974481478 >100 0.45274240192101406 21.86839412346458 >500 0.06572067124659868 11.110469170336225 >1k 0.015300050448943607 4.570157429115412 >5k 0.0 0.0 >10k+ 3.477284192941729E-4 1.6846295642168292 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20084 1.6762648772263675 No Hit ATGTGAGGGGCTGGCAAGGATTTTAATGATACAGTAAAGATAAACATATA 2273 0.1897107182800007 No Hit AGCGTTGGGAGTCTTATTAAAGACACGAAATTTACAAAGTTCTTAAAGAC 1720 0.14355584489291737 No Hit GGGTATTTTAATATAAATATTTTTCTTATAATTTTCTTTCTTCTATCCAT 1659 0.13846462016124994 No Hit GAGGAAGGGTCATATTCTTCCCTAGGAAGTGAAAAGAGAAAACAGTAACA 1592 0.1328726192264677 No Hit AAAGACGGGATTTATATTTTTGATAGTAGACTATTCTTAATATAAAGTTG 1540 0.1285325587994725 No Hit TTGCAAGGGAGTTGAGAGATTCAAGATACAGGCAAAGTCCACAGAACAAA 1518 0.12669637938805148 No Hit TTTTTTGGGATGTAATTTAATGCAGTTAAAGTATGAAAGTAGAATGTGTG 1480 0.12352479676832424 No Hit TTAGCAGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAAAGGAA 1403 0.11709816882835061 No Hit TAAGCAGAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGA 1402 0.11701470612783148 No Hit AGATGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 1393 0.11626354182315923 No Hit TTTTTTGGGAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAA 1346 0.11234079489875974 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1323 0.11042115278681956 No Hit TATGAGGGGATGGTTTACAGGTAATATTCGCATTTTAGACCAATTAAGCT 1304 0.10883536147695595 No Hit GGACTTGGGGATTATATTTTCTTTAATTTGTAAAATGCACCCATATTCTA 1277 0.10658186856293923 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1257 0.10491261455255645 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC 1247 0.10407798754736508 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1239 0.10341028594321197 No Hit TTTTGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 1218 0.10165756923231008 No Hit GTTATAGGGAAGTGTGTAGTTAATATTATGCATGTTATTGTCCTTTCTTC 1209 0.10090640492763783 No Hit AAATAAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG 1207 0.10073947952659956 No Hit ACTATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 1204 0.10048909142504214 No Hit GAGAGTGTTCCATAGCATTTTAAATATTCCCCAAAATGAATCATGTTTCA 1201 0.10023870332348474 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.09414592618558766 0.0 2 0.0 0.0 0.0 0.3389420268082194 0.0 3 0.0 0.0 0.0 0.4794932144824478 0.0 4 0.0 0.0 0.0 0.7179461498656251 0.0 5 0.0 0.0 0.0 1.2098753067254244 0.0 6 0.0 0.0 0.0 1.6356185420735474 0.0 7 0.0 0.0 0.0 1.8994441384145426 0.0 8 0.0 0.0 0.0 2.5163169579514917 0.0 9 0.0 0.0 0.0 2.7411654731500494 0.0 10 0.0 0.0 0.0 3.1790108000734474 0.0 11 0.0 0.0 0.0 3.7306157878044304 0.0 12 0.0 0.0 0.0 4.155524396147362 0.0 13 0.0 0.0 0.0 4.320363229672659 0.0 14 0.0 0.0 0.0 4.387383778189527 0.0 15 0.0 0.0 0.0 4.486954779908859 0.0 16 0.0 0.0 0.0 4.726158879596708 0.0 17 0.0 0.0 0.0 5.001251940507787 0.0 18 0.0 0.0 0.0 5.43575875941042 0.0 19 8.3462700519138E-5 0.0 0.0 5.621379805364983 0.0 20 8.3462700519138E-5 0.0 0.0 5.844475603852638 0.0 21 8.3462700519138E-5 0.0 0.0 6.143856310614786 0.0 22 8.3462700519138E-5 0.0 0.0 6.4367269267364415 0.0 23 8.3462700519138E-5 0.0 0.0 6.769743101807802 0.0 24 8.3462700519138E-5 0.0 0.0 7.015540754836663 0.0 25 8.3462700519138E-5 0.0 0.0 7.216769325788305 0.0 26 8.3462700519138E-5 0.0 0.0 7.44579097601282 0.0 27 8.3462700519138E-5 0.0 0.0 7.726726425960238 0.0 28 8.3462700519138E-5 0.0 0.0 7.968935182866777 0.0 29 8.3462700519138E-5 0.0 0.0 8.300449029328792 0.0 30 8.3462700519138E-5 0.0 0.0 8.65608359624084 0.0 31 8.3462700519138E-5 0.0 0.0 8.980002336955614 0.0 32 8.3462700519138E-5 0.0 0.0 9.246916053215818 0.0 33 8.3462700519138E-5 0.0 0.0 9.509823559851103 0.0 34 8.3462700519138E-5 0.0 0.0 9.811958535730382 0.0 35 8.3462700519138E-5 0.0 0.0 10.208406363196287 0.0 36 8.3462700519138E-5 0.0 0.0 10.478742050177775 0.0 37 8.3462700519138E-5 0.0 0.0 10.772196905203065 0.0 38 1.66925401038276E-4 0.0 0.0 11.132672308745223 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTATCG 90 0.0 44.000004 37 GCATTAC 65 0.0 44.000004 9 GAAACGT 45 4.802132E-10 44.000004 21 GTATCGA 90 0.0 44.000004 38 TGCGTTG 45 4.802132E-10 44.000004 1 AACCGTG 80 0.0 44.0 44 CTAGACG 25 4.442954E-5 44.0 1 CTGTCGA 20 7.856615E-4 44.0 27 TCGTTAG 30 2.5278077E-6 44.0 1 GTCGAGA 20 7.856615E-4 44.0 29 CGAGTGT 20 7.856615E-4 44.0 28 CACGGCA 20 7.856615E-4 44.0 39 CTATCGA 20 7.856615E-4 44.0 29 TTTCGTA 40 8.312782E-9 44.0 30 CGTTGTA 25 4.442954E-5 44.0 8 CATTGAC 30 2.5278077E-6 44.0 19 CGGCACG 20 7.856615E-4 44.0 22 CTCCCGA 20 7.856615E-4 44.0 30 CCAATCG 25 4.442954E-5 44.0 3 GTTAGCG 155 0.0 44.0 1 >>END_MODULE