FastQCFastQC Report
Thu 26 May 2016
SRR1546252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546252_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1240917
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210661.6976155536591084No Hit
ATGTGAGGGGCTGGCAAGGATTTTAATGATACAGTAAAGATAAACATATA23140.1864750019542No Hit
AGCGTTGGGAGTCTTATTAAAGACACGAAATTTACAAAGTTCTTAAAGAC17630.1420723545571541No Hit
GGGTATTTTAATATAAATATTTTTCTTATAATTTTCTTTCTTCTATCCAT17110.13788190507503725No Hit
TTTTTTGGGATGTAATTTAATGCAGTTAAAGTATGAAAGTAGAATGTGTG16020.1290980782759846No Hit
AAAGACGGGATTTATATTTTTGATAGTAGACTATTCTTAATATAAAGTTG15940.12845339374027434No Hit
GAGGAAGGGTCATATTCTTCCCTAGGAAGTGAAAAGAGAAAACAGTAACA15720.12668051126707103No Hit
TTGCAAGGGAGTTGAGAGATTCAAGATACAGGCAAAGTCCACAGAACAAA15660.12619699786528832No Hit
TAAGCAGAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGA14600.11765492776712706No Hit
TTAGCAGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAAAGGAA14590.11757434220016326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14160.11410916282072049No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13990.11273920818233613No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC13920.11217510921358961No Hit
AGATGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13540.10911285766896577No Hit
TTTTTTGGGAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAA13390.10790407416450898No Hit
GGACTTGGGGATTATATTTTCTTTAATTTGTAAAATGCACCCATATTCTA13070.10532533602166784No Hit
TATGAGGGGATGGTTTACAGGTAATATTCGCATTTTAGACCAATTAAGCT12970.10451948035202999No Hit
GAGAGTGTTCCATAGCATTTTAAATATTCCCCAAAATGAATCATGTTTCA12600.10153781437436991No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12500.10073195870473207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG900.044.00000437
GTATCGA900.044.00000438
CGTAAGG900.044.0000042
TGATGCG950.044.0000041
AACGTGT1100.044.035
AATCCGG207.856726E-444.02
TACCCGA207.856726E-444.040
ACACGAA2000.044.023
ATTAGGC207.856726E-444.027
ATAATCG207.856726E-444.01
CATTGCA207.856726E-444.025
TAGCGCG207.856726E-444.01
GCAAACC207.856726E-444.044
GTTAGCG1550.044.01
ATTCGAT302.5278805E-644.014
CGACATA502.7284841E-1144.040
GGATAGT1750.044.08
ATCACGT207.856726E-444.017
TTATCGG207.856726E-444.02
TCGATAG254.4430468E-544.01
Produced by FastQC (version 0.11.5)